Jurnal Biodjati
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Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant
Get PDFA commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study.Â
Isolation and Characterization of Indole-3-Acetic Acid Producing Bacteria from Cow Urine
Get PDFÂ Â Cow urine contains urea as nitrogen source, therefore it can be expected to isolate the beneficial bacteria for plants, for example indole-3-acetic acid (IAA) or auxin producing bacteria. The objective of research was to obtain IAA producing bacteria from cow urine, to characterize bacterial isolate, and to measure its ability to stimulate the growth of green bean seedlings (Vigna radiata). The methods used in this study were collecting urine from cow cattle, obtaining IAA-pro-ducing bacteria from urine, measuring IAA using Salkowski method, and applying selected bacterial supernatants on green bean seedling plants. The number of IAA producing bacteria that was successfully purified was 18 isolates. There are five isolates, namely US 5, BS1, BS 2, BS 4 and BS 5 which have the ability to solubelize phosphate on Pikovskaya agar. The five isolates were also able to fix free nitro-gen on N Free media and did not show hypersensitivity on tobacco leaves. The results of the growth of isolates in blood agar showed positive for US 5 and BS 2 as beta hemolysin producers. Further-more, isolate BS 4 was chosen to produce exogenous IAA quantita-tively. Isolate BS 4 produced IAA 6.364 ppm at the 45 h incubation at stationary phase. The use of BS 4 supernatant on green bean seed-lings showed an effect on plant height and lateral root length better than control (without treatment) on 6 days after planting. Morpho-logical characteristic of isolate BS 4 was rod shape, Gram positive, endospore producing, aerobic, and had similarity with genus Bacillu
Effect of Pectin Biodegradation with Aspergillus niger on Total Flavonoid Content of Citrus limon L.)
Get PDFLemon peel and leaf  as well as lower grade of lemon contain a considerable amount of flavonoid which can be extracted to produce a valuable product. The presence of pectin in lemon hinders the extraction of flavonoid compound. This research was conducted to examine the effect of pectin biodegradation of lemon leaf, peel, and lower grade fruit using solid-state fermentation towards the yield of pectin, crude flavonoid, and total flavonoid content. Fermentation was carried out with the help of Aspergillus niger at 30℃ for 3, 5, 7, and 9 days of cultivation time. Pectin was extracted from the fermentation broth using citric acid. Crude flavonoid was extracted using maceration and stirred about 500 rpm at 50℃ for 40 minutes. Total flavonoid content was analyzed using a spectrophotometer. The optimum cultivation time of Aspergillus niger with leaf and peel was 9 days with a pectin yield from lemon leaf was 0.43% dry weight and the pectin yield from lemon peel was 0.04% dry weight. As for the lower grade fruit, a cultivation time for 7 days showed an optimal result with a pectin yield of 0.08% dry weight. The total flavonoid content in the leaf increased up to 94.3% (1.06 mg of the quercetin equivalents per g of substrate) whereas the total flavonoid content in the peel and lower grade fruit increased up to 42% (0.12 mg of the quercetin equivalents per g of substrate) and 48% (0.021 mg of the quercetin equivalents per g of substrate), respectively. These results highlighted the potential valorization of Citrus limon L. as an alternative source for producing flavonoid in food and pharmaceutical industry. Â
The Growth Optimization of Pak Choy (Brassica rapa L. var. chinensis) in Household-Scale Aquaponics System
Get PDFThe growth of Pak Choy (Brassica rapa var. chinensis) using nutrients from catfish “Sangkuriang†(Clarias gariepinus) wastes was investigated in the household scale aquaponics systems by comparing the growth parameters value among the grow beds. The growth parameter of Pak Choy was fresh weight, leaf number, and leaf area. Meanwhile, catfish growth parameters were body weight and length, and feed conversion ratio (FCR). The experiments were arranged in a Completely Randomized Design (CRD) with 4 treatments and 3 replications. The data were analyzed using Analysis of Variance (Anova) followed by Duncan Multiple Range Test (DMRT) at 95% level of significant The result of the study showed that there were not significantly different in the growth parameter of Pak Choy among the grow beds in the vertical system. However, those in grow beds “Ćwere significantly higher than the rest of the grow beds in the horizontal aquaponics system. In general, all the growth parameter of Pak Choy in the vertical system was higher than in the horizontal one, although, it was not statistically significantly different. Nevertheless, the horizontal aquaponics system gave better results in the body weight and FCR of catfish.Â
Genetic Relationship of the Transgenic and Non-Transgenic Mutiara Catfish with their Hybrids Sangkuriang Catfish
Get PDFThe male broodstock of Transgenic Mutiara catfish (P) (containing Clarias gariepinus Growth Hormone, CgGH) is a fish who show fast growth. Propagation of the Transgenic Mutiara catfish F1 and F2 progeny involves the crossing of broodstock P with Non Transgenic Mutiara catfish. New genetic variations (polymorphisms) can be generated from crosses broodstock P and F1 which lead to the fast growth inheritance of transgenic fish. Phylogenetic relationship analysis showed distance and genetic variation in broodstock P, F1, F2 and Sangkuriang catfish (as controls) using the RAPD-PCR method (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) as the research purposes. The results showed that OPA-03 (5\u27-AGTCAGCCAC-3 \u27) primer was selective for determining phylogenetic among catfish samples. The closest genetic similarity index originates from the female broodstock of Non Transgenic Mutiara catfish (P) (93%), while the farthest originates from the male broodstock of Mutiara Transgenic catfish (P) (51%) with F1 dan F2. Closely related broodstock catfish is not recommended to be crossed
Etnobotanical Study on Banana in Karangwangi Village, Cianjur District, West Java
