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Kasus Kolera Pada Itik
Full text linkTelah ditemukan kasus kolera pada itik yang diperiksa di Laboratorium Patologi Unggas, Fakultas Kedokteran Hewan, lnstitut Pertanian Bogor antara tahun 1992 hingga 1994.Kebanyakan itik terserang kolera yang akut, tanpa gejala klinis dan kematian mendadak. Beberapa itik terserang secara kronis dengan gejala klinis sesak nafas dan kebengkakan pada gejala kepala.Gambaran patologi anatonli yang tampak berupa titik perdarahan pada epikardium enteritis kartarhalis, hepatitis nekrotikan rqilier, linlpa membengkak, peradangan pada kantung udara, pecah kuning telur, serta peradangan pada ovariurn dan sinus. Dari darah jantung dan sumsum tulang itik dapat diisolasi bakteri Pasteureulla multocrda. Kelinci yang disuntik dengan suspensi bakteri mati dengan gejala septikemia yang ditandai dengan perdarahan padaepikardium dan selaput serosa. hiperemi dan pembengkakan umum serta enteritis
The IPB-I Reagent As An Alternative Tool To Detect Subclinical Mastitis
Full text linkMastitis subklinis hanya dapat dideteksi melalui pemeriksaan mikrobiologi dan penghitungan jumlah sel radang terhadap contoh susu. Penyakit ini sangat merugikan peternak karena produksi susu menurun dan seringkali berkembang menjadi mastitis klinik atau kronis yang berakibat penyingkiran sapi lebih awal. Melakukan deteksi dini dengan pereaksi terhadapcontoh susu dapat memperkecil resiko tersebut. Ujimastitis subklinis di lapang yang ada sampai saat ini,seperti Calijbrnia Mastitis Test (CMT), masih jarangdilakukan karena harga pereaksinya cukup lnahal dansulit diperoleh di pasaran. Untuk memperoleh suatuteknik yang cepat dan pereaksi untuk uji mastitis subklinis di lapang yang relatif lebih mudah, murah dan bahan-bahannya mudah diperoleh di pasaran, maka dikembangkanlah pereaksi yang diberi nama "IPB- I ". Dari penelitian ini diperoleh data bahwa sensitivitas IPB- I, CMT, Whiteside Test (WST), Aulendorfer Mastitis Probe (AMP) mod- 1 dan AMP mod-2 berturut-turut 0,99; 0,92; 0,94; 0,92 dan 0,94. Sedangkanspesifisitasnya berturut-turut 0,92; 0,37; 0,32; 0,47 dan0,89. Nilai prediksi (predictive valz~e)I PB- I, CMT, WST, AMP mod-1 dan AMP mod-2 berturut-turut 0,95; 0,99; 0,97; 0,99 dan 0,97. Nilai Keterhandalan IPB-I lebih tinggi dibandingkan dengan pereaksi lainnya. Namun nilai prediksi untuk pereaksi masih harus diperbaiki
Morphology and Histomorphometry of Testis And Epididymis of Kacang Goat (Capra sp.) and Local Sheep (Ovis sp.)
