Jurnal Penelitian Tanaman Industri
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Pestalotiopsis desseminata PADA JAMBU MENTE BIOLOGI DAN INTERAKSINYA DENGAN Helopeltis antonii
No full textPestalotiopsis desseminata on cashew: its biology and interaction with Helopeltis antoniiPestalotiopsis desseminata is one of pathogens causing leaf spots. die-back of shoots and inflorescence of cashew plant. It is assumed there is an interaction between the attack of Helopeltis antonii and the fungus in Ihe ield so that the damage becomes more devastating. The objectives of (his research were to investigate several aspects of fungal biology (pathogenicity, mycelial growth, acervuli production, sensitivity to fungicides) and its interaction with //. antonii. The esearch was carried out from April 1999 to March 2000 at the laboratory and green house of Pest and Disease Department Research Institute for Spice and Medicinal Crops, Bogor. /' desseminata was diectly isolated from cashew leaves. Pathogenicity test of P. desseminata isolate was conducted on cashew seedlings. The fungal growth and fungicide effects were carried out in vitro on agar medium. The interaction between P. desseminata and H. antonii was examined by inoculating the fungus and insect, either individually or in combination, on cashew seedlings. The results showed that P. desseminata isolate fomis black spherical acervuli containing oblongs conidia with 5 cells, and the outermost cell has 3 flagellate. Acervuli could only be produced on the cultue illuminated continuously by 600 lux translucent lamp. Pathogenicity test of several isolates of /' desseminata on cashew seedlings caused spherical leaf spot symptom, individually or coalesced as bigger leaf spots. In vitro fungal mycelia growth could be suppressed by several fungicides, including bcnomyl 50% (I ppm) and Uiiopanate-mcthyl 70% (10 ppm) Combining inoculation between P. desseminata and //. antonii resulted in moe severe (devastating) die-back compared with individually inoculated
PENELITIAN POLA USAHA TANI BERBASIS KELAPA HIBRIDA DI CIMERAK
No full textPenelitian dilaksanakan dari Januari 2000 s/d Desember 2001 pada kebun kelapa hibrida rakyat pasca konvcrsi, pescta Proyck Perkebunan Inti Rakyat PTP XIII (sekarang PTP Nusantara VIII) di Desa Sindang Sari, Kecamatan Cimerak, Kabupaten Ciamis, Propinsi Jawa Barat. Bahan penelitian adalah hamparan kelapa hibrida PB 121 monokultur dan produktif dengan tahun tanam 1984/1985 seluas 5 ha. Jarak tanam 9 m x 9 m dengan sistem tanam segitiga sama sisi. Areal petanaman berada pada kctinggian 47 m diatas permukaan laut dengan curah hujan rata-rata 3 450 mm/tahun dan jenis tanah podsolik merah kuning dengan pH 6.0. Kegiatan studi dilakukan melalui dua tahapan. Petama dilakukan studi PRA untuk melakukan idcntifikasi daerah penelitian, keadaan sosial ekonomi petani dan tanaman sela yang prospektif unluk dijadikan sebagai tanaman sela. Kedua dari hasil studi PRA ditctapkan lima pola usaha tani berbasis kelapa untuk diuji dalam penelitian ini yaitu (1) pola usaha tani kelapa (monokultur, tradisional), (2) pola usaha tani kelapa + kelapa disadap (pola rakyat, tradisional), (3) pola usaha lani kelapa + disadap 30 pohon/ha - tanaman sela pisang satu baris, (4) pola usaha lani kelapa + disadap 30 pohon/ha - tanaman sela pisang dua baris dan (5) pola usaha tani kelapa + disadap 60 pohon/ha + tanaman sela pisang satu baris Masing- masing pola seluas satu hektar. Hasil penelitian menunjukkan bahwa pola usaha tani kelapa (monokultur) dan pola usaha tani kelapa + disadap (pola pctani) tidak layak unluk diusahakan karena secara ckonomis tidak mengunlungkan. Hasil analisis kelayakan usaha tani diperoleh bahwa dari lima pola usaha tani berbasis kelapa yang diuji menunjukkan bahwa dua pola usaha lani yang mengunlungkan dan layak unluk diusahakan yailu petama pola usaha tani kelapa + disadap 30 pohon kelapa per hektar - tanaman sela pisang satu bais dengan nilai B/C ratio 1.23, NPV sebesar Rp. 3 319 690 dan IRR 42.24%. Kedua adalah pola usaha tani kelapa + disadap 60 pohon kelapa per hektar + lanaman sela pisang satu bais dengan nilai B/C ratio 1.44, NPV sebesar Rp. 9 096 815 dan IRR 69.36%. Kedua pola usaha tani berbasis kelapa ini, dapal dianjurkan untuk dikembangkan sebagai upaya meningkatkan pendapatan dan kemampuan petani pasca konvcrsi untuk membayar kembali kidit investasi yang telah dikeluarkan pemerintah.Kata kunci: Kelapa hibrida, usaha tani, Ciamis, Jawa Barat ABSTRACTHybrid coconut basedfarming systems in CimerakThis research was conducted from January 2000 to December 2001 at the farmer's hybid coconut plantation Sindang Sari Villages, Cimerak, Ciamis