HAYATI Journal of Biosciences
Not a member yet
1069 research outputs found
Sort by
Comparison Between the Potential of Tempe Flour Made from Germinated and Nongerminated Soybeans in Preventing Diabetes Mellitus
Get PDFThis study was aimed to compare the chemical characteristics of tempe flour made from nongerminated soybean (NST) and germinated soybean (GST), especially on their capacity in preventing diabetes mellitus (DM). Soybeans were germinated for 20 hours in the dark until 2.5-5.0 mm of the radicle emerged. The ungerminated soybeans and the germinated soybeans were then processed into tempe and tempe flour. The two types of tempe flour were subjected to proximate analysis, amino acid profiling, antioxidant capacity, total phenol content, isoflavone content, and α-amylase and α-glucosidase enzyme inhibition analyses. GST was superior in preventing DM in the protein content and antioxidant parameters, as these were significantly higher (p<0.05) than in NST. On the other hand, NST was superior in preventing diabetes in the isoflavon (daidzein, genistein, and total isoflavone) and α-amylase inhibition IC50 parameters which were significantly better (p<0.05) than in GST. On the contrary, the diabetes-preventing parameters total phenols, α-glucosidase inhibition IC50, and insulinotropic amino acids (arginine, alanine, phenilalanine, isoleucine, leucine, and lysine) were not different (p>0.05). Therefore, GST and NST both have potential in preventing diabetes through different mechanisms
Rhizopus Rotting on Agricultural Products in Jakarta
Get PDFAgricultural products may easily perish if they are not well taken care of during postharvest treatment. One of the main causes in damaged products is the biological contamination of pathogenic fungi, such as Rhizopus spp. that gives rise to Rhizopus rot symptoms. The purpose of this research was to isolate Rhizopus spp. from different agricultural products showing Rhizopus rot symptoms as well as to identify them. Rhizopus pure cultures were isolated on potato dextrose agar. Identification was done through molecular techniques using PhytopureTM DNA Extraction Kit and Maxwell® RSC Plant DNA Kit for DNA isolation, internal transcribed spacer (ITS4 and ITS5) as primers for amplification, and molecular evolutionary genetics analysis 7 (MEGA7) for the reconstruction of phylogenetic tree from sequence result. The phylogenetic tree using Maximum Likelihood statistics with 1,000 replications of bootstrap test showed five strains, namely AR9, AR10, AR11, AR13, and AR14, which belong to R. delemar, and the other seven remaining strains, AR1-AR7 belong to R. stolonifer. Identification was clarified with morphological and physiological data using Rhizopus growth at temperature control of 33 and 42°C as well as microscopic observation involving rhizoid, columella, measurement of sporangiospores, and sporangiophores
Ecological Vulnerability of Coral Reef Ecosystem in Wakatobi National Park During Indian Ocean Dipole Event
Get PDFThis research examines coral reefs vulnerability which threatening its existences and functions by climate change. The ecological vulnerability in Wakatobi (Wangi-wangi, Kaledupa, Tomia, and Binongko) was assessed during Indian Ocean Dipole (IOD) event in 2016. Climate exposure was determined using sea surface temperature, chlorophyll-a concentration, and wind speed magnitude; sensitivity was determined using coral susceptibility, fish susceptibility, and macroalgae primary productivity rate; then adaptive capacity was developed by hard coral cover, coral size distribution, coral richness, fish biomass, herbivore diversity, and herbivore grazing relative to algal production. The values of Exposure, Sensitivity, and Adaptive capacity in Wakatobi were 0.93±0.02, 0.42±0.18, and 0.44±0.10, respectively. Site specific vulnerability scores ranged from 0.52 to 1.60 (mean 0.92±0.26). Binongko was the least vulnerable than other islands. Tomia was observed as the least adaptive capacity and Wangi-wangi was the most bleaching incidents. These results could help coral reefs monitoring priority during the event and then when the event is gone by focusing on the marked islands and sites. Sites that were observed as more vulnerable is urgently need a management strategy to overcome the vulnerability status in the future, such as increasing site adaptability
Flight Activities and Pollen Load of Lepidotrigona terminata Smith (Apidae: Meliponinae)
Get PDFWorker bees actively forage to supply colony necessity i.e., pollens, nectar, and resin. Flight activities of the worker bees are influenced by food availability and environmental conditions. This study aimed to measure flight activities of Lepidotrigona terminata in terms of leaving and returning to the nest (including carrying of pollen and pollen type) and their relation to environmental conditions. The observation of flight activities of L. terminata were conducted from August to December 2016, at 07.00-17.00 for 1 minute with 1 hour interval. Pollen load and pollen types were analyzed by the acetolysis method. Flight activities of leaving and returning to the nest were lower at 07.00-08.00 (1 individual/min) and at 16.00-17.00 (2 individuals/min). However, the peak activities occurred at 10.00 until 13.00 (8 and 6 individuals/min for leaving and returning to the nest, respectively). Temperature and light intensity were positively correlated with flight activities of bees that carried nectar and resin, as well as leaving the nest without garbage. The returned workers averagely carried 32,696 pollen grains from four pollen types. Based on selection index values, this stingless bee species mostly preferred Araceae (ⱳi=1.522) plant family for pollen resources to others, Anacardiaceae, Aceraceae, and Acanthaceae (x2=39.32, p<0.01)
Screening of Endophytic Bacteria as Biocontrol Agents Against Bacteria Leaf Blight (Xanthomonas oryzae)