Full text linkPenelitian ini bertujuan untuk mempelajari morfologi dan histomorfometri testis dan epididymis kambing kacang (Capra sp.) dan domba lokal (Ovis sp.) dengan umur 1-1,setahun. Secara makroskopik, keliling, berat dan volume testis kambing kacang lebih kecil (
Hemorrhagic Septicaemia In Alabio Ducks Infected With Pasteurella multocida By Various Routes
Full text linkSeratus ekor itik Alabio yang berumur lima rninggu dibagi menjadi empat kelompok berdasarkan rute infeksi yaitu intravena (i.v.), intramuskular (i.m.) intratrakhea (i.t.) dan kontrol (K). Kelompok perlakuan diinfeksi dengan bakteri P. multocida yang diisolasi dari itik dengan dosis 2x 105 cfu mL. Semua itik dinekropsi setelah satu, tiga, enam, 12 dan 24 jam infeksi. Itik-itik dari Kelompok i.v. menunjukkan septikemia hemorhagika, sedang itik dari Kelompok i.m. dan i.t. menunjukkan kerusakan yang sama tiga jam p.i. Semakin lama waktu pengamatan, semakin parah proses kerusakannya. Pada Kelompok Kontrol tidak ditemukan kerusaka
Maturing Singly Versus in Groups on Ovine Oocyte Meiosis
No full textThe effect of maturing systems (single vs groups) of ovine oocytes was studied to examine the oocyte development in vitro. Oocytes were aspirated from follicle ovaries collected at local abattoirs Cibinong West Java, using a 18-G needle. Aspiration medium consisted of H 199 + 2 % FCS + 50 μg/ml Heparin and the maturating medium (IVM) consisted of B 199 - 10 % FCS + 10 μg/ml FSH + 10 μg/ml hCG + 1 μg/ml Estradiol. The oocytes collected were divided into three groups and treated separately as follows: T1) oocytes were matured singly in 50 μl IVM medium. T2) every five oocytes was matured in 50 μl IVM medium and T3) every 10 oocytes was matured in 50 μl IVM medium. All oocytes were maintained in incubator with 5 % CO, and high humidity at 38 °C for 20 h. The resulting ova were stained in 1 % lacmoid then examined for meiosis division under a microscope. There was a signiticant effect among treatments on the proportion oocytes reaching metaphase II (
Native Virus Challenge Test Against Vaccinated Chickens With Commercial Active and Inactive IBD Vaccines
Full text linkVaccination trial were conducted on two groups of broiler day-old-chick (DOC) using active and inactive IBD vaccines. First Group was further divided into two groups: one received active IBD vaccination at 7 days of age, and the other received oral vaccination at 7 and 21 days of age, respectively. Both subgroups were then challenged against native IBD isolate, K-5, at 21 days of age. The second Group was arranged similar to the first group, but the second Group received inactive IBD vaccination subcutaneously, at 21 days of age. At 35 days of age, all chickens were challenged against native IBD isolate, K-5. The group which received active vaccine showed pathological change of the bursa, correlated to the bursa/body weight indices less than 0.70 (20 %) and the bursa lesion score (BLS) was 1.56. This pathological change was more obvious after second application of the vaccine. The group which received active and inactive vaccines revealed immune responses with mild lesion in the bursa. This im-munity could not protect the chickens after challenged with K-5 isolate, correlated to the bursa/body weight indices less than 0.70 (100 %) and BLS was 4.0. This results show that K-5 isolate belong to different subtype or variant
Sperm motility is the main problem for honeybee\u27s reproduction both in natural and in artificial insemination. Sperm produced only by sexually mature drone. Drone with high semen volumes can be seen by the external morphology like hairy in back and the existence of yellow stripes on their black abdomen. Semen samples were collected from the drone by the massage technique; these consist of massaging the abdomen of drone. This massage proceeded until penis and semen cream to yellow color, was produced. Semen taken out with spuit (3 ml) that contain a modification dilution media called "Kiev". Semen samples were collected; volume, abnormalities and motility were measured. The sexually mature drones were observed in 99.06% (213/215) drones with rare hair in back and 76.28% (164/215) with existence of the yellow stripes in the black abdomen. The color of sperm is yellowish white, which is different from mucous that is white. The average volume of semen samples from each drone is 1.11 μl. The average length of sperm is 217.57 μm (107.50-412.50 μm). The average length of sperm is 7,52 μm (5-1O μm). It\u27s flipped tails, broken tails, double tails, and double heads with an average of 19.83%, 12.75%, 6.42%, and 2,25% respectively can determine abnormalities of the sperm. To conduct the artificial insemination, drone sperm should always be available. For this reason sperm should be preserved in an optimal condition storage temperature (5°C, 27 °c and 37°C) and glucose concentration (0%, 0,3%, 0,6%, and 0,9%) containing in the dilution media. This condition can be affecting the sperm motility. The highest motility is achieved when the sperm is kept in 0,9% glucose concentration and storage in 5 °C temperature. In this condition 0.19x106 sperm/ml can survive up to 36 hours. Moreover, the higher concentration of sperm motility up to 36 hours is achieved when sperm is kept in 5 Celsius temperature with different leves of glucose in dilution media. However statistically interaction between glucose concentration and temperature levels does not give significant affects.
