Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
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Efektifitas Herbisida Formulasi pada Gulma Air di Lahan Rawa Tadah Hujan, Rawa Payau dan Saluran Drainase
Get PDFA series of studies had been carried out to create new types of herbicides. The studies had found six formulations ofherbicides. This study aimed to determine the effectiveness of herbicides formulation in controlling aquatic weeds. Theexperiment used a randomized complete block design with seven herbicide formulations as treatments and three replications.The seven formulations of herbicide were: Unihaz 1, Unihaz 2, Unihaz 3, Unihaz 4, Unihaz 5, Unihaz 6, and a control(glyphosate 2 kg ha-1). The results of the study found that there were 21 species of weed in rainfed swamps, ten species inbrackish swamps and one species namely water hyacinth (Eichhornia crassipes) in drainage channel. Herbicide formulationsof Unihaz 3, 4, 5, and 6 were effective in controlling aquatic weeds in rainfed swamps. Herbicide formulations of Unihaz 2, 3and 5 were effective in controlling aquatic weeds in brackish swamps. All herbicides formulations were effective in controllingweeds in drainage channels. Moreover, herbicide formulation of Unihaz 5 was more effective in controlling aquatic weeds inrainfed swamps, brackish swamps and drainage channels than other formulations, and it was not significantly different fromthe use of a glyphosate dose of 2 kg ha-1.Keywords: Eichhornia crassipes, fermented coconut water, organic herbicide, new types of herbicide
Optimasi Konsentrasi Asetosiringon dan Higromisin dalam Transformasi Genetik Padi Fatmawati dengan Perantaraan Agrobacterium tumefaciens
Get PDFProtocols for genetic transformation of rice have been widely developed, however the protocols are not universal and inapplicable for all types of rice plants directly. Transformation protocol on rice cv. Fatmawati needs to be developed to generate transgenic lines. The present research was carried out to optimize genetic transformation protocol in rice cv. Fatmawati mediated by Agrobacterium tumefaciens harboring pCambia1301 construct using immature embryo as an explant. The experiment was arranged in a completely randomized design. Factors influencing efficiency of transformation, i.e., sensitivity of callus to hygromycin antibiotic, acetosyringone concentration used in cultivation medium, hygromycin concentration for transformant selection were optimized. The results showed that genetic transformation of rice cv. Fatmawati mediated by A. tumefaciens using immature embryos have been successfully carried out with several parameters. Addition of 100 µM acetosyringone in co-cultivation medium and 30 mg L-1 hygromycin for transformant callus selection were optimal for genetic transformation of rice cv. Fatmawati mediated by A. tumefaciens. Transformation efficiency was found to be 7.84% based on the lines carrying the hpt gene. This result would be a valuable reference in genetic transformation of rice cv. Fatmawati using target genes.Keywords: immature embryo, Oryza sativa, pCambia1301, transformation efficienc
Komposisi Media Pertumbuhan Protokorm Sebelum Perlakuan Kolkisin untuk Meningkatkan Poliploidi pada Phalaenopsis amabilis (L.) Blume
Get PDFPolyploidy induction in Phalaenopsis amabilis is one of the methods to increase plant genetic diversity. A highly meristematic condition of protocorm induced by cytokinines may improve the success rate of polyploidy induction. This experiment was aimed to study the effect of Benzylaminopurine (BAP), Thidiazuron (TDZ) and coconut water (CW) in a half concentration of Murashige & Skoog (MS) medium before colchicine treatment on growth, multiplication and the success rate of polyploidy induction on Phalaenopsis amabilis protocorm. The experiment used completely randomized design with one factor, medium treatment (15% of CW); 0.51 mg L-1 BAP; 0.50 mg L-1 TDZ; 15% CW + 0.51 mg L-1 BAP; 15% CW + 0.50 mg L-1 TDZ). Each treatment consisted of three replications with five culture bottles of protocorm for each replication and ten protocorms per bottle. The protocorms were incubated for eight weeks in the medium before treated with 50 mg L-1 of colchicine for ten days. The results showed that 15% CW, 15% CW + 0.51 mg L-1 BAP or 0.5 mg L-1 TDZ gave the highest percentage (100%) of alive protocorm. The highest percentage of protocorm multiplication were resulted from 15% CW+0.51 mg L-1 BAP (65.3%) and 15% CW + 0.5 mg L-1 TDZ (62.7%). The best treatment to promote formation of leaves and roots from protocorm was 15% CW. The percentage of polyploid plantlets of 3% was found in plantlet from 15% CW + 0.50 mg L-1 TDZ media treatment while from other treatments could not be detected.Keywords: BAP, chromosome, coconut water, plantlet, TD
