Jurnal Teknologi Laboratorium
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Isolasi Candida albicans Dari Swab Mukosa Mulut Penderita Diabetes Melitus Tipe 2
No full textCandida albicans can be pathogen when immunity had decreased and physiological function is impaired, such as in diabetes mellitus type 2. This study aims to isolation Candida albicans that collected from the oral cavity of diabetes mellitus type 2 patients. This research is conducted with a descriptive study by observing the presence of Candida albicans in 30 samples of diabetes mellitus type 2 patients, which grows on Potato Dextrose Agar. The microscopic observation by LPCB staining of yeasts, blastospores, pseudohyphae, chlamydospores and germ tubes in human serum suspension that incubated at 370C for 2-3 hours. Based on this research was found 14 (46,7%) patients from 30 patients were positive Candida albicans.Candida pathogen when immunity had decreased and changes in physiological functions, such as in diabetes mellitus. This study aims to isolation Candida albicans that collected from oral cavity of diabetic type 2 patients. This descriptive study was determined by observing the presence of Candida albicans in 30 samples of diabetic patients, which grows on Potato Dextrose Agar, then the microscopic observation by LPCB staining of yeasts, blastospores, pseudohyphae, chlamydospores and germ tubes in human serum suspension that incubated at 370C for 2-3 hours, from 30 DM patients studied, it was found positive result of Candida albicans fungus on mucosa mouth swab patient type 2 diabetes, that is 14 people with percentage 46,7%
Imunomodulator Ekstrak Etanol Daun Mimba (Azadirachta indica) terhadap Jumlah Sel Makrofag Peritoneal pada Mencit yang Diinduksi Vaksin BCG
No full textThis research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients
Variasi Konsentrasi Alfa Siklodekstrin dan Waktu Sentrifugasi Dalam Preparasi Serum Lipemik Pada Pemeriksaan Glukosa Metode GOD-PAP
No full textExamination of glucose levels of the GOD-PAP method in serum may be disrupted by the presence of turbidity caused by lipemic serum, thus causing high false serum glucose levels to result. The addition of alpha-cyclodextrin (α-CD) may bind the lipemic in serum. This study aims to find out how the concentration and time of optimal centrifugation with the addition of alpha-cyclodextrin. Added variation alpha-cyclodextrin concentration 0.5%, 1%, and 1.5% in pooled serum with variation concentration of triglycerides ± 1000 mg/dL, ± 1500 mg/dL and ± 2000 mg/dL incubated for 30 minute at 4°C. Centrifuged at 3000 rpm with variation of centrifugation time for 5 minutes, 10 minutes and 15 minutes at 4°C to form precipitate and supernatant. Then the supernatant measured the glucose GOD-PAP and triglycerides GPO-PAP using a photometer. The result were no significant differences in variations centrifugation time and variations alpha-cyclodextrin concentrations after comparison with serum glucose pooled levels (base line) in the ANOVA test, Kruskall Wallis Test and Mann Whitney test. There was no significant difference with pooled serum (base line) at the time of triglyceride concentration ± 1000 mg/dL with alpha-cyclodextrin concentration 0.5% and centrifugation time at 10 minute, triglyceride concentration ± 1500 mg/dL and ± 2000 mg/dL with alpha-cyclodextrin concentration 1%, centrifugation time at 5 minute.Examination of glucose levels of the GOD-PAP method in serum may be disrupted by the presence of turbidity caused by lipemic serum, thus causing high false serum glucose levels to result. The addition of alpha-cyclodextrin (α-CD) may bind the lipemic in serum. This study aims to find out how the concentration and time of optimal centrifugation with the addition of alpha-cyclodextrin. Added variation alpha-cyclodextrin concentration 0.5%, 1%, and 1.5% in pooled serum with