District, West Java Province. The farmers used to be the members of nucleus estate project PTP XIII (now PTP Nusantara VIII). The object of die research was monoculture coconut hybrid PB-121 plantation of 5 ha planted in 1984/1985. The plant spacing was 9 m x 9 m triangle. The elevation was 47 m above sea level, the average rainfalls was 3 450 mm/year, the soil was yellow red podzolic, and the pH was 6.0. The research activities were carried out in two phases. Firstly, PRA study was conducted to identify die research location, socioeconomic condition, and prospective intercrops. Secondly, from the result of PRA study ive fanning systems were selected for the study, i.e. (I y traditional monoculture coconut farming system, (2) traditional coconut farming - tapped coconut, (3) coconut farming ♦ lapped coconut 30 palms/ha < one row of banana plants, (4) coconut farming + tapped coconut 30 palms/ha + two rows of banana plants, and (5) coconut farming + tapped coconut 60 palms, ha + one row of banana plants. Each farming systems was one hectare. The results of the research showed that the first and the second farming systems were not good because Uiey were not economically proitable. The farming in systems that were good and proitable were the third and the ifth farming systems, i.e. coconut faming + tapped coconut 30 palms/ha + one row of coconut plants with B/C ratio 1.23, NPV Rp.3 319 690, IRR 42.24%, and coconut farming tapped coconut 60 palms/ha + one row of coconut plants wiUi B/C ratio 1.44, NPV Rp. 9 096 815, and IRR 69.36%. Those two farming systems can be recommended to increase the income of Ihe coconut farmers ater the conversion, so that Ihcy arc able lo pay the credit thai were given by the govenment.Key words ; Coconut, farming system, intercrops, Ciamis District, West Jav
PERBAIKAN REKOMENDASI PAKET PHT UNTUK PENGENDALIAN HAMA Helicoverpa armigera Hbn. PADA TANAMAN KAPAS
No full textRekomendasi pengendalian hama terpadu (PUT) hama utama lanaman kapas, akan tetapi secara tcknis dan ekonomis masih sulit diterapkan oleh petani. Unluk memperbaiki rekomendasi itu maka dikaji efisiensi pemanfaatan parasitoid Trichogrammatoidea armigera N&N. HeUcoverpa armigera nuclear polyhedrosis virus (//oNPV). dan insektisida nabati serbuk biji mimba (SBM) terhadap // armigera. Penelitian dilaksanakan pada musim lanam 199899 di kebun Instalasi Penelitian Tanaman Tembakau dan Serat, Asembagus, Situbondo, Jawa Timur. Rancangan penelitian adalah acak kelompok dengan 6 perlakuan, dan 3 ulangan Ukuran plot adalah 50 m x 50 m Pada rekomendasi standar rakitan komponen PITT, perlakuan yang ditambahkan adalah (1) penyemprotan SBM. (2) pelepasan T. armigera ■ penyemprotan SBM, (3) pelepasan '/'. armigera ' penyemprotan insektisida kimia, (4) penyemprotan faNPV I penyemprotan insektisida kimia. (5) penyemprotan insektisida kimia, dan (6) lanpa perlakuan (kontrol). Penyemprotan SUM, //oNPV, dan insektisida kimia dilakukan apabila populasi ulat // armigera mencapai ambang pengendalian (4 tanaman lerinfestasi per 25 tanaman). dan pelepasan 7 armigera dilakukan apabila populasi telur //. armigera mencapai ambang pelepasan (25 telur per 25 tanaman) Penyemprotan SBM, pelepasan T. armigera dan penyemprotan //oNPV dapat ditambahkan pada rekomendasi standar rakitan komponen PHT. Penambahan penyemprotan SBM pada rekomendasi standar rakitan komponen PITT adalah paling cisien diantara tiga komponen PITT yang ditambahkan, memberikan eisiensi (Marginal Regional Rate of Return. MRR) scbasar 3.64 dengan produktivitas kapas berbiji 1 562 kgha (meningkatkan 392 kg atau 25%) dan biaya penyemprotan Rp 172 267 (menurun Rp 259 883 atau 60%).Kata Kunci : Azadirachla tndica A. Juss, Trichogrammatoidea armigera N&N, WoNPV, HeUcoverpa armigera Hbn . PITT, Gossypium hirsutum I. ABSTRACT Improved 1PM recomendation package for controlling Helicoverpa armigera Hbn. on cotonAn experimcnl on Ihe use of IPM components for controlling HeUcoverpa armigera Hbn. as ihe pests of cotton was conducted in Asembagus Research Station during cotton planting season 1998/99 Ihe objective of this experiment was to assess the usefulness of Trichogrammatoidea armigera N&N., //oNPV, and botanical insecticide (neem seed powder) in improving the IPM recommendation package, so that it can be adopted by farmers This experiment was arranged in randomized blok design with 6 treatments and 3 replicates. Ihe size of each plot was 50 m X 50 m Ihe treatments comprise: (1) neem spray; (2) T. armigera release neem spray; (3) T. armigera release i insecticide spray; (4) //(A'I'V spray I chemical. (5) chemical; and (6) control (unlrcatment) were added lo the standar IPM recommendation