Jurnal Biodjati
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Species Density and Lead (Pb) Pollution in Mangrove Ecosystem, South Kalimantan
Its crucial to get information about lead (Pb) heavy metal pollution from mining and oil palm plantation on species density in mangrove ecosystem, to anticipate its impacts. This study aimed is to compare the types and densities of vegetation in mangrove ecosystems allegedly due to mining in Setarap village, Tanah Bumbu Regency and oil palm plantations in Kuala Tambangan Village, Tanah Laut Regency. We also analysis the condition of waters (TDS, pH and DO) and organic content in sediments to acquire data from the South Kalimantan mangrove ecosystems. The results showed there were four species of true mangroves (Avicennia alba, Acanthus ebracteatus, Nypa fruticans and Rhizophora apiculate) could live well in the environment affected by mining or oil palm plantations. The species density for trees was low (933 ind/ha) for mangroves affected by coal mines, while those affected by oil palm plantations had higher densities (1,067 ind/ha). pH value of waters in affected area by coal mining showed more acidic value (pH 5.76) especially at the back, while those by palm oil plantations are more acidic (pH 6) in the estuary. Organic matter content in sediments affected by coal mines was in the range of 0.61-6.59%, while those affected by oil palm plantations showed higher values (0.12-2.19%). Lead heavy metal content (Pb) in waters affected by coal mines was 0.031-0.056 mg/L, while the area affected by oil palm plantations was of higher value (0.110-0.128 mg/L). Lead (Pb) levels in sediments indicate higher values than waters, which reach 3.512-6.046 mg/Kg (affected by coal mines), and in areas affected by oil palm plantations reaching 6.658-6.66 mg/Kg. The general conclusion is that vegetation densities in areas affected by coal mines are lower than oil palm plantations. The level of lead  (Pb) pollution in the sediments is higher than in the waters
Effectiveness of Modified Traps for Protection Against Fruit Flies on Mango
Mango is one of the primary fruit export commodities in Indonesia. The productivity of mango can be increased by controlling the pest population, including fruit flies Bactrocera spp. Various efforts to control the population of fruit flies have been carried out, either by implementing traditional methods or by using synthetic pesticides. However, synthetic pesticides can cause pollution, and therefore we need to control the fruit flies’ population by using the most effective fruit fly trap mode. The study was conducted in the mango yield (monoculture) of Department of Agriculture in Majalengka. The traps in this study were modified Steiner traps with funnels, modified Steiner traps without funnels, bottle traps with funnels, bottle traps without funnels, and modified gypsy moth traps. Each trap was fed with 0.2 ml of Methyl eugenol dripped on a cotton roll and hung in the trap. The results showed that the number of fruit flies caught in each trap every week is significantly different. The highest number of fruit flies caught in each trap every week was achieved by bottle traps without funnels that caught 135 flies, while the lowest number of fruit flies caught was recorded by the gypsy moth traps with 16 flies. The transparent color of the bottles and a large number of holes effectively lured the fruit flies into the bottle traps without funnels
Vegetation Structure, Composition, and Soil Properties of Dry Land on Islands in Balikpapan Bay, East Kalimantan
   Some small islands in Balikpapan Bay consist of dry land which may have different vegetation structure and composition. Our study was the first to uncover vegetation conditions and soil proper-ties of dry land on small islands in Balikpapan Bay, which has never been conducted before. The research was carried out on Kalawasan and Karantina islands. Information on vegetation was obtained by applying a line transect method. Data on soil properties were col-lected by setting up 3 sampling plots in each transect of vegetation. Parameters used for analysing vegetation including relative densi-ty, relative frequency, relative dominance, Shannon-Wiener diversi-ty index, evenness index, and similarity index. Meanwhile, data on soils were analysed descriptively. Trees on Kalawasan island were comprised of 241 individuals belonging to 21 species. There were 61 individuals (22 species) of trees on Karantina island. The diver-sity index of trees on Kalawasan and Karantina island was 2.55 and 2.72, respectively. The tree evenness index was 0.84 on Kalawasan island and 0.88 on Karantina island. The index of similarity for trees between the two islands was 0.21. Furthermore, the soil character-istics on Kalawasan and Karantina islands showed similarities. In general, soil in Kalawasan and Karantina islands could be catego-rised as marginal soil which was common in East Kalimantan. Con-sidering the rapid loss of tropical forests in the mainland of Kali-mantan indicates that the small islands of Balikpapan Bay play an important role in future biodiversity conservation in East Kalimantan.Â
