Hemera Zoa
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KIVP-7 Perangkat Mikrofluidik dari Katun untuk Analisa Semi-kuantitatif dalam Menunjang Diagnosa Cepat di Dunia Kedokteran Hewan
Get PDFDiagnosa cepat merupakan hal yang menjadi tuntutan dalam dunia medis saat ini. Perangkat diagnostik cepat dikembangkan guna membantu proses diagnosa segera diperoleh agar tindakan yang cepat dan tepat pada pasien dapat segera dilakukan (Castellote et al. 2003). Perangkat diagnostik cepat juga dikembangkan untuk tujuan penapisan dalam suatu populasi hewan untuk memantau kejadian suatu penyakit (Yetisen et al. 2013). Perangkat diagnosa cepat umumnya sangat sederhana, mudah digunakan tidak memerlukan peralatan khusus, harga relatif terjangkau, proses perolehan hasil langsung terbaca dalam hitungan menit (Bissonnette & Bergeron 2010). Teknologi diagnostik cepat sangat membantu tenaga medis untuk mendapatkan hasil sebelum uji lanjut melalui uji laboratorium. Uji laboratorium standar umumnya berlangsung dalam waktu yang relatif lama sehingga proses penyakit tidak dapat segera terdiagnosa dengan cepat. Proses penegakan diagnosa dalam dunia kedokteran hewan dengan jumlah spesies yang lebih banyak dari dunia medis kedokteran manusia memiliki tantangan yang lebih besar dengan keunikan tersendiri (Lewis & Klausner 2003). Tren perkembangan saat ini, perangkat diagnostik dikembangkan dengan tujuan pemantauan mandiri bagi klien dari rumah sebelum pasien dibawa ke klinik atau rumah sakit untuk ditangani oleh dokter hewan (Yager et al. 2008). Tulisan ini membahas sekilas tentang perangkat diagnostika cepat berbasis mikrofluida (mikrofluidik) yang dapat dimanfaatkan dalam dunia kedokteran hewan guna menunjang percepatan proses penegakan diagnosa
PCS-7 Lead Detection in Blood and Liver of Cattle sold in the Traditional Market of Denpasar City
Get PDFLead contamination in many foods is reported to endanger consumers\u27 health. Beef as one of the food of animal origin can be contaminated by lead mainly due to the maintenance of cattle in contaminated areas. Balinese cattle that are kept in urban landfills are reported to be contaminated with high levels of lead in the blood and in other tissues with lower levels [4]. The threat of human health from eating foods that contain leads can cause anemia because leads can substitute iron in hemoglobin [2]. Lead poisoning can also cause malfunction of various organs such as liver, kidney [1], lungs, spleen [7] and brain [9] in the form of cognitive impairment [5]. The high threat due to contamination of leads to health, then the meat health check should also be made on the presence of leads. One of the inspection efforts on the presence of lead contamination in beef, then the place of sale in the traditional market is a good location for sampling examination
AEVI-22 Investigasi Outbreak Penyakit Jembrana di Kabupaten Padang Lawas, Provinsi Sumatera Utara Tahun 2017
Get PDFPenyakit Jembrana (Jembrana Disease/JD) merupakan salah satu penyakit akut dan fatal pada sapi Bali. Sejauh ini Penyakit Jembrana (JD) hanya terkenal di Indonesia dan hanya menyerang sapi Bali. Informasi terakhir dilaporkan adanya kasus penyakit Jembrana di Provinsi yang berbatasan langsung dengan Sumatera Utara yaitu di Provinsi Riau dan Sumatera Barat. Situasi ini meningkatkan risiko penularan penyakit Jembrana Ke Sumatera Utara terutama pada Kabupaten yang memiliki populasi sapi Bali dan berbatasan langsung dengan kedua provinsi tersebut. Kasus dugaan penyakit Jembrana Tahun 2017 pertama kali dilaporkan dari Kabupaten Padang Lawas pada tanggal 12 Januari 2017. Dalam laporan tersebut disampaikan meluasnya kasus penyakit pada sapi Bali dan telah menyebabkan kematian pada 18 ekor sapi Bali khususnya di kecamatan Huta Raja Tinggi. Kasus tersebut kemudian ditindaklanjuti dengan pelaksanan investigasi lapangan dan pengujian laboratorium oleh Balai Veteriner Medan. Investigasi yang dilaksanakan bertujuan untuk mengetahui sejarah kejadian penyakit, mengetahui penyebab dan atau faktor risiko kasus penyakit tersebut, sehingga dapat diberikan rekomendasi yang sesuai
PCS-12 Complex Oligosaccharide Detected in the Lingua of Hystrix javanica by Lectin Histochemistry
