Jurnal Penelitian Tanaman Industri
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ANALYSIS OF GENETIC STABILITY OF MICROPROPAGATED SUGARCANE IN DIFFERENT SUBCULTURE FREQUENCIES USING SSR MARKER
Get PDFIn vitro technique is an effective method to produce high quality and uniform sugarcane seedlings. This study was aimed to determine genetic stability based on SSR marker analysis of six varieties of sugarcane subcultured in regeneration media. It was conducted at the ICABIOGRAD Molecular Biology Laboratory, Bogor, from May 2015 to October 2016. Six sugarcane varieties (PS 862, PS 865, PS 881, PSJK 922, TK 386, and GMP 3) derived from apical shoot explants were subcultured on MS regeneration media enriched with 0.3 mg/l BAP; 0.5 mg/l IBA; and 100 mg/l PVP, for 3, 6 and 9 times. Sugarcane DNA was extracted using the CTAB method; then, the genetic stability was analyzed using 20 pairs of SSR primers. Data were analyzed in groups using the UPGMA method in the SAHN subprogram available on NTSYS software. The results showed that five sugarcane varieties (PS 865, PS 881, PSJK 922, TK 386, and GMP 3) subcultured up to nine times on the regeneration media remained genetically stable with similarity coefficient to their mother plants value more than 0.94. However, PS 862 variety had genetically unstable after the sixth and the ninth subcultures, the similarity coefficient value to its mother plant was only 0.64, indicated that it experienced somaclonal variations. The study concluded that the in vitro shoots of the other varieties were more genetically stable during subcultures compared to PS 862 sugarcane variety based on SSR marker analysis. Further study is needed to find out the cause of genetic changes in PS 862.Keywords: Saccharum officinarum, apical shoots, in vitro propagation
KARAKTERISASI Erwinia chrysanthemi PENYEBAB PENYAKIT BUSUK BAKTERI PADA DAUN LIDAH BUAYA (Aloe vera)
No full textPenyakit busuk (soft rot) pada daun tanaman lidah buaya (Aloe vera) disebabkan oleh F.rwima chrysanthemi pertama kali dilaporkan di Kcpulauan Kaibia tahun 1992. Pada awal tahun 2001, gejala pcnyakil busuk daun juga ditemukan pada tanaman lidah buaya di Semplak, Jawa Barat. Mengingat kerusakan yang ditimbulkan berupa pembusukan pada daun dan pangkal batang yang parah dalam waktu singkat, maka diduga penyebabnya cukup ganas dan dikhawatirkan dapat menjadi kendala pengembangan tanaman lidah buaya yang akhir-akhir ini sedang banyak diminati. Tujuan penelitian ini adalah mengidcntifikasi penyebab penyakit tersebut. Penelitian dilakukan di Laboratorium Bakteri Balai Penelitian Tanaman Rempah dan Obat, dari bulan April - Agustus 2001. Contoh daun lidah buaya sakit berasal dari kebun petani di Semplak, Bogor. Setelah melalui pengamatan gejala penyakit diikuti dengan prosedur isolasi dan pemumian patogen. maka diperoleh isolat kultur baktei yang bentuknya bulat dan pinggirnya tidak rata serta berwarna putih pada medium sukrosa pepton agar. Kultur baktei tersebut bersifat patogenik dan menimbulkan gejala penyakit sama seperti di lapangan, setelah diinokulasikan melalui pelukaan pada daun lidah buaya. Hasil karaktcrisasi morfologi, kultur dan biokimia isolat baktei tersebut menunjukkan sifat ncgatif untuk pewamaan Gram, pigmen fluorescn, oksidasc, dan produksi asam dari unsur karbon laktosa dan dulsitol. Sedangkan karaktcr positif diperoleh dai pengujian oksidasi/fermentasi, lesitinase, pembusukan jaringan umbi kentang, sensitivitas terhadap eitromisin, pertumbuhan pada suhu 37° C, NaCI 5%, serta menghasilkan asam pada medium mengandung manitol Inokulasi pada umbi ubi jalar menyebabkan pembusukan dalam waktu yang singkat. Berdasarkan data tersebut dapat disimpulkan bahwa penyebab penyakit busuk daun pada tanaman lidah buaya di Semplak adalah Erwinia chrysanthemi.Kata kunci: Aloe vera, busuk daun, Erwinia chrysanthemi, Bogor ABSTRACT Characteristics o/Envinia chysanthemi causing bacterial soft rot ofAloe (Aloe VeraJThe bacterial sot rot of aloe, caused by Erwinia chrysanthemi, was first identified in Caibbean Island in 1992. In early 2001, similar symptoms were found on the aloe plants grown in Semplak, Bogor, West Java. Based on its symptom and progressively spread, especially on the leaf and basal stem, it appeared that the disease was serious and therefore threatened the current development of die plants. This study was conducted in the laboratory of the Research Institute for Spice and Medicinal Crops, Bogor, in April - August 2001. The objective of the study was identifying the cause of the sot rot disease of aloe in Semplak, Bogor. Diseased leaves of aloe were obtained from Semplak. Following the