Jurnal Sumberdaya Hayati
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Stek Pucuk Kentang (Solanum tuberosum L.) Kultivar IPB CP1 Dapat Menghasilkan Umbi G0 di Dalam Rumah Kaca dan Rumah Kasa di Dataran Menengah: Shoot Cuttings of Potato Plant (Solanum tuberosum L.) Cultivar IPB CP1 Can Produce G0 Tubers in Greenhouses and Screen Houses in the Med-Altitude Area
Get PDFIPB CP1 potato is a raw material for potato chips. The advantage of IPB CP1 potatoes is their high yield. Challenges in potato production are limited areas with optimal altitude and condition as well as low quality and quantity of seeds. Utilizing a mid-altitude area for potato cultivation is one of the solutions to increase potato seed production. This study aimed to compare the growth and production of IPB CP1 G0 seed tuber in a greenhouse and screenhouse in mid-altitude with different numbers of shoot cuttings per polybag. This research was conducted with two factors. The first factor is the use of growth plant houses, i.e. greenhouse and screenhouse. The second factor is the number of shoot cuttings per polybag, i.e. 2 and 3 shoot cuttings per polybag. Growth parameters observed included plant height, number of nodes and leaves, and fresh and dry weight of shoots. The production parameters observed included the number as well as fresh and dry weight of tubers. The growth and production of IPB CP1 potatoes grown in the greenhouse were better than those grown in the screenhouse. The number of cuttings per polybag did not affect the growth and production of IPB CP1 potato
Oral Klebsiella sp. Involved in Dental Caries: A Case of Individuals Gargling with Peatwater
Get PDFMost of the microbes inhabiting the oral cavities are harmless but can cause disturbance and discomfort if the microbial population increases significantly. One of the oral disorders that is often experienced by children, is dental caries. Dental caries is causedby various factors, including eating and brushing habits. The people of Central Kalimantan who live in the watershed use peat water in their daily activities, including for gargling and brushing their teeth. The acidic characteristics of peat water and the high content of organic substances trigger the development of microbes that cause dental caries.This study used dental caries swab samples of patients who rinsed their mouths with peat water. Bacteria were identified molecularly with 16SrRNA markers with primers 63F and 1387R. The similarity of nucleotide sequences was analyzed with BLAST on GeneBank. A phylogenetic tree was built with Maximum Likelihood. Both isolates have a fairly high level of similarity to Klebsiella pneumoniae, namely 91% and 93%. Both isolates are in the same clade, Klebsiella spp., and are close to various commonly found bacteria in the oral cavity, such as Streptococcus mutans and Lactobacillus salivarius
Perbandingan Komposisi dan Struktur Komunitas Makroalga antara Pulau Semak Daun dan Pulau Pramuka di Taman Nasional Kepulauan Seribu, DKI Jakarta: Comparison of Macroalgae Community Composition and Structure between Semak Daun Island and Pramuka Island in Kepulauan Seribu National Park, DKI Jakarta
Get PDFKepulauan Seribu have diverse biodiversity, one of which is macroalgae located in the Tourism Utilization Zone and the residential zone. Environmental factors, such as substrate, water quality and the presence of human activities, are very different on each island. The purpose of this study was to determine the differences in the composition and structure of macroalgae communities on islands in the Tourism Utilization Zone, Semak Daun Island and islands in the Settlement Zone, Pramuka Island. Settlement Zone, Pramuka Island. The method used is a quadrat transect. 16 types of macroalgae were found on Semak Daun Island and 26 types of macroalgae on Pramuka Island. The diversity value on Semak Daun Island is categorized as moderate with a value of 1.84 and on Pramuka Island is categorized as moderate with a value of 2.64. The highest INP value on Semak Daun Island is Sargassum binderi and the highest INP on Pramuka Island is Halimeda macroloba
Isolasi dan Karakterisasi Bakteri Rhizobium asal Bintil Akar Tanaman Kacang Tanah (Arachis hypogaea) dan Koro Rawe (Mucuna bracteata): Isolation and Characterization of Rhizobium Bacteria from Root Nodules of Peanut Plants (Arachis hypogaea) and Koro Rawe (Mucuna bracteata)
