Jurnal Biodjati
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Fish Community Structure in The Coastal Mangrove Ecosystem of Cemara Kulon Village Indramayu Regency, Indonesia
Get PDF The mangrove ecosystem of Cemara Kulon Village has the potential for fishery resources which has decreased in line with various anthropogenic activities. This study aims to determine the composition of fish and analyze the status of the fish community structure and its relation to the physical-chemical aspects of the waters in the mangrove ecosystem of Cemara Kulon Village. This research was conducted over a period of three months  in the mangrove ecosystem of Cemara Kulon Village. The method used in this research was  descriptive quantitative purposive and composite sampling at 3 research stations. Parameters observed in this study were relative abundance, diversity, uniformity, dominance, and the relationship between community structure and aquatic physico-chemical parameters. During the study, 508 fish were caught, consisting of 20 species from 16 families. The value of diversity was in the moderate category with a ranged of 1.87-2.50; the uniformity value was in the moderate category with a ranged of 0.41-0.46 and the dominance value was in the low category with a ranged of 0.11-0.22.   Based on the results of the Canonical Correspondence Analysis (CCA) revealed that fish diversity was affected by light transparency and DO, the uniformity of fish was  affected by temperature, and the dominance of fish was affected by ammonia. The fish community structure was categorized as unstable which was influenced by water quality
Physiological and Anatomical Responses of Red onion (Allium cepa L.) to Drought Stress after Biofertilizer Application
Get PDFRed onion (Allium cepa) is a plant that requires sufficient water and is susceptible to drought stress. The use of biofertilizer is employed because it contains microorganisms that enhance nutrient availability and assist plant growth under abiotic stress conditions. This study aims to analyze the physiological and anatomical responses of red onion bulbs following the application of biofertilizer under drought stress. The doses of biofertilizer used were 0; 10;15; and 20 L/ha with field capacity levels of 25; 50; 75 and 100%. The parameters tested were bulb diameter, reducing sugar content number of bulb layers, anatomy of root cortex thickness, anatomy of root metaxylem diam Red onion eter, and fresh weight of red onion roots. The method used in determining the level of reducing sugar is the Nelson-Somogyi method. The method used for preparing the red onion root specimens was the embedding method. The results showed that the largest red onion bulb diameter was obtained from treatment A1B2, without the addition of biofertilizer at 75% field capacity. Meanwhile, the highest reduced sugar content was found at a dose of 15 L/ha with 50% field capacit
Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli
Get PDFSapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli
Relation of Soil Physical Parameters and Dominant Vegetation with Infiltration Capacity in Latuppa Sub-Watershed Palopo Indonesia
Get PDFThe inflitration capacity is the soil’s ability to accommodate water that seeps into the soil, reducing surface runoff that will cause flooding, such as in the downstream area of Palopo city. This study aimed to determine the influence of dominant vegetation and soil physical characteristics on infiltration in the upstream area of the Latuppa Sub-Das, Mungkajang District, Palopo city. Data collection was carried out by direct observation in the [email protected] field in a purposive manner in 10 plots, which represent the condition of vegetation in the upstream area of the Latuppa watershed, and each plot consisted of 3 replications to observe infiltration and soil samples. The data were analyzed via the rational method, the Horton method, and vegetation index analysis. Additionally, the data were analyzed via simple linear regression and multiple regression analysis to determine the influence of the dominant vegetation and physical characteristics of the soil on the infiltration capacity. The highest infiltration capacity was found in plot 10, with an infiltration capacity of 107.5 mm/minute (6,450 mm/hour) and an average infiltration rate of 0.4 mm/minute (24 mm/hour). The dominant vegetation cover is Cananga odorata, with a vegetation index value of 78.41%. Plot 10 has a height of 362.79 masl, a gentle slope, a crumbly soil structure, a clay sand texture, a very high soil density and 2.10% organic matter. Based on observations and data analysis, the factors that influence the infiltration capacity in the upstream area of the Latuppa subwatershed are vegetation conditions, altitude, slope and physical properties of the soil
Diversity and Abundance of Soil Arthropods in Terrestrial Area of Situ Lengkong Panjalu, West Java, Indonesia
Get PDFSoil arthropods play an important role in nutrient cycling and maintenance of soil structure. Thus, their abundance and diverÂsity can indicate the biological quality of the soil. Due to different soil management practices, arthropods are also very sensitive to enÂvironmental changes. This study aims to analyze the abundance and diversity of soil arthropods and the environmental factors that supÂport the abundance of soil arthropods in terrestrial of Situ Lengkong Panjalu, West Java, Indonesia. The methods used to obtain samples of soil arthropods are pitfall trap. Soil arthropod data were analyzed to determine the Shannon diversity index (H\u27), Margalef richness index (R), and Evenness index (E). The effect of abiotic environmental facÂtors was analyzed using Principal Component Analysis (PCA). A total of 1263 arthropods were found in Situ Lengkong Panjalu, belonging to 11 orders, 24 families, and 32 morphospecies. The current work determined the scores of H\u27= 2.08, R = 3,08, and E = 0.72 of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. Results revealed that humidity is one such environmental parameter affecting the presence of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. This research can be used as a guide in validating and conservation of the habitat of soil arthropod species on West Jav
