JURNAL PESISIR DAN LAUT TROPIS
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BAKTERI RESISTEN ARSEN (As) PADA ALGA Padina australis DARI PERAIRAN KIMA BAJO
Get PDFPadina australis is a species of marine algae from the Phaeophyta division (brown algae) which is generally distributed in marine waters. This algae is able to live in waters with high levels of heavy metals such as in the Kima Bajo waters. This study aims to isolate arsenic (As)-resistant bacteria in P. australis, observe cell shape and bacterial characteristics, and determine bacterial resistance to antibiotics. The method used is conventional bacterial isolation which has been tested on As2O3 compounds. The results obtained were that P. australis contained arsenic resistant bacteria at concentrations of 250 ppm, 500 ppm, and 1000 ppm. The bacteria obtained were Gram positive, in the form of bacilli. These arsenic-resistant bacteria are also resistant to antibiotics such as amoxicillin, cefixime, and doxycycline.Keywords: Padina australis, Arsen (As), Kima Baj
KEANEKARAGAMAN DAN KEPADATAN ECHINOIDEA DAN ASTEROIDEA DI RATAAN TERUMBU KARANG PERAIRAN TONGKAINA DAN BAHOWO KOTA MANADO (
Get PDFThe waters of Tongkaina, which are part of northern Manado's waters, are known to have mangrove, seagrass, and seaweed ecosystems. Therefore efforts are needed to manage the population and diversity of echinoderms in these waters. Echinoderms' ecology has an essential role in maintaining the marine ecosystem's balance, which plays a role in recycling nutrients. This study aimed to determine diversity, species density, species relative density, species frequency, species relative frequency, dominance, and the index of importance of Echinoidea and Asteroidea's species in the waters of the Tongkaina Village and their density. The method used in this research is the quadratic line transect method. First of all, the survey was conducted to the sampling site's location in the coral reef flats of Tongkaina Village's waters, including Tongkaina beach and Bahowo beach, before operating the sampling. Then the observation stations were determined, namely Tongkaina waters (near Batu Meja) and Bahowo waters. The quadratic line transect method is used in this research. Species of class Echinoidea and Asteroidea in the quadrat were recorded among the total of individuals, and the species is documented. This study's results indicate that six species were found, including the survey, and five species were found based on the line transect method with 182 individuals. Including Asteroidea 85 Individuals and Echinoidea 99 Individuals. Value of Diversity at station 1 has a value of H '= 1.170 with a dominance of C = 0.364, while at Station 2 has a value of H' = 1.232 and dominance value of C = 0.316. the highest density in Station 1 is Diadema savigny 1.967 ind/m2 relative 50.86%, and Linckia laevigata with the highest frequency Fi = 0.533. The highest important value index is Diadema savigny, with a value of 81.09%, while at station 2, the Protoreaster nodosus has the highest value of important value index with a value of 87.51%. These species also have the highest density of 0.933 ind/m2 and a relative number of 41.79%.Keywords: Tongkaina, Echinoderm, Line Transect Quadrat Method, Asteroidea, Echinoidea, Coral Reef Fla
KERAGAMAN KELINCI LAUT (NUDIBRANCHIA) DI PERAIRAN LAUT SULAWESI UTARA
Get PDFThis study aims to 1) identify the species of Nudibranchia in North Sulawesi marine waters, especially in Malalayang waters, Teep waters, Sangihe waters, Bunaken waters, Gangga waters and Lembeh straits, 2) find out species of Nudibranchia that is found in all sites, and 3) find out index community structure of Nudibranchia in all sites.  The result show that there were 11 species of Nudibranchia in Malalayang waters, 27 species in Lembeh Strait, 11 species in Teep waters, 57 species in Bunaken waters, 91 species in Bangka waters, and 20 species in Sangihe waters. Up to now, there were 139 species of Nudibranchia in North Sulawesi marine waters, where Species of Phyllidiella pustulosa, Phyllidia ocellata, Phyllidia varicose, Chromodoris annae, and Glossodoris cincta were found in all study locations. The species of Doriprismatica atromarginata was only found in the Malalayang waters. A high Index Diversity was found in Bunaken and Bangka waters, and a middle Index Diversity was found in the waters of Lembeh Strait, Teep, and Sangihe. A stable community structure was found for all locations, while a low domination was categorized to all locations. Keywords: Nudibranchia, species, diversity, evenness, domination, and community structure
Pemberdayaan Kelompok Nelayan Desa Arakan Kabupaten Minahasa Selatan Dalam Diversifikasi Produk Olahan Ikan
