Jurnal Sumberdaya Hayati
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Protease from Courgette (Luffa Acutangula L (Roxb)): Isolation, Purification, and Some Characteristics
Get PDFThe Courgette or oyong (Luffa acutangula L. (Roxb)) is member of Cucurbitaceae mainly used as vegetable. Beside used as vegetables, courgette aslo used as keratolytic agent. This fact is supposed that this vegetables contain protease. This research is succeed to purified courgette’s protease by four step. That was precipitate by 70% ammonium sulphate saturation, purification using DEAE cellulose ion exchange chromatography and gel filtration chromatography using sephadex G-100 and G-75. Purified courgette’s protease had 81,922 U/mg for specific activity and 34 kDa molecular weight. This enzyme had the characteristic such as activated optimally at 37oC, pH 7 and 10 minute duration time. This enzyme activity can decrease by PMSF and H2O2, its remarkable that courgette protease is serine protease and had the thiol group in its structure. The ability to digest food proteins materials like boiled meat and boiled white egg by courgette protease proves that the courgette protease enzyme is could be used in enzyme replacement therapy in mild digestion problem. 
Perilaku Harian Monyet Hitam Sulawesi (Macaca nigra) pada Masa Kebuntingan di Cagar Alam Tangkoko-Batuangus, Sulawesi Utara: Behavior of Crested Macaque ( Macaca nigra ) During Pregnancy at Tangkoko-Batuangus Nature Reserve, North Sulawesi
Get PDFCrested macaque (Macaca nigra), one of the seven endemic primates in Sulawesi is the most endangered macaque species that has been categorized as critically endangered by International Union for Conservation of Nature (IUCN). However, almost nothing is known about the reproductive biology in associated with the behavioral during pregnancy. The data is important to support a management and breeding programs such as pregnancy diagnostic and conservation program in this species. The research aimed was to study the behavior of the crested macaques during pregnancy. Observations were performed on the 10 adult females of the group of Rambo 1 (R1) which has been habituated at Tangkoko-Batuangus Nature Reserve, North Sulawesi. Behavioral data were collected using focal animal sampling and recorded using instantanaeous sampling on females during menstrual cycling, pregnancy, and after parturition (lactation). During five months observations, a total of 393.63 observation hours were recorded. The results showed that daily activities of the M. nigra during menstrual cycling were dominated by feeding (26.60%), foraging (15.95%), and locomotion (16.16%). Daily activities during pregnancy was dominated by feeding (27.03%), foraging (16.37%), and resting (13.45%), whereas after parturition (lactation) period is dominated by resting (26.12%), foraging (19.51%), and feeding (14.98%). The resting was the most activity in the end of pregnancy and few day after parturition. The diets were collected successfully composed of the fruits (34 species), arthropods (3 species, 2 families, 4 orders), fungi (4 genus), and plants (5 species). Climate was influenced on the composition of the diet, especially on fruit and arthropods. In conclusion, the activity budgets were different during menstrual cycling, pregnancy, and lactation, especially feeding, foraging, locomotion, and resting. 
Kopi Arabika-Gayo Menurunkan MDA dan Meningkatkan SOD setelah Latihan Fisik Akut Submaksimal pada Pria Sedenter: Gayo-Arabica Coffee Decreases MDA and Increases SOD after Single Bout Submaximal Physical Exercise in Sedentary Men
Get PDFIt is known that coffee has much phenolic components such as flavonoids and chlorogenic acids (CGAs) content for neutralizing oxidative stress. Gayo-arabica coffee is a typical arabica coffee which widely used by Gayo people in Aceh, Indonesia. The coffee plants also grow in Gayo. However, it has not clearly understood its effect for health. The study aimed is to analyze the Gayo-arabica coffee consumption on the levels of malondialdehyde (MDA) as an indicator of damage cells due to oxidative stress and superoxide dismutase (SOD) as a marker of antioxidant enzyme, after performing a single bout submaximal physical exercise. Male sedentary subjects (21-27 y.o), consisted of 36 men, divided into 3 groups: control (zero of coffee), Gayo1 (15 g coffee in 200 ml water), and Gayo2 (20 g coffee in 200 ml water). Coffee was given once, after rockport test as a single bout submaximal physical exercise. MDA serum and SOD serum were taken after 1 hour of coffee drinking. MDA serum decreased significantly in Gayo1 (8.01 uM) and Gayo2 (10.36 uM), compared to control (26.82 uM). SOD serum increased significantly in Gayo1 (114.81 ng/ ml) and Gayo2 (101.48 ng/ml), compared to control (24.024 ng/ml). Nevertheless, there was no significantly different between Gayo1 and Gayo2 on MDA and SOD serum (p≥0.05). We suggest that the Gayo-arabica coffee after single bout submaximal exercise can diminish stress oxidative in sedentary people. 
