Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
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EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT
Full text linkThe objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW
MORPHOFUNCTIONAL CHARACTERISTICS OF THE LOWER HINDLIMB AND FOOT MUSCLES OF BORNEAN ORANGUTAN (Pongo pygmaeus)
Full text linkOrangutan is a great ape that has anatomical and physiological aspects similar to those of gorillas and chimpanzees, although orangutan is more arboreal than other great apes. This research aims to study the morphofunctional characteristics of the lower hindlimb and foot muscles of Bornean orangutan and its relation to the type of locomotion and daily behavior. This research was conducted on the right leg of a male orangutan by observing the morphology of the lower hindlimb and foot muscles and observing the behavior of the Bornean orangutan through video observations. The results showed that digital flexor muscles are more developed than plantar flexor muscles as an adaptation towards arboreal activities that require finger flexor movements such as gripping tree branches. Orangutans have rudimentary hallux, which is related by the less developed intrinsic muscle and indicated by the way orangutan grips tree branches dominantly using digits II-V. At a certain time, male Bornean orangutans are active on terrestrial that causes the digital extensor muscles to have more developed as an adaptation to terrestrial activity. It can be concluded the morphology of the lower hindlimb and foot muscles is in accordance to its function and behavior adaptation in arboreal and terrestrial
THE EFFICACY OF AVOCADO, MUNG BEAN SPROUTS, AND HOLY BASIL HERB COMBINATION (JAMU ATOKE) ON THE HEALTH AND REPRODUCTIVITY OF ADULT FEMALE RATS
Full text linkThe purpose of this study was to examine the efficacy of a herbal combination of avocado, mung bean sprouts, and holy basil (Jamu ATOKE) in optimizing the reproductive health of adult female rats. Eighteen female Sprague Dawley rats aged 9-10 weeks old weighing around 180-250 g, and had not been pregnant previously were equally and randomly divided into 3 groups: one control group and two treatment groups (n= 6 rats per group). The treatment groups received Jamu ATOKE with a concentration of 2.5 and 5%, respectively. Jamu ATOKE was added to the drinking water and consumed by the rats for 30 days prior to pregnancy. Reproductivity (pregnancy success, estrogen and progesterone hormone concentrations), and health performance (body weight gain, food, and water consumption, motor activity, red blood cell (RBC) count, white blood cell (WBC) count and differentials, SGPT, SGOT, ureum, and creatinine) of rats were observed. The results showed that groups receiving the Jamu ATOKE had better reproductive and health performance compared to those of the control group. The administration of Jamu ATOKE can significantly increase the concentration of estrogen and progesterone in rats (P0.05). It can also improve the fertility and health of the female rats prior to pregnancy
PHYSIOLOGICAL RESPONSES OF FEMALE BEEF CATTLE AGAINST PEATLAND MICROCLIMATE STRESS IN CENTRAL KALIMANTAN
Full text linkThis study aimed to evaluate the physiological response of female beef cattle to peatland microclimate stress in Central Kalimantan. This study used direct observation on small holder beef cattle farm. Microclimate data on 41 units cattle barns and physiological parameters of female cattle were collected in the morning, at noon and in the afternoon. The physiological parameter measurements involved 215 female beef cattle, consisting of 119 Bali and 96 crossbred cattle with different physiological stages including pregnant cows, lactating cows, dry cows, heifers and calves. The microclimate condition within cattle barns on peatland of Central Kalimantan is not the comfort zone for beef cattle. It is characterized by high air temperature and relative humidity, and low wind speed, which result in high temperature humidity index (THI). The barn with gable roof type and asbestos materials gave the lowest THI. This Microclimate caused heat stress to beef cattle reared on the peatlands, indicated by the high rectal temperature, heart rate, respiratory rate, and heat tolerance coefficient, although it was still categorized as mild to moderate stress. Bali cattle showed better physiological responses to microclimate stress than crossbred cattle. Pregnant crossbred cows were the most susceptible to peatland microclimate stress