Microplastics in Pelagic and Demersal Fishes of Pantai Baron, Yogyakarta, Indonesia
Yogyakarta is the second-largest producer of plastic waste in Indonesia. Plastic waste in the waters can be degraded into microplastics that can enter the body of a fish. This study aimed to determine the presence of microplastics in the digestive tract of several species of fish in Pantai Baron, Yogyakarta, Indonesia. This research was an exploratory research conducted in April-September 2019 at Pantai Baron, Yogyakarta. Two species of each pelagic and demersal fish samples were taken by buying fish caught by fishermen. The digestive tract of fish was extracted with 10% KOH, filtered, then visual identification. Microplastic types were estimated by FT-IR spectrometry. The amount of microplastic of all fishes was done by Kruskal-Wallis test while the number of microplastics of the two categories of fish was compared with the Mann-Whitney test. A correlation analysis was carried out between the length of the fish, the weight of the digestive tract, and the number of microplastics found. Microplastic shape, color, size, and type data were analyzed descriptively. A total of 78 of the 80 (97.50%) fish from four species studied contained microplastics with a total number as many as 3,651 (mean 45.60 ± 44.31 microplastic/individual). About 100% of pelagic fish samples contained microplastics, while only 95% of demersal fish samples contained microplastics. Mann-Whitney test results showed the number of microplastic/ individual was significantly different between pelagic and demersal fish (p <0.05). The most dominant shape of microplastic was fiber (53.14%), film (36.97%) and fragments (9.89%). The type of polymers detected was polyamide. The results showed that pelagic fish swallowed more microplastics than demersal fish did because of the microplastic nature that is lightweight and floats. Microplastic characteristic data can be used to estimate the main source of microplastic pollution in Pantai Baron so it can be managed appropriately
Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice
SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic markers on five cross-progeny which have shown the same band pattern as the parents and tolerant control on the use of 9 SSR primers recorded as follows: RM257 2 lines, RM214 5 lines, RM247 5 lines, RM205 lines, RM490 13 lines, RM262 5 lines, RM569 7 lines, RM271 4 lines, and RM19 6 lines. The selected SSR primers linked to Al tolerance in this research can be used as a reference for molecular breeding strategies to develop new Al tolerance rice varieties in dryland conditions
The Effect of Coffee Fruit Skin Extract on Sperm Characteristics And Testicular of Mice With Ethanol-Induced
Coffee fruit skin contains antioxidant compounds that can repair damaged tissues, especially those of reproductive or-gans. This study was designed to assess to what extent the metha-nolic extract of coffee fruit skin affected sperm characteristics and testicular histology in mice receiving 15% ethanol for 15 days. It used 25 male mice aged four months and employed a completely randomized design with two controls (K) and three treatments (P), namely K1 (without ethanol), K2 (15% ethanol), P1 (15% ethanol and the methanolic extract of coffee fruit skin, or MECS, at 125 mg/ kg BW), P2 (15% ethanol and MECS 250 mg/kg BW), and P3 (15% ethanol and MECS 500 mg/kg BW). These treatments were administered orally with a 1 mL disposable syringe for 15 days. The parameters observed were sperm characteristics (viability, morphology, and sperm count) and the microscopic structure of the testicles (lu-men area, diameter, and area of seminiferous tubules). These data were then analyzed using the one-way ANOVA, continued with the LSD and Duncan’s tests (P <0.05). The results showed that P2 (250 mg/kg BW) substantially improved sperm count (86.5x105±1.73 cells/ mL), sperm viability (87.26±0.05%), and the proportion of normal sperm morphology (93.33±0.004%) of mice with 15% ethanol-in-duced sperm damages. Testicular histology also confirmed improve-ments in spermatogenic cells, as evident from the lumen, diameter, and area seminiferous tubules after receiving P1 and P2. In conclu-sion, the optimum dose of the coffee skin extract for improving sperm quality and microscopic structures of mice testicles is 250 mg/kg BW.Â
Influence of Mates Virginity on Black Soldier Fly, Hermetia illucens L. (Diptera: stratiomyidae) Mating Performance
