HAYATI Journal of Biosciences
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Genome-wide Screening of Zinc-Responsive Genes in Arabidopsis thaliana for Enhanced Phytoremediation Potential
Full text linkZinc contamination presents considerable threats to human health, agricultural practices, and ecological systems. Arabidopsis thaliana has demonstrated potential for use in phytoremediation, which is an environmentally friendly strategy aimed at addressing this type of pollution. This study identified genes associated with phytoremediation in A. thaliana. This identification was achieved through comparative bioinformatics analysis involving 27 plant species. A critical literature review was conducted. This review aimed to collect genes involved in metal uptake and transport across various hyperaccumulator plant species. A database in BLAST format was generated to enable the study of main homologous protein families, including ATP-binding Cassette transporters, acyl-CoA-binding proteins, metallothioneins, and phytochelatin synthases. In silico screening for zinc-binding was performed by means of a web application based on the deep-learning framework called GNINA. The results highlighted the fact that within the phytochelatin synthases-especially PCS1 and PCS2, the protein PCS2 has the highest affinity to zinc. Heavy metal ATPases involved in the vital process of metal transport and homeostasis include HMA1. Zinc transporters responsible for zinc uptake and sequestration include cation exchanger 1CAX1 and IRT2. The information thus provided explains in detail the complex molecular machinery regulating metal detoxification in A. thaliana and provides insights for developing transgenic plants with enhanced phytoremediation capabilities. Future research should target these genes to create plants with improved metal tolerance and accumulation, advancing phytoremediation technologies
The Antiaging Potential of Serum Formulations from Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA on Injured Human Fibroblast Cells
Full text linkExternal aging factors such as UV exposure, pollution, and lifestyle choices contribute to skin aging, resulting in deep wrinkles, fine lines, and rough skin, which can lower self-confidence. Plant extracts have been widely studied for their antiaging potential, while Salmon DNA has shown promise in stimulating collagen production. This study explores the formulation of a serum combining Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA for its antiaging effects on injured human fibroblast cells. The serum was formulated using extracts from C. asiatica, C. longa, A. vera, R. centifolia, and Salmon DNA. Antioxidant activity was evaluated with the DPPH method, cytotoxicity using the WST-8 assay, and gene expression through qRT-PCR for COL1A1, TGF-β1, HYAL-1, and FGF-2. The serum exhibited weak antioxidant activity (IC50 = 373.33 µg/ml) and reduced cell viability at high concentrations. Gene expression analysis revealed increased expression of COL1A1, TGF-β1, and FGF-2, along with reduced HYAL-1 expression in injured BJ cells. The formulated serum shows potential as an antiaging agent, promoting collagen production and reducing hyaluronidase activity
Influence of Co-feeding Methanol-sorbitol Ratio on Production of Human Insulin Precursor Expressed by Mut+ Pichia pastoris
Full text linkAn increasing number of diabetic patients and the demand for insulin encourage the development of recombinant insulin production on a large scale. Human insulin precursor (HIP) expressed by Mut+ Pichia pastoris using methanol as an inducer was developed. However, methanol above 5% (w/v) or 1.56 M is toxic for the host. Sorbitol was introduced as a co-substrate with methanol. To our knowledge, the study of methanol/sorbitol co-feeding on human insulin precursor (HIP) expression by Mut+ Pichia pastoris in a bioreactor has yet to be reported. This study aimed to investigate the influence of the methanol-sorbitol co-feeding ratio on the expression of HIP expressed by P. pastoris X33 Mut+. The study was conducted by comparing the cultivation of P. pastoris/pD902-IP Mut+ in a 10-liter bioreactor under three conditions: feeding 100% methanol, mass ratio of MeOH:sorbitol 12:1 and 3:1. The oxygen consumption of methanol/sorbitol is less than the methanol feeding. The mass ratio of MeOH:sorbitol 12:1 produced the highest HIP titer (1326.5 mg/L), 1.5 times higher than methanol feeding, the lowest specific growth rate, but the highest specific productivity at the induction phase. MeOH:sorbitol mass ratio 3:1 produced the highest dry cell weight (DCW) amount (96 g/L). These results suggested that an appropriate ratio of sorbitol-methanol can be a choice to replace methanol feeding in a Mut+ P. pastoris
The Potential Use of Wood Vinegar as an Alternative Inhibitor to Enhance 5-Aminolevulinic Acid Production by Bacillus paramycoides
