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PENGARUH SUHU TERHADAP DIFERENSIAL LEUKOSIT SERTA KADAR MALONDIALDEHIDE (MDA) BURUNG PUYUH (Coturnix coturnix Japonica)
Get PDFThe main problem in tropical poultry farming that often happens is a high mortality rate. Deaths occurrance mostly by heat stress, a condition which is caused by high temperature ( > 28 ° C). Prolonged heat stress was given to 24 quails grouped in four groups: A (39 ° C), B (41 ° C), C (43 ° C) and D (45 ° C). Provision of heat stress was done when the quails were 2 days old. Blood sampling was performed at the age of 6 weeks. Overall, there was an increase in the number of white blood cell, heterophile, eosinophil, monocyte, the ratio between heterophyll and lymphocyte and malondialdehyde (MDA) levels in all treatment groups. While the number of lymphocytes decreased in all treatment groups. Basophils had a significant increase in group C, but there was a decrease in group B and D decreased from control. This indicates that maintenance above temperature > 25 ° C can cause oxidative stress due to heat stress in quail
KADAR MALONDIALDEHID DAN AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE PADA HIPERTENSI DAN NORMOTENSI: Malondialdehyde Level and Superoxide Dismutase Activity in Hypertensive and Normotensive
Get PDFHypertension is a condition of high blood pressure caused by many factors. One of them is endothelial dysfunction indicated from malondialdehyde (MDA) level. The production of MDA is primarily prevented by endogenous antioxidant such as superoxide dismutase (SOD). This study aimed to identify the differences between level of MDA and the activity of SOD in hypertensive and normotensive. It also aimed to identify the correlation between MDA level and SOD activity in both group. This study was conducted in laboratory of animal physiology FMIPA UNJ on June to September 2015. This research used an Ex-Post facto method with a cross-sectional design. A total of 30 serum samples (N1 = N2 = 15) was examined to identify MDA level and SOD activity. Data of MDA level and SOD activity was analyzed by Mann-Whitney test and t-test, respectively. Correlation between MDA level and SOD activity was analyzed by Pearson Correlation test in normotensive group and by Spearman’s Rho test in hypertensive group. Based on the results, mean value MDA level in normotensive was 0,465±0,118 nmol/mL and in hypertensive is 1,279±0,307 nmol/mL. MDA level in normotensive was significantly different than hypertensive (Sig.=0,000). Mean value SOD activity in normotensive was 2,307±0,739 u/mL, and in hypertensive was 2,131±0,422 u/mL. SOD activity was not significantly different in normotensive and hypertensive (Sig.=0,432). In conclusion, there was no correlation between MDA level and SOD activity in hypertensive and normotensive group
STUDI PERILAKU HARIAN DAN KESEJAHTERAAN MONYET HITAM SULAWESI (Macaca nigra Desmarest, 1822) DI PUSAT PRIMATA SCHMUTZER
Get PDFMonyet hitam sulawesi merupakan primata endemik yang hampir punah. Konservasi ex situ monyet hitam sulawesi harus memperhatikan segala aspek yang berhubungan dengan kehidupan hewan tersebut, termasuk aspek perilaku dan kesajahteraan hewan. Penelitian ini bertujuan untuk mengetahui perilaku harian monyet hitam sulawesi berdasarkan jenis kelamin dan umur, serta tingkat kesejahteraannya di Pusat Primata Schmutzer (PPS). Metode yang digunakan dalam penelitian ini adalah metode deskriptif dengan teknik observasi. Analisis data perilaku harian monyet hitam sulawesi berdasarkan jenis kelamin dan umur dilakukan dengan uji Mann- Whitney, sedangkan analisis data tingkat kesejahteraan hewan dilakukan secara deskriptif. Hasil penelitian menunjukan bahwa terdapat perbedaan perilaku harian monyet hitam sulawesi berdasarkan jenis kelamin, yaitu pada perilaku bergerak, mencari makan, istirahat, dan sosial. Hasil penelitian juga menunjukan terdapat perbedaan perilaku harian monyet hitam sulawesi berdasarkan umur, yaitu pada perilaku bergerak, istirahat, dan sosial. Berdasarkan hasil analisis, tingkat kesejahteraan monyet hitam sulawesi di PPS termasuk dalam kriteria baik
