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EFFECT OF LIGHT INTENSITY ON THE CELL DENSITY, DIAMETER AND CELL VOLUME ZOOXANTHELLAE FROM ISOLATE SOFTCORAL Zoanthus sp
Get PDFCoral reefs are one of the aquatic ecosystem trophic productive for aquatic organisms. Coral reefs can not be separated from the various threat of causing damage. Physiological damage that is visible coral bleaching. Bleaching of coral reefs due to causes it may be the biggest threat to coral ecosystems due to widespread in various regions. Coral bleaching causing the loss of endosymbiont coral or reduction of photosynthetic pigments zooxanthellae of corals. Zooxanthellae need light for photosynthesis. The process will produce energy which serves for the biosynthesis cell, growth and cell division. Therefore light plays an important role in the process of photosynthesis. This research aims were to know the cell density, diameter and volume zooxanthellae cells on different light intensity. The research was conducted on July-August 2016, in the Aquaculture Laboratory, Program Study of Aquaculture Faculty of Agriculture University of Lampung. Observed parameters were the density, diameter and volume zooxanthellae cells. Research used the 4 treatments and 5 replications namely 3800 lux (IC1), 6250 lux (IC2), 7980 lux (IC3), and 11800 lux (IC4). Measurement of the density and diameter of zooxanthellae cells used a hemocytometer neubauer improved and objective micrometer (0.01 mm). Data were analyzed by analysis of variance (ANOVA) and followed by LSD test. The results showed that the intensity of light significantly affected the density, diameter and volume zooxanthellae cells. Intensity light given by zooxanthellae responded with increased density and a decrease the diameter and volume zooxanthellae cells in the early stages of culture (0-18 hours)
Evaluasi Kualitas Sedimen Beberapa Tambak Udang Di Kabupaten Tulang Bawang Provinsi Lampung
Get PDFPond bottom management play an important role in the success of shrimp culture. Study on the use of pond bottom pond as an indicator of shrimp pond productivity is still limitted. Shrimp culture has been developing in tulang Bawang Regency, Province of Lampung since early 1990. The waste produced by shrimp culture activity is possible to reduce pond bottom soil quality due to high organic compund and toxic material. The aim of this research was to evaluate the shrimp ponds bottom condition in Tulang Bawang Regency, Province of Lampung. This research was an explorative one. Data collection was done towards 12 shrimp pond units during water preparation period (pre-spreading) with the different locations of each pond from the watergate. The collected data of pond soil qualities were organic matter content, chlorophyll a, cation exchange capacity, oxidation-reduction potential, pH, and soil texture. The research results showed that soil qualities in shrimp ponds in Tulang Bawang Regency were still in range for shrimp culture. In generally, shrimp ponds in the area have soil textute of clay, sandy, and sandy clay.Chlorophyll a content, oxidation reduction potential (ORP) and organic carbon matter content were 21,5 μg/g, 76 mv and 1,26% in average respectively. Meanwhile cation exchange capacity and soil pH were 11,2 me/100g and 6,8 in average
Keanekaragaman Dan Kepadatan Gastropoda Di Perairan Desa Morindino Kecamatan Kambowa Kabupaten Buton Utara
Get PDFGastropods have important economic value because the shell can be used for a variety of decorations are expensive and the meat as a source of food. Gastropods also a key organism in the food chain in aquatic ecosystems. This research aims to determine the diversity and density of gastropods and environmental conditions that affect gastropods. The usefulness of the research is as information for the relevant agencies and parties that need as well as information for further research. The research was conducted in morindino village watersfrom August to October 2014. The placement transect squared/plots based on the state of the substrate is sandy and rocky beaches. The results found 20 species of gastropods which belong to 17 genera in 16 families. Density on the rocky shore organisms higher than the sandy beach that is 29.11 ind/m2 while the sandy beach that is 1.44 ind/m2. Rocky shore species diversity in higher at 1.8742 while the lower sandy beach which is 1.5858. However overall, the diversity of marine gastropods in Morindino village water was included in the medium category. Water velocity Parameter affects the density and diversity of gastropods
Hubungan Perubahan Kualitas Air Dan Pertumbuhan Fitoplankton Berbahaya Pada Lingkungan Budidaya Ikan Di Perairan Ringgung Teluk Lampung
