Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
Not a member yet
497 research outputs found
Sort by
VENTRAL MEDIAN AND LATERAL FLANK APPROACH FOR OVARIOHYSTERECTOMY IN CAT
Full text linkThis study was conducted to compare Ventral Median Approach (OHM) and Lateral Flank Approach (OHF) for feline ovariohysterectomy (OH). Fifteen healthy local female cats (Felis catus) with body weights ranged 2-4 kg and aged 1-2 years were divided into two groups, OHM (n= 8) and OHF (n= 7). Prior to OH, the cats were anesthethized using ketamine-xylazine and the sugery was performed by the same operator. The wound scoring showed a significantly higher difference in the OHM group on day 3 and 4 post-surgery. Scores of erythematous, swelling and dehiscence showed no significant differences. The 4Avet pain scale was significantly higher in OHF at the 2, 6, and 12-hours post surgery. The pain response was evaluated using baseline tactile Semmes-Weinstein Monofilaments and was significantly higher in OHF than OHM on day 1 to day 5 post surgery. Blood hematology, blood glucose and wound healing duration did not differ significantly between groups. The OHF approach showed greater post-operative pain than the OHM approach. Wound dehiscence was observed in two cats (25%) in OHM group from day 4 post-operative. The OHM approach showed a greater risk of complications than the OHF group and therefore requires better post-operative care. The OHF group showed a smaller risk of complications but requires better post-operative analgesic therapy
HEPARIN AND HYPOTAURINE SUPPLEMENTATION IMPROVE THE FERTILIZATION RATE OF SHEEP OOCYTES MATURED IN MEDIA CONTAINING L-CARNITINE IN VITRO
Full text linkThis study to do examine the effectiveness of using heparin and hypotaurine to improve the in vitro fertilization rate. In first stage, oocytes were matured in the maturation medium supplemented with 0.3 mg/mL L-carnitine to improve nuclear maturation. In second stage, matured oocytes were fertilized with semen incubated in fertilization medium supplemented with 0.1 IU/mL heparin and 10 M hypotaurine. The final concentration of sperm used was 5x106 spermatozoa/mL, and its interaction with oocytes was carried out for 12 hours in 5% CO2 incubator at 38.5 C. The results showed that percentage of oocytes that reached metaphase II was significantly higher (P0.05) in media supplemented with 0.3 mg/mL L-carnitine compared to control (88.24.9% vs 79.85.1%). Furthermore, fertilization rate was characterized by formation of 2 PN was significantly higher (P0.05) in media supplemented with heparin and hypotaurine than control (82.62.0% vs 73.32.4%). In conclusion, L-carnitine supplementation at dose of 0.3 mg/mL on maturation media was effective in increasing percentage of matured oocytes. The combination of 0.1 IU/mL heparin and 10 M hypotaurine in fertilization medium was also successful in elevating the percentage of fertilized oocytes
ROBUST PREDICTION OF MEAT QUALITY ATTRIBUTES USING NEAR INFRARED SPECTROSCOPY
Full text linkThe main purpose of this present study is to evaluate the ability of near infrared technology as an alternative method in determining and assessing quality parameters of meat product where in this case is frozen beef. At first, beef samples from chest and legs parts were sliced and taken at the amount of 100g per sample to be frozen. Then spectra data of beef samples were obtained using near infrared spectrophotometer (PSD IR i16) in wavelength range from 1000 to 2500 nm with optical gain 4x. Actual protein contents were obtained by Kjeldahl method and measured in triplicate. The near infrared spectra data were enhanced and improved by means of mean centering (MC) and baseline shift correction (BSC) methods. The results showed that protein content of frozen beef samples can be predicted rapidly with maximum correlation coefficient is 0.91. Heat properties of beef samples changes exponentially during freezing and thus, optimum freezing temperature and time can be predicted as well. Based on those obtained results, it may conclude that near infrared technology can assess frozen beef qualities rapidly and effectively
SPECTROSCOPIC ANALYSIS OF A COMPOUND ISOLATED FROM THE ETHYL ACETATE FRACTION OF CALOPHYLLUM MACROPHYLLUM SCHEFF AND TOXICITY TESTS USING THE BSLT METHOD AGAINST ARTEMIA SALINA LEACH LARVAE
No full textThe Calophyllum genus has been reported to be rich in bioactive compounds that have medicinal properties. The author will also report the results of the isolation of compounds obtained from the ethyl acetate fraction of the bark of the plant C. macrophyllum Scheff, a type of Calophyllum found in Mount Kerinci, Jambi province. This paper will analyze the spectroscopic data of these compounds which include UV, IR, GC-MS, 1HNMR and 13CNMR spectra which are amplified by HMQC and HMBC spectra. The first step in bioactivity testing is the toxicity test using the BSLT (Brine Shrimp Lethality Test) method using Artemia Salina Leach shrimp larvae. The results of the spectroscopic analysis obtained the chemical formula as a derivative of fatty acid methyl ester (methyl-octadec-14 enoic), in the form of a yellow liquid with a boiling point of 173-175C. The results of the initial screening for bioactivity through the toxicity test of the BSLT method on Artemia salina Leach shrimp larvae obtained an LC50 value of 141.2 g/mL. This compound still has the potential to have certain bioactivit
COMBINATION OF BASIL, TURMERIC AND BEAN SPROUTS TO HEMATOLOGY AND BIOCHEMISTRY OF FEMALE RAT BLOOD BEFORE PREGNANCY