Get PDFNitrogen (N) is naturally available in the form of N2. Nitrogen fixation can be facilitated by microbes such as Rhizobium bacteria, which can establish symbiosis with the roots of legume plants to form root nodules that perform nitrogen fixation. This study aims to determine the characteristics of Rhizobium bacteria in the root nodules of peanuts (Arachis hypogaea) and koro rawe (Mucuna bracteata). The methods used in this research include collecting root nodules, isolating Rhizobium bacteria, and identifying and characterizing these bacteria for their nitrogen fixation and phosphate solubilization capabilities. Characterization results on YEMA + Congo red media indicated that 10 out of 16 isolates from peanut root nodules and 30 out of 55 isolates from koro rawe root nodules were positive for Rhizobium bacteria. The nitrogen fixation activity test revealed that only 4 isolates from peanut root nodules and 6 from koro rawe root nodules exhibited nitrogen fixation ability in Jensen\u27s medium. The highest phosphate solubilization index was obtained from isolate KK.5.1.1 (0.5±0.08), while the lowest was from isolate KK.5.2.2 (0.09±0.06). This research underscores the significance of Rhizobium in enhancing nitrogen availability and phosphate solubilization for legume plants
Inventarisasi Awal Liken Langka, Sensitif dan Toleran sebagai Bioindikator Polutan di Kabupaten Belitung Barat : Preliminary Inventory of Rare, Sensitive and Tolerant Lichens as Bioindicators of Pollutants in West Belitung Regency
Get PDFThe existence and population conditions of the rare, sensitive or tolerant lichens of Lobaria, Coccocarpia, Coenogonium, Dictyonema, Pseudocyphellaria, Sticta, Usnea, Leptogium, and Parmotrema tinctorum are not yet known in West Belitung Regency, so this research needs to be carried out. The method used in this research was exploratory descriptive with survey techniques and purposive sampling. The number of target lichen thallus was calculated from the entire area of the tree trunk with a height of 0-1.5 m. The lichens found in the Gunung Tajam area were Coccocarpia sp. with the highest number of thallus and the highest frequency of thallus (0.8%), followed by Coenogonium sp., Leptogium sp., and Usnea sp. with the same frequency of thallus (0.4%) while in the Batu Mentas area lichens were not found as the research target, but was dominated by fruticose lichen. Parmotrema tinctorum, the tolerant lichen, was not found at either location. The presence of sensitive lichens and the absence of tolerant lichens does not indicate that the locations have good air quality. It is recommended that the lichen population, especially P. tinctorum, be studied further so that air quality can be easily confirmed through the diversity and population of the lichens
Quantitative Analysis of Phytochemical Compounds and Antihyperglycemic Potential of Robusta Coffee from West Lampung
Get PDFWest Lampung Regency in Lampung Province produces high quality robusta coffee with a distinct, strong bitter flavour. Bitter flavour indicates the amount of bioactive substances produced by plants called phytochemicals. The aim of this study is to analyse the phytochemical compounds and antihyperglycemic potential of robusta coffee beans from West Lampung, Indonesia. Quantitative phytochemical analysis was carried out using the thin layer chromatography method and spectrophotometry. Animal experimental design using robusta coffee on hyperglycemia conditions in mice which was induced by alloxan 170 mg/kgBW subcutaneously and given by 1 ml of brewed robusta coffee for 10 days. The robusta coffee from West Lampung contained total caffeine 4,014.87 µg/g, total flavonoid content (TFC) 93.6 mg quercetin equivalent per gram of sample, with total alkaloid content (TAC) 0.848 mg of quinine equivalent per gram of sample, total tannin content (TTC) 182.3 mg of tannic acid equivalent per gram of sample, and total saponin content (TSC) 24.2 mg of quillaja bark equivalent per gram of sample. The administration of 1 ml/mice/day of robusta coffee brewed for 10 days did not show a decrease in blood sugar level in hyperglycemic mice, due to the short duration of the study, so the role of coffee in decreasing hyperglycemia conditions has not been optimally observed
Analisis In Vitro Aktivitas Antioksidan Ekstrak Aseton dari Ranting Horsfieldia macrothyrsa Menggunakan Beragam Metode: In Vitro Analysis of Antioxidant Activity of Horsfieldia macrothyrsa Twig Acetone Extract Using Various Methods
Get PDFHorsfieldia macrothyrsa (Miq) Warb. is a plant that contains secondary metabolite compounds that can act as antioxidants. This research aims to determine the antioxidant potential of the acetone extract of the H. macrothyrsa plant by looking at the IC50 value. Twigs of the H. macrothrysa plant were extracted using the maceration method using acetone solvent. The results of the antioxidant activity test using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method showed very strong antioxidant activity with an IC50 value of 6.348 µg/ml whereas the IC50 value of the standard antioxidant quercetin is and quercetin 1.261 µg/ml. In antioxidant testing using the ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) method, an IC50 value of 12,772 µg/ml was obtained and FRAP (Ferric Reducing Antioxidant Power) obtained an IC50 value of 7,511 µg/ml. Based on the results, it can be concluded that the acetone extract of H. macrothrysa plant twigs has very strong antioxidant activity towards reducing DPPH (2,2-diphenyl-1-picrylhydrazyl) compared to using the ABTS and FRAP methods
