Jurnal Sumberdaya Hayati
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Pelapisan Benih dengan Aktinobakteri untuk Meningkatkan Pertumbuhan Tanaman Padi: Actinobacterial Seed Coating for Promoting Rice Plant Growth
Get PDFThe need for rice continues to increase along with the increase in population. Efforts to increase rice production is generally carried out through proper and balanced fertilization. Other than that, plant growth-promoting actinobacterial (PGPB) inoculants can be used as an alternative solution. This study aimed to examine the effect of the application of actinobacterial seed dressing and NPK fertilizers on the growth of rice plants grown in a glass house. A randomized block design with three factors was conducted, consisting of actinobacterial seed dressing (added and not added), type of carrier (zeolite, peat, and combinations) and doses of NPK fertilizer (0 g/pot, 0.375 g/pot, and 0.75 g/pot). The addition of actinobacteria consortium, peat-zeolite combination 1:3, and NPK fertilizer at a dose of 0.75 g/pot (A2C3P3) gave consistent results in increasing the average yield of rice vegetative and reproductive parameters observed in the glass house. The A2C3P3 treatment had a significant effect on the number of tillers, width of flag leaf, dry weight of roots and shoots of rice observed at 10 WAP compared to other treatment combinations. The actinobacterial seed coating plays a pivotal role in supporting rice plant growth
Keanekaragaman Burung di Kawasan Babakan Siliwangi City Forest Path, Kota Bandung: Bird Diversity in Babakan Siliwangi City Forest Path, Bandung City
Get PDFIndonesia is a habitat for around 17% of the world’s bird species. Birds have an essential role, such as helping in pollination and seed dispersal, controlling insects, and as indicators of environmental change, hence their existence needs to be maintained to maintain the balance of the ecosystem. Babakan Siliwangi City Forest Path Bandung is an urban and green open area used as a public area allowing it to be a habitat for various birds. Therefore, this study aimed to determine the diversity and birds at that location. The method used in this study is the point count method with ten observation points and five repetitions with one observation line. The results showed five species of birds with 45 individuals from four families, namely Passeridae, Apodidae, Pycnonotidae, and Dicruridae. The index value of bird species diversity in this area is moderate (1.20). The Cucak Kutilang (Pycnonotus aurigaster) had the highest abundance of bird species (58%), while the lowest was the Srigunting (Dicrurus macrocercus) (2%). In addition, the evenness of bird species is high (0.74) with no dominance (0.39) and low species richness (1.05). Thus, the Babakan Siliwangi City Forest Path Bandung area has ecosystem conditions to support a bird’s habitat
Potensi Daun dari Enam Jenis Tumbuhan sebagai Pewarna Alami untuk Tekstil: The Leaf Potential of Six Plant Species as Natural Dyes for Textile
Get PDFPenggunaan pewarna alami untuk tekstil memiliki keunggulan, antara lain ramah lingkungan. Penelitian ini bertujuan menganalisis potensi pemanfaatan enam jenis tumbuhan sebagai pewarna alami tekstil. Penelitian menggunakan bahan daun dari pucuk merah (Syzygium oleana), kayu manis (Cinnamomum burnamii), alpukat (Persea americana), jakaranda (Jacaranda mimosifolia), kayu putih (Eucalyptus sp.) dan lanang (Oroxylum indicum); tiga jenis mordan yaitu tawas, tunjung, kapur sirih; dan tiga jenis kain yaitu katun combed, katun primisima, dan sutera. Tahapan penelitian meliputi ekstraksi zat warna dari tumbuhan dan pencelupan kain yang sebelumnya telah diberi perlakuan mordan. Potensi sebagai pewarna dianalisis berdasarkan warna yang dihasilkan dan stabilitas warna. Identifikasi warna dilakukan menggunakan RGB (Red Green Blue) Color Chart Reader. Stabilitas warna diukur dengan uji tahan luntur warna terhadap proses pencucian mengacu SNI ISO 105-C06-2010. Penerapan ekstrak pewarna pada jenis kain berbeda yang diberi perlakuan mordan berbeda-beda menghasilkan variasi warna. Selain itu, pemberian mordan juga berpengaruh baik terhadap ketahanan luntur warna akibat pencucian. Semua ekstrak menghasilkan warna dan ketahanan luntur terbaik pada kain sutera yang diberi perlakuan mordan tunjung. Semua jenis tumbuhan dalam penelitian ini berpotensi sebagai pewarna tekstil, namun untuk mendapatkan hasil terbaik dalam penggunaannya perlu mempertimbangkan jenis kain dan mordan.The use of natural dyes for textile have advantages since they are enviromentally friendly. This study aims to analyzed the potential use of six plant species as natural dyes for textiles. This research used leaves of pucuk merah (Syzygium oleana), cinnamon (Cinnamomum burnamii), avocado (Persea americana), jakaranda (Jacaranda mimosifolia), eucalyptus (Eucalyptus sp.), and lanang (Oroxylum indicum); three types of mordant (alum, ferrous sulphate, lime); and three types of fabrics (silk, combed cotton, primisima cotton). The research stages included extracting dyes from the leaves and dyeing fabrics that had previously been treated with mordant. The potential dye was analyzed based on the color and its stability on fabrics. The colors were identified using RGB (Red Green Blue) Color Chart Reader. Color stability was measured based on the fastness test against washing process referring to SNI ISO 105-C06-2010. The application of dyes on different types of fabrics which was treated with different mordants yield color variations. In addition, mordant has a good effect on color fastness. All extract showed the best color and fastness on silk fabric treated with ferous sulphate. The plant observed in this study have potential use as textile dyes, but it is necessary to consider the type of fabric and mordant to make the best results