examination of the symptoms, isolation and purification of he causal agent, the bacteial isolates were found. They were round, white colony characteistics on sucrose peptone agar medium. The isolate was pathogenic and caused similar disease symptoms following the artificial inoculation on the wounded aloe leaf. Based on the morphological, cultural, and biochemical analyses of the isolates, it was found that the isolates gave negative reactions for die following characteistics: Gram staining, production of fluorescent pigment, oxidase, and production of acid reaction from lactose and dulcitol. The isolates, on the other hand, gave positive reaction from: oxidation/fermentation, IcciOiinase. and maceration of potato and sweet potato, sensitive to crythromycin, growth at 37 °C, and growth on agar medium containing NaCI 2%, as well as acid production from manmtol. Based on these data, it can be concluded that the pathogen causes sot rot of leaf of aloe in Semplak is Erwinia chrysanthemi. This is the irst repot on (he finding of die disease in Indonesia. More attention is required to stop the spread of the disease.Key words: Aloe vera, sot rot. Erwinia chrysanthemi, Bogo
KARAKTERISTIK NANOEMULSI MINYAK SAWIT MERAH YANG DIPERKAYA BETA KAROTEN
No full textABSTRAKMinyak sawit merah (Red palm oil/RPO) dan β-karoten tidak larutdalam air sehingga sulit diaplikasikan ke dalam produk pangan. Salah satupendekatan untuk meningkatkan kelarutan RPO dan β-karoten adalah emulsifikasi. Penelitian ini bertujuan untuk mendapatkan nanoemulsi RPOdiperkaya β-karoten yang stabil. Penelitian dilaksanakan di LaboratoriumSEAFAST CENTER IPB dari Januari–September 2013. Pada penelitiantahap pertama, nanoemulsi disiapkan melalui tahap-tahap: pengayaan RPOdengan β β-karotenmenggunakan HPH (High Pressure Homogenizer) pada tekanan 34,5 MPadengan 10 siklus. Rasio RPO dan air dalam emulsi adalah 5 : 95; 7,5 :92,5; dan 10 : 90 (b/b), dan persentase Tween 80 sebagai pengemulsiadalah 2,5; 5,0; 7,5; dan 10% (b/b) dari total emulsi. Pada tahap kedua,nanoemulsi disiapkan dengan persentase RPO: 2, 4, dan 6% (b/b) danpengemulsi 1,5; 3,0; dan 4,5% (b/b) dari total emulsi. Hasil penelitiantahap pertama menunjukkan nanoemulsi yang dibuat dengan rasio RPO :air = 5 : 95 dan 7,5 : 92,5 serta pengemulsi 5% (b/b) menghasilkan emulsidengan ukuran droplet 115,1 sampai 145,2 nm dan stabil. Nanoemulsiyang dihasilkan dari penelitian tahap kedua memiliki ukuran droplet 94,9sampai 125,5 nm, dan kadar β-karoten antara 47,6 sampai 130,9 mg/l.Ukuran droplet nanoemulsi yang kurang dari 125 nm dapat dihasilkandengan formula rasio RPO dan pengemulsi kurang dari 2,0.Kata kunci: minyak sawit merah, β-karoten, nanoemulsi, homogenizerABSTRACTRed palm oil (RPO) and β-carotene are insoluble in water. It makescan be used to improve RPO and βThis research is aimed to produce stable RPO nanoemulsion enriched withβ-carotene. The research was conducted in the SEAFAST CENTERLaboratory, Bogor Agriculture University from January to Septemberfollowing steps, i.e. enrichment of RPO with βusing a high pressure homogenizer at a pressure of 34.5 MPa in 10 cycles.The ratio of RPO and water in the mixture were 5 : 95; 7.5 : 92.5; and 10 :10% (w/w) of the total emulsions. In the second stage, nanoemulsionswere prepared on various RPO percentage of 2, 4, and 6% (w/w) andhad a droplet size from 115.1 to 145.2 nm and stable. Nanoemulsions wereresulting from the second stage had droplet size from 94.9 to 125.5 nm,and β-carotene content were 47.6 to 130.9 mg/l. Droplet size ofnanoemulsions is less than 125 nm. It can be produced with RPO andKey words: red palm oil, β-carotene, nanoemulsion, homogenize
KERAGAAN PERBENIHAN JAHE DI JAWA BARAT
No full textUntuk memperbaiki sistem perbenihan jahe telah dilakukan penelitian terhadap pctani maju di Jawa Barat yang secara tradisional mcnsuplai kebutuhan benih jahe untuk petani disekitarnya atau petani di daerah lain. Penelitian dilakukan dengan metode survei di dua kabupaten, yaitu Majalengka (Kecamatan Banjaran dan Lemah Sugih) dan Kabupaten Sukabumi (Kecamatan Parakan Salak, Jampang Tengah, Tegallega, dan Waning Kiara). Wawancara dilakukan secara langsung dengan petani maju meliputi kondisi lahan, cara budidaya, panen, dan penanganan benih. Contoh rimpang diambil secara acak dai hasil panen petani kemudian dianalisis kualitasnya meliputi: penampilan fisik, berat rimpang, kadar air, serat, dan pati, serta jenis OPT. Hasil penelitian menunjukkan bahwa tidak ada petani yang secara khusus menanam jahe untuk benih. Lahan pertanaman jahe bcrada pada kisaran kelinggian tempat (dpi) 400-800 m. Sumber benih berasal dari hasil panen