HAYATI Journal of Biosciences
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Metagenomic Analysis of Bacteria Phylum Firmicutes and Bacteroidetes in Women with Type 2 Diabetes
Get PDFDiabetes mellitus (DM) is a serious health problem in Indonesia. Type 2 diabetes is a metabolic disease which primarily caused by obesity-linked insulin resistance. There is a link between insulin resistance and bacterial populations in the gut. Therefore, the aims of this study was to analyze composition of bacteria belong to the phylum Firmicutes and Bacteroidetes in women with type 2 diabetes and non-diabetic persons as control. The study included five female adults type 2 diabetes patients and five healthy controls. The bacteria composition was analyzed for abundance by denaturing gradient gel electrophoresis (DGGE). Metagenomic analysis based on 16S rRNA gene was represented by 12 DGGE bands. The twelve respective bands showed the similarity ranging from 77 up to 98%. Diversity of microbial composition in women type 2 diabetes was represented by five DGGE bands are Bacteroides fragilis, Bacteroides vulgatus, uncultured Bacteroides sp. clone Lb4eF4, uncultured bacterium clone 16sms90-5g05, and uncultured bacterium clone Malaga 1F14. Four DGGE bands in women healthy controls are closely related to uncultured bacterium isolate DGGE gel band Eub42, uncultured bacterium isolate DGGE gel band K115, uncultured bacterium clone HFV04255, and Enterococcus sp. the finding shows bacterial species belong to the phylum Bacteroidetes was mostly found in women diabetic group, while those belong to the phylum Firmicutes was mostly found in healthy controls
Morpho-Agronomical Diversity of Forest Clove in Moluccas, Indonesia
Get PDFForest clove (Zyzygium aromaticum) is a type of wild clove found in Moluccas. The increasing interest of farmers in cultivating this plant leads to complete their information of morpho-agronomical characteristics which could provide diversity reflecting morpho-agronomical their distribution areas. This study aimed to characterize the morpho-agronomical traits of Forest clove plants in Moluccas. By survey, Forest clove more than 15 year old tree totalling of 50 populations were observed their 54 morpho-agronomical characteristics in two areas (Ambon and Seram) from March to June 2018. Two aromatic cloves, namely Tuni and Zanzibar were used for comparative analysis. The results showed that Forest cloves had a similarity of 78% among the population and grouped into 3 groups with a morpho-agronomical variation of 22%. On the contrary, Forest cloves and comparators (Tuni and Zanzibar) had morpho-agronomical differences of 58%. Based on the main component analysis, there were 11 most influential characters of Forest cloves (leaf size index, leaf length, leaf width, leaf area, petiole length, flower length, diameter of flower tube, ripe flower weight, fruit length, fruit width, and fruit weight) which could be descriptors for this plant species
Cytotoxic Activity of Alpinumisoflavone from Erythrina poeppigiana (Leguminosae) Against Colon Cancer (WiDr), Cervical Cancer (Hela), and Hepatoma Cancer (HepG2) Cells
Get PDFCancer is the second cause of death after cardiovascular diseases in the world. Anticancer prevention used can cause undesirable things. Flavonoids are secondary metabolites derived from natural products that are useful for anticancer treatment. This study was performed to observe the cytotoxic activity of alpinumisoflavone from Erythrina poeppigiana, toward cervical cancer (Hela), colon cancer (WiDr), and hepatoma cancer (HepG2) cells. The cytotoxic activity of alpinumisoflavone was tested using (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) assay. The percentage of cell mortality was calculated and the IC50 was calculated using probit analysis. The result shown that alpinumisoflavone has antiproliferative effect to colon cancer (WiDr), cervical cancer (Hela), and hepatoma cancer (HepG2) cells with the value of IC50 are 5.63, 7.18, and 18.08 µg/ml, respectively. Based on the value of IC50 alpinumisoflavone is very cytotoxic to colon cancer WiDr cell
Monitoring Sugarcane Growth Phases Based on Satellite Image Analysis (A Case Study in Indramayu and its Surrounding, West Java, Indonesia)
Get PDFThis study is intended to examine the growing phases and the harvest of sugarcane crops. The growing phases is analyzed with remote sensing approaches. The remote sensing data employed is Landsat 8. The vegetation indices of Normalized Difference Vegetation Index (NDVI) and Enhanced Normalized Difference Vegetation Index (ENDVI) are employed to analyze the growing phases and the harvest of sugarcane crops. Field survey was conducted in March and August 2017. The research results shows that March is the peak of the third phase (Stem elonging phase or grand growth phase), the period from May to July is the fourth phase (maturing or ripening phase), and the period from August to October is the peak of harvest. In January, the sugarcane crops begin to grow and some sugarcane crops enter the third phase again. The research results also found the sugarcane plants that do not grow well near the oil and gas field. This condition is estimated due as the impact of hydrocarbon microseepage. The benefit of this research is to identify the sugarcane growth cycle and harvest. Having knowing this, it will be easier to plan the seed development and crops transport