Get PDFThe purpose of this program is to empower the Women Fishermen group in Arakan Village, South Minahasa Regency, North Sulawesi Province in processing / diversifying fish products into shredded. This diversification of shredded fish is expected to increase production, management to be skilled and economically independent which in turn can increase people's income. The specific target of this activity is to produce shredded fish products that are superior in terms of taste, sanitation and hygiene, durability, and create jobs / businesses and strong management. The implementation methods applied to this program are 1) the production of shredded fish; 2) Approach methods to overcome partner problems, namely counseling, training, mentoring and evaluation; 3) Work procedures to support aspects of the problem, respectively, namely survey, counseling, training, evaluation, output, reporting.The result of this activity is an increase in the skills and knowledge or empowerment of the Women Fishermen group in Arakan Village, South Minahasa Regency, North Sulawesi Province in processing/diversifying fishery products, namely fish floss which is delicious and favored by panelists, packaged practically. This activity is expected; women in coastal areas can be empowered through economic activities based on fishery business so that they become a new locomotive in bringing the economy of fishing communities to be better. Keywords: empowerment, diversifying processed, fish products, Arakan villag
SIPUT PROSOBRANCHIA (GASTROPODA, MOLUSKA) PADA RATAAN TERUMBU KARANG DI TELUK MANADO
Get PDFMolluscs are marine organisms that do not have a backbone, these organisms are associated with coral reefs. This research was conducted with the aim of describing the density and diversity of Prosobranchia snails’ species on coral reef flats. By applying the belt transect method, the results show that the density of the four Mokupa stations has a relatively high density between Tongkeina, Malalayang and Bulo. While for species diversity, Mokupa and Bulo had the highest value than Tongkeina and Malalayang. According to the results of the belt transect method, is the concluded that stations close to people’s homes have the lowest density and species diversity values, this factor is influenced by anthropogenic activities or human activities. Therefore, it is recommended that there be monitoring of snails on coral reefs, especially reef flattening in order to provide information about Prosobranchia snails.Keywords: Prosobranchia, Density, diversity, belt transects, Reef Flat
ANALISIS STRUKTUR KOMUNITAS MANGROVE DI KAWASAN SEKITAR PT. CONCH KABUPATEN BOLAANG MONGONDOW
Get PDFThis research was conducted for four months of April-July 2020. The purpose of this study was to determine species density, species relative density, species frequency, species relative frequency, species cover, species relative cover, index of the importance of mangrove species in the area around PT. Conch Lolak District, Bolaang Mongondow Regency, North Sulawesi Province. This research was conducted for four months of April-July 2020. The method used was a quadratic plot of 6 stations. Data collection at each station was made with a plot size of 10 × 10 m2 to measure the diameter of a tree at breast height (DBH), which has a minimum trunk circumference of 16 cm. The results showed that there were five types of mangroves, namely R. apiculata, R. mucronata, B. gymnorhiza, S. alba, and X. granatum. The highest relative density of species at station 2, namely R. mucronate 62.96%, while the lowest value at station 3, namely R. mucronata 8.77%, the highest relative frequency value at station 4, namely R. mucronata 33.33%, whereas the lowest value was at station 5, namely B. gymnorhiza 25.00%, the highest relative closure value was at station 5, namely R. mucronata 30.91%, while the lowest was at station 2, namely R. apiculata 12.88%, and the important value index the highest was at station 3, namely R. apiculata 138.09% and the lowest value was at station 5, namely X. granatum 35.05%.Kata Kunci: Mangrove, Structure in areas around PT. Conch. Conch, Rhizopora Abstrak Penelitian ini dilakukan selama 4 bulan April-Juli 2020. Tujuan penelitian ini adalah untuk mengetahui kerapatan jenis, kerapatan relatif jenis, frekuensi jenis, frekuensi relatif jenis, penutupan jenis, penutupan relatif jenis, indeks nilai penting jenis mangrove yang ada di kawasan sekitar PT. Conch Kecamatan Lolak, Kabupaten Bolaang Mongondow, Provinsi Sulawawesi Utara. Penelitian ini dilakukan selama 4 bulan April-Juli 2020. Metode yang dipakai adalah plot kuadrat sebanyak 6 stasiun. Pengambilan data pada setiap stasiun dibuat plot dengan ukuran 10 × 10 m2 untuk mengukur diameter pohon setinggi dada (DBH) yang memiliki lingkar batang minimal 16 cm. Hasil penelitian menunjukkan bahwa ada 5 jenis mangrove yaitu R. apiculata, R. mucronata, B. gymnorhiza, S. alba dan X. granatum. Kerapatan relatif jenis tertinggi pada stasiun 2, yaitu R. mucronata 62,96%, sedangkan nilai terendah pada stasiun 3, yaitu R. mucronata 8,77%, nilai Frekuensi relatif jenis tertingi pada stasiun 4, yaitu R. mucronata 33.33%, sedangkan nilai terendah pada stasiun 5, yaitu B. gymnorhiza 25.00%, nilai Penutupan relatif jenis tertinggi pada stasiun 5, yaitu R. mucronata 30,91%, sedangkan terendah pada stasiun 2, yaitu R. apiculata 12,88%, dan Indeks nilai penting tertinggi pada stasiun 3, yaitu R. apiculata 138,09% dan nilai terendah pada stasiun 5, yaitu X. granatum 35,05%.Kata Kunci: Mangrove, kawasan sekitar PT. Conch, Rhizopora Â