package. Neem, /YoNPV, and chemical spray was done whenever population of H. armigera larvae reached action threshold (4 infected plants 25 plants) and release of T. armigera was done whenever population of H. armigera eggs reached "release threshold" (25 cggs/25 plants) Neem seed powder spray, T. armigera release, and //oNPV spray could be added to (he standar IPM recomendation package. Ihe most efficient among this three components was neem seed powder spray. Which gives eicjency Marginal Rate of Return (MRR) 3 64, cotton seed productivity I 562 kgha (increase 392 kg or 25%) and spraying cost Rp 172 267 (decrease Rp 259 883 or 60%).Key words: Azadirachla indica A. Juss., Trichogrammatoidea armigera N&N, 7/aNPV, Helicoverpa armigera Hbn , IPM, Gossypium hirsutum I
REKAYASA OVEN PORTABEL-HORISONTAL PADA PENGOLAHAN TEMBAKAU VIRGINIA
No full textOven portabel-horisontal (4m x 8m x 4m) untuk pengovenan daun tembakau Virginia menjadi krosok fc (flue-cured) telah direkayasa di Balai Penelitian Tanaman Tembakau dan Serat, Malang, Indonesia. Konstruksi oven terdiri alas komponen dinding oven berisolasi (80cm x 200cm), kerangka dari besi siku dan bcsi U, kolektor surya datar (solar flat- collector) yang dipasang pada atap oven. Sistem pcmanas kompor Bros dengan pemanasan tidak langsung. Pengujian dilakukan pada bulan Agustus sampai dengan Oktober 2002 di Lombok Timur, Nusa Tenggara Barat. Model oven portabel-horisontal ini diharapkan dapat menghasilkan suhu niang oven yang seragam sehingga dapat digunakan untuk mengoven daun tembakau yang persentase masak optimalnya tinggi (90- 95%), hemat bahan bakar, dapat dipasang mendekati areal tanaman sehingga hemat ongkos angkut dan dapat menekan kerusakan pasca panen. Hasil penelitian menunjukkan rata-rata selisih suhu ruang atas dan ruang bawah, serta ruang sebclah kanan dan sebelah kiri, masing-masing 2.53°C dan 2.30°C sedangkan selisih kelembaban udara pada posisi yang sama masing-masing hanya 4.55% dan 3.64%. Kadar gula krosok yang dihasilkan berkisar antara 17.19-19.47% dan nikotin 3.16-3.73%. Konsumsi minyak tanah 1.09 l/kg krosok atau 1.16 l/kg krosok jika tanpa kolektor surya dan hanya 40.92% dibanding oven biasa yang memeriukan 2.39-2.80 l/kg krosok. Kolektor surya datar menyumbang 952 625 kJ setara 28.24 I minyak tanah atau 5.80% dari konsumsi minyak tanah, meskipun alat ini memeriukan 17.16% dari total investasi oven. Panas yang hilang melalui dinding karena konduksi hanya mencapai 3.57%, lebih rendah dibanding kehilangan panas pada dinding bata yang mencapai 12.7% - 16.0%. Hasil grading krosok menghasilkan harga jual rata-rata Rp. 12.275/kg kosok, sedangkan hasil analisis ekonomi menunjukkan rasio BC = 1.29; NPV = Rp. 41 962 590 dan IRR = 51.83% atau masih membei peluang keuntungan dan pengembalian kredit. Hasil perhitungan simulasi jika digunakan oven horisontal sederhana dengan harga Rp. 8 000 000 tiap unit, harga krosok Rp. 10 000, Rp. 12 500, dan Rp. 15 000 tiap kg dan harga minyak tanah Rp. 1 000, Rp. I 250 dan Rp. I 500 tiap liter masih memberi indikasi peluang keuntungan dan pengembalian kredit. Konstruksi oven akan lebih sederhana jika digunakan kerangka kayu dan tanpa kolektor surya.Kata kunci : Nicotiana tabacum, L, tembakau, prosesing, oven, portabel- horisontal, kolektor surya, analisis energi, mutu krosok, aspek ekonomi ABSTRACT Engineering of horizontal-potabel curing-barn of Virgi¬ nia tobacco curingEngineeing of the horizontal-portable curing-barn (4m x 8m x 4m) of Virginia tobacco curing lo produce Virginia fc (flue-cured) tobacco conducted in Indonesian Tobacco and Fibers Crops Research Institute, Malang, Indonesia. The curing-bam construction consisted of portable isolation wall, metal frame from L and U iron-bar and flat solar collector which was installed in curing-bam roof. Bros buner and air indirect heating system were used. This curing-bam was tested in East Lombok, West Nusa Tenggara on virgina tobacco harvested in August to October 2002. This horizontal-portable curing-bam was expected to be able to96 produce the homogen temperature and humidity in all space of the curing- bam so that it is suitable for curing the harvesting tobacco leaves which high percentage of mature leaves (90-95%), fuel efficienct and liable to be constructed near tobacco plant area, so that it can decrease the transpotation cost and postharvest damage. The result of the research showed that there were significant difference between above-space and lower-space, let-space and right-space, even it was only 2.53°C and 2.30°C, respectively. The same position for air humidity, 4.55% and 3.64%, respectivelly. Kerosene fuel consumption 1.09 I each kg cured- leaves