Hemera Zoa
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Menelusuri asal usul sapi bali dengan menggunakan metoda analisis sitogenetik
Get PDFIn order to study the origin of Bali cattle, the cytogenetic analyses of several breed of cattle (the Bali, Madura, Rambon, Bali-Taurus and Bos-Taurus) were carried out. The relative length of chromosome pairs were compared each other, and the values obtained from them were analysed by t test.The Bali cattle have much more chromosome pairs wich differ with the Bos taurus than other local cattle breed. It means that the Bali cattle have the longest genetic distance in relation to Bos taurus compared with the other domestic cattle (Bos). Hitherto, it has been understood that the Bali cattle had been domesticatedfrom banteng
Kejadian penyakit gumoro pada ayam ras petelur
Get PDFTelah dilakukan penyidikan penyakit Gumboro di kabupaten Bangli Propinsi Bali dan Sikka, Flores Propinsi Nusa Tenggara Timur.Dalam penyidikan tersebut dilakukan pengamatan epidemiologis, gejala klinis, patologis, isolasi dan identifikasi agen penyebab.Dari gambaran epidemiologis menunjukkan penyakit menyerang ayam jenis AA Brown CP 909, Superharco CP 306 dan Logman pada umur 32,64 dan 58 hari denganmorbiditas masing-masing 100.0%, 5.97% dan 100.0%, mortalitas 38.48%, 2.24% dan 49.75% atau CFR 38.48%, 37.50% dan 49.75%. Tingkat mortalitas harian pada ayam AA Brown CP 909 menunjukkan paling tinggi terjadi pada hari ke lima sebesar 77.43% sedangkan pada ayam Superharco CP 306 terjadi pada hari ketiga dengan 19.89% dan pada jenis Logman terjadi pada hari ketiga dengan 1.01%. Kesembuhan terjadi pada ketiga jenis ayam tersebut masing-masing pada hari ke-12, ke-10 dan ke-5. Gejala klinis dan perubahan patologis ketiga jenis ayam adalah sama dan khas, serta virusnya mudah turnbuh pada telur ayam berembrio
Kajian morfologi spermatozoa sapi simmetal di beberapa Balai Inseminasi buatan Indonesia
No full textMorphological abnormality of sperm in semen of males have been associated with sub fertility and sterility for many years. This study to evaluated spermatozoa morphology especially primary abnormality of Simmental bull spermatozoa at several Artificial Insemination centre in Indonesia. One ejaculated were examined from each bull. Total of 70 bulls were tested in this study. One drop of semen was placed on each of 3-4 glass slides, and smears were prepared and air-dried. The spermatozoa were stained with carbolfluchsin-eosin (Williams stained). The types of morphological abnormalities were counted from 500 cells. Result demonstrated that from 70 bulls samples, 48 bulls (68.6%) were indicated low sperm abnormality (10%) were found in 6 bulls (8.57%). The highest type of abnormality found in all samples was pearshape (2.81±0.36%) and lowest were macrocephalus and double head (0.01±0.01%
Restriction Fragment Length Polymorphism (RFLP) gen sitokrom b dari delapan species burung
Get PDFThe aim of this research is to study the Restriction Fragment Length Polyorphism (RFLP) of eight species local birds of the cytochrome b mitochondrial DNA fiagment. The results gainedfiom this study is expected to be used as basic information for tissue birds species recognition. Eight bird I I tissues collected from Bogor area were preserved in 4,5 M NaCI containing 25% DMSO. DNA were isolated using ammonium acetat precipitation. Universal oligonucleotide cytochrome b primers L14841/H15149 were used for amplification of the mitochondrial cytochrome b DNA fragment using Polymerase Chain Reaction (PCR) method, followed by determination of the PCR product by electrophoresis. The amplicon were digested using restriction enzymes Hinf I (2,5 unit) and Rsa I (20 unit) for 2 and 6 hours, respectively. The calculation of the DNA amplicon and restriction fragment is done by measuring the distance between sample DNA migration with the DNA ladder in 2% agarose gel. The results showed that eight local birds species have 359 bp amplicon, and have different Hinf I restriction fragment size, except between scaly-breasted munia and streaked weaver, and also have difflerent Rsa I restriction fragment size except streaked weaver and javan turtle dove, and also chicken and yellow-vented bulbul. In conclusion, universal oligonucleotide cytochrome b primer followed by digestion with Hinf I and Rsa I restriction enzymes can be used for the eight birds species recognition.
