Jurnal Biodjati
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The Role of Endothelial Microparticle in Coronary Heart Disease as The Complications of Diabetes Mellitus
Get PDF  Coronary heart disease (CHD) is caused by obstruction of coronary blood flow due to endothelial dysfunction triggered by various genetic and non-genetic risk factors such as hyperlipidemia, hypertension, hyperglycemia and obesity. Endothelial cell activation due to hyperglycaemia in diabetes mellitus induces production of pro-inflammatory factors that damage the cell membrane triggering the formation of membrane particles called microparticles. Endothe-lial microparticles contain proteins including endothelial nitric oxide synthase (eNOS) which plays a role in the production of nitric oxide (NO). To determine the role of microparticles in the occurrence of coro-nary heart disease in diabetes mellitus due to endothelial dysfunction, a study was conducted by comparing the levels of eNOS and NO in DM patients who had CHD with DM patients who had no CHD. Blood samples from 20 DM patients who had CHD and 20 DM patients who had no CHD of the outpatients in Cardiology Department and Inter-nal Medicine department of regional public hospital were included in this study. All patients were fulfilled inclusion and exclusion criteria and diagnosed by the appropriate specialist. The eNOS and NO lev-els were measured using the ELISA method. The results of this study show that eNOS levels in the group of DM patients who had CHD (21,292±12,415 ng/ml) were significantly lower (p <0.05) than those in the group of DM patients who had no CHD (29,721±11,952 ng/ml). Nitric oxide levels in DM patients who had CHD (0,053±0,021 nmol/ μl) were not statistically different to the levels in DM patients who had no CHD (0,047±0,032 nmol/μl). From the results of this study we concluded that endothelial microparticle protein eNOS plays a role in the occurrence of CHD due to the complications of diabetes mellitusÂ
Growth and Flavonoid Content of Gynura procumbens (Lour) Merr. in Different Soil Water Content
Get PDFGynura procumbens contains tannins, saponins, steroids, triterpenoids, essential oils and flavonoids which have bioactivity as a medicine for various diseases. One effort to obtain the quality of medicinal plants uses management of soil water content. The opti-mum of soil water content will support optimal growth of a plant. Whereas in conditions of low soil water content resulted in the induc-tion of the production of secondary metabolites as a self-defense sys-tem. This study aimed to determine the effect of soil moisture content on the growth and total content of plant flavonoids G. procumbens. This study was carried out experimentally using a one-factor com-plete randomized design (CRD) consisting of 4 treatments with five replications. The treatment used is the difference in soil water content of 40%, 60%, 80% and 100% field capacity. The data obtained in the form of quantitative data will be analyzed by One Way ANOVA Test. Measurement of the content of total flavonoid compounds was car-ried out using UV-Vis spectrophotometer analysis. The results of the study indicate the influence of soil moisture level on the growth and total content of G. procumbens. Parameter growth of fresh weight, dry weight, and root G. procumbens showed an increase in 40% soil water content. The flavonoid content showed the highest total flavo-noid content 18.884 mg/g in the 40% field capacity soil water contentÂ
Anatomical Structure of Sepal and Petal of Red Dragon Fruit (Hylocereus polyrhizus Britton & Rose) During Flower Development
Get PDFRed dragon fruit (Hylocereus polyrhizus Britton & Rose) is one type of cactus plant which is very potential as an ornamental plant and edible fruit. Flower is organ that play an important role in the process of breeding plants generatively. This reasearch aimed to study the anatomical structure of sepals and petals of red dragon fruit plants during flower development. The research stages included: sepals and petals sampling that held at various stages of flowering ; morphological observation (measurement length of sepals and petals); anatomical slides of sepals and petals cross section using the embedding method, anatomical observation and image capture of sepals and petals. The parameters observed were bud size, sepal length, petal length, sepal thickness, petal thickness, and tissue description composed. The results of this study indicated that buds have an increased development pattern. The increase in bud size is directly proportional to the stage of the bud. Sepal and petal are composed of epidermal tissue which form papillae, cryptophore stomata, secretory parenchyma space containing mucus, and tissues transport system is closed collateral
The Diversity and Diameter Growth Rate of Sprouting-Type Tree in Bukit Pinang-Pinang Forest Permanent Plots
Get PDFThe process of forest regeneration can take place within a certain period of time. Sprouting is a form of strategy for in adapting to its environment for success in regenerating. The Research on  the diversity and diameter growth rate of sprouting-type tree in Bukit Pinang-pinang forest permanent plots, West Sumatra was carried out from October 2012 to December 2016 in the forest of Bukit Pinang-Pinang, West Sumatra and Herbarium Department of Biology, Universitas Andalas, Padang. This research was conducted using a survey method by census. The parameters analyzed were plant species composition, Morishita Index, Mann-Whitney test, Regression and Correlation test as well as RDGR (Relative Diameter of Growth Rate). The result of experiment was found 40 species of trees sprouting type in forest permanent plot Bukit Pinang-Pinang. The pattern of sprouting type trees distribution in the study locations was found to have a clustered, random and uniform pattern. Relative Diameter Growth Rate (RDGR) of 40 sprouting types of trees in the Bukit Pinang-Pinang forest permanent plots ranged from 0.003 cm/cm/year to 0.0288 cm/cm/year. Altitude did not affect  RDGR of an individual tree sprouting type for 31 year
Prevalence and Intensity of Ectoparasites on Clarias gariepinus From Aquaculture Pond in Aceh Besar District, Indonesia