tahun sebelumnya Cara penanganan benih ada yang dihampar di atas rak bambu atau di gelar di atas tanah di bawah kolong rumah. Sebelum ditanam, benih diperlakukan tcrlebih dahulu dengan fungisida atau bakterisida. Petani menanam jahe dengan sistem polikultur dengan tanaman lain seperti kacang tanah, jagung, pisang, bawang merah, dan bawang daun. Cara tanam ada yang menggunakan bedengan (umumnya di Majalengka), sedangkan di Sukabumi umumnya tidak menggunakan bedengan, jarak tanam bervariasi antara 20 x 30 cm di dalam baris dan 30-80 cm antar baris. Panen dilakukan setelah tanaman berumur 8-10 bulan Hasil analisis kandungan pati cukup tinggi 42.4 - 56.35%, serat 5.67 - 7.40%, dan kadar air 8.37- 9.80%. Jenis OPT yang paling banyak ditemukan pada contoh benih jahe Aspidiella hartii. Mimegralla, dan Meloidogyne spp., sedangkan Ralstonia solanacearum tidak ditemukan. Hasil penelitian menunjukkan bahwa untuk membentuk suatu sistem produksi benih jahe yang standar maka unsur yang sangat menentukan adalah umur panen harus cukup tua (9-10 bulan), bebas dari OPT, dan penanaman bisa secara polikultur dengan tanaman bukan inang.Kata kunci: Jahe, Zingiber officinale, perbenihan, benih, OPT, hama, penyakit ABSTRACT Performance of indigenous ginger seed systems in West JavaTo improve the system of ginger seed production, a study was conducted on developed ginger farmers in West Java who traditionally supplied ginger seeds to the farmers in the surrounding area or in other area. The research was conducted by survey method in two districts, Majalengka (Banjaran and Lemah Sugih sub districts) and Sukabumi (Parakan Salak, Jampang Tengah, Tegalega and Warung Kiara). Direct dialogue to farmer was used to discuss about land condition, cultivation method, harversting and seed management. The rhizome/seed sample was taken by random from the farmers, then it was analyzed for quality, such physical quality, weight of rhizome, moisture content, fiber, carbohydrate and kinds of pest and deseases. The result of the research showed that there were no farmers who special cultivated ginger for seed. Ginger land area was located about 400-800 m above sea level (asl). Seed source was derived from the ginger seed of previous harvest. The ginger seeds were directly spread on bamboo rack or in the space under the house. Before planting, the seeds were treated with fungicide or bactcricide. The farmers, planted ginger by polyculiurc system with peanut, maize, banana, shallot, and green shallot. Generally, in Majalengka District, the farmers planted their ginger using seed bed method, while in Sukabumi the fanners planted of ginger without seed beds. Plant spacing was 20-40 cm in the row and 30-80 cm between rows. Harvest was done 8-10 months ater planting. Carbohydrate content was relatively high 42.4 - 56.35%, fiber 5.67-7.40% and moisture content 8.37- 9.80%. The majority of pest and disease found in ginger seed were Aspidiella hartii, Mimegralla and Meloidogyne, while the dangerous disease such as Rasllonia solanacearum was not found. Based on the result of research it could be recommended that to develop a standard ginger seed system the harvesting time is 9 to 10 months ater planting, seed are free from disease attack, and the ginger can be cropping system with non hostplant.Key words: Ginger, Zingiber officinale, seed system, seed, pest, diseas
PENDUGAAN AKSI GEN DAN DAYA WARIS KETAHANAN KAPAS TERHADAP Amrasca biguttula
Get PDFABSTRAKAmrasca biguttula merupakan salah satu hama utama kapas yangmampu menurunkan hasil secara nyata. Penggunaan varietas tahan hamasecara genetik merupakan salah satu dari sekian metode pengendalianyang efektif untuk menurunkan kerusakan hama. Penelitian bertujuanuntuk mengetahui aktivitas kerja gen dan daya waris gen yang mengen-dalikan sifat ketahanan terhadap hama pengisap daun A. biguttula.Penelitian dilakukan di Karangploso, Kabupaten Malang, Jawa Timur daribulan Mei sampai Oktober 2006. Pengujian dilakukan terhadap 17genotipe yang terdiri dari (a) tiga varietas tetua jantan berbulu lebat yaituLRA 5166, SRT-1, dan Laxmi; (b) dua varietas tetua betina yangditingkatkan ketahanannya yaitu Kanesia-8 dan Kanesia-9; (c) enamgenotipe generasi F1 hasil persilangan tetua jantan dan betina tersebut diatas, dan d) enam genotipe generasi F2 yang merupakan keturunan darihasil persilangan F1. Percobaan disusun dalam rancangan acak kelompok(RAK), diulang 3 kali. Hasil penelitian menunjukkan bahwa tiga genotipeF1 dari pasangan persilangan Kanesia-8 x LRA 5166, Kanesia-9 x LRA5166, dan Kanesia-8 x Laxmi menunjukkan penampilan gen yangmengatur kelebatan bulu daun bersifat dominansi sebagian