variation concentration of triglycerides ± 1000 mg/dL, ± 1500 mg/dL and ± 2000 mg/dL incubated for 30 minute at 4°C. Centrifuged at 3000 rpm with variation of centrifugation time for 5 minutes, 10 minutes and 15 minutes at 4°C to form precipitate and supernatant. Then the supernatant measured the glucose GOD-PAP and triglycerides GPO-PAP using a photometer. The result were no significant differences in variations centrifugation time and variations alpha-cyclodextrin concentrations after comparison with serum glucose pooled levels (base line) in the ANOVA test, Kruskall Wallis Test and Mann Whitney test. There was no significant difference with pooled serum (base line) at the time of triglyceride concentration ± 1000 mg/dL with alpha-cyclodextrin concentration 0.5% and centrifugation time at 10 minute, triglyceride concentration ± 1500 mg/dL and ± 2000 mg/dL with alpha-cyclodextrin concentration 1%, centrifugation time at 5 minute
Efektivitas Variasi Garam Salmiak (NH4Cl) Dan Sentrifugasi Pada Pemeriksaan Basil Tahan Asam Penderita Tuberculosis
No full textAccording to a report by WHO Indonesia, it is a case number three infected with tuberculosis. Tuberculosis disease is caused by Mycobacterium tuberculosis that belongs to acid-fast bacilli bacteria (BTA). Sputum smear examination microscopically still has many deficiencies caused by mucoprotein in sputum so that BTA cannot be separated from sputum, this is causing a false negative in reading result Microscope. The objective of this research is to know the most effective variation of concentration, speed and time of centrifugation on smear examination in tuberculosis patients. The research method used is experimental method and Design of complete Random Block design. Data were analyzed by statistic test with ANOVA test on SPSS. The result of the research is that at concentration 2% NH4Cl It can increase the number of BTA with optimal centrifugation speed / effective at 5000 rpm for 15 minutes. Suggestion for further research is to use salmaniac salt concentration with a concentration lower than 2%
Kajian Aktivitas Antikanker Ekstrak Daun Gude (cajanus cajan) Terhadap Sel Kanker Kolon Secara in Vitro
No full textColorectal cancer is one of the five type of highest insiden in Indonesia. One of development cancer therapeutics directed to combination of chemotherapeutic agent and chemopreventive compounds. One approach to finding a chemopreventive compounds is through the exploration of natural ingredient especialy herbs. Flavonoids is group of compounds from plants that have been widely studied as anti-cancer activity. Leave of pigeonpea (Cajanus cajan) is, kind of Legumes plants rich in flavonoids among others cajanol, quercetin, luteolin, apigenin formonentin, vitexin, orientin, biochanin A, pinostrobin, dan isorhamnetin. This study aims to determine the content of chemical compounds in the methanol extract of leave pigeon pea that has anticancer activity using WiDr colorectal cancer cells with MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) method, followed by flowcytometry test. The result of the cytotoxicity test of methanol extract of pigeonpea leaves have IC50 of 307 ug/ml and with flowcytometry method showed necrosis WiDr cell.Colorectal cancer is one of the five type of highest insiden in Indonesia. One of development cancer therapeutics directed to combination of chemotherapeutic agent and chemopreventive compounds. One approach to finding a chemopreventive compounds is through the exploration of natural ingredient especialy herbs. Flavonoids is group of compounds from plants that have been widely studied as anti-cancer activity. Leave of pigeonpea (Cajanus cajan) is, kind of Legumes plants rich in flavonoids among others cajanol, quercetin, luteolin, apigenin formonentin, vitexin, orientin, biochanin A, pinostrobin, dan isorhamnetin. This study aims to determine the content of chemical compounds in the methanol extract of leave pigeon pea that has anticancer activity using WiDr colorectal cancer cells with MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) method, followed by flowcytometry test. The result of the cytotoxicity test of methanol extract of pigeonpea leaves have IC50 of 307 ug/ml and with flowcytometry method showed necrosis WiDr cell