Get PDFInformation related to the prevalence and intensity of para-sites in an aquatic environment is crucial as preventive and responsive efforts to manage aquatic resources, especially for fish farming pur-poses. Up to now, information related to the prevalence and intensity of ectoparasites on catfish from aquaculture ponds in Aceh Besar Dis-trict is rare. This study aims to investigate the prevalence, intensity, and dominance of ectoparasites on catfish from aquaculture ponds in Aceh Besar district. The total of 200 catfish from four research stations at Sibreh, Kareung, Lambaro, and Limpok was observed in this study. The ectoparasites observation was focused on the skin, fins, and gill of the catfish. The observation parameter consists of prevalence, in-tensity, domination, length-weight relationship, and condition factor. Identification result showed that there were four types of ectoparasites infect the catfish in the aquaculture ponds in Aceh Besar District i.e Trichodina sp., Gyrodactylus sp., Dactylogyrus sp. and Ichthyopthyri-us multifiliis. Station 3 (Lambaro) had the highest prevalence and in-tensity, which were 64% and 5.87 ectoparasites/fish, respectively. In-fected catfish tended to have a lower weight growth than healthy catfish
Actinomycetes from the Soil of Chilli Plantation in Yogyakarta Showing an Antagonism to Fusarium oxysporum FU3
Get PDF Application of biocontrol agent is an environmental-ly-friendly method to overcome the Fusarium wilt in chilli due to Fusarium oxysporum. This research aimed to isolate actinomycetes antagonist to F. oxysporum FU3 as biocontrol agent from the soil of chilli plantation in Kulon Progo, Yogyakarta. The actinomycetes were isolated using SCA medium and followed by screening for anti-F. oxysporum FU3 on PDA. The filtrate from isolates which showing the highest inhibition zone was produced and tested against strain FU3. The best isolate was then characterized. There are four iso-lates of actinomycetes showing antagonistic activity against F. ox-ysporum (AK4, AK5, AK7, and AK9). The highest antagonistic ac-tivity was shown by AK5 filtrate with inhibitions on PDA and PDB are 41.71 ± 3.5 % and 81.77 ± 5.1 %, respectively. Further obser-vation showed that filtrate of AK5 caused abnormality and lysis on FU3 hyphae. From the results, we concluded that AK5 is a poten-tial biocontrol agent against F. oxysporum. Further studies to opti-mize the production and formulize the bio-fungicide are suggestedÂ
Community Structure of Fish and Environmental Characteristics in Cisadea River, West Java, Indonesia
Get PDF Environmental characteristics can influence the distribu-tion of animal communities including fish in aquatic habitats such as a river. The study of fish communities in Cisadea River aimed to learn the relation between the fish community structure and the environmental characteristics of the river itself. This research was conducted in Cisadea River, Cianjur Regency, West Java, Indone-sia during dry season from July 2012 until February 2013. Samples of fish were caught using active fishing gear (Electrofishing Gear, spreading nets, scoop, lift net) and passive fishing gear (trap). The results showed that the quality of the physical chemistry of Cisadea waters was still in the normal range that could support fish life. The fish community in Cisadea River consisted of 11 orders, 26 families, 35 genera and 48 species. During the study, Rhyacichthys aspro (19,85%) and Glyptothorax plathypogon (20,15%) were the domi-nant species caught. The diversity index value (H’) was 2.87 (mid-dle category) with the dominance value of 0.09 (low category) and evenness value of 0.74 (high category). Moreover, community simi-larities according to the presence of species made the LC1 and LC2 locations in one group while the LC3 locations in a separate group.Â
Vegetation Analysis of the Secondary Forest Area of ​​Mount Merapi National Park
No full text Mount Merapi National Park (MMNP) is a natural con-servation area with the main function of preserving the diversity of plants, animals and their ecosystems. In 2010, Mount Merapi suffered a large eruption which caused damage to the forest ecosystem inside. One of the locations that suffered severe damage was at Cangkrin-gan Resort. After the eruption, the affected vegetation began to grow again. The purpose of this study is to monitor and determine the di-versity of secondary forest plants in the MMNP area. The method used was a method of path and use a point-intercept transect as the sam-pling unit. The results showed that after the eruption, the Acacia de-curens dominated at each level starting from sapling, poles and trees. Based on the results of the study showed that the eruption of Mount Merapi that occurred in 2010 resulted in a change in vegetation struc-ture in the area with the Shannon-Weiner diversity index which is still low, amounting to 1.86 for seedling, 1.32 for the sapling and 0.22 for poles. At the tree level 100% is still dominated by Acacia decurens. Â
Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes
Get PDF An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C.Â
Nine Additional Cultivated Species of Asteraceae from Java
Get PDFAsteraceae is one of the largest families in the Angiosperm. It comprises of 24,000‒30,000 species which are grouped into 1,600‒1,700 genera and distributed in a cosmopolitan range. Based on Backer and Bakhuizen van den Brink’s 1965 works, they identified 227 species of Asteraceae in Java. Many of them are introduced species, both cultivated or naturalized. However, the cultivated species have not been recorded. The aim of this study is to provide information about new records of cultivated Asteraceae in Java. The observations were carried out in Bandung, Bogor, and Sumedang, during January to February 2019. Nine additional species were discovered; species belonging to genera Caputia (two species), Curio (two species), Kleinia (one species), Gymnanthemum (one species), Senecio (two species), and Tarlmounia (one species). These species were grouped into two tribes: Vernonieae and Senecioneae. This study indicates that information on Asteraceae of Java need to be updated