or 1.16 I each kg cured leaves if without lat solar collector, lower than farmers conventional curing-bam which consumpt 2.39-2.80 I each kg cured-leaves. Flat solar-collector contributed 952 625 Id only or 5.80% of kerosene consumption or equal lo 28.24 I kerosene, even though this equipment needed 17.16% of total curing-bam in vestal ion Heat conduction lost through the portable wall only 3.57%, lower than brickwall conventional curing-bam which reach 12.7-16.0%. Result of the cured-leaves grading gave the average price Rp 12 275,- each kg cured- leaves and economic analysis showed that BC-ratio-1.29, NPV-41 962 590 and IRR=51.83% or still gave the profit chance and ability to pay the capital interest. Result of simulate calculation by simple construction curing-bam, Rp. 8 000 000 price each unit, still gave BC-ratio above one percent. This indicated the profit chance and ability to pay the capital interest. In this simulate calculation three price of cured-leaves and kerosene was used, as followed Rp. 10 000, Rp. 12 500, and Rp. 15 000 each kg cured leaves, and Rp. I 000, Rp. 1 250 and Rp. I 500 each liter of kerosene, respectivelly. The construction of the curing-bam will be simpler if using wood frame and without lat solar collector.Key words : Nicotiana tabacum, L., tobacco, processing, oven, horizontal- portable curing-bam, lat solar-collector, energy analysis, cured-leaves grade, economical-aspec
UJI ADAPTASI VARIETAS DAN GALUR KENAF (Hibiscus cannabinus L.) DI LAHAN PASANG SURUT KALIMANTAN TENGAH
Get PDFABSTRAKPotensi lahan pasang surut Kalimantan Tengah cukup luas yaitu 5,5juta hektar dimana sebagian dapat dikembangkan dengan tanaman kenaf.Penelitian uji adaptasi varietas dan galur kenaf dilaksanakan di lahanpasang surut desa Samuda, kecamatan Mentaya Hilir Selatan, KabupatenKotawaringin Timur, Kalimantan Tengah. Perlakuan berupa varietas/galurkenaf yang terdiri dari dua varietas (Hc G-4 dan Cuba 108/II) dan empatgalur hasil persilangan (Hc 85.9.75; Hc 85.9.40. 1; Hc 85.9.42; Hc85.9.66.1), yang diatur dalam rancangan acak kelompok dengan tigaulangan. Pengamatan dilakukan terhadap tinggi tanaman dan diameterbatang pada umur 40, 75 dan 105 hari setelah tanam terhadap 10tanaman acak per petak, bobot segar biomasa, bobot serat per petak, danbobot kering akar adventif. Hasil penelitian menunjukkan bahwa dua galurhasil persilangan yaitu Hc 85.9.661 dan Hc 85.9.75 mempunyai adaptasicukup bagus dengan tinggi tanaman akhir masing-masing adalah 265,25cm dan 260, 25 cm serta diameter batang masing-masing adalah 2,17 cmdan 2,10 cm. Hasil serat tertinggi masing-masing sebesar 2,40 dan2,30 ton/ha, sementara varietas Hc G-4 mencapai 2,25 ton/ha.Kata kunci : Kenaf, Hibiscus cannabinus L., varietas, galur, adaptasi,lahan pasang surut, Kalimantan TengahABSTRACTAdaptation test of kenaf (Hibiscus cannabinus L.) varieties and lines attidal swamps land, Central KalimantanThe area of tidal swamps In Central Kalimantan is approximately5.5 million hectare and parts of that area can be developed by kenaf plant.The adaptation test of several kenaf varieties and lines was conducted inSamuda Village, Mentaya Hilir Selatan District, Kotawaringin Timur,Central Kalimantan. The experiment used a randomized block design withthree replications and six treatments consisting of two kenaf varieties (HcG-4 and Cuba 108/II) and four kenaf lines (No 85.9.75; No 85.9.40.1; No85.9.42; No 85.9.66.1). Parameters observed were plant height, stemdiameter at 40, 75 and 105 days after planting, on 10 random plantsperplot, fresh biomass, dried fiber, and dried adventive root weight. Theresults of this experiment showed that two lines, namely Hc 85.9.66.1and Hc 85.9.75 obtained the optimal vegetative growth as their plantheight and stem diameter at harvesting time reached 265.25 cm and260.25 cm, 2.17 cm and 2.10 cm respectively. The fiber yields of the twolines were 2.40 and 2.30 ton/ha respectively, while the control line Hc G-4was only 2.25 ton/ha.Key words : Kenaf, Hibiscus cannabinus L., variety, line, adaptation,tidal swamps land, Central Kalimanta
KOMPATD3ILITAS KOMBINASI //aNPV DAN SBM SERTA PENGARUHNYA TERHADAP MORTALITAS DAN AKTIVITAS BIOLOGI PENGGEREK BUAH KAPAS Helicoverpa armigera HUBNER
