Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
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ULTRASOUND IMAGING OF POSTPARTUM UTERINE INVOLUTION AND OVARIUM DYNAMIC IN ONGOLE CROSSBREED COWS
Get PDFThis study aimed to determine ultrasound of uterine involution and postpartum ovarian dynamics on Ongole Crossbreed Heifer (PO) associated with postpartum estrus signs. This study used 6 PO cows which were divided into primiparous group and pluripara group. The observation of uterine involution and ovarian dynamics was started from the first day postpartum using ultrasonography (USG) with a linear probe rectally, while the reproductive organs images were recorded every 2 days. The results showed that the time needed for all reproductive organs of 3 primiparous groups to complete the uterine involution after parturition was 37.331.15 days when the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine were 3.140.00 cm, 2.860.00 cm, 3.200.06 cm, 4.660.01 cm, and 4.660.01 cm, respectively. The time needed for all reproductive organs of 3 pluripara groups to complete uterine involution postpartum was 38.671.15 days with the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine 3.180.00 cm, 2.700.02 cm, 3.080.02 cm, 4.420.01 cm, and 4.420.01 cm, respectively. The average times of the first and second ovulation of primiparous cattle were 27.671.15 and 47.671.15 days postpartum, whereas in pluripara group was 28.331.15 and 48.331.15 days postpartum. At first ovulation all cows were not accompanied by signs of estrus, while at the second ovulation 2 primiparous cows and 1 pluripara cow showed less obvious signs of estrus, 1 primiparous cow and 1 pluripara cow showed signs of medium estrus, and 1 pluripara cow showed clear estrus signs
PHYLOGENETIC ANALYSIS AND VIRULENCE-CODING GENES CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM LAYING HEN BRAIN WITH TETELO SYMPTOM
Get PDFNewcastle disease (ND) is an acute poultry disease caused by Paramyxovirus group. It has characteristic neurological symptoms, called torticollis. The molecular assay to find out the presence of viral genes in the brain can be an option in detecting ND virus infections since it penetrates brain barrier system. The purpose of this study was to identify ND viruses in the brain of chickens with torticollis symptoms, to analyze its phylogenetic and to characterize its virulence genetic code. Samples used were 12 dead chickens with historically had torticollis symptoms, obtained from poultry farms at several areas in West Java and Banten. Chicken brains were prepared for reverse transcriptasepolymerase chain reaction (RT-PCR) test. All positive samples then sequenced to obtain its nucleotide sequences from some of Fusion (F) genes analyzed its phylogenetic by comparing with Indonesian ND isolate virus from GenBank using Mega X software. The results of RT-PCR test showed that only one sample (Virus MSL.03) contained genes of ND virus. Based on homology tests and phylogenetic analysis, the virus belonged to subgenotype VIIh with an identical level of 95.34-95.86% when compared to several isolates from Indonesia. The MSL.03 ND virus has 112RRRKRF117 pattern in F0 indicatest its virulent category
CORRELATION BETWEEN BLOOD METABOLITE AND REPRODUCTIVE PERFORMANCE OF LACTATING HOLSTEIN FRIESIAN CROSSBRED COWS IN SMALLHOLDER FARMERS
Get PDFThis study examined the correlation between the concentration of blood metabolites (triglyceride, albumin, phosphorous) on the reproductive performances (S/C and PPM) of lactating Holstein Friesian crossbred (HFC) cows. Blood samples were collected through the caudal vein 8 hours after feeding and the plasma was analyzed at the Integrated Research and Testing Laboratory of Universitas Gadjah Mada (LPPT UGM). Samples were stored at 5 C. Triglyceride, albumin, and phosphorous were measured using glucose oxidase-phenol 4-aminoantipyrine (GOD-PAP) method, photometric bromocresol green method, and cypress diagnostic, respectively. Blood metabolites data were analyzed using Pearson correlation model. The results showed that the correlation coefficients of triglycerides, albumin, and phosphorus levels on PPM and S/C were -0.521 and -0.650; -0.447 and -0.612; -0.513 and -0.700, respectively. In conclusion, there are significant negative correlation between triglyceride, albumin and phosphorus levels on the PPM, and very significant negative correlation between triglyceride, albumin and phosphorus levels on the S/C of lactating HFC cows
THE ADDITION OF FRESH GARLIC (Allium sativum L) IN DUCK FEED REDUCES CHOLESTEROL CONTENT OF DUCK EGG
Get PDFThe objective of this study to determine the effect of feeding fresh garlic to ducks on decrease the cholesterol level of duck eggs. This study composed of two treatment groups. The duck in the first group (control) was fed local feed without spplemente d with fresh garlic, while in the second group was fed with local feed and supplemented with 50 grams of fresh garlic/duck/day. Egg yolk cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TG) analysis were carried out at the end of the study using the ether extract method and then with the Cholesterol Oksidase Para Amino Penazone (CHOD-PAP) or enzymatic color test. Data was analysed using one way Analysis of Variance (ANOVA) the results showed that fresh garlic reduced total cholesterol, LDL fraction, and triglyceride, but increased the HDL fraction of duck egg yolk. The total cholesterol, LDL fraction, and triglyceride of duck egg yolks dropped by around 15.98 mg per dL (8.06%), 10.40 mg per dL (12.97%), 64.92 mg per dL (12.71%), respectively, while the HDL fraction increased by 11.40 mg per dL (19.32%) The results of this study indicated that fresh garlic can be used to produce specific products such as low cholesterol and low fat duck eggs
THE COMPOSITION OF SATURATED AND UNSATURATED FATTY ACIDS AND CHOLESTEROL IN MEAT OF BALI BULL FATTENED USING GREENLOT SYSTEM IN SMALL HOLDER FARMING
Get PDFThe objective of this research was to analyze the composition of saturated fatty acid, unsaturated fatty acids, and cholesterol in meat of Bali bull fattened using forage crops in small holder farming. The experiment was conducted for 3 months using nine Bali bull aged 2.5-3.5 years old with the initial body weight of 227-290 kg in the feedlots of Bero Sembada Farmers Group, Laen Manen Sub District, Malaka Regency, East Nusa Tenggara. The experiment was conducted based on the fattening system of local farmers in terms of feeding, housing, and health management. Feed given during the experiment was Centrosema pubences, Clitoria ternatea, fresh corn straw, king grass, Leucaena leucocepala, natural grass, Pennisteum purpureum, and Sesbania grandiflora. At the end of this study, cattle were slaughtered and the composition of saturated fatty acid, unsaturated fatty acids and cholesterol were measured. Data were analyzed using a descriptive statistic. Results revealed that the total of saturated fatty acid and unsaturated fatty acids in meat were 36.628% and 26.739%, respectively, while the cholesterol reached 79.18 mg/100 g of meat. In conclusion, meat of Bali bull fattened using forage crops in small holder farming has higher saturated fatty acid than unsaturated fatty acids
IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM
Get PDFThe aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5 C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming