Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
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Studi Agronomis Tanaman Padi (Oryza sativa L.) Hasil Ko-Kultivasi Beberapa Strain Agrobacterium tumefaciens
Get PDFThe transgenic rice of Cisadane and Rajalele cultivars (cv) was obtained by co-cultivation of rice callus by 4 strain Agrobacterium tumefaciens containing 4 different binary vector (pIG 121 Hm, pCAMBIA 1301, pTOK 233 and pMOG22 BarGus). The transgenic plantlets were acclimatized and grown in the growth chamber to investigate their agronomic characteristic in comparison to control plants. The results showed that there was no difference in the number of spikelet and seeds/spikelet among different transgenic lines co-cultivated by different strains. The number of seed/spikelet of transgenic rice cv. Rojolele and Cisadane compared to their control did not show significant difference. However, the total number of spikelet was different only for cv. Rojolele
Jenis Kelelawar Pemakan Buah (Pteropodidae) di Taman Wisata Alam Gunung Meja Manokwari
Get PDFA study of bats was aimed to collect information on the presence of fruit bats that predicted involved in pollination or seed dispersal of certain commercial fruit plants within and around the Taman Wisata Alam Gunung Meja, Manokwari. Forty five individual consists of 8 (eight) species: Dobsonia minor, Dobsonia moluccensis, Macroglossus minimus, Nyctimene aello, Nyctimene albiventer, Nyctimene draconilla, Rousettus amlexicaudatus and Syconicteris australis have been captured and identified during the survey. It was assumed based on observation of vegetation found around the study site that the presence of fruit bats species help to maintain and contribute to seed dispersal of the fruit plants. Further study is required to obtain accurate data through the stomach content collection of the observed bats. Land conversion and illegal hunting was frightened influence the bat habitat and population which finally impact to fruit production
Biosorpsi Kadmium pada Leacheate TPA Gunung Tugel Menggunakan Biomassa Sargassum cinereum
Get PDFLeachate is liquid originated from organic waste. The substance consists of dissolved and suspended materials as a result of decomposition process by microorganisms. Leachate is waste consist of organic and high heavy metal. To manage leachate, heavy metal should be reduced by biosorption. Biosorption is heavy metal adsorbtion by inactive or death organism. The aims of the research were to examine the contact time, Sargassum cinereum biomass, combination of contact time and S. cinereum biomass to adsorb cadmium from the leachate. The experiments were based on Completely Randomized Design (CRD) with Spilt Plot Design. The treatments included contact time of Sargassum cinereum with leachate as main plot and S. cinereum biomass as sub plot. Contact time of S. cinereum with leachate consisted of three levels i.e., 1, 2 and 3 hours while S. cinereum biomass consisted of four levels, i.e., 200, 300, 400 and 500 mg. Data obtained were analyzed by analysis of variance (ANOVA) and followed by High Significant Difference (HSD) test. The results showed that S. cinereum biomass can adsorp cadmium from leachate. The level of adsorption of heavy metal was different in each treatment depending on contact time and S. cinereum biomass. The contact time of 3 hours was the optimum length in adsorping cadmium (39.168%) and 400 mg S. cinereum biomass was optimum amount in adsorping cadmium (38.936%). Combination of contact time 3 hours and 400 mg S. cinereum biomass was optimum treatment in adsorping cadmium (42.289%) from the leachate.
Hubungan Genetika Pria Masyarakat Sembiran dan Tri Wangsa di Bali
Get PDFBalinese community has evolved since the prehistoric era. The present community is structured into Hinduism caste (Brahmana, Kesatria, Wesia and Sudra), but remnants of their ancient communities, collectively called Bali Aga, have not undergone this structure. The first three castes of modern Balinese that comprises Tri Wangsa gentry were derived from JavanesekingdomofMajapahit(1343 AD). One of Bali Aga communities is Sembiran of Northern Bali. Using chromosomal microsatellite DNA, allelic variability of Sembiran and Tri Wangsa communities was reported. Both of them had the same high frequency of alleles which suggested genetic intermixing or sharing of common ancestor. However, a few alleles were unique for each community. This uniqueness was related to patrilineal system of Balinese community and to high mutation rate of Y chromosomal microsatellite DNA
Pola Dominansi Capit pada Uca spp. (Dekapoda:Ocypodidae)
Get PDFUca spp. are unique crab species marked with asymmetry cheliped. Major and minor cheliped has different function. The size of Major cheliped are several times of the minor ones (cheliped dominance pattern). Each species has different pattern of cheliped dominance. This study was to know the pattern of cheliped dominance on three Uca species. Major and minor cheliped length from twenty male individuals of U. dussumieri, U. vocans and U. perplexa were examined. The pattern of Cheliped dominance was obtained by comparing the major cheliped length to minor cheliped length. The two-tailed single sample t test showed similar cheliped dominance pattern within Uca species (P>0.001). While Kruskal-Wallis test showed different cheliped dominance pattern between Uca species (P>0.001). The cheliped dominance pattern of U. dussumieri, U. vocans and U. perplexa are 5.09, 4.24 and 4.75
