HAYATI Journal of Biosciences
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The Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B Receptor Expression in the Liver Diabetic Rats
No full textUncontrolled diabetes mellitus (DM) has been linked to depression and lipid metabolism impairment. Serotonin (5-HT) has been known to improve lipid metabolism and affect mood. The 5-Hydroxytryptophan (5-HTP) in banana peel flour (BPF) is a precursor to synthesize 5-HT in the body, which is linked to initiating liver regeneration via HTR2B receptors. The impact of 5-HTP in the diet on HTR2B receptor expression has yet to be thoroughly investigated. We aimed to elucidate the correlation between serum lipid profile and immobility time in Tail Suspension Test (TST) as depressive-like behavior and compare the expression of HTR2B receptor in healthy and diabetic rats. Male Wistar (Rattus norvegicus) rats were assigned to the control and treatment groups in a DM model with streptozotocin (60 mg/kg) injected intraperitoneally, then either fed standard diet or BPF 10% supplemented standard diet for 21 days. Immunohistochemical staining was used to examine the expression of 5-HT, HTR2B receptors. We determined that diabetic rats fed the standard diet supplemented 10% BPF group showed significantly lower concentrations of total cholesterol and triglycerides compared to diabetic rats fed a standard diet and it was positively correlated between total cholesterol and LDL with the duration of immobility time in TST. The average immunoreactivity score in diabetic rats fed 10% BPF was the highest among the other groups, indicating that the available BPF dose is sufficient for HTR2B activation, which will support the liver cell regeneration process, and should be investigated further
Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance
Get PDFThis study was intended to obtain white rust (Puccinia horiana) disease resistance Chrysanthemum transformed with wasabi defensin gene through mediation of Agrobacterium tumefaciens from three explant sources, i.e., leaf, lateral shoot bud, and internode. Observations were made on transformation efficiency, PCR analysis, in vitro and ex vitro disease resistance tests. Results showed that efficiency of transgenic callus and shoot regeneration was found both highest from lateral shoot buds (57.5% and 50.0%, respectively). PCR analysis showed that three putative transgenic plantlets from lateral shoot buds and one from leaf explant were putative transgenic carrying the wasabi, hpt, and nptII genes. Rooting test showed that the highest number of rooted plants was found in treatment of hygromycin (Hg) 25 mg L-1 (81%) and lowest was in treatment combination of kanamycin (Km) 50 mg L-1 + Hg 25 mg L-1 (25%). In vitro disease resistance test with sorus inoculation of P. horiana, directly on the leaves, resulted in 20 resistant plants out of 30 putative transgenic plants (66.67%). Ex vitro testing on adult plants of the same samples in a confined closed greenhouse (CGH) resulted in average of 80% transgenic Chrysanthemum plants were resistant, whereas in control plants caused white rust disease symptom
Overproduction, Purification and Refolding of codon-optimized Hepatitis B Virus X Protein Subgenotype B3 in Escherichia coli BL21(DE3)
Get PDFHepatitis B virus (HBV) infects human and causes chronic liver infection, leading to liver cirrhosis and hepatocellular carcinoma. HBV X (Hbx) protein is known to interact with tumor suppressor protein p53 and block its translocation into the nucleus. This study outlines the overproduction of Hbx protein from HBV subgenotype B3 in Escherichia coli BL21(DE3), including its purification and refolding. The gene encoding Hbx was first codon-optimized and inserted into pET16b. The recombinant plasmid was then transformed into E. coli BL21(DE3) as an expression host. Optimization of Hbx expression was performed with variation of IPTG concentration and overproduction temperature. The results showed that Hbx protein was optimally induced by 0.075 mM IPTG and overproduction of Hbx at 17, 25, and 37°C exhibited no difference in protein level and location. The optimal refolding of Hbx was obtained using 0.1 M arginine prior to elution from Nickel column using 100 mM imidazole and 0.25 M arginine. Hbx migrates differently in SDS-PAGE reducing and non-reducing, while the melting curve pattern in TSA analysis changed after the refolding step. Essentially, this purified Hbx protein could potentially be used for interaction study with p53 and the inhibitor candidate of the protein
Evaluation of Cardiovascular Biomarkers and Lipid Regulation in Lactation Friesian Holstein at Different Altitude in West Java, Indonesia
