Jurnal Teknologi Laboratorium
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Pemanfaatan Ekstrak Daun Jambu Biji (Psidium guajava Linn) Sebagai Antibakteri dan Antifungi
No full textGuava (Psidium guajava Linn) is found throughout Indonesia. The leaves contain tannins that can be used as antibacterial and antifungal. The aims of this research are to determine the antiseptic power of guava leaf as antifungal and antibacterial.This research is pre experiments research with laboratory test to determine the inhibitory power of guava ethanol extract as anti-bacterial and anti-fungal. Guava leaves are old made 70% ethanol extract in LPPT UGM using maceration method. The extract made 3 concentrations ie 25%, 50% and 75%. Each concentration was tested for inhibitory by knowing the diameter of growth barrier to Candida albicans and Staphylococcus aureus. Each concentration is repeated 5 times, resulting in 15 data. The data were analyzed descriptively to illustrate their potential comparisons with chlorhexidine as standard materials. The extract of guava leaf using ethanol 70% in laboratory test resulted in average inhibitory zone diameter as follows: for mushroom C.albicans with extract 25%, 50%, and 75% were 13.4mm, 17.6mm, and 19.4mm. While for S. aureus is 2.2mm, 25.6mm, and 27.2mm. The effect of antifungal power of guava leaf extract (Psidium guajava Linn.) on the growth of Candida albicans fungus is smaller compared to Staphylococcus aureus bacteriaGuava (Psidium guajava Linn) is found throughout Indonesia. The leaves contain tannins that can be used as antibacterial and antifungal. To determine the antiseptic power of guava leaf as antifungal and antibacterial. Pre experiments with laboratory test to determine the inhibitory power of guava ethanol extract as anti-bacterial and anti-fungal. Guava leaves are old made 70% ethanol extract in LPPT UGM using maseration method. The extract made 3 concentrations ie 25%, 50% and 75%. Each concentration was tested for inhibitory by knowing the diameter of growth barrier to Candida albicans and Staphylococcus aureus. Each concentration is repeated 5 times, resulting in 15 data. The data were analyzed descriptively to illustrate their potential comparisons with chlorhexidine as standard materials. The extract of guava leaf using ethanol 70% in laboratory test resulted in average inhibitory zone diameter as follows: for mushroom C.albicans with extract 25%, 50% and 75% were 13.4mm, 17.6mm and 19.4mm. While for S. aureus is 2.2mm, 25.6mm, and 27.2mm. The effect of antifungal power of guava leaf extract (Psidium guajava Linn.) on the growth of Candida albicans fungus is smaller compared to Staphylococcus aureus bacteri
Identifikasi Gen Ctx-M Pada Esherichia Coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) Di RSUD Dr. Soetomo surabaya
No full textEscherichia coli producing Extended-Spectrum Beta-Lactamases (ESBLs) responsible for the high number of disease and death from nosocomial infections because of an enzyme encoded by genes CTX-M. This aim of the research to identify isolates E.coli of urine patients, which is a collection of Clinical Microbiology RSUD Dr. Soetomo Surabaya on the January-February 2014. The kind of the research use is observational descriptive with molecule approachment. The sample used in clinical isolate of E.coli producing ESBLs accumulated during January-February 2014 collection Clinical Laboratory RSUD Dr. Soetomo Surabaya that is thirty isolate. The methods used for the detection of genotypic by PCR thus electrophoresis and visualized on agarose gel 1.5%. The results show that twenty-seven isolate (90%) positive containing a gen CTX-M with the highest number found on the Interna Departement. Detection Bacteria producing ESBLs in genotype important that therapy an antibiotic the patients are given more effective and efficient.Esherichia coli producing Extended Spectrum Beta Lactamases (ESBLs) responsible for the high number of disease and death from nosocomial infections because of an enzyme encoded by genes CTX-M. This aim of the research to identity isolates E.coli of urine patients, which is a collection of Clinical Microbiology RSUD Dr. Soetomo Surabaya on the January-February 2014. The kind of the research use is observational descriptive with molecule approachment. The sample used is clinical isolate of E.coli producing ESBLs accumulated during January-February 2014 collection Clinical Laboratory RSUD Dr. Soetomo Surabaya that is thirty isolate. The methods used for the detection of genotypic by PCR thus electrophoresis and visualized on agarose gel 1.5%. The results show that twenty seven isolate (90%) positive containing a gen CTX-M with the highest number found on the Interna Departement. Detection Bacteria producing ESBLs in genotype important that therapy an antibiotic the patients given more effective and efficient
Pengembangan Mikroskop Dengan Mikrokontroler dan Cahaya Monokromatik Untuk Mendeteksi Parasit Malaria
