Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
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Pengaruh Limbah Organik terhadap Status Tropik, Rasio N/P serta Kelimpahan Fitoplankton di Waduk Panglima Besar Soedirman Kabupaten Banjarnegara
Get PDFThe increased of organic substance pollutans in waters as one of stimulate factors for phytoplankton growth. The input of organic matter from antrophogenic activity tend to influence the trophic state of reservoir. The aims of this research are to examine the influence of organic waste to Panglima Soedirman reservoir concerning the trophic state, Redfield ratio N and P, also the relation with abundance of phytoplankton. This research used survey methods with purposive sampling technique on 7 obsevation stations with 3 repeating conducted on May-July 2013. The result of the research showed that the input of organic matter increased in BOD5, COD and ammonia in reservoir and trophic state. Redfield ratio was shown 19,13-65,82 which means P as a limiting factor. Relationship between N/P with the abundance of phytoplankton with degree was strong correlated with chlorophyta and chrysophyta
Penentuan Jenis Kelamin Benih Ikan Betutu (Oxyeleotris marmorata Blkr.) dengan Teknik Truss Morphometrics
Get PDFA study on The Gender Determination Of Betutu Germ (Oxyeleotris marmorata Blkr.) by Truss Morphometrics used survey method with Simple Random Sampling design, Betutu’s germ as sample were taken from Penjalin reservoir, Brebes. The 26 distance of 13 pile points were based or Truss Morphometrics it were recorded as variables. The result of this research indicated that the size of Betutu’s A2 (origin anterior tip of snout at upper jaw – most posterior aspect of neurocranium), A4 (origin posterior point maxillary – origin of pelvic fin), B5 (origin of pelvic fin – origin of dorsal fin), B6 (origin of pelvic fin – origin of anal fin), D1 (insertion of anal fin – origin of lower caudal fin) and D3 (insertion of dorsal II fin – origin of lower caudal fin) can differentiate the gender of Betutu’s germ
Molecular Analysis of Synedrela Nodiflora (L.) Gaertn. Resistance Against Fomesafen using RAPD Markers
Get PDFBoth morphological and protein studies reveal that differences are observed between resistant Synedrella nodiflora against fomesafen and its susceptible wildtype. These are, however, more or less influenced by environmental factors, so that molecular analysis employing DNA markers is necessarily required. The methods involved total genomic DNA extraction using modified CTAB protocol following Doyle & Doyle (1990), RAPD marker amplification and visualization of RAPD markers prior to data analysis. Ten RAPD markers were used, but only seven of them showed polymorphism. Calculation of genetic distance and variation was carried out employing PopGen software.Based on the RAPD markers used in this study, it can be concluded that genetic distance between susceptible and resistant S. nodiflora is higher than that within susceptible samples supporting our previous morphological and protein data, although genetic variation among susceptible individuals seems to be significantly high
Transport Nutrien penyebab Eutrofikasi dari Daerah Tangkapan Air Waduk Mrica Banjarnegara
Get PDFThe transport of nutrien causing eutrophication in water from catchment area especially fosforus (P) through runoff is determined by type of landuses, slop, soils type, and land cover (Jeje,2006) . The research was conducted using a survey method. The sampling technique was done by a purposive sampling on any flooding in the rivers that pass through areas with specific land use (settlement, forest, plantations, agricultural fields and rice field). The main parameters were river discharge and the content of nutrients causing eutrophication that was phosphorus (P) in rivers. The P loadingfrom cacthment area to Mrica reservoir was 11,526,706.14 kg/year and the load allocation (LA) was 3,833,587.70 kg/year. The reservoir operational of power generating dan flushing activity were able to manage the LA up to 1,147,329.09kg/year, so the TMDL of Mrica reservoir become 4,980,916.78kg/year. It was concluded that the highest transport of P was from paddy field and then followed by respectivelly agricultural (potato) field, plantation (perhutani) field, settlement area, and forest area
Kajian Kualitas Air Sungai Condong yang terkena Buangan Limbah Cair Industri Batik Trusmi Cirebon
