Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
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Ketersediaan Fosfor pada Tanah Andisol untuk Jagung (Zea maysL.) oleh Inokulum Bakteri Pelarut Fosfat
Get PDFAndisols has relatively low phosphorus availability due to its adsorption by allophane. Phosphate solubilizing bacteria (PSB) increases the availability of P via release of adsorpted-P. The aims of this research were to determine: 1) anorganicP solubilization, 2) organic-P mineralization, 3) blocking of Andisols adsorption site, and 4) effective PSB inoculant. The research was arranged in completely randomized design, with PSB inoculant as treatment. Variables observed were solubleP, mineralize-P, adsorpted-P, pH, total acidity, PSB population, phosphatase and phythase activity, relative surface charge, and maize’s growth component. The result showed that PSB inoculation increased soluble-P from 30 to between 150 and 195 ppm P, increased mineralize-P from 23.7 to between 63.6 and 91.7 ppm P, and decreased P-adsorption from 95 to between 36 and 13%. PSB inoculation decreased the Andisols pH, increased the total acidity, PSB population, the phosphatase and phytase activity, and PSB had relatively high of relative surface charge (69%). The PSB inoculation increased maize P absorption in the range of 70 and 75 mg P plant-1, and increased relative agronomic effectiveness (RAE )between 145 and 150%. Liquid and solid PSB inoculant had no different effect in increasing maize growth. Keywords: Andisol, P release, phosphate solubilizing bacteria, phosphatase, phytas
Pemberian Mulsa dalam Budidaya Cabai Rawit di Lahan Kering: Dampaknya terhadap Hasil Tanaman dan Aliran Permukaan
Get PDFThe problem of plant cultivation in dry upland with slope of >15° is the high soil erosion that result in high rate of sedimentation in the downstream of watershed. One way of overcoming this problem is by utilization of mulches. The objective of the experiment was to study the effect of mulch application on growth and yield of chilli (Capsicum frutescensL.).The experiment was conducted from January 2010 until June 2011 at Selopamioro micro watershed at Imogiri Subdistrict, Bantul District, Special Region of Yogyakarta. The experiment was arranged in a randomized complete block design consisted of four mulch treatments (rice straw, litter, plastic/silver black polyethylene, and without mulch) and four replications. The observed variables were the growth parameter (plant height), soil moisture content, soil temperature, and yield (number and weight of chilli). The result showed that application of mulches did not affect plant height and yield of chilli, but increased number of fruit. The best mulch for chilli crops in upland area was rice straw, that yielded the highest increase in number of fruit. Mulch as a soil conservation practice reduced runoff coefficient, while dicharge and extended of the reponse time were reduced only at rainfall less than 21 mm.Keywords: Capsicum frutescensL.,mulch, runoff, upland are
Pemanfaatan Mikrob Pelarut Fosfat untuk Mengurangi Dosis Pupuk P Anorganik pada Padi Sawah
Get PDFABSTRAKMikrob pelarut fosfat (MPF), meliputi bakteri pelarut fosfat (BPF) dan fungi pelarut fosfat (FPF), berpotensi dalammeningkatkan ketersedian P dan meningkatkan efisiensi penyerapan P dari pupuk P anorganik. Tujuan penelitian adalahmengisolasi dan menyeleksi MPF, menguji efektivitas MPF dalam meningkatkan P tersedia dan mengurangi dosis pupuk Panorganik pada sistem budidaya pengelolaan tanaman terpadu (PTT) dan system of rice intensification (SRI). Percobaanlaboratorium meliputi isolasi dan seleksi MPF, uji indeks pelarutan P, uji pelarutan P dari sumber P sukar larut dan ujiantagonistik. Percobaan lapangan meliputi perlakuan dosis pupuk Panorganik yaitu 100%, 75%, 50%, dan kombinasi BPF,FPF, BPF+FPF. MPF diinokulasi menggunakan metode perendaman akar padi. Hasil percobaan laboratorium menunjukkanisolat bakteri BPFA5 (Pseudomonas aeruginosa) dan isolat fungi FPFE1 (Aspergillus niger) memiliki kemampuan pelarutanP lebih tinggi dan kompatibel dalam satu kultur. Hasil percobaan lapangan menunjukkan bahwa perlakuan sistem budidayaSRI lebih unggul dibandingkan PTT berdasarkan pertumbuhan, komponen hasil, hasil gabah, dan serapan hara P gabah.Perlakuan 75% dosis pupuk P anorganik+MPF (bakteri dan fungi) pada sistem budidaya SRI menghasilkan jumlah anakanproduktif, hasil gabah, dan serapan hara P gabah yang lebih tinggi dibandingkan perlakuan lainnya. Aplikasi mikrob pelarutP dapat mengurangi dosis pupuk P anorganik sampai 50% dan meningkatkan hasil gabah dan serapan hara P jerami dangabah.Kata kunci: bakteri pelarut fosfat, fungi pelarut fosfat, pengelolaan tanaman terpadu, system of rice intensificatio
Gamma Irradiation on Growth and Development of Amorphophallus muelleri Blume.
