Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
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HISTOMORPHOLOGY OF PANCREATIC TISSUE IN MEDAKA FISH (Oryzias javanicus): STUDY OF DIABETIC ANIMAL MODEL DEVELOPMENT
Full text linkThis study aimed to explore the histomorphology of the pancreatic tissue of medaka fish to develop medaka fish as a diabetic animal model. Adult medaka fish were obtained with a body length of 2.530.22 cm and a weight of 265.825 g. Medaka fish were fixed using Boiun's fixative solution. Macro anatomical observations of the pancreas were conducted using a stereomicroscope. Histological preparations were made according to the Hematoxylin-eosin staining standard procedure. Pancreatic histomorphology of medaka fish was carried out using a light microscope with 100x and 400x magnification. The macro anatomy observations of the pancreas showed the interconnection with several tissues, such as spleen, gallbladder, liver, fat, and intestines. The pancreas was located toward the liver, oversees the intestines, and covered with fat. Pancreatic histomorphological observations showed exocrine cells composed of serous pyramidal acinar cells and endocrine cells (islets of Langerhans). Intralobular and intercalated ducts (ducts of Boll) were composed of simple cuboidal epithelium. This study concluded that the pancreas organ in medaka fish is a single organ, and histomorphologically has both of exocrine and endocrine cells
CAPABILITY OF DIFFERENT BREEDS OF DONOR CATTLE TO PRODUCE EMBRYOS AT THE CIPELANG LIVESTOCK EMBRYO CENTER, BOGOR, WEST JAVA
Full text linkThe objective of this research was to investigate the capability of the donor cattle to produce embryos by comparing three cattle breeds, namely Bos taurus beef cattle (Limousin), Bos taurus dairy cattle (Friesian Holstein/FH), and Bos indicus beef cattle (Peranakan Ongole/PO). This research was observational method with in vivo embryo production method and a nonsurgical embryo collection method (flushing). The data collected were then processed using the one way ANOVA method. The results showed that the number of CL, response rate, total number of embryos, and recovery rate in PO cattle, FH cattle, and Limousin cattle did not differ significantly (P0.05). The appropriate number of embryos to be transferred and degenerative embryo were significantly different among PO cattle, FH cattle, and Limousin cattle (P0.05), while the unfertil ova, fertilization rate, and transferable embryo did not differ among cattle breed (P0.05). This research concluded that each breed of the donor cattle produced embryos with varies quality and the best quality of embryo production is found in Limousin cattle
EFFECTIVENESS OF PROBIOTIC AND DIGESTIVE ENZYMES IN FERMENTED DIET ON GROWTH, FAT AND CHOLESTEROL CONTENTS OF BROILER
Full text linkABSTRACTThe present study aims to determine the effect of fermented diet containing probiotics and digestive enzymes with different feed levels on growth, fat and cholesterol contents of broiler chickens. Evaluating the effectiveness of probiotics (P) and digestive enzymes (DE) in fermented diet on broiler was observed by 4 treatments: P0=control (fermented diet without P and ED) EP), P1(fermented diet contained 0.5% P only)), P2 (fermented diet contained 0.5% DE only), P3 (fermented diet contained 0.5% P and 1% DE) and P4 (fermented diet contained 1% P and 0.5% DE). A hundred DOCs of broiler chickens strain CP 707 were used (unsex) and it designed by a completely randomized design with 5 treatments, 4 replications and each replication consisted of 5 DOCs. The data obtained were analyzed by means of variance and Duncan's multiple range test. The results of present study showed that broiler fed on fermented diet containing P and/or ED with different levels had a significant effect (P0.05) on final body weight, weight gain, carcass weight, fat content on thigh muscle. Broiler fed on fermented diet contained 0.5% DE only (P2) resulted in better growth and weight gain than other treatments. However, fermented diet contained probiotics and digestive enzymes had no effect on fat and cholesterol contents in breast muscle. It was concluded that the addition of digestive enzymes in fermented diet formulated by local raw materials was very effective than probiotics in increasing body weight gain, carcass weight and prevented the increase in fat and cholesterol contents both in breast and thight muscles of broiler chicken
