Jurnal Teknologi Laboratorium
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Efektivitas Berbagai Konsentrasi Infusa Daun Salam (Eugenia polyantha Wight) terhadap Daya Antibakteri Staphylococcus aureus Secara In Vitro
No full textStaphylococcus aureus is one of the acne-causing bacterias. Chemical compound in the Bay leaves are well-known as an antibacterial agent to inhibit the growth of Staphylococcus aureus.
This study aimed to determine the influence of various Bay leaf (Eugenia polyantha Wight) infusing concentration toward the inhibition of Staphylococcus aureus growth.
This research is experimental study with post-test and control group design. The sensitivity test methods toward Staphylococcus aureus were divided into five groups of bay leaf infusing concentration of 60%, 70%, 80%, 90%, and 100%. This method are used in 6 times to obtained 30 datas. Then, the datas were analyzed descriptively and analytically by ANOVA using SPSS 16.0 for Windows.
The infusion of Bay leaves had inhibition activity against Staphylococcus aureus growth based on ANOVA test which showed a significance value 0.000 <0.05.
There was a proof that the infusion of Bay leave have an effect to inhibit the growth of Staphylococcus aureus.Staphylococcus aureus is one of the acne-causing bacterias. Chemical compound in the Bay leaves are well-known as an antibacterial agent to inhibit the growth of Staphylococcus aureus.
This study aimed to determine the influence of various Bay leaf (Eugenia polyantha Wight) infusing concentration toward the inhibition of Staphylococcus aureus growth.
This research is experimental study with post-test and control group design. The sensitivity test methods toward Staphylococcus aureus were divided into five groups of bay leaf infusing concentration of 60%, 70%, 80%, 90%, and 100%. This method are used in 6 times to obtained 30 datas. Then, the datas were analyzed descriptively and analytically by ANOVA using SPSS 16.0 for Windows.
The infusion of Bay leaves had inhibition activity against Staphylococcus aureus growth based on ANOVA test which showed a significance value 0.000 <0.05.
There was a proof that the infusion of Bay leave have an effect to inhibit the growth of Staphylococcus aureus
Pengaruh Penambahan Bakteri Probiotik yang Dipacu dengan Prebiotik Ubi Jalar Terhadap Penurunan Jumlah Bakteri Shigella dysenteriae Secara In Vitro
No full textBakteri probiotik adalah mikroba yang bersifat menguntungkan dari golongan Bakteri Asam Laktat (BAL). Antara bakteri yang menguntungkan dan patogen akan terjadi kompetisi. Pertumbuhan Bakteri Asam Laktat (BAL) di usus manusia dapat distimulasi dengan cara memberikan substrat- substrat yang dapat dicerna oleh bakteri tersebut sehingga populasinya meningkat dan melawan bakteri patogen. Substrat- substrat yang dapat digunakan oleh BAL untuk menstimulasi pertumbuhannya dikenal dengan nama prebiotik. Tujuan penelitian untuk mengetahui pengaruh penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap jumlah penurunan bakteri Shigella dysenteriae. Desain penelitian ini adalah Post Test with Control. Dalam desain penelitian ini terdapat kontrol dan kelompok eksperimen. Penghitungan jumlah Bakteri Shigella dysenteriae dengan menggunakan selektif media Mac Conkey Agar. Data didapatkan dari jumlah bakteri Shigella dysenteriae setelah penambahan probiotik Lactobacillus casei. Pengolahan data menggunakan software pengolah data. Uji statistik yang digunakan adalah One Way Anova dengan taraf signifikan 5%. Hasil penelitian yang telah dilakukan didapatkan rerata jumlah penurunan bakteri Shigella dysenteriae pada media Mac Conkey Agar adalah 48.14 x 105 CFU/ml, 45.76 x 105 CFU/ml, 42.10 x 105 CFU/ml, 35.90 x 105 CFU/ml, 32.76 x 105 CFU/ml. Hasil uji Anova One Way diperoleh nilai signifikansi 0.000 (<0.05). Ada pengaruh bermakna penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap penurunan jumlah bakteri Shigella dysenteriae
PENGGUNAAN BERBAGAI KONSENTRASI EKSTRAK ETHANOL DAUN RAMBUTAN (Nephelium lappaceum L.) TERHADAP MORTALITAS LARVA Culex quinquefasciatus
No full textMosquitoes are insects which are responsible in transmission process of many diseases in humans and animals. One type that is often found is Culex quinquefasciatus. Culex quinquefasciatus mosquitoes serve as vectors of disease filariasis and chikungunya. the mosquito's prevention that uses synthetic insecticide affects the negative side for the environment. Natural insecticide is the safe way that can be used as insecticide.Rambutan’s leaves contain compounds of saponin and tanin, it be used for larvacide’s.This research aims to find out about ethanol extract of rambutan's leaves that can be used for larvacide, the impact, the influence of ethanol extract of rambutan's leaves towards larva mortality and the effective concentrate in the researchThis research is quasi