Get PDFThe aims of this study were to assess the water quality of the River Condong and Condong River Quality Statebased on physical, chemical and biological parameters. Sampling was carried out in seven stations with 3 repetitions at intervals of one month. Water quality were analyzed descriptively by reference raw Water Quality Standard. Determination of the stateof water quality using methods Storet. Results of laboratory tests showed that the water quality of the River Condong exceed the Water Quality Standard Group II based on Government Regulation No. 82 of 2001 and in particular textile waste quality standard TSS, DO, BOD5, COD, NH3, sulfide, Cr (VI), phenols, oils and fats, Biological parameters using makrobentos diversity index (H ') obtained a low value that is from 0 to 1.7077. Quality state based Storet method, the value of > -31. It can be concluded that the condition of physical, chemical and biological parameters, Condong River water showed waters not appropriate the Quality Standard for agricultural irrigation, freshwater fish breeding and infrastructure or water recreation facilities before and after the discharge of liquid waste of batik. Condong River quality state before and after the liquid waste of batik is heavily polluted
Keragaman Plankton dan Kondisi Perairan Tambak Intensif dan Tradisional di Probolinggo Jawa Timur
Get PDFThis study aims to estimate the wealth and stability of traditional and intensive brackishwater pond waters through biological index calculation (diversity, uniformity and dominance of plankton) in traditional and intensive brackishwater pond waters of Probolinggo Regency, East Java Province. Sampling plankton and water from the site, considered to represent the diversity of plankton and water stability of traditional and intensive brackishwater pond. Plankton was collected using a plankton net no. 25, then preserved using 1% lugol solution. Measurement of water quality variables include temperature, salinity, dissolved oxygen and pH, whereas the laboratory analyzes that Total Organic Matter (TOM), NO2, NO3, NH3, PO4, Total Suspended Solids (TSS), and Fe. Plankton identification using microscope and calculation with cell counting method. The results of analysis of plankton in intensive brackishwater pond waters are obtained as much as 23 genera consisting of 16 genera of phytoplankton were included into three classes, namely Bacillariophyceae as much as 5 genera, Cyanophyceae 3 genera, Dinophyceae and Chlorophyceae, each of the four genera, whereas zooplankton consisting of 7 genera are included into two classes, namely Crustaceae 6 genera and Rotatoria 1 genus. In traditional brackishwater pond earned as much as 13 genera consisting of 8 genera of phytoplankton were included into Bacillariophyceae 5 genera, Cyanophyceae 2 genera, and Dinophyceae 3 genera, whereas zooplankton consisting of three genera are included into class Crustaceae 2 genera and Rotatoria 1 genus. Plankton abundance in intensive brackishwater pond ranges 702 - 4269 ind./L, diversity index of 0.26 -2.38, uniformity index of 0.10 - 1.5, and dominance index of 0.02 - 0.68. In traditional brackishwater pond have an abundance of plankton range 134 - 776 ind./L, diversity index of 0.10 - 1.97, uniformity index of 0.08 - 1.0, and dominance index of 0.01 - 0.75. Traditional and intensive brackishwater pond waters condition still within the limits that can be tolerated by the culture organism. Based on the index value of biological, diversity of genus in the intensive brackishwater pond is relatively higher than traditional brackishwater pond and the community stability moderate, whereas traditional brackishwater pond unstable. On average, the uniformity of the genus in both brackishwater ponds are relatively evenly and genus of plankton to dominate the others genus
Variasi Morfologi Bambu Tali [Gigantochloa apus (Schult.F.) Kurz.] pada berbagai ketinggian tempat di Sub Daerah Aliran Sungai Pelus
Get PDFA study on the morphological variation of tali bamboo [Gigantochloa apus (Schult.f.) Kurz] has been conducted at various altitudes of Sub Watershed Pelus Banyumas, Central Java. This study was aimed at determining the morphological variations in relation to altitudes above sea levels. The method used was survey with stratified sampling. The variables measured included culm diameter, culm internode length, culm sheath length and width, ovate-triangular blade length and width, leaf blade length and width, stem color and hairs, and altitude. The results of this study indicated that the altitudinal difference effect some morphological characteristics of tali bamboos such as: 1) green young seedling with black hairs found at 200-299 m and 300-399 m asl. 2) morphological variations are effected by altitude. Tali bamboo grows optimally at the altitude of 400 to 499 m asl. The results of regression analysis showed that the altitude has a relatively close correlation with somes of morphological character of tali bamboo
Karakteristik Makro Algae Berzat Kapur di Perairan Tanjung Sira Lombok-Barat