Peran Paklobutrazol dan Pupuk dalam Mengendalikan Tinggi Tanaman dan Kualitas Bunga Krisan Pot
Get PDFThe objective of this study was to determine the effect of paklopbutrazol concentration and fertilizer on plant height and flower quality of potted chrysanthemum. The experimental method was used with factorial design. The first factor was paklobutrazol with the concentration as follow: 0, 100, 200, and 300 ppm. The second factors were cow, chicken, and goat manure. Parameters observed were plant height, time of flowers emerging, flower diameter and freshness. The results showed that certain concentration of paklobutrazol might inhibited plant height, time of flowers emerging, and shortening of flowers freshness, while chicken manure fertilizer spurred flower diameter
Rizofiltrasi Logam Pb (Plumbum) pada beberapa Jenis Tumbuhan Air
Get PDFThe research about rhizofiltration of Pb in several species of aquatic plant had been conducted in a greenhouse of Biology Faculty, Jenderal Soedirman University. The aims of this research were to evaluate the aquatic plant species which has Pb accumulative characteristic and to determine Pb concentration tolerated by those plants. This research was used experimental method with split plot design. The main plot was kind of aquatic plant of Hydrilla verticillata (L.f), Pistia stratiotes L, and Salvinia molesta D.S.Mitchell. The Pb (NO3)2 concentrations of 0, 5, 10, 15 mg/l were as sub plot. Each treatment was replicated for 3 times. The parameter observed were biomass (raw weight and dry weight), the Pb absorbed whether in root and shoot, and chlorophyll content. Data collected were analyzed using F test continued by LSD. The result of this research showed that both Pistia and Salvinia planted in 10 mg/l media were able to accumulate high Pb concentration. However, Pistia were able to accumulate Pb concentration mostly in root and only part be translocated in shoot. Whereas Salvinia was able to translocate Pb concentration in shoot higher than those in of Pistia. The implications of these results for rhizofiltration are discussed
Isolasi dan Identifikasi Kapang Saprofitik pada Sampel Tanah di Sekitar Kawasan Gunung Gamalama, Ternate
Get PDFMicroorganisms, such as mould and bacteria, have important roles in organic matter decomposition; and this process is fundamental to maintain soil structure and fertility. Moulds have hypha with filament structures that can penetrate to the substrates. Their enzymatic capability is higher than that of bacteria, especially in decomposing organic compounds like lignin and cellulose. The objective of the study was to isolate and identify saprophytic mould inhabiting soil of Mount Gamalama, Ternate, North Moluccas. The mould isolation was based on sample dillution method with Rose Bengal Cloramphenicol Agar isolation media. The abundance of saprophytic mould counted by measuring the average Colony Forming Unit (CFU)/ml of all mould colonies which grown on isolated media by Total Plate Count (TPC) method. The diversity of isolated mould was identified based on phenotypic characters by observing both macroscopic and microscopic morphology. The result showed that the average of mould colonies were between 13,30 x104 and 78,15 x104 CFU/ml. Identification based on morphology from selected mould isolates showed that 25 fungal taxa and two groups of unidentified fungal isolates were identified
Pengaruh Kerapatan Populasi Tanaman Sela dan Mulsa Plastik terhadap Pertumbuhan dan Produksi Jagung
Get PDFResearch of plant population density and intercropping of pigeon pie (Cajanus cajan L.) Millsp. and plastic mulching on maize were carried out in field research of R and D Centre for Biology, Indonesian Institute of Sciences, Cibinong from October 2008 to Februari 2009. The aim was to examine the effects of plant population density and intercropping, as well as mulching to the growth and yield of maize. The resultas showed that population density, intercropping and mulching affected the maize vegetative growth. However, the maize yield was not influenced by population density; but was influenced by intercropping and mulching. Mulching increased maize production, but intercropping by using C. cajan decreased production
Penentuan Konduktivitas Listrik dan Frekuensi Karakteristik Sel Ragi dengan Memanfaatkan Proses Dielektroforesis
Get PDFDielectrophorestic (DEP), a phenomenon where small particles such as yeast cells are manipulated by non-uniform electric fields. The net dielectrophoretic force is proportional to the polarisability of the cell and cause this cell moving toward higher field. By measuring velocity of the cell on the various frequency of the electric field, it can determine the electrical conductivity and the characteristic frequency of the yeast cells. The results showed that the conductivity of the yeast cell was (10,0 ± 0,4)10-9 Sm-1 and the characteristic frequency was 60 kHz