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IDENTIFIKASI BAKTERI DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK BAKTERI ENDOFIT YANG DIISOLASI DARI ALGA MERAH (Gracilaria sp.)
No full textGlacilaria sp. is one type of red algae that is widely found in Indonesia. Red algae has anti-microbial, anti-bacterial, anti-viral, anti-fungal, and other benefits. Endophytic microbes associated with seaweeds such as bacteria and fungus are thought to be able to produce the same bioactive compounds as their host, seaweeds. Several studies have also shown that the antibacterial activity of endophytic microbes can inhibit pathogenic bacteria. This study was conducted to identify endophytic bacteria present in Glacilaria sp. in inhibiting the growth of pathogenic bacteria (Vibrio sp. and S. aureus). Endophytic bacteria found in Gracilaria sp. isolated and tested for antibacterial activity using Staphylococcus aureus and Vibrio sp. bacteria. with disc disc method. the results showed that endophytic bacteria of the red algae Gracilaria sp. has antibacterial activity against bacteria S. aureus and Vibrio sp. It has inhibitory zones of 8 mm and 7.6 mm, respectivel
HUBUNGAN EKSPRESI miRNA-21 DENGAN TARGET PDCD4 DAN miRNA-143 DENGAN TARGET BLC-2 PADA KASUS KANKER SERVIKS
Get PDFA study has been carried out to compare the expression of miRNA-21 and miRNA-143 with target mRNA PDCD4 and BCL-2 in cases of early-stage cervical cancer compared to advanced stages. These data can be used as information for the development of non-invasive cervical cancer prognostic methods. The study was conducted by taking serum samples and exfoliativecell samples from normal subjects and subjects with cervical cancer detection and then analyzed using qRT-PCR. Samples of cervical cancer patients consisting of 15 subjects and 4 normal subjects were used to obtain relative quantity values for the expression of miRNA-21, miRNA-143, mRNA PDCD4 and BCL-2. In the study of miRNA-21, there was no significant difference in determining cervical cancer with p>0.05 using the t-test (0.594) and a significant relationship was found between miRNA 21 and PDCD4 with a strong correlation (p<0.05, r = -0.563; Pearson). The relationship between miRNA-143 and the Bcl-2 target gene in this study showed a weak and insignificant correlation (r = -0.101; p > 0.05; Pearson). There was a significant relationship between miRNA-21 and PDCD4 in early-stage and advanced-stage samples, while the relationship between miRNA-143 and the Bcl-2 mRNA target gene showed no significant correlation
Aktivitas Antibakteri Minyak Atsiri Kulit Jeruk Limau (Citrus amblycarpa (Hassk.) Ochse) Dalam Mengendalikan Bakteri Streptococcus mutans: Antibacterial Activity of Citrus Limau Atsir Oil (Citrus amblycarpa (Hassk.) Ochse) in Controlling Streptococcus mutans
Get PDFDental caries is an infection disease of tooth tissue caused by Streptococcus mutans bacteria. Atsiri is essential oil is a liquid-shaped compound that is thought to have potential as an antibacterial. The purpose of this study was to determine the antibacterial potential of essential oil of limau lime in controlling Streptococcus mutans bacteria. The antibacterial test in this study used disk disfussion method with 8 treatment groups and 4 replications is 5 concentrations of atsiri oils, 1 positif disc amoxicillin antibiotic, 2 negative control with DMSO and aquades. The data obtained were analyzed with normality test, Anova test and BNT test. Isolation of atsiri’s oil of limau lime in this study using the method of steam-water distillation.
Streptococcus mutans bacteria were isolated from dental caries patients and tested using macroscopic, microscopic, gram staining, and biochemical methods. The study found that the atsiri oils of limau lime has antibacterial activity in vitro against Streptococcus mutans bacteria, as evidenced by the formation of an inhibit zone. Concentration as 100% with 15.38mm inhibitory zone diameter is the most effective concentration to inhibit the growth of Streptococcus mutans bacteria. The statistical analysis revealed a significant influence between the treatments at the 5% significance level
ANALISIS MUTU ORGANOLEPTIK DAN UJI Salmonella sp. PADA IKAN CAKALANG ASAP DI PASAR REMU, KOTA SORONG
Get PDFSmoked skipjack is a type of product that is sold in many markets in Indonesia, including at Remu Market, Sorong City. However, the quality and safety of smoked skipjack tuna is a matter that needs to be considered by the public as consumers. The research method used was a descriptive study by observing the organoleptic quality of smoked skipjack tuna and detection on Salmonella sp..The results of this study showed that the organoleptic quality value of the appearance of the P sample of smoked skipjack tuna at the Remu market, Sorong city had the highest value of 8.87, then the highest odor test was 8.73, the taste value was 8.93, and the value of the texture was 8.40. However, the value of mushrooms and slime from the three samples showed the same value (9.0) which indicates that there was no fungus and slime in the smoked skipjack tuna observed. The results of the detection test Salmonella sp. showed that the P sample was safe from contamination by Salmonella sp. compared to sample R and sample Q
PENGARUH BEBERAPA TIPE PENGENDALIAN GULMA PADA PERUBAHAN STRUKTUR VEGETASI TUMBUHAN BAWAH DI PERKEBUNAN JABON (Neolamarckia cadamba (Roxb.) Bosser.)