In Vitro Evaluation of Green Tea Extract\u27s Influence on Local Sheep
Get PDFIn recent years, the exploration of natural supplements such as green tea extract and their potential benefits in livestock nutrition has gained considerable attention among researchers and agriculturalists. Green tea extract contains a high concentration of bioactive substances, such as polyphenols and catechins. This study aimed to explore the effects of varying doses of green tea extract on rumen fermentation parameters through an in vitro trial using sheep rumen liquids. A completely randomized experimental design was utilized to test the effect of 4 different doses of green tea extract treatments (0, 140, 280, and 560 mg/kg) on in vitro Hohenheim gas test method. The current study shows no significant effect of administering green tea extract at various doses on total gas production, total gas production per in vitro dry matter and organic matter degradability, volatile fatty acid per in vitro dry matter and organic matter degradability, methane production per inv vitro dry matter and organic matter degradability of green tea extract. Although no significant effects were observed on the investigated parameters, this study provides important insights in the understanding of the potential use of green tea extract in modulating fermentation processes in the ruminant’s stomach. In conclusion, the findings of this study underscore the impact of GTE on gas production regulation, highlighting its potential implications for gastrointestinal healt
Characterization of Orexin Gene in Tilapia (Oreochromis niloticus):Regulator Feeding Appetite, and Correlation with Reproductive Factors
Get PDFThe mechanism of energy allocation related to increased appetite and feed intake in fish involves several neuropeptides encoded by specific genes. Orexin (OX) is a peptide secreted in the pars tuberalis of the hypophysis that acts as a stimulator in appetite increase (orexigenic factor). However, the expression of different orexigenic factors varies among vertebrate species, reflecting their unique types and lineages. Therefore, this study aimed to identify genes encoding appetite in Tilapia. RNA isolation, complementary Deoxyribose Nucleic Acid (cDNA) cloning, and DNA amplification were performed from brain samples of gonadally mature Tilapia. The PCR products were subsequently sent to Macrogen.Inc for sequencing. The amplification results of Orexin with β-Actin (positive control) observed using agarose gel electrophoresis showed that the size of the nucleotide base product of each gene was 196 bp and 197 bp. Confirmation of sequencing results carried out using the Basic Local Alignment Search Tool (BLAST) method - National Center for Biotechnology Information (NCBI) for Orexin and β-Actin were 97% and 100%. Based on these results, it can be concluded that each target gene isolated from the tilapia brain showed homology/similarity with the sequence available in the NCBI databas
Propagation of Cardamom (Amomum compactum) Using Vitamin B1, Indole Butyric Acid and Their Combinations Ex Vitro
Get PDFCardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enÂhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters
The Growth of Oyster Mushroom on Ramie Chip Waste-Derived Media was Accelerated by Rice-washed Water
Get PDFRice-washed water is wastewater resulted from the rice cleaning process that is rich in nutrients. The use of ramie chip waste can be considered a potential substrate due to its lignocellulosic nature, similar to other wastes studied. This study aimed to examine the effect of rice-washed water on the mycelium and pinhead growth of white oyster mushrooms grown in the ramie chip waste as the growing medium. The experiment was conducted for three months using a completely randomized design with four rice-washed water treatments and six replications. The rice-washed water concentration treatments were 0% (K0), 25% (K1), 50% (K2), and 100% (K3), which were added to the ramie chip waste as the growing medium for white oyster mushroom. The time of mycelium propagation velocity, the time the first pinhead appearance, the number of mushroom bodies, and the largest cap diameter were observed. The results showed that ramie chip waste-derived media was suitable to cultivate white oyster mushrooms. Adding 100% rice-washed water to the media significantly affected the speed of mycelium propagation (16.51 days) compared to control (30 days) and the appearance of the first pinhead (31.5 days) compared to control (38.5 days) as well as the number of fruiting bodies of mushroom (8.8) compared to control (6.2). However, the rice-washed water application did not significantly affect the diameter of the largest mushroom cap
Ganoderma Diversity and Its Interactions with Hosts and Neighbouring Insects in Gunung Walat Educational Forest
Get PDFGanoderma spp. is a genus of macrofungi in the Basidiomycota, comprising both saprophytic and phytopathogenic species. Ganoderma spp. prefers woody substrates and can attack various trees in the forest. While it often acts as a phytopathogenic fungus, it also serves an ecological role for surrounding insects, providing food and shelter. This study aims to determine the interactions and preferences of Ganoderma spp. with its hosts and to observe the interactions between Ganoderma spp. and surrounding insects. Research on these interactions was conducted through scan sampling exploration in the Gunung Walat Educational Forest. Identification of fungi, trees, and insects was based on morphological characteristics. A total of 155 fruiting bodies of Ganoderma were found. Three species of Ganoderma were successfully collected and described morphologically, both macroscopically and microscopically. Ganoderma spp. were found infecting Agathis sp., Schima wallichii, and Pinus merkusii trees. The fruiting bodies of Ganoderma spp. interacted with insects such as larvae, Trichomyrmex destructor ants, and Eumorphus marginatus beetles, which used the fruiting bodies as breeding and shelter site