Get PDF  Banana has been known as one of fruits that has an important function in the rural areas. Bananas have been traditionally planted by rural farmers in the homegarden, garden and mixed-garden in West Java. However, study on bananas in Karangwangi village,Cianjur has not been carried out. Aim of study was to explore varieties (landraces) of bananas, source of local knowledge on bananas, utilization of ba-nanas and diseases of bananas. Method used in this study was quali-tative with ethnobotanical approach and some techniques, including observation and semi-structure interview were applied in this study. The result of study showed that, it was recorded 13 variations of ba-nana; main source of local knowledge on bananas from the parent and friends; utilization of bananas, including consumption of ripe fruit, made of “sale†and some banana organs, including leaves, “jantung†(male flower), pseudostem, â€bonggol†(base of pseudostem) and roots of bananas were usually used by people. Main diseases of banana was known by local people as “Pireus†(virus). We recommended more intensive study on bananas must be carried on for near future
Molecular Characterization Reveals Genetic Differences Between Wild and Captive Populations of Mandiangin Giant Gourami (Osphronemus goramy)
Get PDF  Captive population of Mandiangin giant gourami (Osphronemus goramy Lac.) is a new strain developed from wild pop-ulations in Riam Kanan Dam. No much is known about the genetic constituent of this strain compared to the parental population. This re-search was done to obtain information whether genetic alteration has occurred in captive population compared to their wild parental pop-ulation. Wild population was caught from Riam Kanan Dam, while captive population was collected from Balai Perikanan Budidaya Air Tawar (BPBAT) “Freshwater Aquaculture Centre†Mandiangin, South Kalimantan. The captive population of Batanghari strain from BPBAT Sungai Gelam, Jambi was also analyzed. Molecular assess-ment was done using CO1 PCR-RFLP which was developed by digest-ing the amplicon of the CO1 gene from all populations using two re-striction enzymes, namely HindIII and TaqI and resulted CO1-HindIII and CO1-TaqI RFLP markers. All tested populations showed uniform patterns of those CO1-RFLP markers but with different fragment sizes among populations. This means all resulted RFLP markers were mono-morphic in each populations but polymorphic among giant gourami populations. In other words, no genetic diversity within population but substantial genetic differences was observed between wild and cap-tive Mandiangin populations. This proved that genetic alteration has occurred in Mandiangin captive populations compared to the wild pa-rental giant gourami population. In conclusion, captive and wild pop-ulations of Mandiangin giant gourami were genetically different. This result is vital as scientific base for future development of inland fish-eries, especially for breeding strategy of Mandiangin giant gourami.Â
Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa
No full text  Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mare’s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations.Â
Antioxidant, Antibacterial Activity and GC-MS Analysis of Extract of Giant Forest Ant Dinomyrmex gigas (Latreille, 1802)
Get PDF Giant forest ant Dinomyrmex gigas is one of the largest ants species in the world, native to the rain forests of Southeast Asia. It is known that ants have glands that produce chemical compounds that inhibit the growth of microbes. Therefore, it is necessary to deter-mine the antioxidant and antibacterial activities as well as identify the chemical compounds of D. gigas extract. D. gigas was extracted successively with n-hexane, ethanol and methanol. The antioxidant activity was evaluated by determination of the half-maximal inhibi-tory concentration (IC50) values while the antibacterial activities of the extracts were determined by measuring the minimum inhibitory concentration (MIC). The results exhibited that the IC50 values of n-hexane, ethanolic and methanolic extracts were 336.18±0.0984, 89.16±0.0219 and 90.72±0.0894 μg/mL respectively. The ethanolic extract exhibited the highest AAI value (0.34) followed by metha-nolic extract (0.33) and n-hexane extract (0.09). Based on AAI val-ues, the extracts were classified as moderate antioxidants. The best MIC values were 625 μg/mL for both ethanolic and methanolic ex-tracts against S. aureus, while MIC values of all extracts against E. coli were >625 μg/mL. Based on MIC values, all of the extracts presented weak activity against both S. aureus and E.coli. The GC-MS analysis showed that there are up to 30 compounds construct-ed of the ethanolic extract. Three major compounds are ethyl oleate  (29.78%), n-hexadecanoic acid (17.54%) and oleic acid (10.65%).Â
Growth of Slipper Orchid Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer during Acclimatization Stage
Get PDFÂ Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer known as slipper orchid is an endemic terrestrial orchid from Indonesia. This orchid is threatened with extinction and listed under CITES Appendix I. In-vitro propagation of P. javanicum has been done in Bali Botanic Garden (BBG) for last six years but acclimatization stage has not given satisfied result. The purpose of this study was to know the ef-fect of liquid fertilizer on vegetative growth of P. javanicum planlet during acclimatization stage. Beyonic StarTmik liquid fertilizer ap-plied by five different doses considered as treatment of 0, 10, 20, 30 and 40 mL. They were 10 planlets for each treatment considered as replication. In this research we observe vegetative growth of P. ja-vanicum by quantitative and qualitative parameter. Quantitative data were analysis by ANOVA. Result showed, high percentage of planlet survival was observed of 98% and liquid fertilizer dose for 30 mL gave the optimal vegetative growth of P. javanicum planlet with aver-age height of 2.2 cm and leaves number of 13 pieces. There were no differences on qualitative parameter in all treatment that all leaves color was dark green and leaves tessellation appearance was obviousÂ