experimental with Post Test Control Group Design. The concentrate extract of rambutan's leaves that is used is 0.025%, 0.05%, 0.1%, 0.2%, 0.4%,and 0.8% with duration 24 hours. Then, it is counted the amount of dead larvae. Then, the result is analyzed statically using Anova One Way and linear regression.The result showed that the percentage of the death of larva Culex quinquefasciatus in concentrate 0.025%, 0.05%, 0.1%,0.2%,0.4%, and 0.8% each of them are 77%,86%,87%,95%,98%,and 100%. The result of Anova One Way showed that the significant value is 0.000 (<0.05) which means that there is an impact of using rambutan's leaves extract towards larva mortality Culex quinquefasciatus.The use of Ethanol extract can cause the mortality of larva Culex quinquefasciatus.Mosquitoes are insects which are responsible in transmission process of many diseases in humans and animals. One type that is often found is Culex quinquefasciatus. Culex quinquefasciatus mosquitoes serve as vectors of disease filariasis and chikungunya. the mosquito's prevention that uses synthetic insecticide affects the negative side for the environment. Natural insecticide is the safe way that can be used as insecticide.Rambutan’s leaves contain compounds of saponin and tanin, it be used for larvacide’s.This research aims to find out about ethanol extract of rambutan's leaves that can be used for larvacide, the impact, the influence of ethanol extract of rambutan's leaves towards larva mortality and the effective concentrate in the researchThis research is quasi experimental with Post Test Control Group Design. The concentrate extract of rambutan's leaves that is used is 0.025%, 0.05%, 0.1%, 0.2%, 0.4%,and 0.8% with duration 24 hours. Then, it is counted the amount of dead larvae. Then, the result is analyzed statically using Anova One Way and linear regression.The result showed that the percentage of the death of larva Culex quinquefasciatus in concentrate 0.025%, 0.05%, 0.1%,0.2%,0.4%, and 0.8% each of them are 77%,86%,87%,95%,98%,and 100%. The result of Anova One Way showed that the significant value is 0.000 (<0.05) which means that there is an impact of using rambutan's leaves extract towards larva mortality Culex quinquefasciatus.The use of Ethanol extract can cause the mortality of larva Culex quinquefasciatus
Pengaruh Lama Perendaman Koro Bengu (Mucuna pruriens) Dalam Air Kapur (Ca(OH)2) Terhadap Kadar Asam Siana (HCN)
No full textBackground: Indonesian people are still focused on commodity soybeans as the main source of protein, while the use of other commodities such as koro benguk still very minimal. The lack of utilization of this koro benguk because it contains a natural form of glokusida cyanogenic compounds that can undergo enzymatic hydrolysis into hydrogen cyanide (HCN), which is toxic. Because cyanide acid very easily soluble in water, then use the soaking lime-water (Ca(OH)2) is alkaline which was quite effective in neutralizing HCN in koro benguk.Objectives: Determine the effect of soaking time in lime-water koro benguk on levels of cyanide and lime to know if the water is more effective than plain water in neutralizing HCN of koro benguk.Method: Research with post test design with control group. Object of this study was white koro benguk varieties obtained 4-6 months old in Nogosari, Purwosari, District Girimulyo, Kulon Progo, DIY is done soaking in water as a control group and lime-water 100 mg / L as the treatment group during 12, 24 and 36 hours later be distilled. Distillate is reacted with 1% picric acid and then measured the levels of HCN by spectrophotometry.Result: HCN levels koro benguk on soaking in water for 12 hours is 20,736 mg / kg, for 24 hours was 19,348 mg / kg and for 36 hours was 16,786 mg / kg. While the levels of HCN in lime-water soaking of 100 mg / L for 12 hours was 19,020 mg / kg, for 24 hours is 13,635 mg / kg and for 36 hours was 9,307 mg / kg. One way ANOVA test results in the treatment group obtained significant value 0.000 (P <0.05).Conclusion: There was a significant effect of soaking time koro benguk in lime-water on levels of cyanide. Soaking in lime-water to neutralize the cyanide acid to be more effective than soaking in water
Pengaruh Penambahan Bakteri Probiotik yang Dipacu dengan Prebiotik Ubi Jalar Terhadap Penurunan Jumlah Bakteri Shigella dysenteriae Secara In Vitro
No full textBakteri probiotik adalah mikroba yang bersifat menguntungkan dari golongan Bakteri Asam Laktat (BAL). Antara bakteri yang menguntungkan dan patogen akan terjadi kompetisi. Pertumbuhan Bakteri Asam Laktat (BAL) di usus manusia dapat distimulasi dengan cara memberikan substrat- substrat yang dapat dicerna oleh bakteri tersebut sehingga populasinya meningkat dan melawan bakteri patogen. Substrat- substrat yang dapat digunakan oleh BAL untuk menstimulasi pertumbuhannya dikenal dengan nama prebiotik.