Keragaman Kualitas Buah pada Dua Varietas Okra (Abelmoschus esculentus L. Moench) dari Umur Panen Berbeda
Get PDFOkra (Abelmoschus esculentus L. Moench) is a functional vegetables that has high economic value and it is usedas health purposes due to high bioactive compounds and dietary fiber contents. Okra pod is gradually harvested followsflowering time. The purpose of this study was to evaluate the effect of harvest age on Nitrogen (N), Phosphorus (P), Potassium(K), pigment and phenolic contents of two okra varities. The experiment was conducted from October 2017 to February 2018at IPB experimental station in Leuwikopo, Dramaga, Bogor. The experimental field is located at ± 185 m asl with 6033’49.3”S and 106043’30.7” E. The experiment used single factor, namely harvest time, in randomized complete block nested in factorof okra variety with 4 replicates. The varieties of okra were Zahira (red okra) and Naila (green okra), while harvest agesconsisted of 5, 7, 9, and 11 days after the anthesis (DAA). The results of the study showed significant effect of harvest age onN, P, anthocyanin and phenolic contents. Okra pod harvested at 5 DAA, had the highest N, P and anthocyanin levels; whilethe highest phenolic level was obtained from okra pod harvested at 7 DAA. There were interactions effect between harvesttime and okra varieties on chlorophyll and carotenoid contents. The red okra harvested at 5 DAA had the highest content oftotal chlorophyll (536.83 μg g-1) and carotenoid (147.79 μg g-1)
Pengelompokan Klon-Klon Ubi Jalar Berdasarkan Analisis Gerombol, Komponen Utama dan Biplot dari Karakter Morfologi
Get PDFSweet potato is a food crop with high genetic and phenotypic diversities. The objective of this study was to cluster sweet potato clones based on their morphological characters. This research used descriptive method using 25 morphological characters on 28 sweet potato clones and clustered using dice coefficient, principal component analysis and biplot analysis. The results showed that the clones were grouped into two clusters and 14 sub-clusters. Three sub-clusters had high similarity coefficients, i.e., 0.68-0.96, one clone was eliminated, and 11 sub-clusters had a low similarity, i.e., 0.41-0.52. The principal component analysis showed 14 of 25 morphological characters determined the diversity in 27 sweet potato clones with cumulative variance of 70.79%. Biplot analysis showed that 12 characters contributed to cumulative variance of 61.3%. Twelve morphological characters had weak contribution on the characteristics of the clones in quadrant I; meanwhile, characteristics of clones in quadrant II were orange, yellow and white tuber flesh, in quadrant III was dark purple tuber flesh, and in quadrant IV were purple tuber flesh and cortex. Result of the grouping analysis identified clones that were closely related and those distantly related for improvement purposes.Keywords: Ipomoea batatas, cumulative variance, Maluku province, morphology, relationshi
Karakteristik Buah Melon (Cucumis melo L.) pada Lima Stadia Kematangan
Get PDFMelon breeding for fruit quality improvement is important to produce new varieties that meet consumers’ demand. The aim of this study was to elucidate the fruit characteristics of melon at five different maturity stages, involving several genotypes belonging to two cultivar groups: reticulatus and inodorus. The experiments were conducted in three planting seasons: (1) January-March 2015 (13 genotypes), (2) December 2015-February 2016 (56 genotypes), (3) February-May 2016 (9 genotypes). These trials were conducted at the Tajur II experimental station of IPB, Bogor, and each trial was arranged in a single factor randomized complete block design with three blocks. Maturity stages was determined based on fruit rind color and net coverage, and their effects on fruit quality were studied using combined analyses over seasons. Maturity stage significantly affected the fruit length, fruit diameter, flesh thickness, fruit weight, and sugar content. The average weight of the fruits at maturity stage 4 (838.90 g) and 5 (931.79 g) was significantly greater than that of stage 1 (584.42 g). The sugar content increased from maturity stage 2 (5.51 oBrix) to 3 (6.13 oBrix) and to 5 (8.18 oBrix). Fruit weight significantly correlated with fruit length (r = 0.53), fruit diameter (r = 0.85), fruit rind thickness (r = 0.33), and flesh thickness (r = 0.63). Maturity stages affected the quality of melon fruits, and therefore the determination of appropriate harvesting criteria is important in melon cultivations.Keywords: cantalupensis, correlation, inodorus, fruit quality, skin colou