Get PDFWater quality becomes the critical factor of successfully aqua culture process; especially this is in aquaculture of floating cages. Otherwise, unstable water quality could impact the negative effect toward aquatic ecosystem such as the large explosion of harmful phytoplankton population (HABs). The objective of this research is to analyze a relation between the water quality of floating cages and the growth of harmful phytoplankton population by using canocial corelation analysis. Water quality research includes its brightness, the deepness, disolved oxygen, salinity, pH, value of NO2, NO3, NH3, and PO4 and toxic phytoplankton obtained during the study also indicate the amount of phytoplankton growth The results were obtained 14 species of toxic phytoplankton in the study sites. Overall abundance of phytoplankton dominated by Choclodinium, Trichodesmium Erythraeum, Nitzschia Lanceolata dan Pseudo Nitzschia, where Choclodinium an abundance of phytoplankton with the highest density of 63 739 cells/l. Canonical correlation analysis on triplot diagram showed water quality includes : brightness, disolved oxygen, pH, NO2, NO3 and PO4 together to give effect to toxic phytoplankton abundance. Canonical correlation analysis also specifically indicate the abundance Choclodinium and Trichodesmium Erythraeum in floating nets of Ringgung Marines predominantly influenced by brightness, pH, value of NO3, and PO4
REDUCTION OF TOTAL ORGANIC CARBON AND CORRELATION WITHIN ABUNDANCE AND DIVERSITY OF PHYTOPLANKTON IN AQUAPONICS SYSTEM
Get PDFWater quality plays an important role in aquaponics system. Water chemistry parameter that may affect water quality is total organic carbon. Sources of carbon in the aquaponics system mainly derived from the decomposition of organic matter which derived from artificial feed and plant respiration. The research was conducted to study the reduction of total organic carbon concentration in the aquaponic system and the relation of abundance and diversity of phytoplankton. This research using different amount of ipomoea (Ipomoea aquatica) consist of 10, 20, and 30 rods and control treatments which use without ipomoea. The results showed that all of ipomoea treatments can reduce the amount of total organic carbon concentration in the aquaponics system. This research showed that nutrients cycle in the aquaponics system, especially total organic carbon dissolved in water is influenced by many factors such as plants, fish and primary productivity which determined by the plankton. Furthermore, a positive correlation occurred between total organic carbon concentration of the abundance and diversity of phytoplanktons (r = 0.5925; r = 0.6364). These results can be used for preliminary study of nutrient cycle model in aquaponic system
POTENTIAL IMMUNOGENICITY OF BACTERIA Aeromonas hydrophila GPL-05 AND GL-02 STRAINS AS A CANDIDATE VACCINES
Get PDFAeromonas hydrophila is very potential to be used in vaccines and disease control MAS (Motile Aeromonas Septicemia) caused by the bacteria. However, strains of A. hydrophila bacteria which have high immunogenicity limited. Not to mention, in the field often decrease the pathogenicity of the bacteria as vaccine material resulting in a decrease in the immunogenicity of a vaccine made. Therefore, it is necessary to inventory and collecting strains of A. hydrophila bacteria potentially good that vaccination continuity can be maintained. This study used an experimental method with a completely randomized design (CRD), with treatments such as P1: A. hydrophila vaccine strain GPL-05; P2: A. hydrophila vaccine strain GL-02; and P3: control (PBS), with five replicates.This study aims to assess the potential immunogenicity A.hydrophila bacterial strains as vaccine candidates. Test fish used is African catfish was about 2 months with a length of 10-15 cm and width 15-20 g. The main parameters are observed antibody titer and cross-reaction test, while supporting parameters are parameters of water quality include water temperature, pH, dissolved oxygen. Antibody titer data was analyzed by Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) at the test level of 5%, while the data and the results of cross-reaction of water quality parameters observed descriptively. The results showed that the bacteria A. hydrophila strain GPL-05 and GL-02 has a high immunogenicity, thus recommended a candidate vaccine
Penerapan Manajemen Kesehatan Panti Benih Udang Di Kalianda Lampung Selatan
Get PDFShrimp culture drives the national economy that are included in the program of industrialization of fisheries in the economic development programs of blue ( blue economy ). Shrimp farming in Lampung province remains a mainstay of the region as a foreign exchange earner . Kalianda , South Lampung is the center hatchery tiger shrimp (Penaeus monodon) and vannamei shrimp (Litopeneaeus vannamei ) the largest in Lampung. research conducted to observe the shrimp health management applied by growers of black tiger shrimp and vannamei shrimp in South Lampung and learn relationship between seed health management with quality seeds and tiger prawn shrimp vannamei shrimp health management dihasilkan. Data obtained by interview and the quality of shrimp seed obtained from observations in the laboratory with the observed light