Gambaran Sedimen Urine Pada Masyarakat Yang Mengkonsumsi Air Pegunungan Di Kecamatan Kendari Barat Kota Kendari
No full textAir yang bersih dan sehat merupakan kualifikasi yang sangat diperlukan untuk pemenuhan kebutuhan manusia. Air harus mempunyai persyaratan khusus agar air tersebut tidak menimbulkan penyakit bagi manusia. Air pegunungan yang dikonsumsi masyarakat Kelurahan Sodoha Kota Kendari, pada umumnya memiliki kualitas yang baik, tetapi dapat berubah kualitasnya karena berbagai faktor. Penelitian ini bertujuan untuk mengetahui gambaran sedimen urin pada masyarakat yang mengkonsumsi air pegunungan di Kecamatan Kendari Barat Kota Kendari.Jenis penelitian adalah deskriptif, dengan menentukan prosentase sedimen urin masyarakat yang mengkonsumsi air pegunungan melalui pemeriksaan laboratorium. Jumlah sampel dalam penelitian ini adalah 30 Kepala Keluarga yang tinggal di Kelurahan Sodohoa Kecamatan Kendari Barat Kota Kendari, dengan teknik pengambilan simple random sampling (sampel acak secara sederhana). Berdasarkan hasil penelitian, ditemukan prosentase sedimen urin abnormal, yaitu leuokosit dan eritrosit 6,7 %, epitel 23,3 %, silinder, kalsium oksalat, asam urat, dan bakteri masing-masing 3, 3 %. Kesimpulan dari penelitian menunjukkan adanya sedimen organik yang meliputi, leukosit, eritrosit, silinder, epitel dan bakteri pada masyarakat yang mengkonsumsi air pegunungan Kelurahan Sodohoa Kecamatan Kendari Barat Kota Kendari.Air yang bersih dan sehat merupakan kualifikasi yang sangat diperlukan untuk pemenuhan kebutuhan manusia. Air harus mempunyai persyaratan khusus agar air tersebut tidak menimbulkan penyakit bagi manusia. Air pegunungan yang dikonsumsi masyarakat Kelurahan Sodoha Kota Kendari, pada umumnya memiliki kualitas yang baik, tetapi dapat berubah kualitasnya karena berbagai faktor. Penelitian ini bertujuan untuk mengetahui gambaran sedimen urin pada masyarakat yang mengkonsumsi air pegunungan di Kecamatan Kendari Barat Kota Kendari.Jenis penelitian adalah deskriptif, dengan menentukan prosentase sedimen urin masyarakat yang mengkonsumsi air pegunungan melalui pemeriksaan laboratorium. Jumlah sampel dalam penelitian ini adalah 30 Kepala Keluarga yang tinggal di Kelurahan Sodohoa Kecamatan Kendari Barat Kota Kendari, dengan teknik pengambilan simple random sampling (sampel acak secara sederhana). Berdasarkan hasil penelitian, ditemukan prosentase sedimen urin abnormal, yaitu leuokosit dan eritrosit 6,7 %, epitel 23,3 %, silinder, kalsium oksalat, asam urat, dan bakteri masing-masing 3, 3 %. Kesimpulan dari penelitian menunjukkan adanya sedimen organik yang meliputi, leukosit, eritrosit, silinder, epitel dan bakteri pada masyarakat yang mengkonsumsi air pegunungan Kelurahan Sodohoa Kecamatan Kendari Barat Kota Kendari
Uji Antibakteri Ekstrak Metanol Kulit Buah Manggis (garcinia mangostana l.) Terhadap Staphyllococcus aureus dan Escherechia coli
No full textAlfa mangostin, gamma-mangostin and xanthone group compounds are secondary metabolites contained in mangosteen (G. mangostana L.) which can be isolated from the fruit, bark, leaves and rind of mangosteen. All three compounds were shown to inhibit stronger against Mycobacterium tuberculosis. Alfa mangostin also active against Staphylococcus aureus and Enterococcus bacteria that are resistant to vancomisin and methicillin. Objective of this study to determine the antibacterial activity of methanol extract of mangosteen rind against Staphyllococcus aureus and Escherechia coli in vitro.