Tingkat pertumbuhan dan analisa protein sel-sel fibroblas fetal tikus hasil kultur in vitro
No full textResearch has been conducted on in vitro growth of rat fetal fibroblast cells in Dulbecco S Modzfied Eagle Medium (DMEM) containing 10% Fetal Bovine Serum (FBS) and 50 µg/ml gentamycin. Culture was done in 5% CO2 incubator at 37°C for 10 days. Evaluation was done on the proliferation rate and the protein production after several passages. The growth of the fibroblast was confirmed by their morphology. The proliferation rate was done based on the population doubling time (PDT) by counting the number of cells in each passage by using Improved Neubauer hemocytometer. Protein secreted into the culture medium without FBS, designed as conditioned medium of rat fetal fibroblast (CM-RFF) was analysized using sodium dodecyl sulfatepolyacrilamidegel electrophoresis (SDS-PAGE) and biological assay on its potency to inhibit differentiation of bone marrow mesenchymal stem cells (BMSCs). Quantitative data were analyzed using statistical T-test on Minitab program. The results showed that based on the population doubling time, the proliferation rate was increased in line with the number of passages and basedon the SDP-PAGE analysis, the fibroblast cells secreted several protein included in estimation a 34 kDa protein that can be maintained until 9 passages. Addition of 25% CM-RFF into mDMEM (without FBS) did not increase the percentage of bone marrow mesenchymal stem cells-like, but signiJicantly increased the percentage of fibroblast. The presenced of LIF in the medium significantly increased the percentage of BMSCs-like and fibroblast. It can be concluded that thegrowth and protein secretion of rat fetal fibroblast cells can be maintain until 9 passages; but the secreted protein could not increased the percentage of the bone marrow mesenchymal stem cells like
Infektifitas berbagai derajat kematangan proglotida cacing pita hymenolepis diminuta (Rudolphi) pada: 1 Kutu beras tribolium castaneum (Herbst)
Get PDFStudies on the effect of the level of maturity of proglottids on the infectivity of Hymenolepis diminuta (Rudilphi) in the intermediate host Tribolium castaneum (Herbst) have been carried out in the Helminthology Laboratory, Faculty of Veterinary Medicine Bogor Agrigultural University.The experiment was carried out using 5 groups of 30 Tribolium castaneumeach which were infected with Hymenolepis diminuta prolottids of different levels of maturity. Each Tribolium group was fed 5% length of the posterior proglotids.The data obtained were analized using analysis of variance continued with Duncan test, where necessary, and analysis of reggression. The number of eggs produced by adult Hymenolepis diminuta originated from all 5 groups of 5% pasterior proglottids were positively correlated with the levels of maturity of the proglottids. The number of cysticercoids produced by the experimental Tribolium were also positively correlated with the maturity levels of the proglottids. In contrast the levels of infectivity, in percentage, of the 5 proglottid groups in the Tribolium were not significantly different from each other indicating that the infectivity of Hynlenolepis diminuta eggs in the Tribolium was not affected by the degree of maturity of the proglottid. From the result of the studies it is concluded that more or less 25% of the posterior proglottids of Hymenolepis diminuta in the rats were gravid.
Identifikasi infeksi Koi Herpes Virus (KHV) pada ikan Koi (Cyprinus carpio) dengan metode Polymerase Chain Reaction (PCR), imunositokimia dan imunohistokimia
No full textKoi herpes vivus (KHV) disease has been diagnosed in koi and goldfish, and KHV is believed to remain in the body of its host to survive so that goldfish have the potential us a carrier virus. The purpose of this test is to look for another alternative method in identzfiing a particular fish disease Koi Herpes Virus (KHV) that is more simple and practical, rapid, precise and accurate as well as the examination results are expected to match the other examination techniques. Sample testing activities are positively infected with KHV derivedfrom Blitar marked by the characteristics of the most visible of the lesions (injuries) is congestion, bleeding onjns or body, or hemorrhagic on the basis of dorsal fins, pectoral fin, and anal Jins and the operculum, necrosis and nodules (nodule) in the gills white. The result of the testing activities identijcation of KHV by PCR, IHC and ICC were by using PCR samples obtained 7 positive KHV with DNA quality between 1.83 to 1.98 and DNA quantity between 31 7.89 to 492.08 at 290 bp DNA bands. It can be concluded that the alternative examination or identification of infections of Koi Herpes Virus (KHV) in addition to the koifish using PCR, immunohistochemistry, also can be developed using immunocytochemistry method (Streptavidin Biotin) due to simpler procedures, work more practical, lower cost, faster time, precise and accurate target.