response, swimming activity, abnormality, gut contents, lipid droplets, the condition of the hepatopancreas and pigmentation. There are 22 hatchery were observed with the application of the value of health management in the high category for the third hatchery , hatchery medium category 6 and category bneih low at 13 homes. The quality of virus-free shrimp WSSV and IHHNV and the amount of bacteria in the body are normal and healthy shrimp was observed in the overall nursing bneih diamati. Relation between hatchery management application health and seed quality is reflected by the equation Y = 0,019X2 - 1,358X + 52.49 with the coefficient of determination ( R2 ) of 0.419. This relationship indicates that the quality of shrimp seed is determined by the health of hatchery management practices (r = 0.647). This study reinforces the importance of implementing health management hatchery known by farmers with biosecurity as a form of sustainable shrimp farming
Analisis Daya Dukung Perairan Puhawang untuk Kegiatan Budidaya Sistem Karamba Jaring Apung
Get PDFPuhawang Island have a potency to developed for aquaculture especially cage system. Aquaculture with cage system will be an optimum production if the environment still support for fish to growth. Environment faktor that support the growth of fish and production of cage system called carrying capacity. The aim of this research was to determined the ability of waters around Puhawang Island to support cage system especially for cultivated grouper with floating net cages. This research was conducted using descriptive method in August to September 2014. The result shown that water quality in Puhawang Island still suitable for cage system. Holding capacity could be optimized to 53.550 tons ( with carrying density is 107.100.000 of fish). This production could be reach if used 39.600 units with 6x6x3m in volume dimension, and maksimum density is 25 fish each unit cag
ASSAY OF Acinetobacter AS COMPETITOR TO VIBRIOSIS LUMINESCENCE BACTERIA IN PACIFIC PACIFIC WHITE SHRIMP(Litopenaeus vannamei)
Get PDFPacific white shrimpculture is having problems caused by bacteria infection. The cause of bacteria infection is V. harveyi, it can decrease seed production. Pathogenicity of vibriosis can decrease with inhibition by other types of bacteria. The aim of the research was to get competitor V.harveyi isolates through identification and challenge test in vitro and in vivo. The result of screening of bacterial isolates were obtained from cultivation supplement products to produced 4 pure isolates with different morphologically colonies and cells. There was one potential isolate could inhibits V. harveyi by inhibition test in vitro. Biochemical test from three different laboratories were identified that Acinetobacter sp. as competitor to V. harveyi and it was not pathogenic to Pacific white shrimp. Challenge test Acinetobactersp. against V. harveyi on Pacific white shrimp larvae was conducted through three treatments, without given V.harveyi and Acinetobacter sp. As controls; treatment with given bacteria V.harveyi and treatment with given V. harveyi and Acinetobacter sp. The challenge test results showed that treatment with given of V.harveyi and Acinetobacter sp. may decreased V. harveyi density of 6.3x106CFU/mL to 7.5x103CFU/mL which could suspected to decrease pathogenicity of competition number of colonies
PERFOMANCE GROWTH AND SURVIVAL RATE OF CATFISH HATCHED LARVAEA IN MASS CULTURED WITH THE PROVISION OF FEED Tubifex sp USING FERMENTED INDUSTRIAL DOMESTIC WASTE
Get PDFTubifex sp. merupakan salah satu pakan alami terbaik untuk larva lele. Kultur massal Tubifex sp .perlu dilakukan untuk mencukupi kebutuhan pakan lele secara kuantitas maupun kualitas khususnya pada stadia larva. Tujuan dari penelitian adalah mengkaji performa pertumbuhan dan kelulushidupan larva lele dengan pemberian pakan Tubifex sp. yang dikultur massal menggunakan fermentasi limbah industri rumah tangga. Hewan uji yang digunakan adalah larva ikan lele (C. gariepenus) dengan berat0,06±0,03 g/individu. Penelitian ini dilakukan dengan metode eksperimental menggunakan rancangan acak lengkap (RAL) dengan 3 kali ulangan yaitu perlakuan A (50 g/L kotoran ayam; 100g/Lroti afkir; 50 g/L ampas tahu); B (50 g/L kotoran ayam; 100g/L bekatul; 50 g/L ampas tahu) dan C (50 g/L kotoran ayam; 100g/L bungkil kelapa; 50 g/L ampas tahu). Pemberian Tubifex sp. sebagai pakan alami sebanyak 5 kali sehari secara ad libitum.Hasil penelitian menunjukkan bahwa terjadi pengaruh yang nyata (P<0,01) pada laju pertumbuhan dan tidak berpengaruh yang nyata (P>0,01) kelulushidupan larva ikan lele(C. gariepenus). Laju pertumbuhan relatif larva ikan lele (C. gariepenus) memiliki nilai rerata berkisar antara 17.1%-18.7% dengan tingkat kelulushidupan berkisar antara 96,8% - 98,8%. Berdasarkan pada hasil penelitian, maka perlakuan A dengan nilai RGR dan SR 18.7% dan 98.8% merupakan perlakuan terbaik untuk meningkatkan laju pertumbuhan dan kelulushidupan larva ikan lele (C. gariepenus)