negatif.Sedangkan penampilan gen pada pasangan persilangan Kanesia-8 x SRT-1, Kanesia-9 x SRT-1, dan Kanesia-9 x Laxmi adalah dominansi sebagianpositif. Nilai heritabilitas dalam arti luas dari gen yang mengatur kelebatanbulu daun pada empat pasangan persilangan Kanesia-8 x SRT-1, Kanesia-9 x SRT-1, Kanesia-8 x Laxmi, dan Kanesia-9 x Laxmi adalah tinggi,sedangkan dua pasangan persilangan Kanesia-8 x LRA 5166 dan Kanesia-9 x LRA 5166 nilai heritabilitasnya sedang. Korelasi nyata terjadi antarajumlah bulu daun, populasi nimfa dan nilai JRI. Tingkat kehadiranpopulasi nimfa wereng dan nilai JRI sangat dipengaruhi oleh kerapatanbulu daun.Kata kunci : Gossypium hirsutum, Amrasca biguttula, kerapatan bulu,ketahanan, daya warisABSTRACTEstimation of Gene Action and Resistance Heritability ofCotton to Amrasca biguttulaAmrasca biguttula is one of main pests attacking cotton that causessignificant yield loss. The use of resistant varieties is genetically aneffective way to control the pest. An experiment was conducted to studythe activity and heritability of gene(s) responsible for controlling cropresistance to jassid, A. biguttula. The test involved 17 genotypes consistingof (a) three varieties with high trichome density as male parents i.e. LRA5166, SRT-1, and Laxmi; (b) two varieties to be improved their resistanceto jassid as female parents i.e. Kanesia-8 and Kanesia-9; (c) six genotypesof F1 generation resulted from crossing between male and female parents,and d) six genotypes of F2 generation resulted from selfing of genotypes.The test was arranged in randomized block design with three replications.Experimental result showed that the action of gene(s) responsible intrichome density or leaf pubescent of three F1 genotypes i.e. Kanesia-8 xLRA 5166, Kanesia-9 x LRA 5166, and Kanesia-8 x Laxmi were partlynegative dominance, whereas those of F1 genotypes of Kanesia-8 x SRT-1, Kanesia-9 x SRT-1, and Kanesia-9 x Laxmi were partly positivedominance. The heritability of that gene(s) in Kanesia-8 x SRT-1,Kanesia-9 x SRT-1, Kanesia-8 x Laxmi, and Kanesia-9 x Laxmicombinations were high, whereas those in Kanesia-8 x LRA 5166 andKanesia-9 x LRA 5166 combinations were medium. A significantcorrelation was observed between trichome density, nymph population,and JRI value, in which nymph population and JRI were significantlyinfluenced by trichome density.Key words : Gossypium hirsutum, Amrasca biguttula, gene action,heritabilit
PENGGUNAAN AIR KELAPA SEBAGAI ZAT PENGATUR TUMBUH PADA MULTIPLIKASI TUNAS TEMULAWAK (Curcuma xanthorrhiza Roxb.) IN VITRO
Get PDFABSTRAKTanaman temulawak (Curcuma xanthorrhiza Roxb.) merupakansalah satu tanaman obat potensial unggulan yang memiliki khasiatmultifungsi. Rimpangnya yang berkhasiat obat mampu mengobati ber-bagai penyakit seperti kelainan pada hati/lever, kantong empedu, danpankreas. Adanya kecenderungan masyarakat ingin menggunakan pengo-batan dengan bahan alami, menjadikan permintaan benih temulawaksebagai bahan baku obat maupun industri jamu di Indonesia meningkatdengan pesat. Kondisi ini memberi peluang kepada petani sebagaipenyedia bahan tanaman. Upaya penyediaan bahan tanaman secara massaldalam waktu singkat serta bebas hama dan penyakit dapat dilakukanmelalui teknik kultur jaringan. Teknik ini dibatasi oleh tingginya biayaperbanyakan, di antaranya penggunaan bahan kimia. Oleh karena itu perludikaji penggunaan zat pengatur tumbuh (ZPT) yang berasal dari bahanalami (salah satunya adalah air kelapa) sebagai substitusi ZPT sintetik.Penelitian penggunaan air kelapa sebagai ZPT dilakukan di LaboratoriumKultur Jaringan Plasma Nutfah Pemuliaan dan Perbenihan, BalaiPenelitian Tanaman Obat dan Aromatik Bogor, dari bulan Mei sampaidengan bulan Desember 2009. Eksplan berasal dari tunas temulawak sterilhasil perbanyakan sebelumnya. Media yang digunakan adalah mediaMurashige and Skoog (MS) yang dikombinasikan dengan beberapa tarafkonsentrasi air kelapa (0, 5, 10, 15, dan 20%) sebagai substitusi ZPT danair kelapa dengan memakai millipore. Media dibuat padat, sebagaipembanding pada media MS + ZPT kimia yaitu BA1,5 mg/l. Percobaanmenggunakan rancangan acak lengkap dengan 10 ulangan. Parameteryang diuji adalah jumlah tunas, jumlah daun dan jumlah akar. Hasilpenelitian menunjukkan, tanpa komponen kimia, dengan penambah airkelapa pada berbagai konsentrasi pada media dasar MS, berhasilmembentuk tunas, daun dan akar. Jumlah tunas terbanyak didapat padakombinasi media dengan penambahan air kelapa 15% sebanyak 3,4 tunas,jumlah daun 2,2 daun serta jumlah akar terbanyak yaitu sebanyak 13,2akar pada umur 2 minggu. Pada kombinasi media dengan memakaimillipore, tunas terbanyak hanya 2,6 