Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
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PREVALENCE OF MARES GRANULOSA THECA CELL TUMOR (GTCT) IN INDONESIA
Get PDFThis study was conducted to determine the prevalence rate of mares GTCT in Indonesia. Ultrasound examination of ovaries were performed on mares in Java and Madura Island of Indonesia. Thirteen mares had been affected by GTCT from 2913 number of examinations. The prevalence rate of GTCT during year 2006 and 2007 were 4.71% and 5.56%, respectively followed by a decrease in prevalence rate of GTCT during 2008-2014. The reproductive cycle in a mare with GTCT had returned to normal 6 months after ovariectomy
FEEDING LOCAL FERMENTED WITH EM-4, AMMONIUM SULFATE, AND UREA ON THE BLOOD FAT LEVEL OF MALE DUCK
Get PDFThe objective of this study was to determine the effect of commercial ration by the addition of ammonium sulfate and urea fermented feed ingredients on the blood fat levels of local male ducks. This study used a completely randomized design with 32 male ducks which weredivided into four groups namely R0, R1, R2, R3, and R4. Each group was given the following treatment: R0 only given commercial ration;R1 was given 80% commercial ration + 20% fermentation of EM-4 + 1.5% urea); R2 was given 80% commercial ration + 20% EM-4 + 1.5%ammonium sulfate fermentation; and R3 was given 80% commercial ration + 20% EM-4 + 1.5% urea + 1.5% ammonium sulfate. Variablesin this study were blood fat consisting of cholesterol, triglyceride, High-Density Lipoprotein (HDL), and Low-Density Lipoproteins (LDL).The data obtained were analyzed by using 5% real level and LSD. The results showed that the provision of commercial ration added withlocal feed fermented with ammonium sulfate and urea had a significant effect on cholesterol levels (P0.5), but not significant (P0.05) ontriglyceride, HDL, and LDL levels. Furthermore, the ration that gave the best effect on the blood fat levels of male ducks blood was the ration given to R3 group
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO
Get PDFThis study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis.A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL.The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies.Positive results showed a brownish color on the oocyte and its cumulus.The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased.It can be concluded that the addition of urea does not affect the level of apoptosis
THE EFFECT OF COGON GRASS ROOT ETHANOL EXTRACT ON REPRODUCTIVE ORGAN AND EPIDIDYMAL SPERM QUALITY OF MOUSE WITH DIFFERENT AGES
Get PDFThis study aims to determine changes related to epididymal sperm parameters in mice given cogon grass root ethanol extract (CGREE). The experiment was carried out by dividing three groups of mice aged 4-week old (early pubertal), 8-week old (adult), and 24-week old (old) as control and treatment groups. The treatment groups were given 115 mg/kg body weight of CGREE for 14 days. The mice were sacrificed on day 15 to asses reproductive organ indexes and quality of the epididymal sperm. Index of epididymis and ductus deferens indices significantly reduced in the 4-week old treatment group compared to control (0.23 vs 0.33; 0.08 vs 0.16, respectively), and vesical seminal significantly reduced in 8- week old treatment group compared to control (0.76 vs 0.68). The epididymal sperm quality significantly decreased in all treatment groups, but the sperm concentration in early pubertal group showed an increase. These results indicate that CGREE can enhance several semen parameters in prepubertal and adult mice but this effect is not apparent in old mice
CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE
Get PDFThe objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter () of follicles: small follicles ( 1 - 5 mm), medium follicles ( 5 - 8 mm), and large follicles ( 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations
EGG QUALITY OF SELECTED ACEH NATIVE CHICKENS FED ON THE DIETS COMPOSED OF Indigofera sp. MEAL BASED ON FEED FERMENTATION AS PARTLY SUBSTITUTION OF COMMERCIAL DIET
Get PDFThe objective of this study was to determine the effect of inclusion of fermented feed consisted of 15% Indigofera sp. + 40% rice bran + 25% corn bran + 20% soybean meal as partial substitution of commercial diet on the egg quality of selected Aceh native (SAN) chickens. As many as 48 SAN pullets were used in this study. The study was performed in a completely randomized design, consisted of 4 treatments and 4 replications. Each replication was an experimental unit consisted of three chickens. The results of study indicated that inclusion of up to 15% fermented feed which was composed of 15% Indigofera sp. + 40% rice bran + 25% corn bran + 20% soybean meal as substitution of commercial layer diet significantly (P0.01) improved yolk index without adverse effect on albumen and eggshell quality of SAN chickens. As conclution, substitution of fermented feed contained 15% indigofera may improve the egg quality of SAN chickens
THE IMPROVEMENT OF SEMEN QUALITY AND TESTOSTERONE LEVEL OF BALI CATTLE AFTER PROSTAGLANDIN F2 ADMINISTRATION
Get PDFThis study aimed to determine the effect of prostaglandin F2 (PGF2) administration on improving semen quality and testosterone levels on Bali cattle. This study used 3 Bali cattle aged 2, 3.5, and 5 years old. The sample used in this study was Bali cattle semen. In this study, the Latin Square Design was used with three different treatments were administered over three time periods. The treatments performed were P1 (5 mL physiological NaCl), P2 (25 mg prostaglandin), and P3 (37.5 mg prostaglandin) while treatment period was three weeks of treatment administration, which were 1st. 2nd, and 3rd week. Blood collected through coccygea vein 30 minutes after treatment to measure testosterone levels using enzyme-linked immunosorbent assay (ELISA) technique. Statistical analysis showed that PGF2 administration at a dose of 25 mg and PGF2 at a dose of 37.5 mg had no effect (P0.05) on volume, color, pH, consistency, concentration, and motility of Bali cattle spermatozoa. The volume of semen obtained from P1; P2; and P3 were 6.50.9; 6.30.6; and 6.21.2 mL, respectively. The color of the semen in P1, P2, and P3 were beige and milky white. The pH in groups P1; P2; and P3 were 6.70.6; 7.10.2; and 6.50.2, respectively. Spermatozoa concentration in P1; P2; and P3 were 1,32896.43 x 106 cells/mL; 1,354102.19 x 106 cells/mL; and 1,353.388.55 x 106 cells/mL, respectively. Spermatozoa motility in P1; P2; and P3 were 73.32.8%; 71.62.8%; and 73.32.8%, respectively. Testosterone levels in P1 and P3 were 5.050.22 and 6.741.38 ng/mL, respectively (P0.05). Based on the results of this study, it was concluded that the administration of PGF2 does not affect semen quality but can increase the level of testosterone on Bali cattle
PREVALENCE AND RISK FACTOR OF STONGYLOIDOSIS IN BEEF CATTLE IN KASIMAN SUBDISTRICT, BOJONEGORO
Get PDFA pair of cross-sectional studies was conducted to determine prevalence and risk factors of Strongyloides spp. (threadworm) infections in beef cattle in Kasiman Subdistrict, the Regency of Bojonegoro. A total of 263 and 270 of local beef cattle with various age were selected for faecal sample during dry season (August-October) and wet season (February-March), respectively. Feces were processed for counting the number of eggs per gram feces (EPG) using McMaster method. The prevalence of strongyloidosis was 4.56% and 7.04% in the dry season and the rainy season, respectively. The factors that associated with prevalence and intensity infection were age and sex of cattle. During the dry season and the rainy season, the calf and weaner group (1 year) was more susceptible for Strongyloides infection than cattle with older age and showed higher intensity of infection (based on EPG values). The bulls showed a higher risk and intensity of infection than cows