Get PDFAltitude contributes and plays an important role in the development dairy cows in Indonesia. Altitude is directly related to temperature and humidity, both of which affect cardiovascular function and energy metabolism in dairy cattle. The 120 samples, consisted of 4th-5th lactation Friesian Holstein dairy cows were used in this study. The 40 samples of cattle each spread in three maintenance sites with different altitudes, namely 300 to 500 m above sea level (a.s.l.); 600-900 m a.s.l. and >1,000 m a.s.l. All the study sites located in West Java, Indonesia, to study the effect of altitude on the cardiovascular biomarker and lipid regulation in the dairy cow. Based on the result in this experiment showed that the CRP high sensitivity, H-FABP, homocysteine, and γ-Glutamyl Transpeptidase in Frisian Holstein dairy cows expressed higher levels (P <0.05) at low altitude site (300-500 m a.s.l.) than dairy cows at altitude sites higher (600-900 and >1,000 m a.s.l.), These results indicated, these compounds can be biomarkers for cardiovascular function. This study also showed, the lipid regulation also showed higher levels (P <0.05) at low altitude sites than altitude sites higher
Pollination by Three Species of Stingless Bees (Hymenoptera: Meliponini) Increase Seed Set of Mustard (Brassica rapa L.: Brassicaceae)
Get PDFPollination and fertilization are critical factors in horticultural cultivation to increase seed production. In this study, we measured the visiting activity and pollination of three species of stingless bees, i.e., Tetragonula laeviceps, Lepidotrigona terminata, and Heterotrigona itama in mustard (Brassica rapa). Visiting activity of stingless bees were observed in mustard plants caged by an insect screen. The observations were done visually per hour, starting from 07:00 until 16:00, using the focal sampling method. The visiting activities observed were flower handling time, foraging rate, and total visit per plant. We also measured the pollen load of each stingless bee species. Results showed the highest foraging rate occurred in H. itama (28.38 flowers/5 minutes), followed by T. laeviceps (27.88 flowers/5 minutes), and L. terminata (27.85 flowers/5 minutes), respectively. The longest flower handling time was found in H. itama (40.15 seconds), followed by T. laeviceps (35.25 seconds), and H. terminata (34.84 seconds). Whereas, the highest total visiting time occurred in H. itama (40.10 minutes), followed by T. laeviceps (37.47 minutes), and L. terminata (36.76 minutes). Heterotrigona itama has a highest pollen load (36,650 pollen grains), followed by L. terminata (26,940 pollen grains), and T. laeviceps (9,700 pollen grains), respectively. Pollination by the stingless bees increase 27-40% the number of pods per plant, 32-72% the number of seeds per pod, 32-54% of seed weight per pod, and 18-36% of seed germination of mustard
Effect of Vitamin D3 Supplement on the Semen Quality in Human Patients with Vitamin D Deficiency
Get PDFMale fertility is a complicated process that can be hampered by a variety of lifestyle factors. This study aims to evaluate the effect of vitamin D3 deficiency on the semen quality and also to study the quality changes in the semen of patients with vitamin D3 deficiency, after supplementation with vitamin D3 for a month. This present study included 112 participants, who were classified into two groups according to the serum level of vitamin D3 (normal and deficiency groups). The semen was analyzed by the physical examination of both groups; also this study included follow-up patients with vitamin D3 deficiency, who were supplied with 5,000 IU of vitamin D3. The results showed a significant decrease in the motility of sperms in patients with vitamin D3 deficiency (27.16%±2.497%) compared to the standard group (36.71%±2.892%), and an increase in sperm motility in patients who consumed vitamin D3, after being followed up for a month (37.19%±2.836%). Moreover, the result showed a high and significant increase in the rate of deformities of sperms in patients with vitamin D3 deficiency (18.67%±1.074%) compared to the standard group (13.68%±0.8131%) and a decrease in the rate of deformities of sperms in patients who took vitamin D3 supplement for a month (13.54%±0.8116%). The study concluded that patients with a supplement intake of vitamin D3 had an effect on the semen quality, especially the motility and deformity of sperms in vitamin D3-insufficient men
Conjugational Transformation of Wild Type Bacillus halodurans CM1 by Methylated Recombinant Plasmid Harbouring a Gene Encoding for Alkaline Protease and the Protease Activity Assay of the Transformant
Get PDFProtease contributes significantly to various industrial sectors, as shown by its increasing demand. An indigenous previously isolated bacteria, Bacillus halodurans CM1, is a wild-type bacterium whose ability to produce alkalotermophilic protease. In this study, the transformation of methylated pBBRE194 plasmid containing alkaline protease gene from this strain (PBBRE194-prot-CM1) into itself using a conjugation approach was conducted, and the measurement of the alkaline protease activity of the recombinant bacterium was carried out. The recombinant B. halodurans CM1 has been verified to carry the methylated pBBRE194 prot-CM1 by examining its ability to degrade protein in media containing skim milk and tetracycline, but also by amplifying tetracycline resistance gene sequence with 1,024 bp length of plasmid pBBRE194 prot-CM1 by PCR method from the recombinant bacterium. The results confirmed that recombinant B. halodurans CM1 was positively harboring plasmid pBBRE194 prot-CM1. Alkaline protease produced by recombinant CM1 reached higher activity than wild type between 18-36 h of cultivation. The alkalotermophilic wild type B. halodurans could accept the recombinant plasmid into their cells via conjugational transformation is firstly reported to our further knowledge
