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The Effect of Stage of Estrous Cycle on The Development of Bovine Embryo Matured and Fertilized in Vitro
The objective ofthis study is to examine the effect of stage of estrous cycle on in vitro development of bovine embryo matured and fertilized in vitro. Cow ovaries were collected from slaughter house and were kept in a physiological solution. Oocytes were aspirated using GI8 neddle connected to 10 mL syringe containing phosphate buffer saline (PBS). After being washed, oocytes were matured in vitro in Tissue Culture Medium (TCM) 199 a physiological in 5% CO2 incubator at 39°C for 20-22 hours. In vitro fertilization was done in BO (Brackett and Oliphant) solution for 8 hrs, using frozen semen. Embryos were further cultured in either TCM-199 supplemented with 5% cow superovulated serum or chemically defined-serum free medium. If ovaries were classified due to their estrous stages, i.e. luteal and follicular, both cleavage (fertilization) and development rates did not show any differences (p < 0,5) in both treatment. The cleavage rate of oocytes collected from the follicular and luteal stages were 75.0% and 75.6%. respectively even though was lower if compared to that withaout classification (83.5 %). The development rate were 20,0% and 23,0%, higher, compare to that without classification, i.e. 13.0%. Nevertheless, if chemically defined-serum free medium was used, the two treatments showed differences (p < 0,5) in both cleavage and development rates. The ratio between luteal and follicular stages were 68,0% : 80,0% and 53,6%: 65,7% for cleavage and development rates, respectively
Infeksi Dirofilaria pada Anjing Kajian Patologik
Tiga ekor anjing yang terinfeksi secara alami oleh Dirofilaria immitis (Di) menunjukkan perubahan patologik sebagai berikut : ditemukan cacing dewasa pada ventrikel dan dilatasi jantung kanan, pneumonia, limfadenesis, splenitis, periphenitis dan penebalan pembuluh darah pada hati, nefritis interstisialis dan degenerasi organ parenkim, serta mikrofilaria (Mf) di dalam paru-paru, limfoglandula dan hat
Nilai Hematologi Rusa Sambar (Cervus unicolor) yang ditangkarkan
Nine tame young (10-14 monts of age) housed sambar, consided of 5 stags and 4 hinds, were blood sampled in May 1992 (late autum) and september 1992 (early spring) using physical restraint without sedation. In May 1992, four semidomestical adult sambar stags in hard antler; managed at pasture were sedated using a mixture of xylazine hydrochlorine (Rompun, Bayer, NZ) and fentanyl citrate-azaperone (Fentas, Ethnor Pty.Ltd.Nort Ryde, Australia), at a ratio of 3:1. administrated by traquillizer dart (Paxarms Ltd, Timaru, NZ) and were blood sampled. All samples were analyzed for standart haematological analysis (Hill et al.1992)
The Study on Aflatoxin Accumulation in Visceral Organs of Chicken Received Corticosteroid and Antibiotic
Seratus lima puluh ekor ayam petelur jantan digunakan dalam penelitian ini. Ayam-ayam tersebut dibagi ke empat kelompok besar yang terdiri dari Kelompok pertama adalah kelompok yang menerima aflatoksin dan antibiotik (45 ekor). Kelompok kedua adalah kelompok yang menerima aflatoksin dan kartikosteroid (45 ekor), Kelompok ke tiga adalah kelompok yang menerima afklatoksin saja (45 ekor) dan kelompok ke empat adalah kelompok kontrol. Masing-masing kelompok besar dibagi lagi menjadi tiga kelompok kecil dan tiap kelompok yang berbeda memperoleh aflatoksin dengan dosis 150, 250, dan 350 ppb di dalam media tepung beras melalui pakan. Pengamatan dilakukan terhadap bobot organ jeroan, perubahan patologi anotomi dan kandungan aflatoksin dalam masing-masing organ jeroan. Bobot organ kelompok yang menerima aflatoksinlebih berat dibandingkan yang tidak dan kelompok yang menerima obat antibiotika dan kortikosteroid lebih ringan dibandingkan yang tidak. Terjadi perubahan patologi dari organ hati, limpa dan bursa Fabrisius pada semua kelompok yang mendapatkan aflatoksin. Kandunagn aflatoksin di organ hati meningkat dan peningkatan pada kelompok yang mendapatkan antibiotika dan kortikosteroid lebih rendah dibandingkan kelompok yang hanya menerima aflatoksin. Kandungan aflatoksin di organ limpa dan busra Fabrisius menurun dan penurunan lebih cepat pada kelompok yang mendapatkan antibiotika dan kartikosteroid
Prevalence of Salmonella sp. on Hatched Failure of Eggs and One Week\u27s Duckling at the Hatchery Center for Alabio Duck in the District of Hulu Sungai Utara South Kalimantan
Six hundred and sixty five eggs of hatched failure and 150 of ducklings, from the hatchery of Alabio duck at the District of Hulu Sungai Utara (HSU), South Kalimantan, were examined for Salmonella sp. The results showed that 45 samples of eggs (6.8 %) and 18 samples of ducklings were positive to Salmonella sp (12 %). The serotipe of Salmonella sp. from eggs samples were 17 isolates of S. typhimurium (2.6 %),26 isolates of S. sentenberg (3.9 %), 1 isolate of S. hadar (0.2 %) and 1 isolate of S. virchow (0.2 %); and from duckling samples there are 5 isolates of S. typhimurium (3.3 %), 4 isolates of S. senftenberg (2.7 %), 5 isolates of S. hadar (3,3 %) and 4 isolates of S. virchow (2,7 %).Key words: Salmonella sp, hatchery, Alabio duc
Throughout the developing world, working animals are still vital important power bases of the small farmers\u27 systems of production and transportation. With economic development, there is a growing interest in the animals for pleasure purposes, not only for aesthetic value but also for sport requiring speed and stamina for racing and other physical performances. An understanding of the physiology of exercise of animals is therefore needed including practical application such as to evaluate cardiorespiratory fitness and information on training for improvement in health related fitness and performance. Under the existing local condition, success have been reached to record heart rate of animals telemetrically over extended periods, and to measure longterm body energy expenditures of large animals (buffalo) involving energy balance approaches and ill vivo body composition measurements by the water displacement method. These measurements enable the development of a modest practical fitness guideline for exercising swamp/ water buffalo, e.g. V02 = (0.1 X + 3.4) ml/min/kg BW for walking, and V02 =(0.2 X + 3.4) ml/min/kg BW for trot at 100-250 m/min speed. This value at a given speed offers a measure of running economy. The value of the oxygen pulse as an index of fitness is presented, above 0.05 is regarded athletic, while lower than 0.05 is the opposite. For the female buffalo the value is 0.066 at rest but decreases with exercise of pulling a load due to moving the body with a slower speed. Male buffalo has a higher oxygen pulse, 0.094 at rest and increases with exercise. Training seems to improve the oxygen pulse. These data enable the calculation of the heart\u27s stroke volume and the O2 debt, which are other parameters of fitness. The field of exercise physiology of athletic animals in Indonesia should be explored. The acquisition of a treadmill, blood gas analysis and ergocardiorespirometry equipment for large athletic animals would be an advantage.