tunas, tetapi tidak berbeda nyatadengan perlakuan kontrol MS + BA 1,5 mg/l, yaitu sama-sama memiliki2,6 tunas, 3,6 daun, dan 15,4 akar.Kata kunci : Curcuma xanthorrhiza Roxb., in vitro, air kelapa, zatpengatur tumbuh, multiplikasi in vitroABSTRACTThe use of Coconut Water as Growth Regulator onMultiplication of Java Turmeric Buds (Curcumaxanthorrhiza Roxb. ) in vitroJava turmeric (Curcuma xanthorrhiza Roxb.) is a potentialmedicinal plant which has many uses. Its rhizome has efficacy to curevarious diseases such as disorder on lever, gall bladder and pancreas.There is a tendency that people want to use therapy by natural materials,increases demand of turmeric seed as raw material of medicine industry inIndonesia. This condition provides a chance to farmers as supplier of plantmaterials. However, up to now, the high need of plant materials causes thelimitation of supply so that their alternatives are needed for providing plantmaterials in maximum number. The part of plant material provision in highnumber and in a short time and free from pests and diseases can beconducted through tissue culture technique. However, this technique islimited by the high cost of multiplication, among others the use ofchemical materials. Therefore, the use of growth regulator originated fromnatural material as substitution of synthetic growth regulator need to beassessed, one of them is coconut water. The experiment was carried out atthe laboratory of Tissue Culture, Germ Plasm, and Plant Breeding,Indonesia for Medicinal and Aromatic Crop Research Institute, Bogorfrom May to December 2009. Explants originated from sterile turmericshoots, product of previous multiplication. Media used was Murashige andSkoog (MS) combined with several concentration levels of coconut water( 0; 5; 10; 15, and 20%) as substitution of growth regulator and coconutwater by using millipore. Solid media was used, as comparison on mediaof chemical MS + was BA1.5 mg/l. The experiment was arranged incompletely randomized design with 10 replications. Parameters observedwere the numbers of shoots, leaves and roots. Results showed that withoutchemical component, by addition of coconut water on variousconcentrations on based media of MS, produced shoots, leaves and roots.The highest shoot number obtained on combination of media and additionof coconut water 15% as many as 3.4 shoots, with the number of leaves2.2 leaves at the age of 2 weeks and the highest roots formed on 15 %coconut water as many as 13.2 roots. Whereas on combination of mediawith millipore, the highest shoots were only 2.6 shoots, however it was notsignificantly different from treatment of control MS + BA 1.5 mg/l, itproduced 2.6 shoots,3.6 leaves and 15.4 roots.Key words : Curcuma xanthorrhiza Roxb., in vitro, coconut water,growth regulator, multiplication in vitr
PERTUMBUHAN DAN PRODUKSI TANAMAN KACANG TANAH (Arachis hipogea) DAN SENGON (Paraserianthes falcataria) DI ANTARA TANAMAN KELAPA DI SUKABUMI JAWA BARAT
No full textPenelitian dilaksanakan pada bulan Januari sampai dengan Desember 2001 di Desa Caringinnunggal, Kecamatan Waluran, Kabupaten Sukabumi, yang bcrada pada kelinggian 250 m di atas permukaan laut, tipe iklim B2 (Oldeman) dan jenis tanahnya Podsolik Merah Kuning. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak kelompok dengan empat ulangan. Perlakuan model polatanam kelapa yang diuji sebagai berikut (1) kelapa + sengon 140 pohon/ha ♦ kacang tanah, (2) kelapa + sengon 280 pohon/ha + kacang tanah, (3) kelapa + sengon 420 pohon/ha ♦ kacang tanah, (4) kelapa + sengon 560 pohon/ha + kacang tanah, (5) kelapa + sengon 700 pohon/ha + kacang tanah dan (6) kelapa monokultur. 'Tujuan dai penelitian ini untuk mcngciahui pertumbuhan dan produksi dari beberapa model polatanam kelapa dengan tanaman sela kacang tanah dan sengon. Pengamatan dilakukan terhadap parameter agronomi meliputi data petumbuhan kacang tanah, sengon dan kelapa seta data produksi kacang tanah. Data yang diperoleh dianalisis dengan menggunakan sidik ragam dan dilanjutkan dengan uji BNJ. Hasil penelitian menunjukkan produktivitas kacang tanah pada polatanam kelapa * sengon 700 pohon/ha + kacang tanah prospektif dengan hasil mencapai 2 183 kg/ha dengan tinggi tanaman 30.25 cm dan polong isi sebanyak 9.65 buah. Sedang untuk tanaman sengon tingginya telah mencapai 312.3 cm dengan diameter 10.57 cm. Adanya tanaman sela diantara kelapa tidak menurunkan pembentukan jumlah daun, jumlah bunga betina dan jumlah tandan tanaman kelapa.Kata kunci: Cocos nucifera, Paraserianthes falcataria, Arachis hipogea, polatanam ABSTRACTGrowth and producion of peanut and albizia between coconut palms in Sukabumi, West