Biodiversitas Kumbang Koprofagus di Lahan Pertanian Organik dan Non-organik: Biodiversity of Coprophagous Beetles in Organic and Non- organic Farms
Get PDFCoprophagous beetle has important role in decomposition of organic materials. This research aimed to study and analyze the diversity and abundance of coprophagous beetles in organic and non-organic farms. Coprophagous beetles were collected in organic farm of Bina Sarana Bakti and non-organic farm of Seruni in Cisarua, West Java. In each location, twenty dung traps were set up in corn plants, carrot plants, cabbage plants, and onion plants. Totally, 15 species (610 individuals) of coprophagous beetles belonging to two families i.e Scarabaeidae and Aphodiidae were collected. Coprophagous beetles in organic farms (389 individuals, 13 species) more divers than that in non-organic farms (221 individuals, 10 species). There was no dominant species in both of locations (C<0.5). The most dominant species in organic farm was Copris reflexus (49%) and in non-organic farm was Onthopagus pauper (52%). Beetles similarity in organic and non-organic farm was 67%. 
Perkembangan Morfo-anatomi Bunga, Buah, dan Biji Nyamplung (Calophyllum inophyllum L), Sebagai Tanaman Penghasil Biodisel: Morpho-Anatomy Development of Flower, Fruit and Seed of Nyamplung ( Calophyllum inophyllum L) as Biodiesel-Producing Plant
Get PDFNyamplung (Calophyllum inophyllum), a non-edible producing plant, is a tropical plant traditionally used by local residents as firewood, building material and medicinal plants. The research aimed to study the development of flower and fruit as well as oil content of nyamplung seeds. Two adult trees (P1 and P2) with different provenance were used in experiment. Morphology of flower and fruit, and seed germination were observed from the field, while the microscopic anatomical observations and oil content analysis were carried out in the laboratory. The result showed that a single generative bud generally had 8-15 flowers. The flower was arranged in bunches, with 4 petals, 4 sepals, single pistil and 4 files of anthers surrounded the pistil. In some cases, the flowers had 5 sepals, or 3 sepals and 3 anthers files. Fruits were round, green and turned to harden with brownish color during maturation. Flower bud grown from bud was ready to blossom 27 days after initiation (DAI). There were up to 48.52% flower buds drop at 24 DAI, and they were still increase up to 64.5% flower drop during the whole of flower development. The observation also showed that fruit drop rate of P2 plant was higher than P1. After flower bloom for 3 or 4 days after anthesis, the flowers started to lose their parts. The fruits started to ripe 8-10 week after anthesis (WAA). Seeds from P2 tree had oil content of 51.79%, which was higher than the P1 tree (42.57%). 
Potensi Cendawan Asal Hydrilla verticillata Sebagai Pengendali Hayati Fusarium oxysporum dan Ganoderma boninense: Fungi from Hydrilla verticillata as Potential Biological Control Agents of Fusarium oxysporum and Ganoderma boninense
Get PDFHydrilla verticillata is an aquatic weed, can build symbiosis and also become a substrate of freshwater fungi. Some of freshwater fungi that live in association with H. verticillata are pathogenic fungi which were potentially used as controller for Fusarium oxysporum and Ganoderma boninense. The use of pathogenic fungi as a biocontrol is not consistent in the field. Therefore, this research aimed to find new fungus candidates that are stronger and more consistent in controlling pathogenic fungi. Freshwater fungi were isolated from H. verticillata. Identification was based on morphological characters. Molecular analysis was carried out for freshwater fungi which had the highest inhibition percentage to pathogenic fungi. The antagonism test was conducted by dual culture method. The mechanism of interaction of hyphae was observed under a microscope. Freshwater fungi was derived from H. verticillata stem are Aspergillus section flavi, Gliomastix sp., and Talaromyces sp. Antagonism test results showed that all fungi could inhibit the growth of pathogenic fungi. Based on molecular analysis used ITS region showed Talaromyces sp. could not identify to the level of species so that additional genes were required, namely beta-tubulin gene, in order to further identify Talaromyces sp. 