No full textMalaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using polychromatic light source whether from halogen or natural light source. A microscopic technique has a lot of benefits but still have weaknesses, such as time-consuming and bias on the reading by microscopist, because of artifact in the image. Aims of this study were to designed malaria parasites detection tool that is robust, fast, convenient and clear by minimizing artifact on the slide. Design of this study was laboratory experimental which modified simple microscope into an automatic microscope with table movement and webcam recording using a microcontroller and monochromatic light source. The wavelength of the light sources was 402nm(blue), 532 nm (green) and 650 nm (red), the intensity of each source differed. The reading of the slide image was conducted by two certified microscopists, who read 60 images of a thick and thin slide with three different live stage of Plasmodium falciparum live, which wearing, trophozoite and schizont. This study showed that modification of microscope was succeeded with automatic movement and webcam recording, process time in one step movement and recording approximately 10 seconds or 17minutes for 100 fields of view as confirmation process. The monochromatic light source has proven to give a clear and contrast field of view when the intensities were higher than 40 mW and the certified microscopist able to identified Plasmodium falciparum parasites. Data analysis of microscopist reading used nonparametric statistic Friedman by SPSS showed that correlation between images using monochromatic and polychromatic lights have meaningless differences in a thick and thin slide. However, hemozoin as a marker of Plasmodium falciparum parasite was less detected by monochromatic light used in this study.Malaria masih menjadi masalah kesehatan yang utama diindonesia dan dunia, sampai saat ini teknik dalam mengidentifikasi parasit malaria masih menjadi tantangan peneliti untuk dikembangkan. Malaria pada manusia dapat dideteksi dengan menggunakan beberapa cara yaitu secara mikroskopis, serologi, PCR, dan teknik lainnya. Standar baku pemeriksaan malaria adalah mikroskopis dengan cahaya polikromatis yaitu lampu halogen atau Light Emitting Dioda (LED) yang mengidentifikasi morfologi dan jejak hemozoin pada tahapan lanjutan perkembangan parasite malaria. Teknik microscope sangat handal untuk dilakukan di daerah terpencil, namun kelemahan dari deteksi mikroskopis adalah kurang efisien waktu dan adanya artefak pada apusan darah dikarenakan objek dalam darah ataupun kotoran saat pembuatan apusan darah, hal ini sangat berpengaruh terhadap hasil analsis dari gambar. Penelitian ini bertujuan untuk merancang alat dengan memodifikasi mikroskop yang digabungkan dengan otomatisasi perekaman dan pergerakan meja preparat serta pencahayaan sinar laser untuk mengurangi waktu pembacaan dan mengurangi kesalahan baca akibat artefak pada slide. Penelitian dilakukan dalam dua tahap yaitu modifikasi mikroskop dengan pergerakan meja preparat menggunakan mikrokontroler dan perekaman webcam serta modifikasi pencahayaan menggunakan cahaya monokromatis dari cahaya Laser dengan panjang gelombang 402nm (violet), 532nm (hijau) dan 650nm (merah) dengan kekuatan intensitas yang berbeda. Hasil penelitian menunjukan bahwa modifikasi mikroskop dengan pergerakan meja preparat dan penggunaan cahaya Laser hijau dan laser biru dengan rentang intensitas diatas 40 mW dapat menghasilkan gambaran parasit yang baik dan dapat dibaca oleh analis mikroskopis. Hasil uji perbedaan dan uji korelasi secara statistik didapatkan bahwa gambaran morfologi parasit pada tiap-tiap hapusan darah yang dilihat menggunakan mikroskop dengan cahaya polikromatis menggunakan LED dan cahaya monokromatis didapatkan bahwa tidak cukup bermakna, baik pada hapusan darah tebal ataupun tipis
Pemeriksaan Angka Kuman Pada Daging Ayam Dengan Pemberian Parutan Rimpang Lengkuas Putih (Alpinia Galanga Linn Swartz)
No full textChicken meat is a good source of protein for daily consumption. It is very easy decayed biologically by enzymes or microbial spoilage. White galangal (Alpinia galanga Linn Swartz) is a kind of spice crop that can live in the highlands and lowlands. Generally, people utilize white galangal as a blend of seasoning. Galangal’s role as a food preservative is inseparable from its anti-microbial activity and secondary metabolite contents, i.e. essential oils. The anti-microbial is a biological or chemical compounds that could interfere the growth and activity of microbes, particularly microbes as a food spoilage. This research goal is to determine the number of bacteria in chicken meat with the provision granting the white grated galangal rhizome (Alpinia galanga Linn Swartz).This was a descriptive study with laboratory testing. We use pour plate method for the bacteria number determination. Independent variables is the indwelling time with grated white galangal for 1-5 hours and the dependent variable is the number of bacteria in chicken meat.The result showed that total number of bacteria after smeared with white grated galangal rhizome for 1 hour 463.500 CFU/gr, 2 hour 130.250 CFU/gr, 3 hour 58.250 CFU/gr, 