Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
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ANATOMI DAN GAMBARAN ULTRASOUND ORGAN REPRODUKSI SELAMA SIKLUS ESTRUS PADA KUDA GAYO BETINA (Anatomy and Ultrasound Imaging of Reproductive Organs of Gayo Mares During Estrous Cycle)
Get PDFThe present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Massons trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mares reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mares reproductive tract from labia to apex cornua was 48.001.00 cm. Weight of Gayo mares left ovary was 19.077.70 g and the right was 24.430.83 g. Histologically, there was no difference between Gayo mares structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.430.10 cm in horses with estrous cycle of 21 days and 6.300.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness
STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak))
Get PDFThe objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 g/ml of concentration for PNA lectins and 15g/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis.Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntja
PENINGKATAN AKTIVITAS ENZIM LIPOPROTEIN LIPASE (LPL) DAN PERUBAHAN HISTOPATOLOGIS HATI TIKUS (Rattus norvegicus) HIPERKOLESTEROLEMIA YANG DIBERI EKSTRAK SARANG SEMUT (Myrmecodia sp.) (The Increase Activity of Lipoprotein Lipase (LPL) Enzyme and Histophatological Changes of Liver of Hypercholesterolemic Rat (Rattus norvegicus) Induced by Ethanolic Extract of Ant Plant (Myrmecodia sp.))
Get PDFThis study was aimed to find out the effect of ethanolic extract of ant plant (Myrmecodia sp.) to increase the activity of enzyme lipoprotein lipase (LPL) serum and to observe the histopathological changes of hypercholesterolemic rat liver. This study used 20 male rats grouped into 4 treatment groups, namely negative control group (K1), hypercholesterolemic group (K2), and hypercholesterolemic group that administered with ethanolic extract of ant plant 100 (K3) and 200 mg/kg bw (K4). The LPL enzyme activity were measured by the titration method and histopatological changes of liver were observed by calculated fatty degeneration and fatty infiltration. The data were analyzed using one way anova followed by Duncan test. The average of LPL enzyme activity on group K1, K2, K3, and K4 were 0.800.06, 0.450.10, 0.830.11, and 0.760.03 unit, respectively. The average number of fatty degeneration on hepatocyte and fatty infiltration were 1.800.83, 3.601.14, 23.00 1.22, and 40.201.30; and 9.200.84, 16.401.14, 2.600.54, and 4.800.83, respectively. The results showed that theraphy ethanolic extract of ant plant effects significantly (P0.01) on the increase of enzyme LPL and improve liver damage in hypercholesterolemic male rats. To conclude the administration of ethanolic extract of ant plant increases the LPL enzyme activity and improves liver damage on hyperch olesterolemic rats.Key words: Myrmecodia sp., LPL activity, histopathological liver, hypercholesterolemi
EFEKTIVITAS FERMENTASI SUSU KAMBING DENGAN PENAMBAHAN Lactobacillus rhamnosus SEBAGAI INHIBITOR TIROSINASE (Fermentation Effectivity of Goat Milk added Lactobacillus rhamnosus as Tyrosinase Inhibitor)
Get PDFThis study aimed to investigate the effectivity of goat milk fermentation as tyrosinase inhibitory with Lactobacillus rhamnosus TW 2. The examination of fresh milk contained the density, protein, and fat content. The culture of Lactobacillus rhamnosus TW 2 acid lactid bacteria starter was added with the concentration of 3, 4, and 5%, inoculated in pasteurized goat milk, then incubated at 37 C for 24 hours. Fermented milk were extracted by centrifugation, then supernatant was collected and was used for inhibition of tyrosinase enzymes activity on L-tyrosin and L-dopa substrate. The result showed that the density, fat content, and protein content of Etawah crossbred goat milk are 1.028, 3.73, and 5.45%, respectively. Re-identification of lactic acid bacteria showed similar morphology, physiology, and bio-chemistry with the isolated lactic acid bacteria. The growth curve of TW 2 was observed in 12 hours. The 5 % of Lactobacillus rhamnosus TW 2 was the best concentration to inhibit tyrosinase activity in L-Tyrosin substrate. In conclusion, fermentation of goat milk using Lactobacillus rhamnosus TW 2 at concentration of 5% as starter is effective to inhibit tyrosinase activity significantly.Key words: goat milk, fermentation, tyrosinase inhibitor