Ecology and Conservation of the Endangered Banteng (Bos javanicus) in Indonesia Tropical Lowland Forest
Get PDFBanteng, Bos javanicus, as wild cattle is a vital and importance source of germplasm in Indonesia. Various human activities currently threaten their conservation status. Nonetheless, no long-term monitoring programmes are in place for this species. Using distribution point and statistical analysis based on 46,116 camera trap days from December 2015 to January 2017, we aimed to provide habitat preferences, activity patterns and ecological data for banteng population in Ujung Kulon National Park (UKNP). It is the largest population of banteng in Indonesia and is living in a limited habitat area. According to the best occupancy model, the most suitable areas for this species were the secondary forest located at the center portion of UKNP. The presence of the invasive cluster sugar palm, Arenga obtusifolia, in dry season provides additional alternative food for banteng when its main food is scarcer in the forest. Banteng was cathemeral all year round, with the proportion of cathemeral records and the recording rate did not change with the protection of the level area, moon phase or season. To reduce the probability of encountering predators, banteng avoided the space use of dholes. Selection and avoidance of habitats was stronger than avoidance of the predator activity areas. Habitat competition from domestic cattle which grazed illegally in the national park appears to be a problem to the species since zoonosis appears from domestic cattle to banteng. Therefore, effective law enforcement and an adequate conservation strategy are required to eliminate the impacts of both direct and indirect threats
Tropical Peatland Identification using L-Band Full Polarimetric Synthetic Aperture Radar (SAR) Imagery (Study Case: Siak Regency, Riau Province)
Get PDFFrom previous research reported that tropical peatland is one of terrestrial carbon storage in Earth, and has contribution to climate change. Synthetic Aperture Radar (SAR) is one of remote sensing technology which is more efcient than optical remote sensing. Its ability to penetrate cloud makes it useful to monitor tropical environment. This research is conducted in a tropical peatland in Siak Regency, Riau Province. This research was conducted to identify tropical peatland in Siak Regency using polarimetric decomposition, unsupervised classifcation ISODATA, and Radar Vegetation Index (RVI) from SAR data that had been geometrically and radiometrically corrected. Polarimetric decomposition Freeman-Durden was performed to analyze radar backscattering mechanism in tropical peatland, which shows that volume and surface scattering was dominant because of the presence of vegetation and open area. Unsupervised classifcation ISODATA was then performed to extract “shrub class”. By assessing its accuracy, the class that represents shrub class in reference map was selected as the selected “shrub class”. RVI then was calculated using a certain formula. Spatial analysis was then conducted to acquire certain information that average value of RVI in tropical peatland tend to be higher than in non-tropical peatland. By integrating selected “shrub class” and RVI, peat classes were extracted. The best peat class was selected by comparing with peatland referenced map which is acquired from the Indonesian Agency for Agricultural Resources and Development (IAARD) using error matrix. In this research, the best peat class yielded 73.5 percent of Producer’s Accuracy (PA), 81.6 percent of User’s Accuracy (UA), 66.1 percent of Overall Accuracy (OA), and 0.1079 of Kappa coefcient (Ks)
Identification of Banana Plants from Unmanned Aerial Vehicles (UAV) Photos Using Object Based Image Analysis (OBIA) Method (A Case Study in Sayang Village, Jatinangor District, West Java)
Get PDF Banana is one of the leading fruit commodities of Indonesia and ranks the sixth position as one of the largest banana producers in the world. There are more than 200 types of banana in Indonesia. The utilization of bananas is influenced by the local culture, where in every 10 horticultural households, 5 of them plant bananas both as garden plants or field plants. This horticultural crop is expectantly being one of the actions to improve economic prosperity especially in rural areas. In maintaining the diversity of the growing bananas in rural areas, a geospatial approach to identify the vegetation is required. Remote sensing technology is one of the solutions to observe and to develop banana plants with one of the methods namely Object Based Image Analysis (OBIA). This method consists of segmentation, classification, and validation. In classification process, the OBIA method distinguishes objects not only based on pixel values but also on the basis of the shape, area, and texture around them. This research has proven that the classification using OBIA method is better than the traditional classification such as maximum likelihood classification method to identify banana plants. OBIA method can quickly identifies the vegetation and non-vegetation, also the regular plants and banana plants