Antibacterial activity test used is the diffusion wells and paper disc diffusion method. The test results obtained antibacterial activity of methanol extract of mangosteen rind can inhibit the growth of Staphyllococcus aureus at a concentration of 16%, but below its resistance zone diameter Cyprofloxacin. As against Escherichia coli, the methanol extract of mangosteen rind could not inhibit the growth of bacteria
Optimasi Air Perasan Buah Merah (Pandanus sp.) Pada Pemeriksaan Telur Cacing
No full textWorm infections or can be called by worming included into an infection caused by a parasite. Soil Transmitted Helminths Intestinal Nematodes are worms groups are in their life cycle to reach the infective stage requires soil with certain conditions. Eosin 2% is the dye that is used in the examination of Intestinal Nematode worm eggs. Red fruit (Pandanus sp.) which is a natural plant material and acidic contain carotenoids which produces orange-red pigment. Beta carotene is the predominant pigment of red-orange color that is found naturally in plants and fruits. The aim of this research is to determine the best concentration from variation of red fruit (Pandanus sp.) juice that optimally to color the eggs of the worm. Research conducted experiments with various concentration ratio of red fruit (Pandanus sp.) juice and distilled water (1, 1:1, 1:2, 1:3, 1:4, 1:5). From this research it found that the results indicate the ratio of concentrations of red fruit (Pandanus sp.) juice and distilled water (1:2) can be used as an alternative reagent eosin 2% for their examination of worm eggs. But in the visual field that uses red fruit (Pandanus sp.) juice and distilled water (1:2) still looks much dirt as a nuisance and does not give a contrasting background. It can conclude that red fruit (Pandanus sp.) juice can use to color the eggs of the worm.Worm infections or can be called by worming included into an infection caused by a parasite. Soil Transmitted Helminths Intestinal Nematodes are worms groups are in their life cycle to reach the infective stage requires soil with certain conditions. Eosin 2% is the dye that is used in the examination of Intestinal Nematode worm eggs. Red fruit (Pandanus sp.) which is a natural plant material and acidic contain carotenoids which produces orange-red pigment. Beta carotene is the predominant pigment of red-orange color that is found naturally in plants and fruits. The aim of this research is to determine the best concentration from variation of red fruit (Pandanus sp.) juice that optimally to color the eggs of the worm. Research conducted experiments with various concentration ratio of red fruit (Pandanus sp.) juice and distilled water (1, 1:1, 1:2, 1:3, 1:4, 1:5). From this research it found that the results indicate the ratio of concentrations of red fruit (Pandanus sp.) juice and distilled water (1:2) can be used as an alternative reagent eosin 2% for their examination of worm eggs. But in the visual field that uses red fruit (Pandanus sp.) juice and distilled water (1:2) still looks much dirt as a nuisance and does not give a contrasting background. It can conclude that red fruit (Pandanus sp.) juice can use to color the eggs of the worm
Potensi Enzim Bromelin Sari Buah Nanas (ananas comosus l.) Dalam Meningkatkan Kadar Protein Pada Tahu
No full textTofu is one of processed soybeans that have a high nutritional value and low price. Levels of protein in tofu can be enhanced by enzymatically. One of the protease enzyme which is commonly used in the food industry is bromelain enzyme. The bromelain enzyme in pineapple is widely available. The purpose of this study was to determine the effect of the bromelain enzyme from pineapple juice to protein content by examining the enzyme concentration and incubation time to get the best protein content.This research was quation experiment. The experimental design for this research was randomized block design with 2 factors. Factor I consist of 4 levels (enzyme concentration 35%, 40%, 45% and 50%) and factor II consist of 2 levels (incubation period with enzyme of 6 and 12 hours). The data obtained were then analyzed statistically by multiple linear regression test.The results showed that the best protein content was obtained from the preparation of tofu with the addition of bromelin enzyme from pineapple juice with concentration of 50% and with the incubation time for 12 hours, that is 16,6195%. Statistical