Tujuan penelitian untuk mengetahui pengaruh penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap jumlah penurunan bakteri Shigella dysenteriae.
Desain penelitian ini adalah Post Test with Control. Dalam desain penelitian ini terdapat kontrol dan kelompok eksperimen. Penghitungan jumlah Bakteri Shigella dysenteriae dengan menggunakan selektif media Mac Conkey Agar. Data didapatkan dari jumlah bakteri Shigella dysenteriae setelah penambahan probiotik Lactobacillus casei. Pengolahan data menggunakan software pengolah data. Uji statistik yang digunakan adalah One Way Anova dengan taraf signifikan 5%.
Hasil penelitian yang telah dilakukan didapatkan rerata jumlah penurunan bakteri Shigella dysenteriae pada media Mac Conkey Agar adalah 48.14 x 105 CFU/ml, 45.76 x 105 CFU/ml, 42.10 x 105 CFU/ml, 35.90 x 105 CFU/ml, 32.76 x 105 CFU/ml. Hasil uji Anova One Way diperoleh nilai signifikansi 0.000 (<0.05).
Ada pengaruh bermakna penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap penurunan jumlah bakteri Shigella dysenteriaeBakteri probiotik adalah mikroba yang bersifat menguntungkan dari golongan Bakteri Asam Laktat (BAL). Antara bakteri yang menguntungkan dan patogen akan terjadi kompetisi. Pertumbuhan Bakteri Asam Laktat (BAL) di usus manusia dapat distimulasi dengan cara memberikan substrat- substrat yang dapat dicerna oleh bakteri tersebut sehingga populasinya meningkat dan melawan bakteri patogen. Substrat- substrat yang dapat digunakan oleh BAL untuk menstimulasi pertumbuhannya dikenal dengan nama prebiotik.
Tujuan penelitian untuk mengetahui pengaruh penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap jumlah penurunan bakteri Shigella dysenteriae.
Desain penelitian ini adalah Post Test with Control. Dalam desain penelitian ini terdapat kontrol dan kelompok eksperimen. Penghitungan jumlah Bakteri Shigella dysenteriae dengan menggunakan selektif media Mac Conkey Agar. Data didapatkan dari jumlah bakteri Shigella dysenteriae setelah penambahan probiotik Lactobacillus casei. Pengolahan data menggunakan software pengolah data. Uji statistik yang digunakan adalah One Way Anova dengan taraf signifikan 5%.
Hasil penelitian yang telah dilakukan didapatkan rerata jumlah penurunan bakteri Shigella dysenteriae pada media Mac Conkey Agar adalah 48.14 x 105 CFU/ml, 45.76 x 105 CFU/ml, 42.10 x 105 CFU/ml, 35.90 x 105 CFU/ml, 32.76 x 105 CFU/ml. Hasil uji Anova One Way diperoleh nilai signifikansi 0.000 (<0.05).