EVALUATION OF LAPAROTOMY SURGERY WOUND HEALING IN DOGS USING THERMOGRAPHIC ANALYSIS
Full text linkThis study aimed to develop the use of thermographic analysis to evaluate wound healing for laparotomy surgery in animals. The experiment used nine adult dogs 5 males and 4 females, bodyweight 5-10 kg) that had undergone the same general anesthesia and laparotomy procedures. Evaluation of wound healing was performed at 24, 48, and 72 hours after surgery and 7 days after operations using a digital thermal camera. During each thermographic evaluation period, blood samples were taken for analysis of total leukocytes and leukocyte differential. The results of thermal imaging were compared with the values of inflammatory cells and the clinical condition of wound healing. Comparison of thermographic analysis with inflammatory status was evaluated using the regression equation and showed a strong correlation coefficient (Y= -0.4847 + 40.14, R2= 0.867), that is, the higher the temperature, the lower the inflammation. The conclusion of study is that thermographic analysis can be used to evaluate wound healing of laparotomy surgery in dogs
THE REPRODUCTIVE CHARACTERISTICS OF GAYO BUFFALOES AS A LOCAL LIVESTOCK GENETIC RESOURCE IN THE MESIDAH SUBDISTRICT, BENER MERIAH REGENCY
Full text linkThis research aimed to identify the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency. There were three locations of the current study namely Perumpaken Benjadi, Wer Tingkem, and Simpur, which were determined using the purposivesampling technique. A survey was used as the research method to obtain primary data and secondary data. Primary data were collected throughdirect interviews with buffalo livestock owners. They were asked a set of questions that had been prepared in a questionnaire. Secondary datawere obtained from related agencies in Bener Meriah Regency. The number of respondents was 92 from four villages in the Mesidah Sub-district,Bener Meriah Regency. Breeders as respondents were selected using a purposive sampling method, with minimum requirements that they hadraised two buffaloes that had given birth more than once and that the breeders had at least four years of farming experience. The research datawere analyzed using descriptive statistic. The results showed that the Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, had reproductive characteristics that met the standards. This can be seen from the obtained data which were almost the same as the average age of the reproductive characteristics of buffaloes in Indonesia. Gayo buffaloes in the Mesidah Sub-district experienced the first estrus (puberty) at 33.83 months old, first mating at 34.61 months old, and first calving at 45.29 months. The average gestation period was 10.51 months, the calving interval was 15.5 months, and the average service period was 3.66 months, while maintenance management was carried out extensively. Based on the results, it can be concluded that the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, still meet the standards. The average reproductive characteristics of Gayo buffaloes are generally the same as the average characteristics of other buffaloes in Indonesia
GENETIC DIVERSITY OF IRAQI LOCAL GOAT BREEDS BY RAPD DNA MARKERS
Full text linkGenetic diversity of three Iraqi local goat breeds (Black, Hybrid, and Meriz) was studied by using random amplified polymorphic DNA(RAPD) markers. Fifteen primers were used, they generated a total of 259 amplification products, from which 142 bands were polymorphic,revealing 55.29% polymorphism. It was found that the mean value of Neis gene diversity (h) and Shannon's nformation index (I) were 0.1778 and 0.2546, respectively. According to the analysis of genetic distances among breeds, the highest genetic distance recorded between Black and Hybrid 0.5108, while the lowest genetic distance recorded between Hybrid and Meriz 0.1431. The phylogenetic dendrogram based on Neis genetic distance analysis was in agreement with their phenotypic similarities in which the tree separated the surveyed breeds into two clusters, the first one including Black goat breed, and the second cluster includes Hybrid and Meriz breeds. These results indicated that Black goat breed is most genetically distant from both Hybrid and Meriz breeds. Finally, the moderate value for both of polymorphism and genetic diversity which obtained from the present study indicate that these breeds are genetically different, which provide a good background about these breeds forgenetic improvement and conservation of our local goat breeds in the future