No full textBanyak cara dapat dilakukan untuk meningkatkan efektivitas HaNPV terhadap serangga hama sasaran. Efektivitas //aNPV terutama daya bunuhnya, dapat ditingkatkan dengan cara mengkombinasikan HaNPV dengan metode pengendalian hama lain yang pengaruhnya dapat menurunkan kckebalan tubuh serangga, misalnya insektisida nabati scrbuk biji mimba (SBM). Disamping untuk meningkatkan efektivitas, kombinasi yang sinergis antara HaNPV dan SBM juga bcrmanfaat untuk substitusi //aNPV yang produk komcrsialnya masih terbatas. Penelitian kompa- tibilitas kombinasi //aNPV dan SBM seta pengaruhnya terhadap mortalitas dan aktivitas biologi larva penggerek buah kapas H. armigera dilaksanakan di laboratoium Hama Balai Penelitian Tanaman Tembakau dan Serat Malang, mulai Maret hingga Juli 2002. Tujuannya adalah untuk mengetahui kompatibilitas dan kemanjuran kombinasi //aNPV dan SBM, seta mengetahui dampak interaksinya terhadap aktivitas biologi penggerek buah kapas //. armigera. Perlakuan yang digunakan adalah kombinasi //aNPV dan SBM berdasarkan konsentrasi subletal dan letal, yaitu: (I) Kontrol (tanpa perlakuan), (2) SBM(LC25), (3) SBM(LC,0), (4) //aNPV(LC25), (5) //aNPV(LC50), (6) /YaNPV(LC25) + SBM(LC2S), (7) /YaNPV(LC25) + SBM(LC50), (8) //aNPV(LC30) + SBM(LC2S), (9) //aNPV(LCjo) + SBM(LCjo). Setiap perlakuan disusun berdasarkan rancangan acak kelompok dengan empat kali ulangan. Hasil penelitian menunjukkan bahwa analisis intcraksi antara //aNPV dan SBM pada berbagai kombinasi konsentrasi umumnya menunjukkan sifat aditif dan sinergis. Kombinasi konsentrasi yang berinteraksi sinergis adalah //aNPV(LCso) t-SBM(LCso) yang menyebabkan mortalitas larva H. armigera ± 80%. Penurunan bobot larva maupun perpanjangan umur stadia larva tcinfeksi secara cfektif dipengaruhi oleh semua perlakuan //aNPV dan SBM, baik individu maupun kombinasi.Kata kunci: Aditif, //aNPV, H. armigera, kompatibilitas, mortalitas, serbuk biji mimba, SBM, sinergisABSTRACTCompatibility of HaNPV and SBM combinations and its effects on the mortality and biological activities of cotton bollworm Helicoverpa armigera HubnerMany ways to increase the effectiveness of //aNPV against insect pests. Combination of //aNPV and other control method, namely using neem seed powder (SBM) which reduced the insect immunity system, was one way to increase the effectiveness of//aNPV. Synergistic combination of SBM to //aNPV not only increased the effectiveness of insect control but SBM itself could also substitute //aNPV which was unavailable commercially. The study was caried out in the Entomology Laboratory of Indonesian Tobacco and Fiber Crops Research Institute (ITOFCRI) in Malang rom March to July 2002. The objective was to ind out the compatibility and efficacy of //aNPV+SBM combination against cotton bollworm and its impacts to larval motality and biological activities. The treatment tested were combinations of //aNPV+SBM based on both sublethal (LCjj) and lethal (LC50) concentrations, viz. (1) Control (untreated), (2) SBM(LC2!), (3) SBM(LCjo), (4) //aNPV(LC23), (5) //aNPV(LC50), (6) //aNPV(LC25) 4 SBM(LC25), (7) //aNPV(LC2J) + SBM(LCso),(8) //aNPV(LC3o) + SBM(LC2S), (9) //aNPV(LCj0) + SBM(LCjo). Each treatment was arranged in a randomized block design (RBD) with four replications Results showed that the combinations of HaNPV and SBM at different concentrations proved to be additive and synergistic interaction. The synergistic interaction was signiicant when //aNPV(LC,o) was combined with + SBM(LC50) with caused ± 80% of larval motality. Reducing in larval weight and prolong the larval age were effectively influenced by HaNPV and SBM either alone or combination.Key words: Additive, compatibility, HaNPV, //. armigera, motality neem seed powder, SBM, synergistic interactio
PENETAPAN KERAGAMAN GENETIK NILAM (Pogostemon sp.) HASIL FUSI PROTOPLAS DENGAN TEKNIK RAPD
No full textKeragaman genetik dan kckerabatan tanaman nilam hasil fusi protoplas antara Nilam Jawa (Girilaya) dan Nilam Aceh (Sidikalang dan TT 75) dianalisis dengan menggunakan penanda RAPD. Bahan tanaman yang digunakan dalam penelitian ini adalah 9 genotipa yang tcrdiri dari 3 tetua dan 6 tanaman hibrida somatik (9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 dan 9 II 10). Primer yang digunakan dalam analisis tcrdiri atas 5 primer acak yaitu OPD 03, OPD 20, OPH 09, OPH 19 dan Abi 117.17. DNA dickstraksi dengan metode OROZCO-CASTJLLO et al. (1994) yang sudah dimodifikasi. Konsentrasi DNA ditetapkan dengan metode sambrook el al. (1989) dengan pcrbandingan kuantifikasi spektrofotometrik. Koefisien kemiripan dan kckerabatan antar genotipa dianalisis dengan menggunakan program NTsys ver. 1.80 dan UPGMA untuk menentukan sidik gerombol dan dendogram. Hasil analisis menunjukkan bahwa koefisien kemiipan dari amplifikasi DNA dengan 5 primer acak dari ke- 9 genotipa berkisar antara 0.48-1.0. Berdasarkan niatrik jarak genetik, kescmbilan genotipa tanaman yang diuji membentuk 2 kelompok besar yaitu kelompok I, tetua Girilaya (Nilam Jawa) dan kelompok II tcrdiri dari tetua Sidikalang dan TT 75 (Nilam Aceh) serta hibrida somatik. Kelompok II, tcrbagi