Preparasi antigen KHV untuk pencegahan infeksi KHV pada ikan koi (Cyprinus carpio)
No full textInfeksi Koi Herpes Virus merupakan penyakit sangat serius yang menyerang ikan mas dan koi. Penyakit ini menyebabkan kematian massal sebesar 80-95% dari total populasi dan menimbulkan kerugian ekonomi yang sangat besar. Tujuan dari studi ini adalah untuk mendapatkan antigen HlV yang dapat digunakan untuk mencegah infeksi KHV. Metode preparasi antigen dilalakukan dengan membuat suspensi organ 10 % dari seluruh organ target ikan yang positif terinfeksi KHV, kemudian dipekatkan sehingga menghasilkan konsentrat organ (antigen). Sebagian dari konsentrat organ (antigen) yang dihasilkan, di-inaktif-kan dengan formaldehyde 0,3%, Kemudian disuntikkan pada ikan koi sehat (vaksinasi). Penyuntikan dilakukan sebanyak 3 kali dalam kurun waktu 4 minggu. Satu minggu setelah penyuntikan ke-3 dilakukan uji tantang. uji tantang dilakukan dengan menyuntikan virus aktif KHV dengan dosis 0,1 ml secara intraperitoneal pada koi yang telah divaksin. Sebagai kontrol, dilakukan juga penyuntikan pada koi yang tidak diberi perlakuan. Hasil uji tantang menunjukkan bahwa semua ikan yang divaksinasi dengan ketiga metode preparasi antigen tidak menunjukkan gejala klinis terinvaksi KHV
Pengaruh pemberian vitamin C dan Vitamin E terhadap tantangan E. Coli Patogen pada ayam Pedaging
No full textThis study was undertaken to asses the effects of dietary vitamin C, vitamin E, vitamin C and vitamin E, combined with E. coli bacterin against patogenic E. coli challenge in broiler chickens.The completely randomized design was used, in 4 x 2 factorialexperiment i.e. consisted 4 vitamins and two bacterins. Each treatment combination had 10 chicks as replication, hence 80 broiler chicks were used in this study. Data obtained from the study were analysed using analysis of variance or Kruskal Wallis test in order to identify the influence of treatment factor and their interactions.The chickens were fed standard ration supplemented with 300 mg vitamin E or 330 mg vitamin C or 300 mg vitamin E and 330 mg vitamin C or no vitamin supplement per kg ration as control.E. coli bacterin inactivated by heat, was given to the chickens when they were 2 weeks old. The bacterin was injected directly to the airsacs of the treatment groups.At 4 weeks of age, all of the chickens were challenged with patogenic E. coli given directly to the airsacs.The data showed that the supplemental of either vitamin E, vitamin C, or vitamin E and vitamin C significantly reduced pathological lesions caused by E. coli challenge, whilst the E. colli bacterin alone did not. Suplemental vitamin C, or vitamin C and vitamin E, and also injection of E. coli bacterin significantly reduced chicken body weight
Cinta dan neurotransmiter
No full textLegenda Yunani kaya akan cerita menarik, seperti Aphrodite dewi cinta dan erotik atau eros dewi inspirasi cinta yang akan menyatukan pasangan, Ondine curse atau kutukan Ondine terhadap suaminya yang menyeleweng dan mengambil pusat otomasinya sehingga suami mati karena lupa bernafas. Narsisme yang bangga terhadap dirinya sendiri dan akhirnya mati karena ingin melihat dan mengagumi dirinya dengan berkaca di air dan akhirnya tenggelam. Semua mitologi tadi akhirnya menemukan logos atau pemikiran logis dan ilmiah. Ondine curse diterangkan dengan pusat pernafasan di otak. Bangga diri dan cinta dicoba diterangkan di otak. Bangga diri berkaitan dengan paras serotomin. Dalam perkembangan ajaran zaman Romawi yang dipengaruhi ajaran gereja muncul karya sastra Dante yang merupakan hasil renungan yang berdasarkan kepada sabda biblical tentang dosa, dan ada tujuh dosa dasar dari manusia yakni luxuria (berlebihan/extravagance) termasuk disini lust atau dorongan sexual, gula (rakus/gluttony), avaritia (serakah/greed), acedia (malas/discouragement ), ira (marah/wrath), invidia (iri/envy), superbia (bangga diri/pride). Dalam perkembangan ilmu pengetahuan kesemua dosa tadi ada di otak, yakni pada sistem saraf. Neurotransmitter atau neurohormon mempengaruhi perilaku-perilaku yang disebut dosa tadi dan dosa menjadi lebih mudah untuk diterangkan atau kita bisa mengatakan itu bukan dosaku melainkan neurotranmitter atau neurohormon yang mempengaruhi. Perkembangan ajaran agama di barat juga mengajarkan tujuh jalan kesucian, yakni rendah hati (humility), beramal (charity), murah hati (kindness), sabar (patience), kesucian (chastity), kesederhanaan (temperance), dan rajin (diligence). Jika ini merupakan kebalikan dari dosa maka kemungkinan neurotransmitter juga mempengaruhi kesucian tadi ada di otak makan bukan tidak mungkin cinta juga ada di otak dan neurotransmitter memegang peranan penting dalam proses tersebut