Get PDFABSTRACT Iles-iles (Amorphophallus muelleri Blume) produces apomictic seeds lead to low genetic variation. In order to induce genetic variation, germinated seeds were exposed to Gamma irradiation (Co-60) at doses of 10 to 100 Gy. Seed irradiation was conducted at Center for the Application of Isotope and Irradiation Technology -National Nuclear Energy Agency (CAIRT), Indonesia. Morphology and yield of M1 generation were observed. Results showed that irradiation at a dose of 10 Gy close to LD50 with survival rate 56%. Gamma irradiation at a dose of 10 Gy delayed seeds germination. Germination rates gradually increased and reached maximum at 4 weeks after planting (WAP) for control plants, and 14 WAP of irradiated plants. At 16 WAP, germination rate of 10 Gy irradiated plants was 56% and 84% for those of control plants. Irradiation induced chimera as indicated by short petiole, variegated and abnornal shape of leaflets. Some irradiated plants entered dormancy at 8-10 weeks later than control ones. Prolong vegetative periode lead the plants to produce heavier corms. This study revealed the possibility to induce variation of A. muelleri by using gamma irradition. Keywords: Amorphophallus muelleri, gamma irradiation (Co-60), morphological variation, mutation breedin
We investigated the effect of sector (position in canopy) on translocation and distribution of 13C-photosynthates in mangosteen trees and related the findings to previous analyses of fruit quality. Our experiment was conducted on three 25-year-old mangosteen trees. Tree canopies were divided into 9 sectors based on height (bottom, middle, top) and width (inner, center, outer). One branch from each sector was labeled with 13CO2 in December 2003. Immediately after labeling, 13C concentration in leaves from middle sectors was higher than that in leaves from other positions. 13C concentration in all leaves decreased rapidly for 24 h after 13C feeding, followed by a gradual decrease. In contrast, 13C concentration increased over time in the pericarp and aril of fruits. Translocation of 13C-photosynthates into fruit was high in Sectors 4 and 5, and in top positions (Sectors 7 to 9). At 96 h after 13C feeding, the highest distribution ratio of 13C-photosynthates was observed in stems, followed in descending order by pericarp, leaf, and aril. 13C distribution ratio in the aril was generally highest in fruits from inner and center positions. The relationship between partitioning of photosynthates and quality of mangosteen fruit, which differs among sectors, has been discussed. Keywords: fruiting positions, Garcinia mangostanaL., photosynthate partitioning, 13C, tree branches
Get PDFWe investigated the effect of sector (position in canopy) on translocation and distribution of 13C-photosynthates in mangosteen trees and related the findings to previous analyses of fruit quality. Our experiment was conducted on three 25-year-old mangosteen trees. Tree canopies were divided into 9 sectors based on height (bottom, middle, top) and width (inner, center, outer). One branch from each sector was labeled with 13CO2 in December 2003. Immediately after labeling, 13C concentration in leaves from middle sectors was higher than that in leaves from other positions. 13C concentration in all leaves decreased rapidly for 24 h after 13C feeding, followed by a gradual decrease. In contrast, 13C concentration increased over time in the pericarp and aril of fruits. Translocation of 13C-photosynthates into fruit was high in Sectors 4 and 5, and in top positions (Sectors 7 to 9). At 96 h after 13C feeding, the highest distribution ratio of 13C-photosynthates was observed in stems, followed in descending order by pericarp, leaf, and aril. 13C distribution ratio in the aril was generally highest in fruits from inner and center positions. The relationship between partitioning of photosynthates and quality of mangosteen fruit, which differs among sectors, has been discussed. Keywords: fruiting positions, Garcinia mangostanaL., photosynthate partitioning, 13C, tree branche