JavaThe study was conducted from January to December 2001 at Caringinnunggal, Sukabumi 250 m above sea level with type of climate Bj according to Oldeman and soil type Red Yellow Podsolic. The expeiment was designed as a randomized block in 4 replicates. The cropping system models consisted of : (1) Coconut + albizia 140 trees/ha ♦ peanut (2) Coconut + albizia 280 trees/ha ♦ peanut. (3) Coconut + albizia 420 trees/ha + peanut, (4) Coconut + albizia 560 trees/ha + peanut, (5) Coconut + albizia 700 trees/ha + peanut and (6) Coconut as a control The purpose of this experiment was to know the growth and productivity of several fanning systems with intercrops in between coconut. The results of the research showed that the peanut production in the cropping system of coconut + albizia 700 trees/ha + peanut reached 2 183 kg/ha, peanut plant height 30.25 cm, mature pods 9.65/plant. While Ihe height of albizia plant reached 3)2.3 cm, stem diameter 10.57 cm. The intercrops planted in between the coconut palms did not reduce the number of leaves, the number of female flowers, and (he number of bunches.Key words : Cocos nucifera, Paraserianthes falcataria. Arachis hipogea, cropping system, productio
PENGEMBANGAN TANAMAN JARAK PAGAR (Jatropha curcas L) MENDUKUNG KAWASAN MANDIRI ENERGI DI NUSA PENIDA, BALI
Get PDFABSTRAKDalam upaya memenuhi kebutuhan listrik di daerah terpencil danpulau-pulau kecil, pemerintah meluncurkan kebijakan pengembangan desamandiri energi. Di Nusa Penida, salah satu kawasan di Bali yang terdiriatas 3 pulau kecil, program mandiri energi dirancang dalam bentuk desawisata energi yang diwujudkan dengan pengembangan tanaman jarakpagar (Jatropha curcas L) sebagai penghasil bahan bakar nabati (BBN)untuk subtitusi bahan bakar pembangkit listrik tenaga diesel (PLTD).Analisis kebijakan pengembangan tanaman jarak pagar mendukungkawasan mandiri energi Nusa Penida dilakukan pada bulan Oktober 2007-April 2008. Penelitian ini bertujuan untuk: (1) mengkaji kesesuaian lahandan iklim Nusa Penida untuk pengembangan tanaman jarak, dan (2)melakukan analisis kelayakan finansial usahatani jarak pagar. Kesesuaianlahan dan iklim dianalisis secara deskriptif, sedangkan kelayakan finansialdianalisis berdasarkan kriteria investasi : NPV, B/C, dan IRR. Hasilpenelitian menunjukkan bahwa berdasarkan karakteristik iklim, wilayahNusa Penida termasuk ke dalam kriteria sesuai (S2) untuk pengembangantanaman jarak pagar. Unsur iklim yang menjadi pembatas adalahketersediaan air terutama pada bulan-bulan Agustus, September, danOktober yang merupakan puncak musim kemarau, sehingga waktu panenhanya berlangsung pada bulan Maret-Juli. Peran minyak jarak pagarmensubstitusi solar sebagai bahan bakar PLTD selain ditentukan olehwaktu panen, juga tergantung kepada harga biji jarak pagar yang dapatmemberikan insentif bagi petani untuk mengembangkan tanaman tersebut.Usahatani jarak pagar layak dikembangkan pada tingkat harga minimumRp 2.000/kg biji di tingkat petani.Kata kunci : Jatropha curcas L., kesesuaian lahan dan iklim, kelayakanfinansialABSTRACTPhysic Nut Jatropha curcas Development to SupportLocal Self-sufficient Energy in Nusa Penida, BaliTo fulfill the electricity requirement in remote areas and smallislands, Indonesian government runs the self-sufficient energy villageprogram. In Nusa Penida, an area that consists of three islands in BaliProvince, the program is run by developing Energy Tourism Area (ETA).In this program, physic nut (Jatropha curcas L.) was planted in the ETAand the seeds will be used for bio-diesel to substitute diesel powerelectricity generator fuel. A policy analysis of developing Jatropha curcasplantation in the ETA has been done in the period of October 2007 to April2008. The objectives of this research are : (1) to analyze the land andclimate suitability for planting physic nut, and (2) to analyze financialfeasibility of physic nut farming. The land and climate suitability analyzedby descriptive method. Financial feasibility analyzed by investmentcriteria : NPV, B/C ratio, and IRR. The result shows that the land andclimate in Nusa Penida is suitable (S2) for planting physic nut. The crucialelement of the climate is the availability of the water during dry season inAugust, September and October. The harvest season is in March to July.The role of physic nut as a source for bio-diesel is influenced by theharvest time and the price of physic nut seeds. A good price will lead thefarmer to maintain and develop their jatropha plantation.Key words : Jatropha curcas L., land and climate suitability, financialfeasibilit
PENGARUH AMPAS SAGU DAN KOMPOS TERHADAP PRODUKTIVITAS LADA PERDU