Get PDFJabon (Neolamarckia cadamba (Roxb.) Boser.) is commonly planted for many purposes, such as furniture, raw material for industries, and land rehabilitations. Eventhough this tree has no significant pests and diseases, weed control treatments are required. Many weed control treatments are known to affect vegetation structure of weed. Thus, the objective of this study was to examine the effect of weed control treatments to the vegetation structure of weed under Jabon plantation. This study was conducted in Kecamatan Tanah Putih Tanjung Melawan, Kabupaten Rokan Hilir, Riau Province in 2012. Non Factorial Randomized Group Design with five replications was applied to perform the study. There were four treatments (glyphosate, paraquat, mixture of glyphosate and paraquat, and legume cover crop/LCC) and control. The results revealed that glyphosate and paraquat had the highest value in surpressing the weed growth as much as 34,45% and 31,11% (P>0.05) after one month application. Yet, after three months, LCC was significantly surpressed the number of weed (49,44%) (P<0.05) compared to other treatments. Among all weed species, Blumea sp. was the dominant species after one month of glyphosate and paraquat application. Nevertheless, application of glyphosate-paraquat mixture and LCC resulted Ottochloa nodosa as the dominant species after one month application
EKSPLORASI JENIS AMFIBI DI SEPANJANG PINGGIRAN DANAU SIPIN JAMBI, SUMATERA TENGAH
Get PDFThis study aimed to determine the diversity of amphibian species in different habitat types along shore of Lake Sipin. This study used a combination of path (Transect) and VES (Visual Encounter Survey) methods. Research area was determined using purposive sampling based on the function of the habitat and the number of prey animals. The population in this study was all amphibians in the Sipin Lake. The samples were all amphibians caught using fishing nets or bare hands. Identification of amphibian species found in the lake used field guidebooks and online-based guide. The results of research carried out along the edge of Lake Sipin showed 215 individuals, 8 species and 4 families were found to be amphibians. The diversity index for amphibian species generally shows an H' index with an H' value of 1.19, which is included in the medium diversity index value. The overall amphibian species evenness index value is classified as an unstable community (E=0.573) and the amphibian species richness index value generally shows a low species richness index (Dmg=1.303). Meanwhile, the value of the community similarity index or Index of Similarity (IS) generally shows that natural habitats and tourism are relatively high with an index value of 90%
DISTRIBUSI KAWASAN MANGROVE DI PULAU POTO KABUPATEN BINTAN MELALUI PENGINDERAAN JAUH
Get PDFMangrove is a unique vegetation type growing along the coastline and estuarine in tropical and subtropical countries. Mangroves in Indonesia are distributed in all major islands and small islands from Aceh on westside stretched to Papua on the east. This study is to monitor the mangrove area in Poto island a small island part of Kabupaten Bintan, Province of Riau Kepulauan. The difference in spatial resolution of remote sensing data Sentinel 2A and Pleiades were used to classify the land use including the mangrove area. The supervised classification by maximum likelihood classification showed the high-resolution image using Pleiades has higher accuracy for mangroves 81.99% and overall accuracy 90% (kappa 0.86) compared to Sentinel 2A for mangrove 72,34% and overall accuracy 80% (kappa 0.73). The mangrove area approximately 257 ha is strip-lining along the coastline with the largest mangrove area located on the southside of the island. Further research is to identify the mangroves’ formation and the serial condition of mangroves on the different times
KONSTRUKSI POHON FILOGENETIK SECARA IN-SILICO JAMUR BERKERABAT DEKAT Trametes versicolor SEBAGAI TERAPI LUPUS BERDASARKAN MARKER 18s rRNA
Get PDFABSTRAK
Pengobatan herbal telah banyak dikembangkan contohnya adalah pengembangan obat tradisional pada penyakit Lupus. Diketahui bahwa Jamur Tremetes versicolor adalah jamur yang memiliki sifat immunodulator yang berfungsi untuk mengobati penyakit autoimun yaitu Systemic Lupus Erythematosus (SLE) yang memiliki senyawa protein unik Polysaccharide Peptide Krestin (PSK). Tetapi jamur Trametes tidak banyak dikonsumsi oleh masyarakat Indonesia. Tujuan dari penelitian ini adalah mengetahui jamur yang berkerabat dekat dengan Trametes versicolor yang dapat juga dijadikan sebagai terapi pada penyakit autoimun dan dapat dikonsumsi di Indonesia. Studi yang digunakan adalah studi filogenetik secara in-silico dengan jamur yang berkerabat dengan jamur Trametes versicolor dengan penanda gen 18s rRNA yang diperoleh dari genbank NCBI. Data molekuler sekuen menunjukkan bahwa jamur yang berkerabat dekat dengan jamur Trametes versicolor adalah Ganoderma lucidum, Lenzites betulinus, Agaricus bisporus, Agaricus subrufescens, Auricularia polytrica, dan Auricularia auricula-judae berdasarkan penanda gen 18s rRNA. Jamur ini dapat dijadikan sebagai obat alternatif untuk terapi penyakit autoimun dan mendapatkan senyawa yang terkandung dalam jamur tersebut dengan studi in-vitro lebih lanjut.