Get PDFHystrix javanica or usually called Sunda porcupine is wildlife animal that only found in Java, Bali, Sumbawa, Flores, Lombok, Madura, and Tonahdjampea (Indonesia) [1]. International Union for Conservation of Nature and Natural Resources (IUCN) has classified this Rodentia into Least Concern criteria, that means the species is relatively widespread and abundant [2]. Nevertheless, their population in nature was threatened, since this mammal was considered as pest by some people.There were anatomical data of Sunda porcupine organ, but it still limited. One of the ways to determine the detail function of the organ can be done by making histological preparations. Lectin histochemistry has been used widely as a probe to detect sugar residues in the organs or tissues because lectin can bind specifically to carbohydrate residues in term of glycoconjugates [3]. Analysis the composition or type of sugar residues in the organs can help to understanding the role of their function.The aims of this study were to detect and find out the distribution of complex oligosaccharide in the lingua of Hystrix javanica using lectin histochemistry method. This data will completes the information that has been obtained before about mucopolysaccharide type of major salivary glands of Hystrix javanica [4]
JSPS-7 Bovine Respiratory Syncytial Virus Infection Enhances Pasteurella multocida Adherence on Respiratory Epithelial Cells
Get PDFBovine respiratory syncytial virus (BRSV) is a single negative-stranded RNA virus belonging to the Paramyxoviridae family and shows a close genetic relationship with human respiratory syncytial virus (HRSV). BRSV is the primary etiological agent of respiratory disease in calves aged up to 12 months [1]; beef and dairy cattle worldwide [2, 3]. Initial infection by BRSV alter bovine immune system and facilitates secondary infection of the lower respiratory tract by bacteria [1, 4]. Therefore, BRSV is considered to be a causative agent of bovine respiratory disease complex, which results in economic losses to farmers because of the morbidity and mortality in cattle [2, 3]. According to our preliminary findings based on the gene detection from respiratory samples, paired virus and bacteria were detected; Pasteurella multocida (PM) was the most common bacterial agent (unpublished data). PM is common in the nasopharynx of cattle [5, 6], although PM appears to be part of the normal flora, it can contribute to pneumonia when cattle stressed and/or infected by a respiratory virus [7]. However, the interactions between multiple agents associated with BRDC are not clear. Therefore, the aim of this study was to investigate the effect of BRSV infection on PM adherence to respiratory epithelial cells
FA-2 Gastrointestinal Diseases of Pigs: Diarrhoea Pre-Weaned Pig in Breeder Area
Get PDFAll phases of pig raising are important, from the time of the womb, the prestarter, the starter, the grower, the finisher, the sires (sow), and the males (boar), all related to each other to produce maximum performance. However, the initial phase of pig breeding is indeed a phase most important.In this phase, the state of the digestive tract is still relatively sterile from microorganisms, does not have a good body temperature regulation system, immune system is not functioning well so vulnerable to the presence of infectious diseases from outside in the initial phase, piglets tend to be environmentally sensitive and easy to stress.The symptoms of diarrhoea (scours) at the time of pre-wean, weaning or change of feed is necessary to watch out for. In theory, diarrhea is a disease of gastrointestinal disease (enteritis) that attacks many piglets or young pigs, caused by several factors:*Non-infections, such as sudden weaning stress, weather changes (from summer to rainy season or vice versa), cage displacement, density, poor cage sanitation, starving and sudden change of feed in pigs to be weaned.*Infection, such as bacterial (colibacillosis), viral (coronavirus), or parasitic disease (coccidiosis).At the time of weaning, piglet has a high risk of stress because it is psychologically separate from the mother. Stress increases as pigs also have to adapt to the environment and the type of feed from the normally wet becomes more complex and dry.In addition, in the digestive tract, the piglet has changed the size of the surface of the small intestine villi to be shorter so that it affects the digestibility and absorption capacity of the feed. Another consequence is the presence of changes in the immune system and intestinal microflora so that if there is a beneficial bacterial imbalance, can cause the occurrence of diarrhea
MP-5 Antibiotic Resistance of Klebsiella Species Isolated from Broiler Chickens in Sukabumi and Bogor Areas