Ada pengaruh bermakna penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap penurunan jumlah bakteri Shigella dysenteria
Pengaruh Berbagai Dosis Jus Buah Sirsak (Annona muricata L.) Terhadap Penurunan Kadar Kolesterol Low Density Lipoprotein (LDL) Serum Tikus Putih (Rattus norvegicus) Dislipidemia
No full textConsumption of excessive fat in the food that we consume daily can increase cholesterol, LDL cholesterol and triglycerides in the blood that is called dyslipidemia. Diet, exercise and drugs are the effort can be done to lower the lipid levels. The herbs have been widely used as an alternative to lower cholesterol levels because the active substances contained antihiperlipid properties. Soursop has many benefits in curing some diseases. Soursop contains an active ingredient that can lower LDL’s cholesterol levels, including vitamin C, fiber and niacin. Therefore, the researchers wanted to do experiment using soursop as an alternative for lowering LDL’s cholesterol levels in dyslipidemia white rat serum.The purpose of this experiment was to determine the effect of various doses of soursop juice to decrease of LDL’s cholesterol levels in dyslipidemia white rat serum.This experiment used pre-test and post-test with control group design. Soursop juice doses used was at 0,9; 1,8 and 2,7 g / 200 g BW of white rat. The data were processed using descriptive and statistics by the Kolmogorov Smirnov Test, One Way ANOVA, Post Hoct Test and linear regression in SPSS 16.0 for Windows.The results of this experiment was the percentage decrease of LDL cholesterol levels in the white rat serum of various doses soursop juice, which is 0,9 g / 200 g BW of 46,19%, a dose of 1,8 g / 200 g BW of 52,30 % and a dose of 2,7 g / 200 g BW of 61,62%.There was the effect of various doses of soursop juice to decrease LDL’s cholesterol levels in dyslipidemia white rat serum. The greater the dose of soursop juice given, the greater the reduction in LDL’s cholesterol levels in the serum of dyslipidemia white rat.Consumption of excessive fat in the food that we consume daily can increase cholesterol, LDL cholesterol and triglycerides in the blood that is called dyslipidemia. Diet, exercise and drugs are the effort can be done to lower the lipid levels. The herbs have been widely used as an alternative to lower cholesterol levels because the active substances contained antihiperlipid properties. Soursop has many benefits in curing some diseases. Soursop contains an active ingredient that can lower LDL’s cholesterol levels, including vitamin C, fiber and niacin. Therefore, the researchers wanted to do experiment using soursop as an alternative for lowering LDL’s cholesterol levels in dyslipidemia white rat serum.The purpose of this experiment was to determine the effect of various doses of soursop juice to decrease of LDL’s cholesterol levels in dyslipidemia white rat serum.This experiment used pre-test and post-test with control group design. Soursop juice doses used was at 0,9; 1,8 and 2,7 g / 200 g BW of white rat. The data were processed using descriptive and statistics by the Kolmogorov Smirnov Test, One Way ANOVA, Post Hoct Test and linear regression in SPSS 16.0 for Windows.The results of this experiment was the percentage decrease of LDL cholesterol levels in the white rat serum of various doses soursop juice, which is 0,9 g / 200 g BW of 46,19%, a dose of 1,8 g / 200 g BW of 52,30 % and a dose of 2,7 g / 200 g BW of 61,62%.There was the effect of various doses of soursop juice to decrease LDL’s cholesterol levels in dyslipidemia white rat serum. The greater the dose of soursop juice given, the greater the reduction in LDL’s cholesterol levels in the serum of dyslipidemia white rat
Efektifitas Pemberian Perasan Bawang Putih (Allium Sativum Linn) Dosis Tunggal Terhadap Jumlah Telur Cacing Gelang (Toxocara Canis) Secara In Vivo
No full textBackground this research is worm disease problematic in the field of health. Treatment for the disease worms that have been used are synthetic drugs that have side effects are not good for health and can lead to dependence. One of the medicinal plants used for deworming is garlic. Chemical constituents in garlic is allicin that is anthelmintics.To prove that it is carried out in vivo study using dogs infected with Toxocara canis worms as test animals.Objective this research to knowing of the effectiveness of various doses of garlic juice with a gift on the first day against Toxocara canis worm egg counts in vivo and the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration of juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation.Methods this research is an experimental study using dogs infected with Toxocara canis nearly the same number of eggs as well as the research design pretest-posttest with control group.Results the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation known to occur on day 3 with a percentage of effectiveness are 48,49%; 67,18%; dan 82,85%. The higher doses of garlic juice, the more effective to decline in the amount of worm eggs Toxocara canis in vivo.Conclusion this research is garlic juice with various doses are not as effective as once treatment in reducing the the amount of eggs bracelet worm (Toxocara canis) in vivo