No full textSperm motility is the main problem for honeybee's reproduction both in natural and in artificial insemination. Sperm produced only by sexually mature drone. Drone with high semen volumes can be seen by the external morphology like hairy in back and the existence of yellow stripes on their black abdomen. Semen samples were collected from the drone by the massage technique; these consist of massaging the abdomen of drone. This massage proceeded until penis and semen cream to yellow color, was produced. Semen taken out with spuit (3 ml) that contain a modification dilution media called "Kiev". Semen samples were collected; volume, abnormalities and motility were measured. The sexually mature drones were observed in 99.06% (213/215) drones with rare hair in back and 76.28% (164/215) with existence of the yellow stripes in the black abdomen. The color of sperm is yellowish white, which is different from mucous that is white. The average volume of semen samples from each drone is 1.11 μl. The average length of sperm is 217.57 μm (107.50-412.50 μm). The average length of sperm is 7,52 μm (5-1O μm). It's flipped tails, broken tails, double tails, and double heads with an average of 19.83%, 12.75%, 6.42%, and 2,25% respectively can determine abnormalities of the sperm. To conduct the artificial insemination, drone sperm should always be available. For this reason sperm should be preserved in an optimal condition storage temperature (5°C, 27 °c and 37°C) and glucose concentration (0%, 0,3%, 0,6%, and 0,9%) containing in the dilution media. This condition can be affecting the sperm motility. The highest motility is achieved when the sperm is kept in 0,9% glucose concentration and storage in 5 °C temperature. In this condition 0.19x106 sperm/ml can survive up to 36 hours. Moreover, the higher concentration of sperm motility up to 36 hours is achieved when sperm is kept in 5 Celsius temperature with different leves of glucose in dilution media. However statistically interaction between glucose concentration and temperature levels does not give significant affects
Hemagglutination activities and phenotypic expressions of fifty-five S. agalactiae isolates consisted of 19 standard and 36 field isolates from subclinical mastitis cattle were observed. Five (eighteen); 3 (4); 2 (7); 1 (2); and 5 (11) isolates (numbers in brackets indicated field isolates) were able to hemagglutinate cattle, horse, sheep, chicken, and human erythrocytes, respectively. The distribution of hemagglutination pattern was discissed in this paper.
No full textHemagglutination activities and phenotypic expressions of fifty-five S. agalactiae isolates consisted of 19 standard and 36 field isolates from subclinical mastitis cattle were observed. Five (eighteen); 3 (4); 2 (7); 1 (2); and 5 (11) isolates (numbers in brackets indicated field isolates) were able to hemagglutinate cattle, horse, sheep, chicken, and human erythrocytes, respectively. The distribution of hemagglutination pattern was discissed in this paper
Blood Urea Nitrogen Constituent of Pregant Ewes
Full text linkThe research on blood urea nitrogen constituent of pregnant ewes has been carried out at the Departement of Physiology and Pharmacology, Faculty of Veterinary Medicine. Bogor Agricultural University. Ten ewes with about the same body weight and also about the same times of parturation were used in this experiment. PGF-, injection were used to sinchronize oestrous of those ewes. Blood were collected twice a month from the jugular vein and itsmetabolite constituent were analyzed. using the procedure as described in the kit.This result showed that the concentration of blood urea nitrogen in pregnant ewes was higher than in nonpregnant (
Fasciolasis Pada Domba dan Kambing di Rumah Potong Hewan Kotamadya Bogor
Full text linkTelah dilakukan penelitian fasciolasis pada domba dan kambing yang dipotong & Rurnah Potong Hewan Kotamadya Bogor pada bulan November sarnpai Desember 1992. Sebanyak 192 ekor domba dan kambing telah diamati terhadap kejadian fasciolasis pada organ hatinya. Metode penelitian yang dilakukan adalah dengan melakukan pemeriksaan berdasarkan skoring derajat kerusakan pada organ hati dengan melihat gambaran makroskoplk dan mikroskopik.Hasil pemeriksaan rnakroskopik diperoleh 14,06% menunjukkan gambaran hati normal dan 85.94% terinfeksi yaitu 76,04% terinfeksi akut dan 9.88% terinfeksi kronis.Sedangkan gambaran mikroskopik organ hati yang terinfeksi secara akut adalah perdarahan. degenerasi sel hati, peradangan dan proliferasi buluh empedu. infiltrasi selradang. dan admya \u27globula leucocvte\u27 pada mukosa buluh empedu. Pada infeksi kronis tampak fokus-fokus radang granuloma, mineralisasi dan fibrosis.