4 hour 142.500 CFU/gr and 5 hour 302.500 CFU/gr. This study showed that grated white galangal has proven to reduce the number of bacteria in chicken meat.Chicken meat is a good source of protein for daily consumption. It is very easy decayed biologically by enzymes or microbial spoilage. White galangal (Alpinia galanga Linn Swartz) is a kind of spice crop that can live in the highlands and lowlands. Generally, people utilize white galangal as a blend of seasoning. Galangal’s role as a food preservative is inseparable from its anti-microbial activity and secondary metabolite contents, i.e. essential oils. The anti-microbial is a biological or chemical compounds that could interfere the growth and activity of microbes, particularly microbes as a food spoilage. This research goal is to determine the number of bacteria in chicken meat with the provision granting the white grated galangal rhizome (Alpinia galanga Linn Swartz).This was a descriptive study with laboratory testing. We use pour plate method for the bacteria number determination. Independent variables is the indwelling time with grated white galangal for 1-5 hours and the dependent variable is the number of bacteria in chicken meat.The result showed that total number of bacteria after smeared with white grated galangal rhizome for 1 hour 463.500 CFU/gr, 2 hour 130.250 CFU/gr, 3 hour 58.250 CFU/gr, 4 hour 142.500 CFU/gr and 5 hour 302.500 CFU/gr. This study showed that grated white galangal has proven to reduce the number of bacteria in chicken meat
Identifikasi Candida Albican Pada Saliva Wanita Penderita Diabetes Melitus
No full textoai:ojs2.www.teknolabjournal.com:article/44Diabetes mellitus is a predisposing factor for the onset of oral candidiasis. Women with diabetes have extra sugar in the secretion of saliva. Sugar contained in saliva are stacked on the mucosa so provide food for mold growth so that the diabetic patient saliva will be found Candida albicans The aim of this study was to identify Candida albicans in saliva of women with diabetes mellitus in patient wards of Medicine Hospital Dr M.Yunus Bengkulu 2017.This type of research is conducted by using descriptive survey design. Sampling technique using Purposive Sampling, the number of samples identified as many as 27, conducted by macroscopic and microscopic examination. Data in this research using descriptive analysis.Results of univariate analysis obtained, all respondents the majority of respondents 52% positive saliva fungus Candida albicans and almost half of respondents 48% negative saliva fungus Candida albicans. The prevalence values indicate that the majority of the total population of women with DM saliva samples contained fungus the Candida albicans. Whole saliva respondents (100%) in women with diabetes is growing colonies on Media SDA (Saboraud Dextrose Agar) fungus Candida albican
CEMARAN Staphylococcus aureus DAN Pseudomonas aerogenosa PADA STETESKOP DIRUMAH SAKIT
No full textMedical devices that are susceptible to bacteria contaminated one of them is a stethoscope. Nosocomial infections that are problematic in the hospital include Staphylococcus aureus and Pseudomonas aerogenosa. These bacteria can cause mild skin infections to serious life-threatening infections. The purpose of this research is to know the existence of bacteria contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop at Banjarbaru hospital. This research uses descriptive survey method, which is a research method to describe objectively based on facts found. The samples were stethoscope in child care room, internal medicine room, delivery room, post partum room, perinatology room, and post-surgical treatment room at Banjarbaru hospital for 30 stethoscopes.The result of the research was found contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop. The site home condition of the research data was 66.7% cleaned daily, the storage method was placed on the table 70% and the duration of using the set home more than 1 year as much as 70%. The conclusion of stethoscope at Banjarbaru Hospital was contaminated with Staphylococcus aureus by 70% and Pseudomonas aerogenosa by 17%. The suggestion of research can be continued by knowing the existence of Staphylococcus aureus resistant antibiotic and Pseudomonas aerogenous antibiotic resistant at steteskop at Hospital.Medical devices that are susceptible to bacteria contaminated one of them is a stethoscope. Nosocomial infections that are problematic in the hospital include Staphylococcus aureus and Pseudomonas aerogenosa. These bacteria can cause mild skin infections to serious life-threatening infections. The purpose of this research is to know the existence of bacteria contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop at Banjarbaru hospital. This research uses descriptive survey method, which is a research method to describe objectively based on facts found. The samples were stethoscope in child care room, internal medicine room, delivery room, post partum room, perinatalogi room, and post-surgical treatment room at Banjarbaru hospital for 30 stethoscopes.
