Majalah Kedokteran Andalas
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Pengaruh Pemberian Mesenchymal Stem Cell Wharton's Jelly Terhadap Aktivitas Enzim Katalase pada Tikus Wistar Model Hiperglikemia
Full text linkObjective: To determine the effect of Wharton's Jelly (MSC-WJ) mesenchymal stem cells on the activity of the catalase enzyme in hyperglycemic Wistar rats. Methods: This study used 21 male Wistar rats aged 2-3 months with a body weight of 200-300 grams according to the inclusion and exclusion criteria. Mice were divided into 3 groups: negative control (K-), positive control (K+), and treatment (P). Group K- was only given food and drink without being given treatment. The K+ group was injected intraperitoneally with alloxan at a dose of 100 mg/kg BW so that the rats became hyperglycemic. Group P was given an alloxan injection and left for a week, then given MSC-WJ 3x106 cells through the tail vein and left for four weeks. After four weeks, all groups had their blood taken to examine the catalase enzyme. Result: Mean of catalase activity enzyme in the K-, K+, and P groups were 4.22 ± 0.27 units/mg, 2.39 ± 0.19 units/mg, and 3.93 ± 0.45 units /mg, respectively. Conclusion: This shows that the administration of mesenchymal stem cells Wharton's Jelly (MSC-WJ) has an effect on increasing the serum catalase enzyme activity of Wistar rats with hyperglycemia models
Perception of online learning with anxiety and stress level among medical students
Full text linkObjective: This study aimed to find out the association between the perception of online learning and anxiety as well as stress levels among medical students. Methods: This is an observational analytic study with a cross-sectional approach. The sample was comprised of 213 undergraduate medical studentsept at Medical Faculty Universitas Sebelas Maret (UNS) and data were collected using total sampling. The study was conducted using the Taylor Manifest Anxiety Scale (TMAS), Perceived Stress Scale (PSS), and perception of online learning questionnaires. Data were analyzed using Chi-square, Cramer’s V, and Kendall tau b test. Results: This study shows a significant correlation between the perception of online learning and anxiety (p=0.049) as well as stress level (p=0.007). Sex also has an association with anxiety (p=0.010) and stress level (p=0.004). Furthermore, female has higher anxiety and stress levels compared to male. Conclusions: Perception of online learning is related to the anxiety and stress level among medical students
Aktivitas Antijamur Infusa Daun Dandang Gendis (Clinacanthus nutans (Burm.f.) Lindau) Terhadap Pertumbuhan Candida albicans
Full text linkTujuan: Mengetahui aktivitas antijamur infusa daun dandang gendis terhadap pertumbuhan Candida albicans dan mengetahui konsentrasi efektif infusa daun dandang gendis pada pertumbuhan Candida albicans;Â Metode: Skrining fitokimia infusa daun dandang gendis dilakukan dengan pengujian secara kualitatif. Pembuatan infusa daun dandang gendis dilakukan dengan merebus daun dandang gendis selama 15 menit dalam akuades yang telah dipanaskan dengan suhu 90oC. Pengujian aktivitas antijamur menggunakan metode difusi cakram dengan konsentrasi 20%, 40%, 60%, 80%, dan 100%. Flukonazol 25 ug/cakram dan Itrakonazol 8ug/cakram digunakan sebagai kontrol positif dan akuades steril digunakan sebagai kontrol negatif;Â Hasil: Berdasarkan hasil uji metabolit, didapatkan kandungan metabolit sekunder infusa daun dandang gendis yaitu fenol, saponin, tanin, terpenoid, alkaloid, dan flavonoid. Metabolit sekunder dominan pada infusa daun dandang gendis adalah fenol (+++). Pengujian infusa daun dandang gendis tidak menunjukkan adanya zona hambat meskipun diujikan pada konsentrasi 100%;Â Kesimpulan: Infusa daun dandang gendis tidak memiliki aktivitas antijamur terhadap Candida albicans
Perbedaan Rerata Absolute Lymphocyte Count Pada Pasien Coronavirus Disease 2019 Derajat Ringan-Sedang Dan Berat-Kritis