Full text linkThe expensive production of 5-aminolevulinic acid (ALA) as a plant growth stimulator led to an attempt to get an alternative substrate to reduce the production cost. Levulinic acid is the inhibitor of ALA dehydratase in ALA production. Wood vinegar, a liquid gas produced from wood combustion in airtight conditions, is known to contain 12-17 mM of levulinic acid. This study aims to explore the use of wood vinegar as a potential substitute for levulinic acid in the extracellular production of ALA by bacteria identified as Bacillus paramycoides through 16S rRNA sequence analysis. Adding precursor and inhibitor glutamate and 1% wood vinegar increased the ALA production to 174.3 µM, while the combination of glutamate and levulinic acid raised the ALA production to 179.9 µM. This study confirmed that wood vinegar can enhance the concentration of ALA and potentially substitute levulinic acid as an inhibitor in ALA production
Phytochemical Analysis and Pharmacological Properties of Epipremnum pinnatum (L.) Fruit Extracts
Full text linkEpipremnum pinnatum (L.) Engl., belonging to the family Araceae, is a medicinal plant used by locals in many Asian countries to treat common ailments such as wounds and pain. In this study, the different parts of the fruits -peel, core, and kernels were analyzed for their phytochemicals and antioxidant potentials. Initial phytochemical investigation revealed that flavonoids, polyphenols, and sterols were found in all parts. Terpenoids were found in the core and peel extracts, while cardiac glycosides were found in the core and kernel extracts. The peel extracts were found to have the highest flavonoid content (308.9±19.2 mg quercetin equivalent per g fresh wt sample). It also exhibited the highest total antioxidant activity (EC50 = 23.1±4.2 ppm) among the three extracts, which is next to the activity of ascorbic acid (EC50 = 4.6±1.6 ppm) using the phosphomolybdate method. It also gave the highest DPPH-free radical scavenging activity (EC50 = 41.9±2.6 ppm) among the three extracts but lower activity than the ascorbic acid (EC50 = 1.1±0.5 ppm). At 200 ppm, the peel extracts exhibited a profound inhibitory effect (>50%) on the melanin production in zebrafish embryos. But, at this concentration, the peel extracts were also found to be toxic to the embryo, with 43% mortality. However, the extract did not exhibit antibacterial properties against E. coli and S. aureus. These results suggest the potential of the fruit parts, particularly the peel, as a source of natural compounds with pharmacological importance
Oxidation Stress of UV C Light on Growth, Carotenoids and Chlorophyll-a Content of Chlorella vulgaris
Full text linkC. vulgaris is photoautotrophic, which refers to microalgae that require light as an energy source. One of the light sources used to affect pigments in microalgae is UV light. The advantage of UV light is that it can change gene composition, which results in mutant genes in biopigment mutations and causes a higher number of activities compared to the natural ones, thereby increasing the growth, carotenoids, and chlorophyll-a of microalgae. The aim of this research is to determine the effect of oxidation stress of UV light on growth, carotenoids and chlorophyll-a content of Chlorella vulgaris. The treatments were given based on differences in the power of the UV-C light from, 8W, 15W and 30W. This study used the UV-Vis Spectrophotometer method was used to calculate growth, carotenoids and chlorophyll-a. Data were analysed using ANOVA test, Duncan\u27s Multiple Range Test, and Principal Component Analysis (PCA). The results showed a significant effect of UV-C light. The best treatment was found in the administration of 30 W UV light, with the results of growth was 4.7746 × 106 cells/ml, content of carotenoids was 1.75 mg/ml and chlorophyll-a was 4.67 mg/ml of C. vulgaris. Biopigment can absorb radiation from UV-C light, which causes these microalgae to survive in environmental stress conditions. Absorbed light can affect the spectrum of pigments, thereby increasing growth, carotenoids, and chlorophyll-a
Detection of the Yersinia enterocolitica Bacteria Targeting the myfA and ystA Genes in Contaminated Vegetable Samples using Real-Time PCR to Develop Rapid Detection of Food Poisoning Bacteria
Full text linkYersinia enterocolitica is a pathogenic bacterium with the ability to survive and multiply in food in a low-temperature environment that can cause death in humans. In previous studies, the optimum annealing temperature of ymoA, ystA, and ail gene primers with amplicons of 185 bp, 123 bp, and 192 bp, respectively, was successfully found. This study aims to develop a pathogenic bacteria detection kit with confirmation, sensitivity, and specificity of myfA and ystA primers in detecting Yersinia enterocolitica bacteria quickly and accurately using the real-time Polymerase Chain Reaction method. The results showed that myfA and ystA primers have optimum annealing temperatures at 60°C with amplicon lengths of 181 bp and 123 bp, respectively. Primer myfA