Throughout the developing world, working animals are still vital important power bases of the small farmers' systems of production and transportation. With economic development, there is a growing interest in the animals for pleasure purposes, not only for aesthetic value but also for sport requiring speed and stamina for racing and other physical performances. An understanding of the physiology of exercise of animals is therefore needed including practical application such as to evaluate cardiorespiratory fitness and information on training for improvement in health related fitness and performance. Under the existing local condition, success have been reached to record heart rate of animals telemetrically over extended periods, and to measure longterm body energy expenditures of large animals (buffalo) involving energy balance approaches and ill vivo body composition measurements by the water displacement method. These measurements enable the development of a modest practical fitness guideline for exercising swamp/ water buffalo, e.g. V02 = (0.1 X + 3.4) ml/min/kg BW for walking, and V02 =(0.2 X + 3.4) ml/min/kg BW for trot at 100-250 m/min speed. This value at a given speed offers a measure of running economy. The value of the oxygen pulse as an index of fitness is presented, above 0.05 is regarded athletic, while lower than 0.05 is the opposite. For the female buffalo the value is 0.066 at rest but decreases with exercise of pulling a load due to moving the body with a slower speed. Male buffalo has a higher oxygen pulse, 0.094 at rest and increases with exercise. Training seems to improve the oxygen pulse. These data enable the calculation of the heart's stroke volume and the O2 debt, which are other parameters of fitness. The field of exercise physiology of athletic animals in Indonesia should be explored. The acquisition of a treadmill, blood gas analysis and ergocardiorespirometry equipment for large athletic animals would be an advantage
Percobaan ini dilaksanakan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya Malang dan Balai Inseminasi Buatan Singosari pada bulan Agustus sampai Desember 1998. Tujuan penelitian ini adalah untuk mencari metode pembekuan spermatozoa hasil seleksi jenis kelamin dengan menggunakan filtrasi sephadex G-200. Percobaan ini terdiri atas 10 kali ulangan dengan dua perlakuan yaitu krioprotektan ekstraseluler TCM- 199+ 10% serum+ 14% kuning telur dan tris aminomethan- kuning telur. Parameter yang diukur meliputi persentase motilitas, hidup, kapasitasi dan reaksi akrosom. Media TCM-199 kuning telur lebih dapat mempertahankan mutu semen dibandingkan dengan tris aminomethan kuning telur, dan proses pembekuan meningkatkan kapasitasi dan reaksi akrosom.
Percobaan ini dilaksanakan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya Malang dan Balai Inseminasi Buatan Singosari pada bulan Agustus sampai Desember 1998. Tujuan penelitian ini adalah untuk mencari metode pembekuan spermatozoa hasil seleksi jenis kelamin dengan menggunakan filtrasi sephadex G-200. Percobaan ini terdiri atas 10 kali ulangan dengan dua perlakuan yaitu krioprotektan ekstraseluler TCM- 199+ 10% serum+ 14% kuning telur dan tris aminomethan- kuning telur. Parameter yang diukur meliputi persentase motilitas, hidup, kapasitasi dan reaksi akrosom. Media TCM-199 kuning telur lebih dapat mempertahankan mutu semen dibandingkan dengan tris aminomethan kuning telur, dan proses pembekuan meningkatkan kapasitasi dan reaksi akrosom
The aims of this experiment were to study the physical and chemical properties of meat of Brahman Cross, Angus and Murray Grey cattle. Forty cattle consist of Brahman Cross, Angus and Murray Grey were used in this study. All animals were kept in feedlot with ad libitum ration and water. Data were analyzed by using covariance analysis. Result shows that breeds were significantly influenced of physical and chemical properties of meat. The Murray Grey meat was tenderness (P
The aims of this experiment were to study the physical and chemical properties of meat of Brahman Cross, Angus and Murray Grey cattle. Forty cattle consist of Brahman Cross, Angus and Murray Grey were used in this study. All animals were kept in feedlot with ad libitum ration and water. Data were analyzed by using covariance analysis. Result shows that breeds were significantly influenced of physical and chemical properties of meat. The Murray Grey meat was tenderness (P<0,05) than Angus and Brahman Cross. The meat of Brahman Cross was less fat content (P<0,05) than Angus dan Murray Grey