Full text linkLatar Belakang: Coronavirus disease 2019 adalah penyakit menular yang disebabkan SARS-CoV-2. Mayoritas pasien COVID-19 memiliki gejala ringan, proses inflamasi luas dapat menyebabkan perburukan klinis. Kelainan hematologi yang paling sering ditemukan pada pasien COVID-19 adalah limfopenia. Penurunan absolute lymphocyte count ditemukan pada pasien COVID-19 dengan klinis berat; Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan rerata ALC pada pasien COVID-19 derajat ringan-sedang dan berat-kritis; Metode: Penelitian analitik dengan rancangan potong lintang dilakukan terhadap 100 pasien COVID-19 derajat ringan-sedang dan berat-kritis di RSUP Dr. M. Djamil Padang sejak Juli 2022 hingga Februari 2023. Total ALC dihitung dengan rumus jumlah leukosit x % limfosit per liter darah. Data dianalisis menggunakan uji T tidak berpasangan dengan kemaknaan (p<0,05); Hasil: Subjek penelitian sebagian besar laki-laki (59%). Rerata umur 53 tahun (23-84 tahun). Rerata ALC seluruh subjek penelitian 1,48x109/L dengan nilai terendah 0,53x109/L dan tertinggi 2,95x109/L. Rerata ALC pasien COVID-19 derajat ringan-sedang 1,95x109/L dan derajat berat-kritis 1,01x109/L. Uji statistik menunjukkan ditemukan perbedaan bermakna rerata ALC pada 2 kelompok (p=0,013); Pembahasan: Rerata ALC pasien COVID-19 derajat ringan-sedang dalam rentang normal dan derajat berat-kritis di bawah rentang normal; Simpulan: Ditemukan perbedaan rerata ALC pada pasien COVID-19 derajat ringan-sedang dan berat-kritis.Â
Distribusi Mutasi Asp614Gly pada Spike Protein SARS-CoV-2 di Sumatera Barat
Full text linkObjective: This study aims to map the distribution of the Aspartate 614 to Glycine (Asp614Gly) mutation in the spike protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) circulating in West Sumatra, to understand the extent of its spread within the local population. Methods: RNA samples were taken from 98 confirmed COVID-19 cases across various area in West Sumatra. Genetic amplification and next-generation sequencing (NGS) were performed to identify the presence of the Asp614Gly mutation. The frequency of mutations was analyzed using bioinformatics software. Results: Out of the 98 samples analyzed, approximately 97% exhibited the Asp614Gly mutation. The geographic distribution of this mutation showed significant variation among the districts of West Sumatra, with the highest prevalence in urban areas. Conclusion: These findings indicate that the Asp614Gly mutation is highly prevalent in West Sumatra. Associated with increased virus transmissibility, this mutation dominates the circulating virus strains in the region, highlighting the need for ongoing genetic surveillance to guide public health interventions and vaccination strategies
Glycemic control and serum albumin in type 2 diabetes patients: What does observational study say?
No full textGlycemic control is important to prevent organ damage in Type 2 Diabetes Melitus (T2DM) Patients, especially liver and kidney. One of parameter used to see the decreasing function of both organs is serum albumin. Aim : The aim of this study was to identify the association between glycemic control and serum albumin level on T2DM patoents. Method : Â This is an observational analytical study using cross sectional design. This study was conducted at RSUP Dr. M. Djamil Padang using T2DM patients medical records on 2019-2021. The level of HA1c <7% was cathegorised as good glycemic control and Hb1c >7% was cathegorised as poor glycemic control. Results : The results showed that there was an association between glycemic control and serum albumin level on T2DM patients at RSUP Dr. M. Djamil Padang. Conclusion : Glycemic control was inversely associated with serum albumin levels in patients with T2DM in RSUP Dr. M. Djamil Padan
THE EFFECTIVENESS OF THE HEALTHY KITCHEN PROGRAM TO OVERCOME STUNTING IN IMPROVING THE NUTRITIONAL STATUS OF STUNTING TODDLERS