The result of the research was found contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop. The stethome condition of the research data was 66.7% cleaned daily, the storage method was placed on the table 70% and the duration of using the stethome more than 1 year as much as 70%. The conclusion of stethoscope at Banjarbaru Hospital was contaminated with Staphylococcus aureus by 70% and Pseudomonas aerogenosa by 17%. Suggestion of research can be continued by knowing the existence of Staphylococcus aureus resistant antibiotic and Pseudomonas aerogenous antibiotic resistant at steteskop at Hospita
Gambaran Kadar Trigliserida (Metode Gpo-Pap) Pada Sampel Serum dan Plasma EDTA
No full textHasil Laboratorium yang bermutu harus memiliki ketepatan dan ketelitian tinggi. Pemeriksaan laboratorium sangat diperlukan untuk membantu dalam menegakkan diagnosa suatu penyakit secara akurat menurut ukuran medis. Pemeriksaan kadar trigliserid dapat membantu perubahan pola dan gaya hidup sehat, dapat dihindari mengonsumsi makanan yang mengandung karbohidrat atau kadar gula yang tinggi berisiko terkena penyakit jantung dan stroke akan meningkat seiring dengan tingginya kadar trigliserida seseorang. Pemeriksaan untuk menentukan kadar trigliserid biasanya menggunakan sampel serum dan plasma. Mengetahui hasil pemeriksaan kadar trigliserid dan gambaran perbedaan hasil kadar trigliserid metode GPO-PAP dari sampel serum dan plasma EDTA. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian deskriptif disajikan dalam bentuk tabel untuk mengetahui selisih rata-rata kadar trigliserid sampel serum dan plasma EDTA dan grafik untuk mengetahui yang lebih tinggi. Hasil pemeriksaan kadar trigliserid dengan sampel serum rata – rata 143,16 mg/dl sedangkan pemeriksaan kadar trigliserid dengan sampel plasma rata – rata 142,16 mg/dl. Kesimpulan adalah ada perbedaan hasil pemeriksaan kadar trigliserid metode GPO-PAP antara sampel serum dan plasma EDTA adalah 24%.Hasil Laboratorium yang bermutu harus memiliki ketepatan dan ketelitian tinggi. Pemeriksaan laboratorium sangat diperlukan untuk membantu dalam menegakkan diagnosa suatu penyakit secara akurat menurut ukuran medis. Pemeriksaan kadar trigliserid dapat membantu perubahan pola dan gaya hidup sehat, dapat dihindari mengonsumsi makanan yang mengandung karbohidrat atau kadar gula yang tinggi berisiko terkena penyakit jantung dan stroke akan meningkat seiring dengan tingginya kadar trigliserida seseorang. Pemeriksaan untuk menentukan kadar trigliserid biasanya menggunakan sampel serum dan plasma. Mengetahui hasil pemeriksaan kadar trigliserid dan gambaran perbedaan hasil kadar trigliserid metode GPO-PAP dari sampel serum dan plasma EDTA. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian deskriptif disajikan dalam bentuk tabel untuk mengetahui selisih rata-rata kadar trigliserid sampel serum dan plasma EDTA dan grafik untuk mengetahui yang lebih tinggi. Hasil pemeriksaan kadar trigliserid dengan sampel serum rata – rata 143,16 mg/dl sedangkan pemeriksaan kadar trigliserid dengan sampel plasma rata – rata 142,16 mg/dl. Kesimpulan adalah ada perbedaan hasil pemeriksaan kadar trigliserid metode GPO-PAP antara sampel serum dan plasma EDTA adalah 24%
Gambaran Perbedaan Kadar Kolesterol Total Metode CHOD-PAP (Cholesterol Oxidase – Peroxsidase Aminoantypirin) Sampel Serum dan Sampel Plasma EDTA
No full textPemeriksaan laboratorium sangat diperlukan untuk membantu menegakkan diagnosa suatu penyakit dan memperoleh hasil pemeriksaan yang akurat. Pemeriksaan kadar kolesterol dapat membantu perubahan pola dan gaya hidup sehat, untuk menghindari makanan yang mengandung kolesterol tinggi berisiko meningkatkan kadar kolesterol darah. Pemeriksaan kadar kolesterol biasanya menggunakan sampel serum dan sampel plasma. Penelitian ini bertujuan untuk mengetahui hasil pemeriksaan kadar kolesterol dan gambaran perbedaan hasil kadar kolesterol total metode CHOD-PAP dari sampel serum dan plasma EDTA. Jenis penelitian yang dilakukan