Get PDFBacterial leaf blight (BLB) is one of major threats in rice production as it can cause 100% yield loss. Concern on the environment and human health has led an attempt to replace existing methods of chemical control and avoid extensive use of bactericides by using endophytic bacteria. The present study was conducted to screen and characterize bacteria isolated from different sources that has potential as antagonistic bacteria against Xanthomonas oryzaepv. oryzae (Xoo), the causal agent of bacterial leaf blight of paddy. Two hundred and thirty-three endophytic bacteria were successfully isolated from roots and leaves from paddy field. Only 17 endophytic bacterial isolates showed positive antagonistic activity indicated by inhibition zone around bacterial colony against Xoo on nutrient agar plate with 2 endophytic isolates (BCA 3 and BCA 12) showed highest inhibitory effect with 35±0.00 mm in diameter. Molecular identification by 16S rRNA amplification successfully identified the antagonistic endophytic bacteria as Pseudomonas fluorescensand Geobacillus thermoparaffinivorans. Findings in this study revealed the biocontrol abilities of isolated endophytes as an excellent option to be used by agriculture sectors to have sustainable environment
Genetic Relationship between Tongka Langit Bananas (Musa troglodytarum L.) from Galunggung and Maluku, Indonesia, Based on ITS2
Get PDFTongka Langit or Fe’i banana (Musa troglodytarum L.) has the T genome and a very high content of beta-carotene. It only grew and spread around the regions of Maluku islands and Papua. However, recently our team found this banana on the foot of mount Galunggung, West Java, so this raised the question about its origin. The objective of this study was to understand the genetic relationship between Tongka Langit from Galunggung and Maluku islands and compared it with other bananas with different genomes. Genetic diversity analysis was done using ITS2 DNA marker and dendrogram analysis showed three groups. From the comparison of the ITS2 sequences, there were no difference (100% identity) between the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. In conclusion, based on the ITS2 marker, the Tongka Langit were more distantly related to cultivars with A and B genomes, and there was no difference in the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. To the best of our knowledge, there is no previous report of genetic relationship between Tongka Langit from Galunggung and other regions
Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium
Get PDFParthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst
In silico Vaccine Design against Dengue Virus Type 2 Envelope Glycoprotein
Get PDFDengue fever is caused by the mosquito-borne virus termed (DENV). However, DENV-2 has been identified as the most prevalent amongst the Indonesian pediatric urban population, in contrast with the other four serotypes. Therefore, it is important to reduce severe infection risk by adopting preventive measures, including through vaccine development. The aim of this study, therefore is to use various in silico tools in the design of epitope-based peptide vaccines (T-cell and B-cell types), based on the DENV-2 envelope glycoprotein sequences available. Therefore, in silico methods were adopted in the analysis of the retrieved protein sequences. This technique was required to determine the most immunogenic protein, and is achieved through conservancy analysis, epitope identification, molecular simulation, and allergenicity assessment. Furthermore, B4XPM1, and KAWLVHRQW were identified from positions 204-212, while the 77 to 85 peptide region was considered the most potent T-cell and B-cell epitopes. The interaction between KAWLVHRQW and HLA-C*12:03 occurs with maximum population coverage, alongside high conservancy (96.98%) and binding affinity. These results indicated a potential for the designed epitopes to demonstrate high immunity against DENV-2
Peptides Hydrolysate Derived from Collagen of Snakehead Murrel (Channa striata) Skin Demonstrate Anti-cholesterol and Anti-oxidant activities
Get PDFAnti-cholesterol and anti-oxidant play a crucial role to combat cardiovascular disease (CVD), due to formation of arterial plagues from oxidation of cholesterol. In the past decades, bioactive peptides demonstrating anti-cholesterol and anti-oxidant activities have emerged as the alternative drugs. In this study, acid soluble collagen was extracted from the skin of snakehead murrel and employed to induce secretion of collagenase by Bacillus licheniformis F11.4. The collagenases secreted were in turn used to produce peptides hydrolysate and were grouped in two distinct collagenase fractions, designated as fraction D and F. Peptides hydrolysate produced by the fraction D was found to demonstrate HMG-CoA inhibitor activity comparable to pravastatin and limited anti-oxidant activity. Meanwhile, peptides hydrolysate generated using the fraction F demonstrated anti-oxidant activity comparable to BHT (2mM), vitamin C (2mM), and vitamin E (2mM), but limited HMG-CoA activity. Combination of the fraction D and F resulted in substantial HMG-CoA inhibition and anti-oxidant activities
Soil Moisture Mapping at Paddy Field in Indramayu Residence Using Landsat 8 OLI/TIRS
Get PDFDrought monitoring is important for the paddy planting planning. Remote sensing is one tool can be used for it. Paddy field monitoring based on the soil moisture gives much knowledge related to the water content in the soil. Soil moisture analysis in this study is using Normalized Different Water Index (NDWI), Linear Soil Moisture (LSM), and Tasseled Cap. Soil moisture change could explain based on calculation results of NDWI, Linear Soil Moisture (LSM), and Tasseled Cap Transformation (TCT). Based on the results has explained that the driest year occurs in 2015 and June 2016 has a higher soil moisture. Comparison with the radar shows that the results of soil moisture analysis with Landsat was effective can be used with results relatively close to the radar results