POTENSI KELELAWAR SEBAGAI VEKTOR ZOONOSIS: INVESTIGASI BERDASARKAN KEANEKARAGAMAN JENIS DAN PERSEPSI MASYARAKAT TERHADAP KEBERADAAN KELELAWAR DI KOTA TANGERANG SELATAN
Get PDFZoonosis from bats to human in urban areas is a problem that must be investigated.This study aims to determine the species diversity in Tangerang Selatan City and human perception of bats. The study was conducted in July 2015 to November 2015. Bats were caught by mist nets and harp traps. Traps was placed purposively based on bats traffic at each study site. There are 3 sampling locations, each location, has been done for 4 nights with 3 mist net. Bats are found in South Tangerang City consisting of 5 spicies, namely: Cynopterus brachyotis, Cynopterus horsfieldii, Cynopterus titthaecheilus, Macroglossus sobrinus and Myotis muricola, with an index of species diversity was (H ‘= 1.68). Human perception and behavior in relation to the spread of zoonosis are at high scores (> 75), which means that the perception of the bat can keep them away from a zoonotic disease caused by bat
INDUKSI MUTASI PADA PISANG (Musa sp. - ABB) cv. KEPOK DENGAN IRADIASI GAMMA SECARA IN VITRO
Get PDFBanana (Musa sp. - ABB) cv. Kepok is one of type banana processed that have a very potential commodities fruit developed to support food survival. The purpose of this study was to knowing the effect of gamma irradiation on the growth of banana plants cv. Kepok in vitro. This study was conducted in October 2014 – October 2015 in Plant Tissue Culture Laboratory, Biological – Science UNJ. The methods used was experiment with fully randomized design. Factors that tested was 6 gamma irradiation doses (0, 20, 30, 40, 50, 60 Gy) with 10 repetition. Observation of phenotypic generate diverse characters on the growth of the number of shoots and leaves. Gamma irradiation dose of 50 Gy is doses most inhibits the growth of character. Mutations that occur in banana plantlets cv. Kepok generated by the treatment doses gamma irradiation induced mutation is random
HUBUNGAN KARAKTERISTIK LANSKAP DENGAN PREFERENSI SITUS OVIPOSISI KUPU-KUPU DI RUANG TERBUKA HIJAU KAWASAN JAKARTA TIMUR
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Urban area is dominated by land that functioned of the interest of economy and settlement, but only a few land allocated for wildlife. The butterflies was one of the wildlife that could be found in urban areas.The remaining habitat that can be used by butterflies assumed confined to the urban green space. Based on its life cycle, the butterflies having an initial phase (egg to larvae) is a phase which is generally require specific habitat. This research aims to determine the relationship between landscape characteristic and oviposition site preferences of butterfly. This research was conducted on April to June, 2014 at fifteen urban green spaces in East Jakarta by using descriptive survey technique. Landscape characteristics measured include area, perimeter, lawn area, closed vegetation area, open vegetation area, non vegetation area. Landscape characteristic not only measured from urban green space, but also measured from the area around urban green space within the scope of 100 meters buffer. Results show that there is a relationship between landscape characteristic and oviposition site preferences. Significant positive correlation between the abundance and area correlation coefficients rs (0,546), open vegetation area rs (0,758) and non vegetation buffer area rs (0,688). There was no significant correlation between the abundance with perimeter area, lawn area, closed vegetation area, non vegetation area, lawn buffer, open vegetation buffer and closed vegetation buffer.