The Effect of Cang Salak Tea Diet on Apolipoprotein C3 (ApoC3) Gene Expression on Hyperlipidemic Rats Model
Get PDFCardiovascular disease (CVD) is the leading cause of global mortality and disability. Hyperlipidemia is a major risk factor for CVD that can be controlled through medical therapy, appropriate nutrition, and lifestyle. This study aimed to identify the cang salak tea diet’s effect on the ApoC3 gene expression in a hyperlipidemia rat. 18 male Wistar rats were divided equally into three groups. A high-fat-diet-induced two groups of rats, and one group was the control. Once hyperlipidemia had been achieved, one of the two groups was treated with the cang salak tea, and one group was given a standard diet for four weeks. Authenticated rat and liver tissue were collected as a source of RNA isolation. Isolated RNA was used as a reaction template for the relative quantitation qPCR using β-actin as the housekeeping gene. The ApoC3 gene was specifically amplified with a Tm value of 82.73°C, Cq 17-19, and produced a sigmoid curve. The relative expression level of the ApoC3 gene in hyperlipidemia rats fed with the cang salak tea diet was 0.46 times significantly lower than the control (1.17) and P2 (1.32) groups. These results indicate that the cang salak tea has antihyperlipidemic properties to reduce CVD risk
Zooplankton Composition in Super-Intensive Whiteleg Shrimp, Litopenaeus vannamei (Boone, 1931) Culture Tanks
Get PDFThis study aimed to determine the zooplankton species composition in super-intensive whiteleg shrimp, Litopenaeus vannamei (Boone, 1931) tanks. The research was conducted from January to May 2021 in Bac Lieu City, Bac Lieu province, Vietnam. Eleven sampling times were divided into two periods, the nursery phase (six times) and the grow-out phase (five times) of shrimp culture. The results showed that water quality parameters fluctuated dramatically during the culture period, in which some nutrient concentrations tended to increase at the end of the shrimp culture period. Nine zooplankton species were recorded, of which five species belonged to Protozoa, three Rotifera species, and one Copepoda species. The number of zooplankton species did not differ significantly among the sampling periods. Protozoa had the highest species composition and density during most of the shrimp culture period. Copepoda was only identified in the nursery stage of shrimp culture. The species component of zooplankton had a close positive correlation with temperature, but their abundance did not have a significant relationship with water quality parameters because each species was affected by the different water quality parameters. Zoothamnium sp. had significantly positive correlations with total ammonia nitrogen (TAN), nitrate (NO3)-, total phosphorus (TP), and total nitrogen (TN) concentrations. The rotifer Brachionus plicatilis had a strong relationship with TP content, whereas Dartintinnus alderae had a strong relationship with alkalinity. Protozoa dominance in shrimp tanks could affect shrimp growth, decreasing the economic efficiency of shrimp farming. Therefore, the results of this study contribute to water quality and natural food management to improve shrimp productivity
The Changes in Seed Germination Capacity, Seedling Growth, and Leaf Morphology of Ficus variegata Blume Influenced by Gamma-Ray Irradiation
Get PDFGamma irradiation treatments have commonly been applied to induce variation in plant genetic improvement programs. The improvement of nyawai (Ficus variegata Blume) is urgently needed mainly to break the seed dormancy, increase seedling growth, and induce variation for selecting desired traits. This research aimed to assess the effect of gamma irradiation doses on seed germination, seedling growth, and leaf morphology of Ficus variegate Blume. This experiment\u27s irradiation doses ranged from 5-240 Gy and were applied to seeds with varying levels of vigor based on the artificial seed aging process. The data displayed a positive relationship between low doses of gamma irradiation and the nyawai seed\u27s endurance. Low irradiation doses, 5 to 30 Gy, are deemed capable of promoting seedling development, leaf retention, and diversity in leaf structure. The higher irradiation doses than 30 Gy decreased seedling growth, leaf number, and seedling survival. In addition, these doses also affect some morphological changes, including seedling height and leaf length and width