BANGKOK CHICKEN SPERM QUALITY DURING STORAGE USING KA-EN 3B EGG YOLK DILUENT SUPPLEMENTED WITH RESVERATROL
Full text linkThe objective of this study was to determine the effect of resveratrol supplementation in KA-EN 3B-egg yolk dilution on sperm quality of Bangkok chickens. The semen was collected from 2 adult male Bangkok chickens with an age of 12 months and a body weight of 2.5 kg. The collected semen was divided into 5 treatments with 5 replicates each. The treatments used were KA-EN 3B-egg yolk (KEY), KEY + 0.02% resveratrol, KEY + 0.04% resveratrol, KEY + 0.06% resveratrol, and KEY + 0.08% resveratrol. The parameters observed were sperm motility, viability, and abnormalities during 24 hours, 48 hours, and 72 hours of storage in the refrigerator. The results showed that resveratrol supplementation in the diluent had no effect (P0.05) on sperm motility, viability, and abnormalities during storage. This study concluded that supplementation of resveratrol in KA-EN 3B-egg yolk diluent did not affect the quality of Bangkok chicken sperm during storage
EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER
Full text linkThis study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that stored on liquid nitrogen was isolated using DNAzol reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene
EFFECT OF ETHANOL EXTRACT FROM MALACCA LEAVES (Phyllanthus emblica) ON THE SPERM QUALITY OF MICE
Full text linkThis study aims to analyze the effect of administering Malacca leaves (Phyllanthus emblica) extract on the sperm quality of mice. This leaf extract was administered orally using gavage method once daily for 21 days. There were four treatment groups: the control group (P0) received distilled water while the first (P1), second (P2), and third (P3) treatment groups received 300 mg, 600 mg, and 1200 mg of Malacca leaf ethanol extract/kg body weight, respectively. Each groups consisted of four mice and was evaluated for sperrm motility, viability, morphology, and concentration. The results showed that sperm motility, viability, morphology and sperm concentration tended to decrease after administration of Malacca leaf ethanol extract for all parameters, although there was no significant difference (P0.05). It was concluded that the administration of Malacca leaf ethanol extract tends to reduce the sperm quality of mice. __________________________________________________________
THE BIOLOGICAL FUNCTIONS OF IMMUNOGLOBULIN Y (IgY) MOLECULES IN AGAINST INFECTION OF Enterococcus faecalis ORIGIN OF RED TILAPIA
Full text linkRed tilapia (Oreochromis hybrid) is Indonesia's leading freshwater fishery commodity susceptible to streptococcal bacterial infection. Many studies have been conducted on various efforts to prevent and treat this disease, one of which uses the immunoglobulin Y (IgY) molecule from chicken egg yolk. This study aimed to observe the biological function of IgY against Enterococcus faecalis as a cause of streptococcal-like infection. The agglutinin function was conducted by observing the growth of Enterococcus faecalis in brain heart infusion (BHI) broth media which was added with IgY suspension. The function of inhibin was performed using a spectrophotometric method to measure the level of turbidity of the bacterial suspension inoculated with IgY suspension. The bactericidal potential through the complementary activation pathway for red tilapia serum was carried out using a scanning electron microscope (SEM) method to evaluate the topography of the bacterial cell wall. The results of the study can be concluded that IgY anti-Enterococcus faecalis has the potential as an agglutinin, inhibin, and bactericidal agent through its putative potential in complement activation in streptococcal bacterial infections in red tilapia commodities
CANINE LEPTOSPIROSIS IDENTIFICATION IN SMALL ANIMAL PRACTICE