menjadi dua sub kelompok yaitu sub kelompok I (9 II 33 dan 9 II 7) dan sub kelompok II yang tcrdii dari sub-sub kelompok II-I (9 II 21, S, TT 75) dan sub-sub kelompok II-II (2 IV 8, 9 IV 14, 9 II 10).Kata kunci: Pogostemon sp., fusi protoplas, keragaman genetik, RAPD ABSTRACTS Assessment of genetic variability of patchoulli (Pogostemon sp.,) derived from protoplast fussion using RAPD Somatic hybrids of Pogostemon heyneaneus (cv. Girilaya) X P. cablin (cv. Sidikalang and TT 75) were tested for their genetic variability and relationship. The somatic hybrids tested were 9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 and 9 II 10. DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al. (1994) and quantified spectrophotometrically according to SAMBROOK el al. (1989). Five random primers, OPD 03. OPD 20, OPH 09. OPH 19 and Abi 117.17, were applied to amplify the extracted DNA. The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. Index of similarity among genotypes were calculated by using NTsys ver. 1.80 program. Then, cluster analyses to perform dendogram were achieved based on similarity estimates by using the Unweighted Pair-Group Method Arithmetic Average (UPGMA). Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0.48 to 1.0. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli (Sidikalang and TT 75), and the somatic hybrids. The second group was then classified into 2 minor groups. First group consisted of somatic hybrids nos. 9 II 33 and 9 II 7, while the second were classified into 2 groups which consisted nos. 9 II 21, S, TT 75 and nos. 2 IV 8, 9 IV 14 and 9 II 10.Key words : Pogostemon sp., protoplast fusion, genetic variability, RAP
DETERMINASI NEMATODA PARASIT Aphelenchoides sp. PENYEBAB PENYAKIT HAWAR DAUN SAMBILOTO (Andrographis paniculata)
Get PDFABSTRAKNematoda hawar daun Aphelenchoides sp. telah dilaporkanmenyerang dan menyebabkan gugur daun pada sambiloto (Andrographispaniculata (Burm. f.) Wall. ex Nees) (Acanthaceae). Penelitian yangbertujuan untuk mengindentifikasi spesies Aphelenchoides sp. tersebuttelah dilakukan di laboratorium, rumah kaca dan kebun percobaan BalaiPenelitian Tanaman Obat dan Aromatik pada tahun 2005-2006.Identifikasi dilakukan dengan mengamati gejala khas pada tanaman sakitdan karakteristik morfologi nematoda secara mikroskopik pada preparatsemi-permanen Aphelenchoides sp. yang difiksasi dengan larutantriethanolamine formaldehyde (TAF). Gejala khas hawar daun merupakanbercak klorotik yang meluas yang kemudian berubah berwarna kehitamanatau kadang-kadang keunguan yang dibatasi tulang-tulang daun. Secaramikroskopik bentuk tubuh betina Aphelenchoides sp. ramping denganpanjang tubuh berkisar 0,46 – 0,70 mm dan lebar tubuh rata-rata 15 µm;daerah vulva terletak 2/3 dari panjang tubuh diukur dari bagian anterior;stilet ramping, panjangnya 10 µm dengan “basal knobs” kecil tetapi jelas;metakorpus besar, menempati ¾ atau lebih dari lebar esophagus; daerahbibir tampak halus, menonjol, dan bagian depannya rata, dengan konturhampir menyatu/bersambung dengan kontur tubuh; ujung ekornyaberbentuk kerucut tajam memanjang dengan ujung meruncing seperti duritumpul. Bentuk tubuh jantan pada dasarnya sama dengan ukuran danbentuk betinanya; ekor agak melengkung kearah 45º - 90º ketika dalamkeadaan relaks dan bentuk ujungnya meruncing seperti duri tumpul; spikulberbentuk duri mawar (“rose-thorne”). Persentase jumlah jantan dalamsatu populasi umumnya banyak berkisar 34,7 – 38,9% dari populasiseluruhnya (jantan dan betina). Karakter-karakter gejala serangan,morfologi nematoda, dan ratio jantan-betina tersebut merupakan karakterspesies Aphelenchoides fragariae (RITZEMA BOS, 1891) CHRISTIE1932. Deteksi adanya spesies nematoda A. fragariae merupakan yangpertama kali dilaporkan di Indonesia, dan sambiloto sebagai inang A.fragariae juga pertama kali dilaporkan baik di Indonesia maupun di dunia.Kata kunci : Sambiloto, Andrographis paniculata, Aphelenchoidesfragariae, identifikasi, nematoda parasitABSTRACTDetermination of parasitic nematode Aphelenchoides sp.causing leaf blotch disease of sambiloto (Andrographispaniculata)Leaf blotch disease by parasitic nematode Aphelenchoides sp. havebeen reported infected and causedleaf drops on sambiloto (Andrographispaniculata (Burm. f.) Wall. ex Nees) (Acanthaceae). Experiments ofspecies determination of the nematode have been carried out inlaboratorium, greenhouse and fields of IMACRI during the year 2005-2006. Identification were done by observing its typical symptoms of theinfected plants caused by the nematode and its nematode morphologicalcharacteristics microscopically on the semi-permanent preparats fixed byTAF (triethanolamine