Get PDFABSTRAKAmpas sagu berpotensi sebagai sumber bahan organik untukmeningkatkan kesuburan tanah. Pengaruh ampas sagu terhadap kesuburantanah ditentukan oleh tingkat dekomposisi dan komposisinya. Penelitianini bertujuan untuk mengetahui tingkat dekomposisi ampas sagu terhadappertumbuhan dan produksi lada perdu. Penelitian dilaksanakan di KebunPercobaan Institut Pertanian Bogor dan Balai Penelitian Tanaman Rempahdan Obat pada bulan Mei 2003 sampai April 2004. Tanaman yangdigunakan adalah tanaman lada perdu umur 4 tahun yang ditanam dibawah tanaman karet. Percobaan menggunakan rancangan acak kelompok(RAK), dengan 3 ulangan dan 6 tan/perlakuan yang menguji perlakuankombinasi antara tingkat dekomposisi ampas sagu(W) dan komposisinyadengan kompos (A), terdiri dari : W 0 = ampas sagu dekomposisi 0 bulan,W 1 = ampas sagu dekomposisi 1 bulan, dan W 2 = ampas sagu dekomposisi2 bulan dan A 1 = 100% ampas sagu, A 2 = 75% ampas sagu + 25% kompos,A 3 = 50% ampas sagu + 50% kompos dan A 4 = 25% ampas sagu + 75%kompos. Hasil penelitian menunjukkan bahwa penggunaan mulsa ampassagu 100 % dalam bentuk segar dekomposisi 1 bulan ternyata menghambatpertambahan jumlah cabang tersier lada perdu hingga akhir penelitian.Dibandingkan dengan kontrol terjadi perbedaan pengaruh yang nyataterhadap komponen produksi, sebagai respon terhadap kombinasiperlakuanW 2 A 2 , W 2 A 1, W 1 A 3 pada panjang tandan (9,13; 9,03; 8,70 cm),dan W 2 A 2 , W 2 A 1, W 0 A 4 pada jumlah biji/tandan (46,67; 43,00; 41,73biji/tandan), serta W 2 A 2 , W 2 A 1, W 2 A 3 pada bobot kering buahlada/tanaman (323,20; 314,90; 297,85 g/tanaman). Pemberian ampas sagu75% + 25% kompos (W 2 A 2 ) dan 100% (W 2 A 1 ) dekomposisi 2 bulanmampu meningkatkan jumlah biji 91 - 107% dan menghasilkan bobotkering buah yang tinggi sebesar 323,20 dan 314,90 g per tanaman.Kata kunci: Ampas sagu, mulsa, lada perdu, produktivitasABSTRACTUse of Sago Waste and Compost to Increase theProductivity of Bushy Black PepperAs a source of organic matter to improve soil fertility, sago wastecan also be used as an ameliorant and natural herbicide. The effect of sagoand compost on soil fertility is determined by the grade of decompositionand its compositions. An experiment was conducted at the experimentalgarden of Bogor Agricultural University Bogor and Indonesian Medicinaland Aromatic Crops Research Institute, from May 2003 to April 2004.Plant material used was bushy black pepper of the Petaling variety, 4 yearsold, planted under rubber trees. The treatments used were A 1 W 0 = 100%sago waste without decomposition; A 1 W 1 = 100% sago waste after 1month decomposition; A 1 W 2 = 100% sago waste after 2 monthsdecomposition; A 2 W 0 = 75% sago waste + 25% compost, withoutdecomposition; A 2 W 1 = 75% sago waste + 25% compost, after 1 monthdecomposition; A 2 W 2 = 75% sago waste + 25% compost, after 2 monthsdecomposition; A 3 W 0 = 50% sago waste + 50% compost, withoutdecomposition; A 3 W 1 = 50% sago waste + 50% compost, after 1 monthdecomposition; A 3 W 2 = 50% sago waste + 50% compost, after 2 monthsdecomposition; A 4 W 0 = 25% sago waste + 75% compost, withoutdecomposition; A 4 W 1 = 25% sago waste + 75% compost, after 1 monthdecomposition; A 4 W 2 = 25% sago waste + 75% compost, after 2 monthsdecomposition; TBO = without organic matter (control). The experimentwas performed with a randomized block design, with 3 replicates and 6plants/plot. The results showed, that 100% sago palm waste after 1 monthdecomposition hampered number of tertiary branch until the end ofresearch. The real difference on the productivity components as a respondof combination sago waste treatments wereW 2 A 2 , W 2 A 1, W 1 A 3 for lengthof stem (9.13; 9.03; 8.70 cm), and W 2 A 2 , W 2 A 1, W 0 A 4 for number ofberries/spike (46.67; 43.00; 41.73 kernels/spike), and W 2 A 2 , W 2 A 1, W 2 A 3for dry weight of berries/plant (323.20; 314.90; 297.85 g/plant).Extension of 75% sago palm waste+ 25% compost (W 2 A 2 ) and 100%(W 2 A 1 ) after 2 months decomposition were able to increase amount ofseed 91 - 107 % and dry weight of berries /plant which were323.20 and314.90 g per plant.Key words: Sago waste, mulch, bushy pepper black, productivit
VARIASI KETAHANAN GENOTIPE KENAF (Hibiscus cannabinus L.) TERHADAP NEMATODA PURU AKAR (Meloidogyne incognita)