Full text linkLeptospirosis is an infectious disease caused by bacteria from the genusLeptospirawith various serovars and often presents with a wide range of clinical signs from mild to severe conditions, particularly it presents with a mild fever to asymptomatic infection-causing animal death. The purpose of this study was to identify the characteristics of canine leptospirosis through laboratory examinations supported by medical record data in a small animal practice at the drh Cucu K. Sajuthi Joint Veterinary Practice, in Jakarta. The study was conducted on serum samples of sixteen dogs from drh Cucu K. Sajuthi Joint Veterinary Practice patients, which presented varied clinical signs of leptospirosis, and which had been accompanied by hematological examination, blood biochemistry, and Microscopic Agglutination Test (MAT) against various serovars such asBataviae,Icterohaemorrhagiae,andJavanica. The collected data was analyzed descriptively. The results showed that canine leptospirosis was more commonly derived fromBataviaeserovar and was accompanied by clinical signs such as vomiting, jaundice, anorexia, abdominal pain, lethargy, pale mucosal membrane, diarrhea, dehydration, dyspnea, and polyuria/polydipsia. A predisposition of gender and age was commonly found in male dogs less than 5 years old. Abnormalities found from hematological examination were leukocytosis, granulocytosis (neutrophilia), anemia, thrombocytopenia, and lymphopenia, while blood chemistry showed azotemia, an increase in liver enzymes (AST, ALT, ALP), hyperbilirubinemia, hyponatremia, hyperphosphatemia, and hypoalbuminemia
DATE EXTRACT SUPPLEMENTATION IN RINGER LACTATE-EGG YOLK EXTENDER ON POST-THAWING QUALITY OF PELUNG CHICKEN SPERMATOZOA
Full text linkThe research purpose was to evaluate the influence of date extract supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate (LREYS) on post-thawing quality of Pelung chicken spermatozoa. Completely randomized design with 5 treatments and 4 replications was used in this study. Semen was collected from three Pelung chickens once in three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of date extract supplementation that were 0% as control (LREYSDE0), 0.5% (LREYSDE1), 1% (LREYSDE2), 1.5% (LREYSDE3), and 2% (LREYSDE4). The post thawing liquid semen was observed for sperm motility, viability, plasma membrane integrity and acrosome integrity. The result showed that the motility, viability, membrane plasma integrity and acrosomal integrity of spermatozoa in LREYSE extender with 1.5% date extract supplementation (30.441.02%; 49.830.91%; 43.261.02%; and 45.330.72% respectively) was significantly higher (P0.05) than others treatment that were 0% (18.210.43%; 35.620.51%; 30.540.60%; and 31.490.71% respectively), 0.5% (20.450.72%; 40.720.87%; 36.810.55%; and 38.560.63% respectively), 1% (25.680.93%; 46.270.75%; 40.521.02%; and 42.831.09% respectively) and 2% (22.550.92%; 43,710,74%; 38,840,71%; and 41,390,86% respectively) of date extract supplementation. It is concluded that the 1.5% date extract supplementation in extender can maintain the best post-thawing sperm quality
THE EFFECTS OF SIMVASTATIN, ROSUVASTATIN, AND FENOFIBRATE ON THE BODY WEIGHT AND LIPID PROFILES OF FEMALE RATS TREATED WITH ORAL CONTRACEPTIVES AND A HIGH-FAT DIET
Full text linkThis study aimed to compare the effects of simvastatin, rosuvastatin, and fenofibrate therapies on the body weight and lipid profiles of the female rats receiving a combination of oral contraceptives (COC) and a high-fat diet (HFD). Twenty female Wistar rats (130-200 g) were divided equally into four groups. The rats received a standard diet for seven days, and their baseline lipid profiles were analyzed. All rats also received COC containing 15.1 g/kg levonorgestrel/3.1 g/kg estradiol with HFD for 60 days. Starting on day 31, the rats were given the respected treatment. The control group received the vehicle, whereas the others were treated with either simvastatin (2.1 mg/kg), rosuvastatin (0.5 mg/kg), or fenofibrate (8.2 mg/kg). Blood samples were taken on days 30 and 60. The results showed that the administration of COC+HFD and vehicle for 30 days increased the rats body weight and dyslipidemia, characterized by a significant decrease in Low-Density Lipoprotein (HDL) and an increase in total cholesterol (TC) and triglycerides (TG) levels (P0.05). Among the treatments, only fenofibrate was found to be able to prevent a significant weight gain in rats. Both fenofibrate and rosuvastatin inhibited a significant rise in TG and TC levels. Meanwhile, the simvastatin administration failed to do the same. Although statistically insignificant, all treatments increased rats HDL levels. Thus, it can be concluded fenofibrate was the most effective treatment among all in reducing weight gain and improving the lipid profile of dyslipidemic rats induced by oral contraceptives and a high-fat diet