Get PDFCoastal waters of Tanjung Sira has calcareousalgae of the genus Halimeda limestone, Padina, Amphiroa, Galaxaura, Corallina, Hydrolithon, Mesophyllum, Peysonallia, Porolithon and Sporolithon. The substrate that used as habitat are sand, coarse sand, rocks and dead coral rubble. Calcium carbonate contained on calcareous algae fungsioning as adhesive and encrusting dead coral, shells of mollusks that have decayed and massive objects in the waters of the sea. The research aims was to determine the growth characteristics of calcareousalgae in the reef flats, local distribution, calcium carbonate contain and its contribution as a frame work coastal reef waters. The research method using transect (Buckland et al., 1993). Identification of the type of aragonite and calcite according to Cordero (1977). Analysis of calcium carbonate according to Hillis (1980). The results showed that the green and brown calcareousalgae found in the reef flats, has thallus and tubers. Red calcareousalgae grew as encrusting on dead reefs and massif substrate. There are 16 species of calcareous algae that found in reseach area, 10 species containing aragonite mineral and 6 species containing calcite mineral. The content of calcium carbonate on each species obtained 100-450 g/m² consists of aragonite and calcite minerals. Calcareousalgae contribute in the new formation of coral reef ecosystems. The other benefit of calcareaousalgae in the coastal waters is an additional food for herbivorous fish. The content of calcium carbonate on calcareousalgae species is used in pharmaceutical field as drug ingredients and supplements for humans
Kajian Perubahan Bioekologi pada Restorasi Ekosistem Mangrove di Segara Anakan Cilacap
Get PDFMangroves are coastal ecosystems that have a very large role for humans and ecosystems in the vicinity. Mangrove condition in Indonesia, including in Segara Chicks Cilacap experiencing enormous pressure resulting in damage to the mangrove ecosystem. Mangrove restoration is the process of return of mangrove ecosystems of the conditions are broken into previously conditioned as well. The general objective of this study was 1) determine the conditions and amendments BioEkologi mangrove ecosystem restoration in the area of results Segara Chicks. Specific objectives in the study the first year is to determine: 1) the community structure of mangrove ecosystems (vegetation and fauna associations) at a restoration site in the region Segara Chicks, 2) the spatial variation community mangrove ecosystem in the area of restoration, and 3) the condition of the environmental factors that support the mangrove restoration in the region Segara Chicks. The method used was survey method with the technique of sampling cluster random sampling. The data obtained were analyzed multivatiate covering biodiversity analysis, cluster analysis, multi-dimensional sclae (MDS), and Bio-env using PRIMER-E program. The study shows the restoration of mangrove vegetation in the region have relatively low diversity (H ' 95%
Determination of Iron and Zinc Absorption by Local Isolates of Saccharomyces cerevisiae to Produce Iron and Zinc in Organic Form
Get PDFIron (Fe) and Zinc (Zn) play important role in health both of live stock and human. Fe and Zn in organic form were claimed increasing their viabilities. They bind to certain amino acid formed as a product of microbial metabolism. The Amount Fe and Zn absorbed may indicated the Fe and Zn organic produced. The aim of the study is to determine the absorption of microelement of Fe and Zn by local isolates of Saccharomyces cerevisiae to produce Fe and Zn in organic forms. S. cerevisae BCC F0205, BCC F0206, and BCC F0214 were treated with Fe or Zn 10 ppm to obtain S. cerevisae which has the highest of total concentration of Fe and Zn. Selected isolate was then treated with Fe or Zn respectively 2.5, 5, 10 ppm and their combination. Fe and Zn absorbed by isolates were measured by Atomic Absorption Spectrophotometer (AAS). The results show that S. cerevisae BCC F0205, BCC F0206, and BCC F0214 treated with 10 ppm Fe or Zn contained total concentration of Fe respectively 1.57, 3.07, 2.24 ppm and total concentration of Zn respectively 2.34, 3.20, 3.13 ppm. Then, S. cerevisae BCC F0206 treated with 2.5, 5, 10 ppm Fe or Zn, absorbed Fe 1.45, 0.50, 0.94 ppm and Zn 0.73, 0.38, 0.53 ppm respectively. Furthermore, combination of Fe and Zn (2.5:2.5, 5:5, 10:10 ppm) produce absorption of Fe 3.10, 2.13, 3.67 ppm and Zn 0.11, 0.10, 0.28 ppm per gram S. cerevisae BCC F0206. Percentages of absorption by S. cerevisae are up to 100% for Fe and up to 47.20% for Zn. In conclusion, this study indicated that S. cerevisae BCC F0206 absorb Fe and Zn higher than BCC F0205 and BCC F0214 and there are antagonistic interactions found between Fe and Zn in this research