formaldehyde) solution. Typical symptoms of leafblotch were began as chlorotic vein-deliminated areas which later changedto light brown, then dark brown and finally black; or sometimes purplishunder field condition. Microscopically, the female was slender, 0.46 –0.70 mm long and 15 µm width in average, the vulval region cited about2/3 of the body length sized from anterior part; spear slender, 10 µm longwith small and distinct basal knobs; large metacorpus occupying ¾ ormore of the width of the esophagus; lip region almost continuous withbody contour; the tail tip was elongate-conoid ending in a simple bluntspike. The male was abundant and essentially similar to size and shape ofthe female; tail arcuate through 45º to 90 when relaxed, with a simpleblunt terminal spine; spicules rose-thorn shaped. Those describedsymptoms and morphological characters mentioned above were the typicalcharacters of the species Aphelenchoides fragariae (RITZEMA BOS,1891) CHRISTIE 1932. Detection of species A. fragariae was the firstreport in Indonesia; and sambiloto as the natural host of A. fragariae wasthe first report in Indonesia and internationally.Key words: King of bitter, Andrographis paniculata, Aphelenchoidesfragariae, parasitic nematode, identificatio
KEMAJUAN GENETDX PADA DUA VARIETAS BARU KAPAS, KANESIA 8 DAN KANESIA
No full textTujuan utama pemuliaan kapas di Indonesia adalah meningkatkan produktivitas dan kualitas serat dalam upaya meningkatkan pendapatan petani dan memperbaiki mutu benang tcnun seta kualitas tekstil yang harus bersaing di pasar internasional. Scjumlah enam persilangan telah dilakukan antara dua varietas dai India. I.RA 5166 dan SRT-1 dengan dua varietas dai Amerika Serikat, Dcltapine 55 dan Deltapinc Acala 90 dan satu vaietas dai Australia, Siokra. Seleksi individu, seleksi galur dan seleksi individu dalam galur dilaksanakan pada generasi F2 sampai F5 berdasarkan jumlah buah, tingkat kerusakan daun terhadap Sundapteryx biguttula. dan mutu serat; semua proses di atas dilakukan pada kondisi lahan tadah hujan, dan tanpa penggunaan insektisida terhadap tanaman; dari proses di atas diperoleh 12 galur harapan. Sejumlah 13 percobaan dilakukan antara tahun 1993 sampai dengan 2001 untuk mengamati kcragaan galur-galur baru tersebut; pengujian dilakukan di Jawa Timur dan Sulawesi Selatan, menggunakan teknik-teknik penelitian standar. Dengan proscdur ini dapat diidcntifikasi beberapa galur yang menunjuk¬ kan perbaikan serenlak hasil dan kualitas serat kapas. Beberapa penelitian juga dilakukan untuk mcngcvaluasi tanggap galur-galur tersebut pada tumpangsari dengan kedelai dan kacang hijau di Jawa Timur. Dua galur, 88003/16/2 dan 92016/6 (sudah dilepas dengan nama vaietas Kanesia 8 dan Kanesia 9 pada bulan Juni 2003), menunjukkan produktivitas dan kualitas serai yang lebih linggi. Rata-rata, kedua vaietas menghasilkan 1.85 ton dan 191 ton kapas berbiji per hektar atau 8-12% lebih tinggi dai hasil vaietas Kanesia 7 yang sudah dilepas sebelumnya. Persentase serat 35.2%, kekuatan serat berkisar antara 22.6-24.7 gram tex'1, serat lebih panjang dan berkisar 29.2-30.3 mm sedangkan angka mikroncr lebih rendah yang menyatakan bahwa serat lebih halus. Semua perbaikan di atas menunjukkan perbaikan mutu serat. Kanesia 8 dan Kanesia 9 juga menunjukkan peningkatan ketahanan terhadap Sundapteryx biguttula dan komplcks hama kapas. Kanesia 8 dan Kanesia 9 kurang kompctitif dalam tumpang sari dengan kedelai jika dibandingkan dengan Kanesia 7. Pada tumpang sari dengan kacang hijau Kanesia 8 juga mengalami kehilangan hasil yang tinggi, sedangkan Kanesia 9 menunjukkan toleransi yang tinggi dalam kompctisi dengan kacang hijau. Pelepasan Kanesia 8 dan Kanesia 9 akan memberikan pilihan varietas yang lebih banyak bagi petani dan perusahaan pemintalan untuk menyesuaikan dengan produk akhirnya.Kata kunci : Gossypium hirsutum, prosedur pemuliaan, produktivitas, kualitas serat, Sundapteryx biguttula, tumpangsari ABSTRACT Genetic improvement on two new cotton varieties, Kanesia 8 and Kanesia 9The main objective of cotton breeding in Indonesia is to improve productivity and fiber quality which is aimed to increase farmers' income and to make beter yam and textile quality that has to compete in international market Six crosses were made between two Indian varieties, LRA 5166 and SRT-1 with two USA vaieties, Deltapine 55 and Deltapinc Acala 90 and one Australian variety, Siokra. Individual plants, lines and individual within lines were selected on F2-F5 generations based on boll- counts, leaf-damage by jassids and fiber traits, those were conducted under rainfed and insecticide-ree condition; twelve promising lines were produced from