Konservasi In Vitro Pamelo (Citrus maxima (Burm.) Merr.) melalui Pertumbuhan Lambat
Get PDFIndonesia is one of the countries which have abundant germplasm of pummelo (Citrus maxima (Burm.) Merr.). Thepummelo germplasm must be conserved to prevent its extinction due to biotic and abiotic stresses. In vitro conservationusing slow growth technique can be considered as an alternative of ex-situ conservation. Two experiments were conductedto obtain suitable medium to conserve pummelo in vitro. The first experiment was conservation using modified concentrationof MS and sucrose. MS medium concentrations were 1/2MS and MS, while sucrose concentrations were 0, 1, 2 and 3%. Thesecond experiment was conservation using osmoticum and retardant in MS medium. There were six combinations of MSmedium supplemented with osmoticum and retardant, i.e. MS + sucrose 3%, MS + sucrose 3% + paclobutrazol 7.5 ppm, MS+ sucrose 3% + paclobutrazol 15 ppm, MS + sorbitol 2%, MS + sorbitol 2% + paclobutrazol 7.5 ppm, MS + sorbitol 2% +paclobutrazol 15 ppm. The results showed that reducing MS medium and sucrose consentration decreased leaf number andshoot length but increased root number and length. The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length. Based on the planlet visual and inhibition of growth through the decrease of leaf number, shoot and root length, the best medium to preserve pummelo was MS + sorbitol 2% + paclobutrazol 7.5 ppm.Keywords: Citrus maxima, osmoticum, preservation, retardant, slow growt
Karakter Agronomi dan Fisiologi Varietas Cabai Merah pada Kondisi Cekaman Genangan
Get PDFThe research objective was to study the agronomic and physiological characteristics of red chilli (Capsicum annuumL.) varieties under waterlogging stress. These studies were conducted at two locations: in Palembang, South Sumatra, from February to August 2010 and in Bogor in West Java, from September to November 2010. Experiments in Palembang was using split plot design with three replications. The main plot was waterlogging treatment for 1, 2, 3 and 4 days. The subplot was red chilli varieties: Kiyo F1, Riawan, Ferosa, Bravo F1 and Laris. Experiments in Bogor was conducted without the use of experimental design; the same plant varieties were waterlogged for 2 and 4 days. The results showed that waterlogging caused root damages on all varieties. Kiyo F1 had better agronomic characters and high levels of tolerance than other varieties. The content of ethylene in all varieties increased with increasing time of waterlogging, except for Laris. In all varieties both leaf chlorophyll and tissue N content tend to decline with the increase in waterlogging duration. However Bravo F1 experienced an increase in the chlorophyll content whereas Kiyo F1 had an increase in N in the plant tissue. Keywords: agronomic character, physiological character, red chili, waterloggin
Aktivitas Kitinase dan Peroksidase dari Ekstrak Kasar Protein Asal Kalus dan Berbagai Jaringan Tanaman Trichosanthes cucumerina var. anguina