HISTOPATHOLOGICAL OVERVIEW OF NEW ZEALAND WHITE PSEUDO-PREGNANT RABBIT UTERUS AFTER OVARY TRANSPLANTATION WITH VARYING DURATION OF OVARIES INSIDE THE UTERUS
Full text linkThis study aims to understand the histopathological changes in the pseudo-pregnant rabbit uterus concerning the duration of ovaries inside the uterus during ovarian transplantation. The study used nine New Zealand White (NZW) pseudo-pregnant female rabbits aged 3-5 years, weighed 1.5-2.9 kg. After a 30-day adaptation period, all rabbits were divided into three treatment groups: K1 (n=3) underwent ovarian transplantation for 3 days, K2 (n=3) for 5 days, and K3 (n=3) for 7 days. Pseudo-pregnant was induced using PMSG and hCG. The rabbits were intramuscularly injected with 100 IU of PMSG, followed by intravenous injection of 75 IU of hCG three days later. Ovary transplantation was performed on the 8th day (day 0 being the day of hCG injection). Uterus was collected after the transplantation procedure according to the respective treatment groups. The results showed that in K1, K2 and K3, there was hyperemia in the endometrial epithelium and infiltration of inflammatory cells. Hyperemia and necrosis were more severe in K1 compared to K2 and K3. In K3, the histopathological appearance of the endometrium tended to be normal, with less damage. In conclusion, the histopathological findings of hyperemia and necrosis in the uterus undergoing ovarian transplantation lasted for 3 days compared to 5 and 7 days
THE EFFECT OF AEROB AND ANAEROBIC CONDITION AND ADDITION OF CaCO3 ON THE GROWTH AND ANTIBACTERIAL ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM LAYER HENS INTESTINE AFTER ADMINISTRATED OF AKBISprob
Full text linkThis study aimed to determine the effect of aerobic and anaerobic incubation, and the addition of CaCO on the growth and antibacterial activity of Lactic Acid Bacteria (LAB) isolated from laying hens intestines (strain ISA Brown) after given 4% AKBISprob. The LAB used in this study was the isolates strains that produced the strongest antibacterial activity with inhibition zone 5 mm. The treatments consisted of two incubations condition (aerobic and anaerobic) with five concentrations of CaCO3 (1%, 2%, 3%, 4%, and 5%). Each treatment consisted of three repetitions. Total Plate Count (TPC) method and the agar diffusion method were used to calculate the amount of LAB and antibacterial activity, respectively. The results showed that anaerobic conditions had a significant effect (P0.05) on the increase of LAB growth, but both of aerobic and anaerobic conditions (P0.05) did not show significant effect on antibacterial activity. The addition of CaCO3 had a significant effect (P0.05) on the increase in growth and antibacterial activity of LAB. Based on the results of the study, it can be concluded that anaerobic incubation can increase the growth of two isolate of LAB isolates (P31S72 and P31H62), however aerobic and anaerobic incubation have no effect on antibacterial activity. In addition, the administration of 3% CaCO3 can increase the growth and antibacterial activity of all LAB isolates
THE EFFECT OF AGE ON WOUND HEALING AND PHYSICAL DEVELOPMENT OF LOCAL CATS (Felis catus) POST-OVARIOHYSTERECTOMY SURGERY
Full text linkThe purpose of this study was to evaluate the effect of age on wound healing and physical development of local cats (Felis catus) after ovariohysterectomy. A total of 15 female cats were divided into three age groups (2-4, 5-9, and 10 months old) with each group consisting of five cats. Thetreatment group was sterilized using the lateral flank ovariohysterectomy method. Observations of surgical procedures and physical conditions were carriedout during anesthesia, including onset, duration of anesthesia and surgery, body temperature, heart rate, and respiratory rate. Post-operative observationswere made to evaluate wound healing, hematological profile (RBC, Hb, PCV, WBC, PLT), body weight, Body Condition Score (BCS), bone density, andgrowth plate closure. The results showed that there was no significant difference (P0.05) during anesthesia, wound healing, or an increase in body weight,BCS, and bone density in all age groups. Ovariohysterectomy at 2-4 months old delayed the closure of the bone growth plates (P0.05).Ovariohysterectomy in pubertal and post-pubertal cats is safe to perform as long as good surgical preparation is provided