Ragam Jamur Asal Serasah dan Tanah di Taman Nasional Ujung Kulon Indonesia
Get PDFBiodiversity is an important aspect which supports life systems on earth as well as maintains ecosystem sustainability and evolution. Fungal communities, in particular, saprophytic fungi, are often ignored during an inventory of biodiversity in the National Park Area. Saprophytic fungi is a fungus obtain nutrients by decomposing dead organisms or other organic material and act as decomposers in the ecosystem. Saprophytic fungi can be found growing on dead organic matter contained in the environment such as soil, wood, and litter. The diversity of Macroscopic saprophytic fungi from Ujung Kulon National Park is reported here. Fungal identification was done by using a variety of macroscopic characters. This study determined eight species that classified into six genera of saprophytic fungi, namely: Collybia, Crepidotus, Hygrocybe, Lepiota, Marasmius, and Mycena. By knowing the diversity of saprophytic fungi, it is expected to add information about biodiversity as a preparation towards the further development of the potential of existing biodiversity in Indonesia.
Kompleksitas Obyek dan Running-Wheel Mempengaruhi Novel Object Recognition Test pada Mencit (Mus musculus)
Get PDFThis research aimed to confirm the tendency of mice to novel object, effect of exercise (in running-wheel) toward memory of mice and to test tendency of mice in avoiding predator signal in novel object. Novel object recognition test (NORT) used to test the memory the day after acquisition phase (NORT I) and memory one week after exercise was given (running-wheel) (NORT II). The result showed that there was no tendency of mice in exploring toward novel object in both NORT I and NORT II. This might happen because the complexity of familiar object higher than novel object, so the familiar object could accommodate more activities. Exercise using running-wheel in mice had an effect on memory, it could be seen in decreasing duration of object exploration time from NORT I to NORT II. There was no tendency in avoiding predator’s signal on novel object which was attached by urine addition (odor signal)
Kultivasi Bawang Merah Ramah Lingkungan di Wilayah Berpasir Spesifik di Yogyakarta
Get PDFShallot is one of strategic commodities in Indonesia. Sandy coastal area of Bantul and Kulon Progo districtis the centre of shallot production in Yogyakarta. The results showed that recommendation of fertilizing technology ameliorant (clay 2:1), organik fertilizer 20 ton/ha, Urea 115.7 kg/ha, ZA 250 kg/ha, SP-36 75 kg/ha, and KCl 250 kg/ha. Pest and disease management in integrated pest and disease management 10 Feromon – Exi, Attractant, 10 Light trap, 45 Yellow/white/green trap, biopesticide, limited chemical pesticide, organophosphate pesticide residue contents below with residues below the MRL
Karakterisasi Morfologi dan Anatomi Tanaman Manggis dan Kerabatnya (Garcinia spp.) di Taman Buah Mekarsari
Get PDFMangosteen (Garcinia mangostana) cultivation has problems in terms of fruit quality and rooting system. This problem is solved by Mekarsari Fruit Garden by developing the technology of double rootstock seedling that is by combining two mangosteen seedling into one plant through the pasting process. Characterization of the collection of mangosteen plants and its species of relatives (Garcinia spp.) is needed to seek superior characters used for the technology of double rootstock seedlings, cultivar development, and management and preservation of mangosteen germplasm. This study aims to record and compare morphological and anatomical characters of mangosteen and its relatives collected in Taman Buah Mekarsari. The study was carried out by collecting the sample and characterizing the morphological structure using mangosteen descriptor, observing the leaf paradermal anatomy, and collecting secondary data. Observation on the morphological characteristics of mangostana (G. mangostana) and its relatives (G. celebica, G. dulcis, G. forbesii, and G. malaccensis) showed variations on the fruit characters, such as the shape is spherical, ovate or spherical flattened apex and base; the rind color is bright yellow, deep red, and bright red; the flavor is sour, sour sweet, or sweet. The anatomical character of the mangosteen leaf and four species of its relatives show the paracytic stomata on the abaxial surface only except on the leaf of G. forbersii, which is found on the abaxial and adaxial sustance, the epidermal cells are square to rectangular, the wall is straight or slighty winding except that of G. forbesii which is sharply winding or zig-za