was able to amplify the target with real-time PCR at Ct 12.07±1 and Tm 81±1°C, while the ystA primer at Ct 12.38±1 and Tm 83±1°C. myfA and ystA primers were also able to distinguish target and non-target bacteria based on Ct or Tm. The designed primers successfully detected Yersinia enterocolitica bacteria with the smallest concentration of 0.000439 ng/µL equivalent to 7.024 × 102 CFU. The detection limit obtained is smaller than the contamination threshold set by the Food and Drug Administration (BPOM). Primer myfA and ystA Yersinia enterocolitica also successfully detected the target bacteria in cabbage and lettuce samples artificially. Based on these results, myfA and ystA primers successfully detected Yersinia enterocolitica in vegetable samples using real-time PCR quickly, sensitively, specifically, and accurately
Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis
Full text linkGalangin has activity modulating cell cycle arrest on luminal cancer cells and has high selectivity and low cytotoxicity for normal cells. This research intends to know galangin\u27s prospective targets for promoting cell cycle arrest in luminal breast cancer via experimental in vitro, network pharmacology, and bioinformatics validation. In this research, MCF-7, a luminal model cell, was treated with galangin dose-dependent. Consequently, galangin exhibited a cytotoxic impact, with IC50 values of 117.86 μM. After that, SwissTargetPrediction, UALCAN, ShinyGO, and OncoLnc were used for bioinformatics validations, and Cytoscape software and the STRING website were used for computational analysis. Eight overlapping galangin target genes against luminal breast cancer were found. According to the analysis of the protein-protein interaction (PPI) network, eight hub genes-including CDK1, PLK1, TOP2A, ESR1, AURKB, NEK2, MMP9, and CA12-had the highest degree of freedom. Cell cycle regulation has been discovered to be tightly associated with overexpression of CDK1, PLK1, AURKB, and NEK2. By influencing the cell cycle, galangin inhibits the growth of luminal breast cancer, as determined by GO and KEGG enrichment analyses. In conclusion, by triggering cell cycle arrest, galangin may be used as a prospective chemotherapeutic treatment
Elevation Affects the Development, Growth, and Cocoon Quality of Eri Silkworm Samia cynthia ricini Boisduval (Lepidoptera: Saturniidae)
Full text linkSamia cynthia ricini is a species of silkworm whose survival, development, and cocoon quality are affected by its environmental factors such as elevation, which affects temperature and humidity. This study aimed to examine the impact of elevation on the survival, development time, cocoon shell weight, and fecundity of S. c. ricini. The research was performed at two sites with different elevations: highland and lowland regions. The findings indicated that S. c. ricini, reared in the highlands, had higher survival rates than those cultivated in the lowlands. Meanwhile, the developmental time of S. c. ricini reared in the lowlands was faster than in the highlands. Additionally, the cocoon shell weight of S. c. ricini reared in the lowlands was also heavier, and the total fecundity of the adults of S. c. ricini reared in the lowlands was higher than those reared in the highlands. This study recommends that S. c. ricini be reared in lowland settings for best results
Habitat Reduction and Population Structure of Endangered Asian Arowana (Scleropages formosus) Golden Red in Riau Sumatra, Indonesia
Full text linkThis research was conducted in the arowana golden red Conservation Area (AgrCA) in Riau Province Rokan Hulu Regency, Sumatra, Indonesia from 2020 to 2023. The primary objective was to elucidate the population structure of arowana golden red in the midst of ongoing habitat degradation resulting from land use changes in AgrCA. Land use changes were analyzed by satellite imagery through the QGIS application. We collected population sampling data for arowana golden red by using \u27fish net\u27, \u27fish trap\u27, and \u27fish scoop-net\u27. A comparison of satellite images taken in 2017 and 2021 revealed a reduction in swamp area by 664 ha (from 921 to 257 ha) and a decrease in swamp forest by 116 ha (from 264 to 148 ha). The conversion of swamp and swamp forests into oil palm plantations within the AgrCA has significantly diminished the natural habitat of the arowana golden red. Although there has been decreasing in swamp and swamp forests in AgrCA, the water quality in the AgrCA has remained suitable for the life of the arowana golden red. The population of arowana golden red was found to be 14-44 individuals year-1 in 2021-2023 with various sizes. Arowana golden red is most prevalent during the rainy season (July-December). Preserving the remaining swamp and swamp forests is paramount, representing a top priority. Immediate ex-situ conservation efforts are imperative, taking preemptive measures in case the arowana golden red can no longer inhabit its natural habitat