Peran Eksopolisakarida Azotobacter dan Bahan Organik untuk Meningkatkan Nodulasi dan Biomassa Kedelai pada Dua Ordo Tanah
Get PDFExopolysaccharide (EPS) produced by nitrogen-fixing bacteria Azotobacter protect nitrogenase from oxygen. In legume,EPS plays a role in the immobilization of rhizobia to the roots. The objective of this experiment was to study the effect of EPSAzotobacter and organic matter on increasing number of nodules and biomass of soybeans grown in Inceptisols and Ultisols;as well as nitrogen-fixing bacteria population in soybean rhizosphere. The experiment was set up in a completely randomizedblock design with five replications to test combined treatments of two doses of crude EPS and organic matter. Nodule number,shoot dry weight and nitrogen uptake, as well as Azotobacter and Rhizobium population in soybean grown in Inceptisolsfollowing crude EPS and compost application, were higher than those grown in Ultisols. The application of EPS and compostIn Ultisols did not affect the number of nodule and other traits, but in Inceptisols, adding 6.25 g of compost and 20 mL of EPSto each plant increased the number of nodules and shoot weight at 42 days after planting. However, the highest N uptake wasdemonstrated by soybean received 10 mL and 20 mL EPS along with 12.5 g compost
Perbanyakan Pepaya (Carica papaya L.) ‘Sukma’ In Vitro dari Eksplan Tunas Pucuk sebagai Respon terhadap BA dan NAA
Get PDFPapaya is one of tropical fruits native to Southern Mexico and which have been cultivated in Indonesia for a longtime. Papaya is usually propagated by seeds. Therefore, the offsprings are not true-to-type. This study was conducted todevelop a protocol of in vitro propagation of papaya ‘Sukma’ from shoot tips of in vitro germinated seeds as explants. Seedswere extracted from fruit that physiologically ripe and it germinated on MS basal medium. The experiment was set up in arandomized block design with culturing day as blocks (four blocks) and various concentrations of BA (0.0, 0.5, 1.0, 1.5, and2.0 mg L-1) in MS medium enriched with NAA 0.5 mg L-1 as treatment. Shoot tips in the MS medium without plant growthregulator as control so there was six treatments and 24 observation units. Each observation units contained five culturevessels and each culture vessels contained four explants. The results showed that the highest number of shoots, number ofleaves, number of nodes, and percentage of explant forming callus were obtained by BA 1.0-1.5, 1.0-2.0, 0.5-2.0, and 1.0-1.5 mg L-1, respectively. The highest percentage of explant forming roots were obtained in medium without BA. Analysis ofregression showed that the optimum concentration to get the highest number of shoots and leaves were BA 1.31 and 1.35 mgL-1, while explants will be rooted in medium without BA. Thus, in vitro propagation for papaya ‘Sukma’ should be conductedin two steps, i.e. for shooting and rooting growth
Evaluasi Klon-Klon Tebu Potensial di Lahan Kering
Get PDFThe candidates for new superior varieties are expected to be able to increase the sugar yield in the upland area. Germplasm evaluation has indicated some clones had potentially high sugar yield in upland area. The study was conducted at Asembagus Experimental Station, Situbondo Regency, East Java, from December 2016 to November 2017. The study aimed to obtain sugarcane clones with higher sugar yield than existing varieties on upland area. Twelve potential sugarcane clones (17, 87, 90, 104, 212, 351, 354, 451, 452, PBG 2, 386 SOF 1118, and 400 SOF 1132) and 2 check varieties (PS 881 and Cenning) were arranged in a randomized block design with three replicates. The results showed that the sugarcane production of 104, 212, and PBG 2 clones (97.42-98.26 ton ha-1) were not different from productivity of Cenning (105.42 ton ha-1) but they were higher than that of PS 881 (75.68 ton ha-1). The sugar content of 104 and PBG 2 clones (9.43-9.46%) was higher than Cenning (6.94%) but lower than PS 881 (11.66%). The sugar yields (9.16-9.24 ton ha-1) of 104 and PBG 2 clones were higher than Cenning (7.47 ton ha-1) but they were not different from PS 881 (8.84 ton ha-1)