DETEKSI GEN PENYANDI ADHESIN PADA VEROCYTOTOXIGENIC Escherichia coli (VTEC) ISOLAT SAPI (Detection of Gene Encoding Adhesin of Verocytotoxigenic Escherichia coli (VTEC) Isolated from Cattles)
Get PDFThis study was aimed to perform phenotypic and genothypic characterization of Escherichia coli (E. coli), particularly VTEC strain isolated from cattle faeces. In this study, 25 E.coli isolated from faeces specimens and faeces base fertilizer of dairy and beef cattles were used. Examination were carried out using phenotypic and genothypic characterization which is specified for E coli VTEC strain. The result showed that 20 % samples of fresh faeces specimens were detected as VTEC strains and none of isolate was detected from faeces base fertilizer samples. From VTEC strains, could detect 16 % VT1 gene, 12 % VT2 genes and 8% of both. Detection on gene pyelonephritis-associated pilli (pap), S fimbrial adhesion (sfa), and afimbrial adhesion (afa) were found about 60%, 80% and 80%, respectively
PEMBERIAN PASAK BUMI MEMENGARUHI KADAR TESTOSTERON DAN SPERMATOZOA KAMBING PERANAKAN ETAWA (The Administration of Eurycoma longifolia Jack Affect the Level of Testosterone and Spermatozoa of Etawa Crossbreed Goat)
Get PDFABSTRACT The study aimed to find out the effect of Eurycoma longifolia Jack on testosterone level and spermatozoa quality of Etawa crossbreed goat. Goats were divided into two treatment groups, three goats each. Group 1 (K1) was control group which given orally 20 ml of distilled water every morning at 9:00 am for six days, and group 2 (K2) was administered with Eurycoma longifolia with the dose of 90 mg/kg bw in 20 ml of distilled water orally every morning at 9:00 am for six days. The concentration of testosterone was measured on day 1, 3, and 6 using the method of enzyme-linked immunosorbent assay (ELISA). The quality of sperm consisted of concentration and percentage of life sperm. The results showed that level of testosterone on K2 increased started from day 1 (9.36 ng/ml) to day 6 (12.43 ng/ml) (P0.05). The percentage of life spermatozoa increased higher on K2 compare to K1 on day 3 to day 6, that was 88.6 and 89.8% on K1 to 91.0 and 92.7% on K2. In conclusion, the administration of pasak bumi with the dose of 90 mg/kg bw in 20 ml of distilled water for 6 days able to increase testosterone levels and percentage of life sperm of Etawa crossbreed goat
MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)
Get PDFThis study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (2 years), six kids (6 months), and six does (2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.654.27%, 10%, 15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goa
PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram)
Get PDFABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets were diluted with Tris extender (control), Tris extender + 0.4% dextrose (dextrose), Tris extender + 0.4% maltose (maltose), Tris extender + 0.4% lactose (lactose), and Tris extender + 0.4% sucrose (sucrose), respectively. There were no significantly difference among treatment on mean percentages of motile (MS), live (LS) and intact plasma membrane (IPM) after equilibration. Mean percentages of post-thawed MS, LS, and IPM for dextrose (43.0, 53.8, and 53.4%), maltose (44.0, 54.8, and 52.8%), lactose (44.0, 52.8, and 52.8%), and sucrose (44.0, 55.0, and 53.4%) were significantly (P0.05) higher than control (37.0, 44.0, and 40.8%). In conclusion, addition of 0.4% dextrose or 0.4% maltose or 0.4% lactose or 0.4% sucrose in Tris extender increased quality of frozen thawed cauda epididymal spermatozoa of ram