   Hermetia illucens (L.), the black soldier fly (BSF), has raised attention due to its potential in solving various organic waste prob-lems and the benefits of the prepupa biomass as an alternative highly nutritious livestock feed. The availability of BSF populations strongly depends on mating success and reproduction. The mechanism of sex-ual selection during the mating period also determines the success rate of mating and reproduction and the survivorship of the offspring. Here, we analyzed how the influence of different mating status (virgin-ity) of mating pairs on mating success, daily oviposition, the number of eggs and fertility of eggs. BSF reared in semi-outdoor screen cages with five replication and four treatment of mates virginity combina-tion. An analysis of variance (ANOVA) was used to assess differences in mating and reproductive performance among treatment. Male and female BSF performed the remating activity. The virginity of males and females significantly influenced mating and oviposition frequen-cy. Mate choice was influenced by the virginity of mates. However, vir-ginity status of mates did not affect the number of eggs, eggs weight, and eggs fertility. Understanding of mate selection behavior in rela-tion to virginity in BSF served as important information to obtain the sustain population in the various scale of rearing design applicatÂ
Study on Sex Determination and Impact of Sex Ratio to Reproduction Success in Black Soldier Fly
Black soldier fly (BSF) (Hermetia illucens) is an insect spe-cies utilized as the converter of organic wastes into biomass applicable as a potential replacement of protein and lipid sources of animal feed and other industrial use. The limiting factor for the sus-tainability of this activity is the number of eggs produced as all processes started from the egg. Variable of quality of the substrate eaten during the larval stage and the proportion of males and fe-males are among the decisive factors for egg production. The pur-poses of this study were to understand the impact of the substrate to the production of a particular sex, to developed a simple method by comparing the weight and size of the pupae to distinguished between male and female, and to find the best sex ratio to produce the highest number of eggs. In this study, two types of substrates (balance proportion of macronutrient and high in protein and lipid were applied as feeding material for larvae. The weight and length of each pupa produced were measured and the pupae kept inside separated containers to find the morphological differences between male and female pupae. Adult insects produced from pupae then used for study on the effect of sex ratio on reproduction success. The adults kept inside 1 x 1 x 1 m screen cages with 3 groups of sex ratio (male : female) which were 50%:50% (P1), 60%:40% (P2), and 40%:60% (P3). The probability of sex was analyzed by logistic regression while the number of eggs estimated by the weight of the egg batch. The result showed substrate used in this study is not affect the sex ratio of adult flies, weight of pupae as the best predictor for sex of the adult flies, and the best ratio of male and female for egg production was 40:60.Â
Morphology Characterization of Rhopalocera in Two Areas of Resort Bukit Tiga Puluh National Park Riau Province
Bukit Tiga Puluh National park (TNBT) of Riau Province is a nature conservation area with a high diversity of fauna, one of which is the butterfly. Butterfly species diversity is influenced by the vegetation in their habitat. The TNBT utilization zone has a habitat type with varied vegetation. There is no information about the species of butterflies in the area. This study aimed to identify morphological character of butterflies and vegetation based on morphological characters. The method used was exploration. The catching area of the butterfly was the Granite waterfall and the Bukit Lancang path of TNBT utilization zone. Samples made as insectarium and herbarium. The  identification of butterfly characters consisted of the head, thorax, abdomen, legs and wings. There were a number of different types of butterflies and plants between the Granite waterfall area (22 and 28 species) and the Bukit Lancang paths (5 and 9 species). The type of butterfly was dominated by the family Nymphalidae. This is due to the amount of vegetation and environmental conditions that are more favorable in the granite waterfall area. The key determinants of butterfly species are the characters of the wing. The results of this study can contribute to maintaining the TNBT butterflies in Riau Province and also as database information of butterflies species in Indonesia
Studies on Fern of Lesser Sunda Islands I: Checklist of the Genus Adiantum (Pteridaceae)
Adiantum or maidenhair fern is one of the largest genera in Pteridaceae. We survey their species diversity in Lesser Sunda Islands, Indonesia through the living collection and herbarium examination. Seventeen species of Adiantum were recorded. including five species that considered as introduced and naturalized species. One species that grows naturally, Adiantum silvaticum, were previously known as endemic to Australia. However, this study revealed the new distribution of this species on Sumba and Flores