Get PDFChitinase and peroxydase are important bioactive proteins or are specific enzymes that are related to plant resistance to pathogens. The aims of the research were to analyze the chitinase and peroxidase activities of crude protein extract from calli, stem, leaves and roots of T. cucumerinavar. anguina. In the first experiment, chitinase and peroxydase activities were analyzed from in vitro calli, leaves and roots obtained from 2-month-old of field grown plants. The media for calli induction were Murashige and Skoog medium with addition of 1 µM NAA + 1 µM BA, 2 µM NAA + 2 µM BA, 3 µM NAA + 3 µM BA, or 4 µM NAA + 4 µM BA. In the second experiment, the chitinase and peroxydase activities from crude protein extract of roots, stems and leaves were analyzed. The extracts were from 3-week-old seedling (less than a month), 1-month and 2-month-old plants. The first and the second experiment results showed that crude protein extracts of plant roots from the field grown plants had the highest chitinase and peroxidase activities. Stem of field grown plants had the similar level of chitinase activities with the plant roots. Chitinase activities of in vitro calli were not significantly different from those of plant roots so that it could be used as an alternative for plant roots in studying chitinase from T. cucumerinavar. anguina. Chitinase activities in crude protein extracts of roots appeared constant whereas peroxidase tend to increase with plant age. Keywords: calli, enzyme activities, leaves, roots, ste
Induksi Ginogenesis melalui Kultur Multi Ovule Slice dan Kultur Ovary Slice Dianthus chinensis
Get PDFCallus induction was studied in five genotypes of Dianthus chinensis using 2.4 D and NAA. Calluses can be obtainedfrom unfertilized ovule culture and ovary culture. The aim of the research was to study gynogenic potential and responseof Dianthus chinensis through ovule slice and ovary slice culture for obtaining haploid plants. Five genotypes of Dianthuschinensis and five media were used in ovule slice culture and two genotypes and three medium were used in ovary culture.Flower buds in the 7th stage were incubated for the purpose of dark pre-treatment at 4 oC for one day. Ovules and ovaries wereisolated and cultured in induction medium. Cultures were incubated for the purpose of dark pre-treatment at 4 oC for seven days, followed by 25 oC light incubation. The result showed that 2.4D was better than NAA in inducing callus. Percentage of regenerated calluses were produced in V11, V13 and V15 genotypes in M7 medium (MS + 2 mg L-1 2.4D + 1 mg L-1 BAP + 30 g L-1 sucrose and M10 medium (MS + 1 mg L-1 2.4D + 1 mg L-1 BAP + 20 g L-1 sucrose). All calluses originated from ovule and ovary cultures flowered prematurely. Double haploid (V11-34) were obtained from ovule slice culture based on PER (peroksidase) and EST (esterase) isoenzym marker.Keywords: ovule slice culture, ovary slice culture, callus, Dianthus sp., haploi
Dampak Bokashi Kotoran Ternak dalam Pengurangan Pemakaian Pupuk Anorganik pada Budidaya Tanaman Tomat
Get PDFThe objective of this research was to study the effect of bokashi livestock on the growth and yield of tomatoes. The experiment was conducted in Bandar Lampung from October 2009 until February 2010. The experimental design was randomized complete block design with 3 replications. The treatments were: control; inorganic fertilizers at a recommended rate of (kg ha-1) 135 N, 75 P2O5, and 110 K2O (RR); chicken manure bokashi + RR; chicken manure bokashi + 50% RR; cow manure bokashi + RR; cow manure bokashi + 50% RR; sheep manure bokashi + RR; sheep manure bokashi + 50% RR; horse manure bokashi + RR; horse manure bokashi + 50% RR. Each bokashi treatment was applied at the rate of 20 ton ha-1. The result of experiment showed that chicken manure bokashi was the best among bokashi livestock. The application of 50% RR combined with chicken, cow, sheep or horse manure bokashi gave a higher yield than treatment with inorganic fertilizer at recommended rates. These results demostrated that the application of bokashi livestock could potentially reduce the use of inorganic fertilizers while maintaining higher yield. Keywords: animal manure, inorganic fertilizer, bokashi livestock, tomatoe