Full text linkThe purpose of the study was to examine the safety of combination of basil, turmeric and bean sprouts extract solution on the hematological parameters and blood biochemistry profile of rats. The combined ingredients used in the study were obtained from different places. A total of 15 rats were divided into 3 groups based on the treatment dose. Each group consisted of 5 rats. The rats in control group (K) did not given extract combination, while the rats in group KKT 1 % and KKT 5% received combination of basil, turmeric and bean sprouts extract at dose of 1% and 5%, respectively. Effectiveness and safety tests were carried out by evaluating the hematological and blood biochemical profiles of female rats. The data were analyzed using one-way analysis of variance (ANOVA). The results showed that the rats given combination of basil, turmeric and bean sprouts extract at a dose of 1% and, dose of 5% did not had significant different compared to control (P0.05), but tend to have positive effects in increasing several blood components that play an important role in maintaining immunity during pregnancy. It can be concluded that the combination of basil, turmeric and bean sprouts at dose of 1% and 5% do not adversely affect hematology profile and blood biochemistry of rats. This indicates that the extract combination does not cause any toxicity effects on the rats
IDENTIFICATION OF REPRODUCTIVE DISORDERS IN FEMALE CATTLE AT LOCAL FARMS IN WOHA SUB-DISTRICT, BIMA REGENCY
Full text linkThis research aims to identify reproductife disorders in female cattle raised by farmers in Woha Sub-district, Bima Regency, West Nusa Tenggara Province. The study involves the identification of 141 female cattle (56 heifers and 85 cows). The research is carried out by bringing the cattle to the designated locations determined by the farmers group leader, which includes fields and barns. Data collection is conducted through rectal examination of the reproductive system and analysis of records from farmers and officials. The research method includes several stages, namely anamnesis, clinical examination, and rectal examination of the reproductive system. The obtained data is then recorded and analyzed descriptively. Based on the examination results, it is known that 210 female cattle experienced reproductive disorders, including silent heat (4.55%), vaginitis (21.8%), follicular cysts (12.73%), persistent corpus luteum (11.82%), ovarian hypofunction (5.45%), and endometritis (20.91%) of cows and silent heat (8%), vaginitis (28%), follicular cysts (12.73 %) corpus luteum persisten (15 %), and ovarian hypofunction (5%) of heifers
DETERMINATION OF OVULATION TIME IN GAYO MARES BASED ON IMAGE OF PREOVULATORY FOLLICLE GROWTH
Full text linkThis study aimed to obtain the ultrasound image of preovulatory follicle growth in gayo mares to predict the ovulation time. This study used three Gayo mares and one gayo stallion for teaser aged 5-10 years and weighed 200-250 kg. Preovulatory follicle growth was observed using transrectal ultrasonography which was performed when the dominant follicle size was 3.5 cm. Observations were made for 3 consecutive estrous periods on gayo mares at relatively the same time. The absence of previous follicular appearance and the presence of hypoechoic area in the same ovary indicate that ovulation has occurred. The result revealed that the diameter of preovulatory follicle of gayo mares was 4.020.32 to 5.050.04 cm. There was no significant follicle growth in the last 48 hours before ovulation and the preovulatory follicle shape altered from spherical to pearshape. Many small follicles (2 cm in diameter) were observed in peripheral area of the ovary indicated the recruitment of follicles which form the first follicular wave. To conclude, the alteration in the size and pearshape of preovulatory follicle and the recruitment of small follicles can be used as a marker to determine the time of ovulation on gayo mares
BOER BUCK SPERM QUALITY IN EXTENDER SUPPLEMETED WITH NON-ENZYMATIC ANTIOXIDANTS AND STORAGE AT COLD TEMPERATURE
No full textThe aim of this study was to determine the durability of standard extender tris egg yolk supplemented with non-enzymatic antioxidants from guava and fig fruit filtrate on the motility and viability of Boer buck sperm. Semen was collected using an artificial vagina every four days for ten replications. The treatment extenders were Tris+2.5% egg yolk (P0) and P0+1.487 mg vitamin A and P0+3.958 mg vitamin C per ml/100 ml (v/v) hereinafter referred as P1 and P2, respectively. The experimental design was a completely randomized 3 times 10 a factorial pattern. The variables assessed were individual motility, progessive motility and viability of sperm evaluated every day for 4 consecutive days. Result indicated that, 4 days after extended and stored at 5C, the average individual motility, progessive motility and sperm viability were 45.27.94; 33.010.2 and 49.67.69 for P1, and 45.46.14; 30.48.38 and 50.55.88 for P2. The data on the three indicators of sperm quality between P1 and P2 did not show a significant difference (P0.05). While for P0 30.65.85; 24.67.30 and 40.75.77, respectively, and were significantly lower (P 0.05) than P0 or P1. It was concluded that semen extended using tris-egg yolk extender supplemented with vitamin A or C could still be used for Artificial Insemination until day 4 after extended and storage at 5C. It is suggested that research needs to be done to produce a ready to use, simple preaparation, cheap and long lasting instant extender
THE GENE EXPRESSION OF ADAM17 AS A GENETIC MARKER OF ALZHEIMER DISEASES IN THE BRAIN OF LONG-TAILED MACAQUES (Macaca fascicularis)
Full text linkThis study aims to identify the expression of the a disintegrin and metalloproteinase 17 (ADAM17) gene as a marker of Alzheimer's disease in long-tailed macaques (Macaca fascicularis). This study used six brain samples (hippocampus and cortex regions) of long-tailed macaques which was divided into two groups consisting of aged long-tailed macaques and adult long-tailed macaques. The expression of ADAM17 gene was determined by comparing the relative quantification values between the two age groups, and brain regions consisting of the hippocampus and cortex regions. The results of data analysis showed no significant difference in the expression of ADAM17 gene between brain regions and between age groups of long-tailed macaques. However, numerically the results showed a lower expression of ADAM17 gene in the hippocampus region of aged macaques. Lower expression of ADAM17 gene could be a marker of old animals indicating the stages of Alzheimer's disease