this process. A total of 13 trials were carried out to observe performance of these new lines during 1993 to 2001; those were located in East Java and South Sulawesi using the standardized experimental techniques. By these procedures make it possible to identify several breeding lines showing simultaneous improvement in yield and fiber quality. Several tests were also made to evaluate response of those lines under intercropping with soybean and mungbean, which were located in East Java. Two breeding lines, 88003/16/2 and 92016/6 (those have been released as Kanesia 8 and Kanesia 9 in 2003), showed higher productivity and fiber quality. In average, these new vaieties produced 1.85 and 1.91 ton ha'1 seed cotton respectively or 8 to 12% higher than those on Kanesia 7, the previously released vaiety. Lint turn-out was 35.2% fiber-strength was varied from 22.6 to 24.7 gram tex'1 , fiber lengths ranged from 29.2 to 30.3 mm with lower micronaire-valucs indicating better fiber-ineness. All of those improvements represented a trend toward a higher quality iber. Kanesia 8 and Kanesia 9 also showed a slight improvement in resistance to jasssids and insect pest-complex. Kanesia 8 and Kanesia 9 performed lower competitive ability under intercropping with soybean in comparison with Kanesia 7. Under intercropping with mungbean Kanesia 8 also suffered high yield loss, wherein Kanesia 9 showed good tolerance to mungbean. The release of Kanesia 8 and Kanesia 9 is expected to give a broader choice for the cotton growers and spinning-mills to match with their inal product.Key words: Coton (Gossypium hirsutum), breeding procedure, productivity, liber quality, Sundapteryx bigullul. inter¬ cropping
KADAR FOSFOR DAUN JARAK PAGAR (Jatropha curcas L.) DAN KORELASINYA DENGAN FOSFOR TANAH TERSEDIA DARI BEBERAPA METODE EKSTRAKSI
Get PDFABSTRAKPercobaan untuk mengetahui kesesuaian beberapa metoda uji Ptanah untuk tanaman jarak pagar (Jatropha curcas L.) dan pengaruhberbagai dosis pupuk P terhadap kadar P daun dan berat kering tanamanterhadap jarak pagar telah dilakukan pada tanah Ultisol Citayam, Bogor.Percobaan dilakukan di Rumah Kaca Balai Penelitian Tanaman Rempahdan Aneka Tanaman Industri Pakuwon, Jawa Barat, dari bulan September2006 sampai Juni 2007. Perlakuan terdiri atas dosis P (SP-36), yaitu 0, 50,100, dan 150 mg P O2 5 /kg tanah, yang disusun dalam Rancangan AcakLengkap (RAL) dengan empat ulangan. Hasil penelitian menunjukkanbahwa kadar P daun dan berat kering tanaman jarak pagar meningkatsejalan dengan dosis pupuk P yang diberikan. Peningkatan dosis pupuk Pjuga diikuti oleh peningkatan kadar P tanah yang diekstraksi denganmetoda Olsen-P, Bray-1 P, dan HCl 25%. Nilai-nilai kadar P tanah tersediayang diukur dengan ketiga metoda uji P tanah tersebut mempunyai hubungan yang nyata dengan kadar P tanah yang diekstraksi dengan determinasi (R 2 ) tertinggi ditunjukkan oleh persamaan regresi dari nilai-nilai P tanah dengan metoda Bray-1 P (R 2 = 0.92) dibandingkan denganmetoda Olsen-P dan HCl 25%, yang menunjukkan bahwa metoda ujitanah Bray-1 P lebih sesuai untuk penyusunan rekomendasi pemupukan Puntuk tanaman jarak pagar di tanah Ultisol.Kata kunci: Jatropha curcas L., P daun, metode uji P tanah, hubunganantara P daun dan P tanahABSTRACTA study was conducted to compare the suitability of several soil Ptest methods for physic nut (Jatropha curcas L.) and to determine theeffect of various doses of P fertilizer on the leaf P content and the plant drymatter on Ultisol Citayam, Bogor. The experiments were carried out in aglasshouse from September 2006 up to July 2007. The employedtreatments, namely 0, 50, 100, and 150 mg P O2 5 /kg soil (in form of SP-36),were arranged in a Completely Randomized Design (CRD) with fourreplications. The results showed that the leaf P content and plant drymatter of physic nut on Ultisol increased in line with increasing doses of Pfertilizer applied. Increase of P fertilizer P rates was also followed by theincrease of soil P concentrations extracted by the three soil P test methods(Olsen-P, Bray-1 P, and HCl 25%). The values of available soil P concentration measured by the three soil P test methods had significant relationship with leaf P contents. The highest coefficient of determination (R 2 ) showed by the regression equation of available soil P concentrationmeasured by the Bray-1 P (R 2 = 0,92) compared to those measured by theOlsen-P and HCl 25% methods, indicated that the Bray-1 P method islikely more suitable to be used in establishing P fertilizerrecommendations for the physic nut in Ultisol.Key words: Jatropha curcas L., leaf P, soil P test methods, relationshipbetween soil P and leaf P content