Get PDFABSTRAKNematoda puru akar (Meloidogyne sp.) merupakan penyakit yangtergolong penting dan banyak menyerang pertanaman kenaf di lahanpengembangan maupun pembenihan, sehingga banyak menimbulkankerugian bagi petani karena terjadi penurunan produktivitasnya. Tanamankenaf (H. cannabinus) umumnya tidak tahan nematoda, namun kerabatdekat kenaf dari jenis liar seperti H. radiatus (radiatus) diketahuimengandung gen ketahanan terhadap nematoda. Persilangan inter spesifikantara kenaf dan radiatus yang beda spesies, diharapkan akan dapatmentransfer gen ketahanan dari radiatus ke kenaf, sehingga diperolehvarietas unggul kenaf yang tahan nematoda. Tujuan penelitian ini adalahuntuk mengetahui variabilitas genetik sifat ketahanan tanaman kenafterhadap serangan M. incognita pada F1 dibandingkan dengan keduatetuanya. Kegiatan persilangan interspesifik antara kenaf (H. cannabinus)dan radiatus (H. radiatus) dilakukan di KP. Karangploso Malang padaTahun 2002, sedangkan uji ketahanan nematoda puru akar (M. incognita)terhadap keturunan dan kedua tetuanya dilaksanakan di Desa Kendalrejo,Kabupaten Blitar pada bulan Februari s/d Agustus 2003. Pengujianketahanan di lapang dilakukan menggunakan metode baris tanpa ulanganpada jarak tanam 20 x 20 cm, dengan perlakuan terdiri dari lima set hasilpersilangan dan kedua tetuanya yakni 20 populasi F1, 20 populasi P1 dan20 populasi P2, dengan masing-masing populasi 20 tanaman. Hasil ujitanah di laboratorium menunjukkan bahwa rata-rata kandungan larva M.incognita (sebagai populasi awal) adalah sebesar 96 ekor/100 ml tanahdan dikategorikan sangat tinggi. Sedangkan hasil identifikasi sidik pantat(berdasarkan perenial patternnya) terhadap larva betina dewasa diketahuibahwa jenis nematoda di lokasi penelitian adalah dari spesies Meloidogyneincognita. Pengamatan dan perhitungan larva M. incognita dilakukan diLaboratarium Hama dan Penyakit Balittas, Malang. Pengamatan dilakukanterhadap jumlah puru akar per tanaman, kerusakan akar tanaman, danpopulasi larva M. incognita dalam tanah sebagai faktor R (R = reproduksilarva). Untuk menggolong-golongkan tingkat ketahanan terhadap M.incognita digunakan metode Zeck melalui indeks kerusakan akar. Darihasil penelitian menunjukkan bahwa, semua keturunan F1 dari 5persilangan interspesifik antara KR 6 x Kal II, KR 11 x Kal II, KR 12 xKal II, Hc G-1 x Kal II, dan Hc G-51 x Kal II tidak ada yang tahanterhadap serangan M. incognita. Nilai ketahanan genotipe F1 terletak diantara tetua jantan (Kal II/ radiatus) yang tahan dan tetua betina (kenaf)yang sangat rentan.Kata kunci : Hibiscus cannabinus L., interspesifik, variabilitas genetik,ketahanan, Meloidogyne incognitaABSTRACTResistance variability of kenaf (Hibiscus cannabinus L.)genotypes to root-knot nematode (Meloidogyne incognitaL.)Root Knot Nematode (Meloidogyne incognita) is an importantdisease on kenaf plantation in the development area and its nursery. Thiscondition generates reduce of productivity and loss of farmers’ income.Kenaf plants are generally not resistant to nematode infestation, so theyneed resistant gene from other species (H. radiatus) to be transfered to H.cannabinus by interspecific hybridization. The objective of this researchwas to obtain genetic variability of kenaf resistance to Meloidogyneincognita attact. Interspecific hybridization between H. cannabinus and H.radiatus was conducted at KP Karangploso, Malang in 2002. Meanwhile,evaluation of F1 lines and their parents to M. incognita resistance wasconducted at Kendalrejo, Blitar on February to August 2003. In this area,the type of soil is medium fertile-light-sandy soil containing high densitiesof root-knot nematode larvae. Five sets of F1 resulted from hybridizationbetween H. canabinus and H. radiatus and their parents were planted in arow method without replication. Subsequently, these seeds were used asthe materials in this study. The observation and evaluation of these larvaewas performed at the Phytopathology Laboratory of the IndonesianTobacco and Fiber Crops Research Institute, Malang. The preliminaryresult showed that the average of the initial population was 96 larvae per100 ml of soil, which is categorized as a very high larvae content.Furthermore, the identification to the adult female larvae (perennial patternmethod) showed that the root-knot nematode found in the location ischaracterized as M. incognita. Parameters observed were total number ofgall per plant, degree of root damage, and populations of M. incognitalarvae in the soil as a R-factor (R = larvae reproduction). The degree ofresistance to M. incognita are analyzed according to Zeck method usingroot damage index. Results of this research are : all F1 from fiveinterspesific hybridization between KR 6 x Kal II, KR 11 x Kal II, KR 12x Kal II, Hc G-1 x Kal II, and Hc G-51 x Kal II are still more sucseptiblecompared to their male parent (Kal II) which is resistant to root-knotnematode.Key words : Hibiscus cannabinus L., interspecific, genetic variability,resistance, Meloidogyne incognit