Keywords: caterpillar,landscape characteristic, oviposition site, preferences, urban green spac
PENGAMATAN AKTIVITAS TIKUS WISTAR JANTAN DENGAN ALAT OPTO- VARIMEX® PADA KONDISI DIET TINGGI MINYAK TRANS
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Excessive energi consumption of high-fat diet increase the body’s adipose tissue which also affect the increased secretion of leptin, this is because leptin secretion is proportional to the mass of adipose tissue. Leptin activates the lateral hypothalamus as the center of hunger and feeding activity. The aim of this study was to obtain the relationship between high trans fat feeding on male Wistar rats and feeding activity. This method uses male Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% trans fat, the second group was given additional feed 40% trans fat, a third group was given additional feed 60% trans fat, and the last as a control group. This feed was given once a day for 70 days. Observation of activities perform using Opto-varimex® auto-track system ver. 4.31. The results showed an corellation between activity based doses of trans fat.
Keywords: Opto-varimex®, Trans fat, Activity, Wistar rat
PENGARUH AKTIVITAS FISIK MAKSIMAL DAN EKSTRAK DAUN BAMBU MANGGONG (Gigantochloa manggong) TERHADAP AKTIVITAS KATALASE PADA HATI TIKUS PUTIH (Rattus norvegicus)
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Gigantochloa manggong, one of endemic bamboo plant in Indonesia is suspected have potensial as an exogenous antioxidant. Exogenous antioxidant can help the activity of endogenous antioxidant on the body when overtraining occurs. Antioxidant can be measured by catalase enzymes activity. This present study was carried out to determine the effect of maximum physical activity and leaf bamboo extract on catalase activity in liver organ of rats. This research used experimental method with completely randomized design (cRD). The test groups were the control rats (E0R0), rats induced leaf extract (E1R0), rats treated swimming activity (E0R1) and rats induced leaf extract and swimming activity (E1R1). Data were analyzed by the two-way ANOVA statistical test. Bamboo leaf extract turned out to be non-toxic and leaf extract contained flavonoids, alkaloids, triterpenoids dan saponins. Catalase units in the control group = 1.00 unit/ml, the group which induced leaf extract = 0.89 unit/ ml, the group which treated maximum physical activity = 0.78 unit/ml and the group which treated maximum physical activity and induced leaf extract = 0.56 unit/ml. Based on statistical test, catalase activity no effect (p> 0,05) on rats. It was concluded that maximum physical activity can’t decreased catalase activity, leaf bamboo extract can’t increased catalase activity and there was no effect between maximum physical activity and manggong bamboo leaf extract on catalase activity in liver organ of rats.
Keyword : antioxidant, catalase, manggong bamboo, physical activit
PERTUMBUHAN DAN AKTIVITAS ANTIOKSIDAN DARI MIKROALGA
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Microalgaebiomass containschemicalcomponentssuch as vitamin, proteins, ω-3 and ω-6 polyunsaturated fatty acid and pigments. components with antioxidant activities can be found in only a few species of algae. The potential species of microalgae who have compounds act as antioxidant are Porphyridium cruentum and Chlorella sp. This study aimed to know the growth and antioxidant activity of P. cruentum and Chlorella sp. with the treatment of different cultures. Experimental method and completely randomized design was used as the research method. Antioxidant activity (Ic50 value) was measured by DPPH method (2,2-diphenyl-1-pikrilhidrazil). SPSS 16.0 is used to analyze the data with General Linier Model (GLM) Univariate. The results of GLM Univariate test for cell density showed that there was significant different for cell density in species factors (p= 0,000), but there was no significant different for cell density in culture factors (p= 0,375), and GLM Univariate test for antioxidant activity showed that no significant different for antioxidant activity in species factors (p= 0,522) but siginificant different for antioxidant activity in culture factors (p= 0,001). There was significant different for growth in P. cruentum and Chlorella sp. but no significant different in indoor and outdoor culture, as well as there was no significant different antioxidant activity in two species but significant different in indoor and outdoor culture.
Keywords: growth, antioxidant activity, indoor cultivation, outdoor cultivation
ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR
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Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial compounds. coral and sponge-ssociated bacteria are potential producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with Staphylococcus aureus, Bacillus subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate was isolated from corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid.
Key words: antibacterial activity, association, characterization, coral, isolation, spong