Kata Kunci: In-Silico, Trametes, Ganoderma, Lenzites, Auricularia, Agaricus, Autoimun
ABSTRACT
Development of herbal treatment have been done quite frequently, for example the development of traditional medicines for Lupus. It is known that Tremetes versicolor mushroom is a fungus that has immunodulatory properties which functions to treat autoimmune disease namely Systemic Lupus Erythematosus (SLE) which has a unique protein compound Polysaccharide Peptide Krestine (PSK). However, Trametes are not widely consumed by Indonesian people. The purpose of this study was to find out which fungi are closely related to Trametes versicolor which can also be used as a therapy for autoimmune diseases and can be available for consupmtion in Indonesia. This study used an in-sillico phylogenetic with fungi that are related to Trametes versicolor mushroom with the 18s rRNA gene marker obtained from the NCBI genbank. Sequential molecular data shows that fungi closely related to Trametes versicolor mushrooms are Ganoderma lucidum, Lenzites betulinus, Agaricus bisporus, Agaricus subrufescens, Auricularia polytrica, and Auricularia auricula-judae based on the 18s rRNA gene marker. This fungi can be used as an alternative medicine for autoimmune diseases treatment by obtaining compounds contained in these fungi with further in-vitro studies.
Keywords: In-Silico, Trametes, Ganoderma, Lenzites, Auricularia, Agaricus, Autoimu
KEANEKARAGAMAN FAUNA DI TAMAN KEANEKARAGAMAN HAYATI CILEGON PT. CHANDRA ASRI
Get PDFThe establishment of Taman Kehati Asri by PT Chandra Asri is a form of protection of biodiversity to improve the quality of biodiversity, including fauna. The existence of fauna is very important in ecosystem activities because fauna is a consumer in the food pyramid. If a population decreases, it will have a negative impact on the continuity of food webs and hinder the flow of energy cycle. This study aimed to identify the diversity of fauna in the Taman Kehati Asri area of PT. Chandra Asri Petrochemical, in Gunung Sugih Village, Ciwandan District, Cilegon City, Banten. The research was conducted in October 2022. The methods used were point count and cruise method. Based on the research results, it was found that there were 37 species of fauna belonging to 4 classes, namely birds, mammals, herpetofauna and insects. The result of total species diversity index was 2.98 which was categorized as in the medium category. Furthermore, the fauna dominance index was 0.075 which indicated that one or several species of fauna were not dominated in that area
DESKRIPSI SEMUT PADA HABITAT TERTUTUP DAN TERBUKA DI KAWASAN HUTAN KOTA ARBORETUM CIBUBUR JAKARTA TIMUR
Get PDFUrban forest is a special area in anurban planning that plays a role in preserving the environment, as a recreation area and as one of the lungs of the city which helps in maintaining the health of city air. The existence of ants in the urban forest plays a role in maintaining the stability of the urban forest ecosystem which generally has both open and closed habitats. The purpose was to provide information regarding the description of ant species in open and closed habitats in the urban forest of the Cibubur Arboretum, East Jakarta. This research was conducted in the Cibubur Arboretum City Forest, East Jakarta. Sampling was carried out from 5─12 December 2022 using a pitfall trap which was placed in each habitat with a distance between traps of 5 m. As many as 14 ant species are tramp ants which have high adaptability to environmental disturbances. Of the 14 species found, 12 species were not found in closed habitats, namely, Camponotus arrogans and Polycharis becarrii. Pheidole parva was found in open habitats, meanwhile, in closed habitats, Odontoponera denticulata was found in the highest number