adalah uji deskriptif disajikan dalam bentuk tabel untuk mengetahui selisih rata-rata kadar kolesterol total sampel serum dan plasma EDTA dan grafik untuk mengetahui yang lebih tinggi. Hasil uji deskriptif didapatkan kadar kolesterol total dengan rata – rata sampel serum 157,76 mg/dl, sedangkan pemeriksaan kadar kolesterol dengan rata – rata sampel plasma EDTA 153,71 mg/dl. Berdasarkan data penelitian analisa uji deskriptif dinyatakan perbedaan hasil pemeriksaan kadar kolesterol total antara sampel serum dan plasma EDTA yaitu 9,7 %.Pemeriksaan laboratorium sangat diperlukan untuk membantu menegakkan diagnosa suatu penyakit dan memperoleh hasil pemeriksaan yang akurat. Pemeriksaan kadar kolesterol dapat membantu perubahan pola dan gaya hidup sehat, untuk menghindari makanan yang mengandung kolesterol tinggi berisiko meningkatkan kadar kolesterol darah. Pemeriksaan kadar kolesterol biasanya menggunakan sampel serum dan sampel plasma. Penelitian ini bertujuan untuk mengetahui hasil pemeriksaan kadar kolesterol dan gambaran perbedaan hasil kadar kolesterol total metode CHOD-PAP dari sampel serum dan plasma EDTA. Jenis penelitian yang dilakukan adalah uji deskriptif disajikan dalam bentuk tabel untuk mengetahui selisih rata-rata kadar kolesterol total sampel serum dan plasma EDTA dan grafik untuk mengetahui yang lebih tinggi. Hasil uji deskriptif didapatkan kadar kolesterol total dengan rata – rata sampel serum 157,76 mg/dl, sedangkan pemeriksaan kadar kolesterol dengan rata – rata sampel plasma EDTA 153,71 mg/dl. Berdasarkan data penelitian analisa uji deskriptif dinyatakan perbedaan hasil pemeriksaan kadar kolesterol total antara sampel serum dan plasma EDTA yaitu 9,7 %
Gambaran Kadar Kolesterol-LDL (Low Density Lipoprotein) Sebelum dan 48 Jam Sesudah Melakukan Satu Kali Terapi Bekam Basah Pada Penderita Hipertensi Dengan Pola lima titik
No full textHypertension, or more commonly known as high blood pressure is a condition in which a person got an increasing blood pressure upper normal, resulting in increasing morbidity and mortality. The long hypertension is one risk factor for cardiovascular disease, which is one cause of atherosclerosis. Atherosclerosis is a very progressive diseases that causes hardening of the arteries due to the blockage by oxidized cholesterol. Atherosclerosis begins with the build up of LDL-cholesterol. There are two handling of LDL-cholesterol; pharmacological and non-pharmacological. Nonpharmacologic is done with wet cupping therapy. The aim of this study is to determine the average LDL-cholesterol levels before and after the wet cupping therapy with five-point pattern. This research is descriptive research, then presented in the form of tables to showing the results of the study. This study was done Talunombo, Sidomulya, Pengasih, Kulon Progo. This research object is venous blood samples taken from hypertensive patients as research subjects. Descriptive test results obtained an average LDL-cholesterol levels before the wet cupping therapy is 114,182 mg/dl and after wet cupping is 115,618 mg/dl. The conclusion of this study is the average LDL-cholesterol levels prior to the wet cupping therapy with a five-point pattern is 114,182 mg/dl and after wet cupping with five-point pattern is 115,618 mg/dl.Hypertension, or more commonly known as high blood pressure is a condition in which a person got an increasing blood pressure upper normal, resulting in increasing morbidity and mortality. The long hypertension is one risk factor for cardiovascular disease, which is one cause of atherosclerosis. Atherosclerosis is a very progressive diseases that causes hardening of the arteries due to the blockage by oxidized cholesterol. Atherosclerosis begins with the build up of LDL-cholesterol. There are two handling of LDL-cholesterol; pharmacological and non-pharmacological. Nonpharmacologic is done with wet cupping therapy. The aim of this study is to determine the average LDL-cholesterol levels before and after the wet