Get PDFAnimal protein needs in Indonesia increase every year. One source of animal protein that is affordable for all levels of society is poultry. Poultry mostly farmed in several parts of Indonesia. The region that has the largest poultry population in Indonesia is the province of West Java. The regions in West Java that contribute the highest poultry populations are Sukabumi and Bogor. The largest number of poultry populations is broiler chicken population, which is 108,304,978 tails.Antibiotics often used in livestock and poultry as a prevention of disease transmission and as a growth promoter. Routine use of antibiotics in the livestock industry, especially chickens, has a negative impact. One of them is the emergence of antibiotic resistance. One of the bacteria that has experienced antibiotic resistance is the Klebsiella species.Klebsiella bacteria are Gram-negative bacteria are normal flora in the oral cavity, skin and intestines, but can become pathogens in animals and humans under certain conditions. This bacterium is one of the causes of bacterial diseases that attack large livestock and poultry. Many reports say that Klebsiella species have experienced resistance to antibiotics. Klebsiella had experienced resistance to several antibiotics, namely ampicillin as much as 66.7%, Nalidixic Acid 61.8%, tetracycline 59.8% and trimethropin 50%. Klebsiella bacteria that are resistant to antibiotics are very dangerous for animal and human health. Diseases caused by Klebsiella bacteria that are resistant to antibiotics will be difficult to cure. Akova (2016) states that Gram-negative and Gram-positive bacteria that experience resistance will produce infections that are difficult to treat or cannot even be treated using antimicrobials
MP-7 Isolation of Antibiotic Resistance Gene from Staphylococcus aureus from Chicken Poultry Farm in Bogor West Java Indonesia
Get PDFStaphylococcus aureus is an opportunistic pathogen in human and another animal species. It also associated with staphylococcosis, osteomyelitis, arthritis, and bumble foot disease in poultry. Antibiotics including penicillin, erythromycin and tetracycline are widely used for treating infection caused by S. aureus.Chicken poultry play important role in food supply as one of protein source in Indonesia. West Java province has largest population of chicken poultry, where Bogor has the largest population of chicken poultry with 19.75.850 chicken in 2018. In order to maintain the production, farmers used antibiotics for prophylaxis, growth promotor, and therapy. However, extensive use of antibiotic can be found in low income and developed country, caused the increasing number of antibiotic resistant bacteria.The aim of this study is to determine the antibiotic resistance pattern of S. aureus isolated from cloacal swab of chicken poultry in Bogor West Java Indonesia
MP-16 Characterization of Avibacterium paragallinarum Caused Infectious coryza/Snot: Satellite Colony Phenomenon
Get PDFInfectious coryza (IC) is an acute upper respiratory disease of poultry that can appear in all ages. Some of clinical signs that are commonly seen in IC are rhinitis, facial swelling or edema, lacrimation, anorexia, and retarded growth in young poultry [1.2.3]. The disease can be found worldwide, especially in tropical countries [4]. Infectious coryza is very important in the chicken farm industry in developed and developing countries, including Indonesia [5]. The large economic losses due to IC such as increased number of culling, decreased egg production (10-40%), decreased body weight, stunting growth, and some mortality (2-10%) [4].Avibacterium paragallinarum which was previously classified as Haemophilus paragallinarum is a causative agent of infectious coryza in laying and broiler chickens, quail, pearl chicken, turkey, and peacocks [4,6,7,8]. The bacteria is commensal in the mucous membrane of the upper respiratory system, is sensitive to preservation and does not last long outside the host body [8]. Factors X and V are needed for the growth of several types of A. paragallinarum. According to in vitro growth requirements, A. paragallinarum can be either nicotinamide adenine dinucleotide (NAD) independent or NAD-dependent. The reduced form of NAD (NADH; 1.56-25 µg/ml) and the oxidized form (20-100 µg/ml) is required for in vitro growth in most isolates A. paragallinarum that show satellitic colony on a medium [9]. The description of the need for V factor of field isolates A. paragalinarum has been few reported. The aim of this research is to find out the phenomenon of satellite colonies from a variety of poultry isolates