No full textStunting is a problem of chronic malnutrition which is caused by a lack of nutritional intake for a long time. One of the programs initiated by the BKKBN to reduce stunting rates is the Healthy Kitchen to Overcome Stunting (DASHAT) program. One of the DASHAT programs is providing local supplementary food to stunted toddlers. The aim of this research was to determine the effectiveness of DASHAT on weight gain and nutritional status of stunted toddlers during the program and after 3 months of completion of the program. This research uses a Quasy Experiment approach. The population in this study were all stunted toddlers who received the DASHAT program, while the sample in this study was 34 toddlers taken by random sampling in three areas of the South Padang District Health Center, Padang City. The results of this research show that the DASHAT program is effective in increasing the weight of toddlers with a p-value of 0.000 during the program and 0.048 after the program, but is not effective in changing nutritional status (TB/U) after the DASHAT program ends. Maximum monitoring is needed from various parties regarding stunting toddlers after the DASHAT program is completed
E-Cigarette or Vaping Use-Associated Lung Injury
Full text linkPendahuluan: Rokok elektrik pertama kali diperkenalkan di Cina pada tahun 2003, kemudian dibawa ke Eropa tahun 2005 dan Amerika tahun 2007. Rokok elektrik adalah perangkat yang memberikan pengalaman merokok dengan mengirimkan nikotin atau kandungan zat tertentu, ke dalam paru melalui penguapan. Rokok elektrik umumnya mengandung propilen glikol, gliserol, cairan perasa, logam berat, nikotin, dan senyawa organik volatil (VOC). Penggunaan rokok elektrik setiap hari di Amerika mencapai 7,6% dan 27,5% penggunaan dalam 30 hari terakhir.Kasus cedera paru akibat penggunaan produk rokok elektrik dikenal dengan istilah e-cigarette or vaping use-associated lung injury (EVALI) dilaporkan pertama kali pada 1 Agustus 2019. Sebanyak lebih dari 2.800 kasus rawat inap dan 68 kematian akibat EVALI per Maret 2020. Lebih dari 50% pasien meninggal memiliki diagnosis sindrom gangguan pernapasan akut.Â
Pengaruh pemberian salep ekstrak etanol daun sirih terhadap jumlah fibroblas penyembuhan luka Rattus novergicus
Full text linkLuka merupakan suatu keadaan hilang atau rusaknya sebagian dari jaringan tubuh. Fibroblas menjadi agen utama dalam proses penyembuhan luka. Daun sirih hijau memiliki kandungan tanin, saponin, dan flavonoid yang berfungsi sebagai antimikroba dan antiseptik sehingga dianggap dapat meningkatkan fibroblas. Tujuan: Mengetahui efek salep ekstrak etanol 70% daun sirih terhadap jumlah fibroblas pada penyembuhan luka tikus putih jantan galur Wistar. Metode: Penelitian menggunakan 30 tikus yang dibagi menjadi 2 kelompok yang terdiri pengamatan hari ke-7 dan hari ke-14. Setiap kelompok pengamatan terdiri 5 kelompok, kelompok kontrol Negatif (KN), kelompok kontrol Positif (KP), kelompok Sirih 7,5% (SI), kelompok Sirih 15% (SII), kelompok Sirih 30% (SIII). Analisis data rerata jumlah fibroblas pada tiap kelompok perlakuan pada hari ke-7 menggunakan uji Shapiro-Wilk dan dilajutkan uji Kruskal-Wallis. Uji one way ANOVA dan dilanjutkan uji Pos Hoc LSD untuk melihat hasil perbandingan rerata jumlah fibroblas pada hari ke 14 untuk mengetahui perbedaan antar kelompok perlakuan. Hasil: Rerata jumlah fibroblas antar kelompok perlakuan ekstrak daun sirih lebih kecil dibandingkan dengan kedua kelompok kontrol pada hari ke-7 dan hari ke-14. Kesimpulan: Salep ekstrak etanol 70% daun sirih dosis 7,5%, 15% dan 30% tidak efektif meningkatkan jumlah fibroblas pada hari ke-7 dan hari ke-14
Hubungan Healthy Eating Index Dengan Kenaikan Berat Badan Ibu Selama Kehamilan Di Sumatera Barat
Full text linkAbstractObjective: To determine the association between the healthy eating index of pregnant women and maternal weight gain during pregnancy in West Sumatra.Methods: Research with a cross-sectional design in West Sumatra on 110 pregnant women.This research is an analysis of secondary data from the study "Giving Dadiah on pregnancy and birth outcomes in West Sumatra".The study used data from 24-hour recall of pregnant women in the first trimester, second trimester and third trimester and pregnant women's weight data.Results: The study showed that there was a relationship between the HEI of pregnant women and the increase in maternal weight during pregnancy with p = 0.049 with a value of r = 0.188.Conclusion: There is a relationship between the healthy eating index of pregnant women and the increase in maternal weight during pregnancy.Keywords: Â healthy eating index; weight gain; pregnant woma