Ground Vegetation Diversity on Different Type of Riverbank Along Ciliwung River in Bogor City, West Java
Get PDFCiliwung riverbank has been affected by settlement occupation. Both concrete and another retaining wall system have been built to avoid flood and erosion to the settlement that has existed adjacent to the river. Built environment as human disturbance can trigger the change of species richness of ground vegetation in the Ciliwung riverbank of Bogor City. The research objective is to study the difference of ground vegetation biodiversity in three different types of riverbank based on its condition: natural, semi-natural, and constructed riverbanks. Point method and photographic sampling are used. Five replications of a square metre plot at three transects were placed at four sites sampling along 250 m each from the three different types of riverbank. Images of the plots are trained using sample point to overcome the difficulties in the field observation and statistical analysis. The result discovered that species richness in the natural riverbank is significantly higher than in semi-natural and constructed riverbank. Yet, no significance of species richness is shown between semi-natural and constructed riverbank. Total species of ground vegetation that we found were 55 species. Each different type of riverbank has its own highest species coverage: Asystasia nemorum (55.00%) in the natural riverbank, Pilea nummulariifolia (33.07%) in the semi-natural riverbank, and Pogonatherum crinitum (52.80%) in the constructed riverbank. The study concluded that the construction in the riverbank largely altered the biodiversity of ground vegetation in the urban riverbank. The outcomes suggest that the remnant of natural riverbank in the urban landscape should be highly protected from urban development to preserve its biodiversity. Also, species found could be potentially developed as landscape plants to support urban river planning, design, and management
Androgynomonoecious Jatropha curcas: Chromosomes, Isozymes, and Flowers Gender
Get PDFJatropha curcas (J. curcas) is usually monoecious plants, which have male and female flowers on the same inflorescence. However, J. curcas can be found as an androgynomonoecious plant (have male, female, and hermaphrodite flowers), even though very rare. Androgynomonoecious J. curcas can be identified after six months of planting when it had started flowering. Therefore, it is important to identify the characteristics of androgynomonoecious J. curcas that can differentiate between androgynomonoecious and monoecious plants in earlier stages of growth. The objectives of the research were to observe isozymes, chromosome and flowers gender of androgynomonoecious and monoecious J. curcas Banten and Lampung accessions. Seeds from five genotypes of J. curcas were used in the research. The observation was carried out on the chromosome and isozymes (Peroxidase and Esterase isozymes) could be used as markers to differentiate androgynomonoecious and monoecious plants. Observations about the flower gender from offsprings derived from different seeds were important to know the inheritance of flower gender. The androgynomonoecious and monoecious J. curcas were diploid with number of chromosomes 2n=2x=22. The chromosomes of androgynomonoecious have longer than that of monoecious J. curcas. The isozymes of androgynomonoecious J. curcas had four alleles and monoecious J. curcas (Banten female monoecious) had three alleles. The flower inflorescence and gender derived from androgynomonoecious plants were unstable, due to androgynomonoecious is intermediate state
Propagation of Dendrobium antennatum Lindl via Seed Culture In Vitro Using Simple Medium: Fertilizer and Complex Organic Based Medium
Get PDFA New Guinea Orchid, Dendrobium antennatum Lindl urgently needs to conserve. The availability of in vitro germination technique for this orchids is significantly important to present the useful tool for conservation effort. This study aimed to find a simple media that consists of Growmore 10-55-10, a foliar fertilizer and complex organic i.e. coconut water, banana pulp, onion spring extract, and carrot juice. Five in vitro medium for seed germination and four in vitro sub-culture medium for planlet growth were formulated and used in this study. Our findings revealed that seed germination in vitro of Dendrobium antennatum well occured on the medium of Growmore 10-55-10 supplemented with 10% coconut water (GCw) and of Growmore 10-55-10 supplemented with 50 g/l extract spring onion. The seed germination process from sowing the seed to plantlet production ready to transfer to sub-culture medium for further growth took time 140 days. The embryo became pale green at the day 11 on the germination media. It could be used as a tool for detection of viability of the seeds. Both seed germination medium could be used also as sub-culture medium for enhancing the growth of the plantlets but not for multiplication of shoots