PENGARUH PEMBERIAN EKSTRAK BUAH MENGKUDU (Morinda citrifolia) DALAM LARUTAN NATRIUM KLORIDA FISIOLOGIS SEBAGAI BAHAN PENGENCER SEMEN TERHADAP PENINGKATAN KUALITAS SPERMATOZOA AYAM BURAS PADA SUHU RUANG (Effect of Noni Extract Fruit (Morinda citrifolia) in Physiological Saline Solution as Diluent on the Native Chicken Spermatozoa Quality at Room Temperature)
Get PDFThe purpose of this research was to analyse the quality and storability of native chicken semen at room temperature after diluted with physiological saline solution (NaCl) supplemented with noni fruit extract. Research methodology used was nested randomized design which variables observed covering the quality of chicken spermatozoa macroscopically and microscopically with the doses of noni fruit extract of 0 % (P0), 10 % (P1), 20 % (P2), and 30 % (P3). Rate of spermatozoa motility at 0, 1, 2, and 3 hours on P0 were 83.704.74, 63.309.63, 32.908.52, and 15.006.20; P1 were 85.305.44, 72.7010.06, 51.9011.75, and 28.505.68; P2 were 84.606.40, 78.508.59, 57.9010.73, and 33.709.06; and P3 were 81.807.30, 64.207.93, 40.3011.66, and 19.807.47, respectively. Rate of spermatozoa viability at 0, 1, 2, and 3 hours on P0 were 85.309.09, 65.106.15, 32.1011.86, and 10.309.09; P1 wer 87.406.22, 72.705.33, 50.8013.59, and 26.5010.99; P2 were 68.585.30, 77.704.79, 56.3013.76, and 32.7013.79; P3 were 81.208.04, 68.7010.40, 36.2016.61, and 16.9011.93, respectively. Rate of spermatozoa abnormality at 0, 1, 2, and 3 hours on P0 were 8.703.40, 12.204.42, 17.805.67, and 20.306.38; P1 were 7.802.04, 9.802.69, 13.604.45, and 16.505.19; P2 wer 8.404.33, 10.002.45, 12.505.21, and 15.803.71; and P3 were 9.603.41, 10.902.64, 17.105.61, and 21.208.16, respectively. It can be concluded that the addition of noni fruit extract at the dose of 10 % and 20 % in physiological saline solutin maintain the native chicken spermatozoa quality up to 2 hours post storage at room temperature
KONFIRMASI AVIAN PARAMYXOVIRUS TIPE 1 (APMV-1) SECARA HISTOPATOLOGIS, SEROLOGIS, DAN MOLEKULER (Confirmation of Avian Paramyxovirus Tipe 1 (APMV-1) Infection by Histopathology, Serology, and Molecular Method)
Get PDFResearch was conducted to detect APMV-1 infection by examining microscopic lesions of chicken suspected ND and confirming the causative agent with serological and molecular assay. Samples obtained from commercial and back yard farm in 9 regencies and city of Bali Province were tested by rapid test for AIV antigen detection. AI negative samples were necropsied, then brain, lungs, and intestines were collected for histopathological examination. Samples tissue of brain, lung, spleen, and intestine were taken aseptically for viral isolation and amplification. Infected allantoic fluid was collected and tested by hemagglutination assay (HA) and hemagglutination inhibition (HI) test to prove APMV-1 serologically. Viral ribonucleic acid was isolated and subsequently reverse transcribed by reverse trasncription reaction followed by amplification by polymerase chain reaction to multiply the cDNA. Microscopically, perivascular cuffing (20%), endoteliosis (75%), and gliosis (75%) were found in the brain. In the lung, an interstitialis pneumonia (50%), lobar pneumonia (5%), and proliferation of pneumosit type 2 (100%) were observed. The most prominent intestinal lesions were catarrhal enteritis (75%) and hemorrhagic necrotizing enteritis (10%). Confirmation of the 20 isolates obtained in this study both serologically and molecularly were positive APMV-1. Moreover PCR results showed that 80% of its amplicon showed a single band and 20% still require some optimizations to get single good bands