cupping therapy with five-point pattern. This research is descriptive research, then presented in the form of tables to showing the results of the study. This study was done Talunombo, Sidomulya, Pengasih, Kulon Progo. This research object is venous blood samples taken from hypertensive patients as research subjects. Descriptive test results obtained an average LDL-cholesterol levels before the wet cupping therapy is 114,182 mg/dl and after wet cupping is 115,618 mg/dl. The conclusion of this study is the average LDL-cholesterol levels prior to the wet cupping therapy with a five-point pattern is 114,182 mg/dl and after wet cupping with five-point pattern is 115,618 mg/dl
Gambaran Kadar Kolesterol Total Pada Penderita Hipertensi Sebeum Dan Sesudah Terapi Bekam Basah
No full textHipertensi merupakan faktor resiko utama dari perkembangan penyakit jantung dan stroke. Penyakit hipertensi berkembang secara perlahan, tetapi secara potensial sangat berbahaya. Hipertensi biasanya dikuti dengan penyakit lain yang menyertainya seperti hiperlipidemia Salah satu profil lipid yang dapat diukur adanya hiperlipidemia adalah kadar kolesterol total. Upaya pengendalian hipertensi yang disebabkan kadar kolesterol total yang tinggi yaitu dengan cara pengobatan baik sercara farmakologi maupun non-farmakologi. Pengobatan non-farmakologi salah satunya adalah terapi bekam basah.Penelitian ini bertujuan untuk mengetahui selisih rata-rata kadar kolesterol total pada penderita hipertensi sebelum, sesudah diberikan terapi bekam basah pertama dan kedua dengan pola tiga titik. Jenis penelitian yang digunakan adalah pre-experimental dengan desain penelitian pre and post test without control yaitu kadar kolesterol total sebelum dilakukan terapi bekam basah adalah pre test, sedangkan kadar kolesterol total setelah dilakukan terapi bekam basah adalah post test. Hasil didapatkan rata – rata kadar kolesterol total sebelum dilakukan terapi bekam basah adalah 194,89 mg/dl, setelah dilakukan terapi bekam basah pertama sebesar 200,89 mg/dl dan setelah dilakukan terapi bekam basah kedua menjadi 200, 33 mg/dl. Kesimpulann dari penelitian ini adalah selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah pertama adalah 6,00 mg/dl atau mengalami kenaikan sebesar 3,08% sedangkan selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah kedua adalah 5,44 mg/dl atau mengalami kenaikan sebesar 2,79%.Hipertensi merupakan faktor resiko utama dari perkembangan penyakit jantung dan stroke. Penyakit hipertensi berkembang secara perlahan, tetapi secara potensial sangat berbahaya. Hipertensi biasanya dikuti dengan penyakit lain yang menyertainya seperti hiperlipidemia Salah satu profil lipid yang dapat diukur adanya hiperlipidemia adalah kadar kolesterol total. Upaya pengendalian hipertensi yang disebabkan kadar kolesterol total yang tinggi yaitu dengan cara pengobatan baik sercara farmakologi maupun non-farmakologi. Pengobatan non-farmakologi salah satunya adalah terapi bekam basah.Penelitian ini bertujuan untuk mengetahui selisih rata-rata kadar kolesterol total pada penderita hipertensi sebelum, sesudah diberikan terapi bekam basah pertama dan kedua dengan pola tiga titik. Jenis penelitian yang digunakan adalah pre-experimental dengan desain penelitian pre and post test without control yaitu kadar kolesterol total sebelum dilakukan terapi bekam basah adalah pre test, sedangkan kadar kolesterol total setelah dilakukan terapi bekam basah adalah post test. Hasil didapatkan rata – rata kadar kolesterol total sebelum dilakukan terapi bekam basah adalah 194,89 mg/dl, setelah dilakukan terapi bekam basah pertama sebesar 200,89 mg/dl dan setelah dilakukan terapi bekam basah kedua menjadi 200, 33 mg/dl. Kesimpulann dari penelitian ini adalah selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah pertama adalah 6,00 mg/dl atau mengalami kenaikan sebesar 3,08% sedangkan selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah kedua adalah 5,44 mg/dl atau mengalami kenaikan sebesar 2,79%