Deteksi Bakteri Gram-Negatif Pada Permukaan Daun Kemangi (Ocimum basilicum) dari Tiga Pasar Tradisional di Bogor: Detection of Gram-Negative Bacteria on the Leaf Surface of Basil (Ocimum basilicum) Collected from Three Traditional Markets in Bogor
Get PDFOcimum basilicum or basil is commonly consumed raw by Indonesian. However, it is easily contaminated by pathogenic bacteria. This study aimed to detect Gram-negative bacteria in basil leaves obtained from three traditional markets in Bogor. Isolation and quantification of bacteria using serial dilution showed that the average number of bacteria on the surface of basil leaves was 9.6 × 107 CFU/g. Basil leaves obtained from the second traditional market had the highest number of bacteria of 11.3 × 107 CFU/g. All isolated bacteria have the same morphology namely bacilli, then further purified and characterized physiologically. The nine bacterial isolates obtained were able to grow on the selective-differential media including Salmonella-Shigella agar (SSA), bismuth sulphite agar (BSA), and eosin-methylen blue (EMBA). Gram staining showed that all bacterial isolates were classified as Gram-negative bacteria. The hemolytic ability of bacterial isolates was tested using blood agar base media, three out of nine bacterial isolates were able to produce hemolysin. Molecular identification based on 16S rRNA sequences showed that the bacterial isolates belonged to the Providencia sp., Proteus sp., Pseudomonas sp., and Kluyvera sp
Variasi Sisik Tumbuhan Paku Terestrial di Kampus IPB, Dramaga, Bogor : Variations of Terrestrial Scale Fern on The Campus of IPB University, Dramaga, Bogor
Get PDFIPB Dramaga campus has high biodiversity, one of which is terrestrial ferns. Ferns have scales or hairs that cover the rhizome, petiole and leaf blade. The characteristic of scales can be used in the identification of fern species. The aim of this study was to determine the morphological diversity of terrestrial fern scales collected from the IPB Dramaga campus. The fern scales was observed under a light microscope connected to an indomicro and the density was calculated. The rhizome and petiole scales found in the form of lanceolate, ovate, subulate, linear, and reniform. The tip of the scales varies from thick, and needle-like, thin, and thick. The edges of the scales vary, namely threaded, flat, or banded. The attachments of the base of the scales found were, peltate. pseudopeltate, and basifix. The color of the scales varies from transparent, yellow, light brown, fawn, and dark brown. The scale density ranged from 0.6 to 21.3/mm2. The Pteridaceae have more characteristics in common between their genera than the other two family, but these similarities cannot be used for taxonomic identification and classification, because they are not specific for each genus
Stok Karbon Organik Sedimen di Kawasan Ekosistem Mangrove Pesisir Kabupaten Kolaka Sulawesi Tenggara: Organic Carbon Stock in the Mangrove Ecosystem Area of Kolaka Coastal Line Southeast Sulawesi
Get PDFMangrove ecosystem can absorb and store carbon stock in their biomass and sediment. This ecosystem plays an important role in the carbon cycle. This study aimed to analyze the C-organic content and organic carbon stock of sediment, also CO2 absorption. The sediment sampling was conducted in the Kolaka Coastal line mangroves using a Peat Auger for 1 m of sediment depth. Then the sediment was separated into four layers. Then the sediment samples were oven dried at 50°C for 1 week for sediment bulk density analysis (SBD). The analysis of the C-organic content of sediment was done by the LOI method. The estimation of sediment carbon stock using sediment C-organic data and SBD data. The results showed that the highest average of C-organic content was in station 3 (4.12±0.15%). In the othher hand, the highest average of SBD was in station 1 (0.67±0.02 g cm-3). Overall, the total of sediment C-organic stock in station 1 was higher than other station (233.67±11.90 Mg C ha-1). The total capacity of CO2 absorption by sediment in the station 1 was also higher than other station (856.77±37.66 Mg CO2 ha-1). The sediment C-organic stock was influenced by C-organic content and SBD value
Peranan Pseudomonas aeruginosa ATCC 15442 terhadap Pertumbuhan Tanaman Cabai Rawit (Capsicum frutescens L.) Varietas Bonita pada Kondisi Salin: The Role of Pseudomonas aeruginosa ATCC 15442 on the Growth of Bonita Hot Pepper (Capsicum frutescens L.) Variety in Saline Conditions