Karakterisasi dan Potensi Acremonium sp. Secara In Vitro Terhadap Cendawan Patogen Fusarium oxysporum: Characterization and Potential of Acremonium sp. Against Pathogenic Fungi Fusarium oxysporum In Vitro
Get PDFVarious natural interactions exist between fungi and plants, including mutualism and pathogenesis. This study evaluated the interaction of fungi Acremonium sp. to plants and pathogenic fungi. Acremonium sp. was isolated from the root tissue of an oil palm plant using surface sterilization methods before isolation. The potential pathogenicity interaction of Acremonium sp. was assayed towards Chinese white cabbage (Brassica rapa L. var. pekinensis) in vitro. Based on the PDA medium assay, isolated Acremonium sp. exhibited pathogenic traits against Chinese white cabbage within 14 days post-inoculation. Interestingly, Acremonium sp. showed antifungal activity against Fusarium oxysporum IPBCC.19.1468, with an average inhibition rate of 69.11%. Our data suggest the pathogenicity interaction of Acremonium sp. toward cabbage plant and fungi F. oxysporum IPBCC.19.1468
Perbandingan Ukuran dan Komposisi Tubuh antara Mahasiswa Laki-Laki Atlet dan Non Atlet Universitas Padjadjaran: Comparison of Body Size and Composition between Male Student-Athletes and Non-Athletes at Universitas Padjadjaran
Get PDFBody composition is the relative proportion of fat and fat-free tissue in the body and indicates health profile. Athletes usually have a different body composition compared to the general population due to their involvement in intense sports activities. This research investigated the body size and composition among male student-athletes and non-athletes at Universitas Padjadjaran. The study used a cross-sectional method and anthropometric measurements on 14 athletes and 34 non-athletes. Anthropometric data consisted of height, weight, body fat, visceral fat, skeletal muscle, and Body Mass Index (BMI). Student-athletes have a lower average weight, body fat, and BMI than non-athletes. They tend to have body fat and visceral fat in the normal category (50% and 100%), whereas non-athletes (5.9% and 79.4%). Additionally, student-athletes have more skeletal muscle (63.4%) than non-athletes (47.1%). Furthermore, nutritional status based on BMI values indicates that student-athletes tend to fall within the normal category at 78.8%, while non-athletes are only at 44%. These results demonstrate that the body size and composition of student-athletes reflect better physical fitness, resulting in a better nutritional status than non-athletes at Universitas Padjadjaran
Karakterisasi dan Potensi Antibakteri Ekstrak Kapang Endofit Hasil Isolasi dari Sargassum sp.: Characterization and Antibacterial Potential of Endophytic Fungi Extracts Isolated from Sargassum sp.
Get PDFThe bioactive potential in Sargassum sp. and its direct use is expected to reduce its population in nature if used continuously. Using endophytic fungi from the host is a solution for obtaining bioactive compounds from Sargassum sp. The purpose of this study was to characterize endophytic fungi in Sargassum sp. and its potential as an antibacterial agent. Endophytic fungi were isolated using the direct planting technique, endophytic fungi were characterized through microscopic and macroscopic observations. Extraction was performed with two organic solvents, namely ethanol and ethyl acetate. Antibacterial potency was tested using the agar diffusion method using Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Salmonella typhi. Based on the results, two mold isolates, D3P1 and D4P1, were obtained. The ethyl acetate extract of D3P1 had the highest inhibition zone for Bacillus subtilis bacteria, with the highest inhibition zone being 23.2 mm for Bacillus subtilis and the smallest being 17 mm for Escherichia coli. The ethyl acetate extract of D3P1 had the highest inhibition zone compared to the ethyl acetate extract of D4P1 and the ethanol extract of D3P1 and D4P1. The GC-MS analysis identified four compounds that have been identified in the ethyl acetate extract of D3P1 isolates, among others, 2,3-Butanediol, Phthalic acid, 1,2-Benzenedicarbocylic acid, and 1-Anthracenamine. Phthalic acid and 1,2-Benzenedicarboxylic acid compounds have the potential as antibacterial