test results obtained value of p <0.05, so it can be concluded that there were a relationship/correlation between the enzyme concentration and incubation time to the protein content. Based on the results of research can be suggested that bromelin enzyme from pineapple juice can be used to increase protein content in tofu.Tofu is one of processed soybeans that have a high nutritional value and low price. Levels of protein in tofu can be enhanced by enzymatically. One of the protease enzyme which is commonly used in the food industry is bromelain enzyme. The bromelain enzyme in pineapple is widely available. The purpose of this study was to determine the effect of the bromelain enzyme from pineapple juice to protein content by examining the enzyme concentration and incubation time to get the best protein content.This research was quation experiment. The experimental design for this research was randomized block design with 2 factors. Factor I consist of 4 levels (enzyme concentration 35%, 40%, 45% and 50%) and factor II consist of 2 levels (incubation period with enzyme of 6 and 12 hours). The data obtained were then analyzed statistically by multiple linear regression test.The results showed that the best protein content was obtained from the preparation of tofu with the addition of bromelin enzyme from pineapple juice with concentration of 50% and with the incubation time for 12 hours, that is 16,6195%. Statistical test results obtained value of p <0.05, so it can be concluded that there were a relationship/correlation between the enzyme concentration and incubation time to the protein content. Based on the results of research can be suggested that bromelin enzyme from pineapple juice can be used to increase protein content in tofu
Daya Antifungi Infusa Daun Kenikir ( cosmos caudatus k. ) Terhadap Pertumbuhan Jamur Candida albicans Secara in Vitro
No full textDaun kenikir dapat digunakan sebagai obat tradisional, salah satunya sebagai obat penyakit yang disebabkan oleh infeksi bakteri Staphylococcus aureus, belum ada laporan tentang penggunaan atau penelitian daun kenikir untuk pengobatan penyakit yang disebabkan jamur Candida albicans. Candida albicans merupakan jamur oportunistik yang dapat menyebabkan kandidiasis jika terjadi faktor predisposisi. Penelitian ini bertujuan untuk mengetahui daya antifungi infusa daun kenikir terhadap pertumbuhan jamur Candida albicans secara in vitro. Infusa daun kenikir diperoleh dari proses infudasi dengan menggunakan air sebagai larutan pencair. Infusa daun kenikir dibuat dalam berbagai konsentrasi (60%, 65%, 70%, 75%, 80%, 85%, 90%). Setiap konsentrasi diuji daya hambat jamur dengan metode sumuran. Data berupa diameter zona hambat dengan satuan millimeter (mm) dan dilakukan 4X pengulangan tiap konsentrasi. Hasil uji daya hambat infusa duan kenikir terhadap pertumbuhan jamur Candida albicans konsentrasi 60%, 65%, 70%, 75%, 80%, 85%, 90% sebesar 5,5 mm, 5,75 mm, 5,75 mm, 5,75 mm, 6,25 mm, 6,5 mm dan 6,5 mm. Daya antifungi infusa daun kenikir terhadap pertumbuhan jamur Candida albicans pada konsentrasi paling tinggi (90%) mempunyai persentase sebesar 22% dibandingkan ketokonazol.Daun kenikir dapat digunakan sebagai obat tradisional, salah satunya sebagai obat penyakit yang disebabkan oleh infeksi bakteri Staphylococcus aureus, belum ada laporan tentang penggunaan atau penelitian daun kenikir untuk pengobatan penyakit yang disebabkan jamur Candida albicans. Candida albicans merupakan jamur oportunistik yang dapat menyebabkan kandidiasis jika terjadi faktor predisposisi. Penelitian ini bertujuan untuk mengetahui daya antifungi infusa daun kenikir terhadap pertumbuhan jamur Candida albicans secara in vitro. Infusa daun kenikir diperoleh dari proses infudasi dengan menggunakan air sebagai larutan pencair. Infusa daun kenikir dibuat dalam berbagai konsentrasi (60%, 65%, 70%, 75%, 80%, 85%, 90%). Setiap konsentrasi diuji daya hambat jamur dengan metode sumuran. Data berupa diameter zona hambat dengan satuan millimeter (mm) dan dilakukan 4X pengulangan tiap konsentrasi. Hasil uji daya hambat infusa duan kenikir terhadap pertumbuhan jamur Candida albicans konsentrasi 60%, 65%, 70%, 75%, 80%, 85%, 90% sebesar 5,5 mm, 5,75 mm, 5,75 mm, 5,75 mm, 6,25 mm, 6,5 mm dan 6,5 mm. Daya antifungi infusa daun kenikir terhadap pertumbuhan jamur Candida albicans pada konsentrasi paling tinggi (90%) mempunyai persentase sebesar 22% dibandingkan ketokonazol