Get PDFHot pepper (Capsicum frutescens L.) is one of the food crop commodities that is widely cultivated in Indonesia. Salinity stress can reduce the bioavailability of potassium and its uptake by plants, which will ultimately reduce plant growth and production. One way to reduce the effect of salinity and increase potassium uptake by plants is to use potassium-solubilizing bacteria. One of the bacteria that can solubilize potassium is Pseudomonas aeruginosa. This research aims to study the role of Pseudomonas aeruginosa ATCC 15442 on the growth of the Bonita variety of hot pepper under saline conditions. This study used a completely randomized factorial design with four concentration levels of NaCl treatment: 0, 4, 8, and 12 g/L, as well as two levels of bacteria administration: without bacteria and with bacteria. The results showed that the application of bacteria, salt concentration, and the interaction of the two had no effect (p-value> 0.05) on the growth of hot pepper plants with the observed parameters namely plant height, number of leaves, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, and chlorophyll content
Kepadatan dan Persebaran Parmarion pupillaris pada 10 Jenis Sayuran di Desa Dlangu, Kecamatan Trawas, Kabupaten Mojokerto: Density and Distribution of Parmarion pupillaris on 10 Species of Vegetables in Dlangu Village, Trawas District, Mojokerto Regency
Get PDFVegetables are one of horticultural commodities having a relatively short life cycle. Pest is one of the problems faced in vegetable cultivations. One specie that often attack vegetables is Parmarion pupillaris (Mollusca). It is a semi-slug that has a small bump transparent coat and shell on its back. These semi-slugs are often found eating leaves, stems, flowers and fruit of the vegetables. The aims of the study were to study distribution patterns, density, feeding activity and size distribution of the P. pupillaris in 10 species of vegetables in Dlangu Village, Trawas District, Mojokerto Regency. The observation plots were chosen randomly by determining three plots of each species sized 1m x 1m plot with three replications. Direct observation of feeding activities was conducted at night. Measurement of body length was conducted directly at the time of observation. Data analysis of density and distribution patterns used the quadratic method and Morisita Index. The results of this study showed that the highest P. pupillaris density found in tomato (Solanum lycopersicum), 8.7 ind / m2, while the lowest density was found in corn (Zea mays), 0.5 ind / m2. P. pupillaris found had medium and small sizes. Distribution patterns of semi-slugs based on the Morisita Index is categorized as clump
Isolasi Cendawan Mikoriza Arbuskula dari Rizosfer Tanaman Berkayu Asal Pulau Bangka dan Karakteristik Struktur Kultur Mikorizanya: Isolation of Arbuscular Mycorrhizal Fungi from Rhizosphere of Bangka Island Woody Plants and their Mycorrhizal Structure Culture Characteristics
Get PDFArbuscular Mycorrhizal Fungi (AMF) associate with a variety of plants including forest trees. Research on AMF in forestry in Indonesia is limited, especially on woody plants grown in Bangka Island. Therefore, the aim of this research was to isolate and identify AMF associated with woody plants from Bangka Island grown in the post-mining soil in greenhouse for 7 years. The Soil samples derived from 8 pots of the 7 years old woody plants were used as spore sources. Spore extraction from the soil used the wet sieving and decanting method. Fungal identification was carried out based on morphological characteristics, and fungal isolation used pot culture with Pueraria javanica as a host plant. Fungal structures within the root were analyzed after root staining with trypan blue. There were 18 spore morphotypes observed which belong to 5 types of Acaulospora and 13 types of Glomus. Seven single spore pot cultures were successfully isolated from species of Acaulospora sp.1, Acaulospora sp.4, Acaulospora sp.5, Glomus sp.2, Glomus sp.3, Glomus sp.6, and Glomus sp.11. AM fungal colonization structures observed within the root were Paris type arbuscules, internal hyphae, and vesicles. The AMF cultures obtained could be used as biofertilizer for woody plant seedlings production for post mining reclamation activities
Fenologi Fase Pembungaan dan Perbuahan serta Produksi Polen pada Tanaman Kacang Panjang Kultivar Sabrina: Phenology of Flowering and Fruiting Phases, and Pollen Production in Yardlong Bean Cultivar Sabrina
Get PDFYardlong beans (Vigna unguiculata subsp. sesquipedalis (L.) Verdc.) is a vegetable source of vitamins and minerals which are quite widely cultivated in Indonesia. Phenology study the phases that occur in a plant that is provides benefits in agriculture, but information on the phenology of legumes crops in Indonesia is still scarce. This study aims to observe the reproductive phenology and pollen production of the yardlong bean cultivar Sabrina. A phenological study was carried on 7 plants to obtain information on the timing and duration of the reproductive period, inflorescence and fruiting phases, peak flowering time, and flower biology. Pollen production was observed in 5 samples of flowers. Pollen microscopic preparations were made using the acetolysis method. The results showed that the flowering and fruiting phases took 21-29 days; including flower initiation phase 7-10 days, small bud phase 1day, large bud phase 1 day, anthesis phase 1-2 days, and fruit development phase 11-15 days. Flower initiation occurred 36 days after planting (DAP), and flower blooming occurred 49 DAP. The peak of flowering occurred at 56-62 DAP. The flowers of the Sabrina cultivar have purplish-white corollas, producing about 276±23.58 pollen/anther