AKTIVITAS SENYAWA ANTIBAKTERI DAN ANTI-UV DARI Phyllidia varicosa (Cuvier, 1804) DAN BAKTERI SIMBIONNYA (NUDIBRANCHIA GASTROPODA) DARI PERAIRAN TANJUNG MANDOLANG, MINAHASA
Get PDFPhyllidia varicosa is an organism belongs to the order Nudibranchia, which is commonly known as a sea rabbit. This organism is able to synthesize secondary metabolites from food. The purpose of this study was to obtain P. varicosa extract and symbiotic bacterial extract from P. varicosa, then determine the antibacterial activity of P. varicosa extract and the symbionic bacteria extract against Escherichia coli DSM498, Bacillus megaterium DSM32T and anti-UV activity. P. varicose symbiotic bacterial were isolated and extracted. The results obtained 5 bacterial isolates. The results of antibacterial assay of isolates PhVa 1.1, PhVa 1.3, PhVa 2.1, PhVa 2.3 and PhVa 2.4 shown that these isolates have an antibacterial activity against E. coli DSM498 and B. megaterium DSM32T. Anti-UV assay results shown an absorption at UV-A with the highest value of 1.991 at λ 340 nm. Keywords: Nudibranchia, Phyllidia varicosa, Antibacterial, Anti-UV, Escherichia coli, Bacillus megaterium Abstrak Phyllidia varicosa merupakan organisme yang termasuk dalam ordo Nudibranchia, yang umumnya dikenal sebagai kelinci laut. Organisme ini mampu mensintesis metabolit sekunder dari bahan makanannya. Tujuan dari penelitian ini yaitu untuk mendapatkan ekstrak P. varicosa dan ekstrak bakteri simbion dari P. varicosa, kemudian mengamati aktivitas antibakteri dari ekstrak P. varicosa dan ekstrak bakteri simbionnya terhadap bakteri Escherichia coli DSM498 dan Bacillus megaterium DSM32T serta menguji aktivitas anti-UV. Bakteri yang bersimbion dengan P. varicosa diisolasi dan diekstraksi, lalu diuji bioaktivitas antibakteri dan diuji anti-UV terhadap ekstrak P. varicosa dan ekstrak bakteri simbionnya. Hasil akhir dari penelitian ini yaitu didapatkan 5 isolat bakteri. Hasil uji aktivitas antibakteri yaitu isolat PhVa 1.1, PhVa 1.3, PhVa 2.1, PhVa 2.3 dan PhVa 2.4 memiliki aktivitas antibakteri terhadap bakteri E. coli DSM498 dan B. megaterium DSM32T. Hasil uji anti-UV menunjukkan serapan pada UV-A dengan nilai tertinggi 1,991 pada λ 340 nm. Kata kunci: Nudibranchia, Phyllidia varicosa, Antibakteri, Anti-UV, Escherichia coli, Bacillus megateriu
BAKTERI PADA KARANG SCLERACTINIA DI KAWASAN PERAIRAN BUNAKEN, MOROTAI DAN RAJA AMPAT
Get PDFResearch on hard coral (Scleractinian coral) contaminated with bacteria is still not much done, especially in Indonesian waters. This study took samples of coral mucus in 2010 at 3 (three) different locations, namely Bunaken (May); Morotai (September) and Raja Ampat (November), which focused on the analysis of Research on hard coral (Scleractinian coral) contaminated with bacteria is still not much done, especially in Indonesian waters. This study took samples of coral mucus in 2010 at 3 (three) different locations, namely Bunaken (May); Morotai (September) and Raja Ampat (November), which focused on the analysis of gram-positive and gram-negative bacteria. The method used for field sampling is time swim, which is by diving at a depth of 5-10 meters for ± 30 minutes and randomly taking samples of coral mucus using siring or by taking directly on corals (reef branching). Mucus samples were analyzed by bacterial isolation in the laboratory. The result shows that there were differences between gram-positive and gram-negative bacteria in the three research sites and that gram-positive bacteria were higher or dominant. Further research that can identify the bacteria species and explain its relationship to the ecosystem is highly recommended.Keywords: Bacteria, Scleractinian coral, gram-positive and -negative, Bunaken, Morotai, Raja Ampat                         AbstrakPenelitian tentang karang keras (Scleractinian coral) yang terkontaminasi bakteri masih belum banyak dilakukan, terutama di perairan Indonesia. Penelitian ini mengambil sampel mucus karang pada tahun 2010 di 3 (tiga) lokasi berbeda, yakni Bunaken (Mei); Morotai (September) dan Raja Ampat (November), yang difokuskan pada analisis bakteri gram postif dan gram negatif. Metode yang digunakan untuk pengambilan sampel di lapangan adalah time swim, yaitu dengan penyelaman pada kedalaman 5-10 meter selama ±30 menit dan mengambil sampel mucus karang secara acak menggunakan siring atau dengan mengambil langsung pada karang (fraksi cabang). Sampel mucus dianalisis dengan cara isolasi bakteri di laboratorium. Hasil analisis menunjukkan bahwa ada perbedaan antara bakteri gram positif dan gram negative di tiga lokasi survei dan bakteri gram positif lebih tinggi atau dominan. Penelitian lebih lanjut yang dapat menentukan jenis bakteri serta menjelaskan hubungannya dengan ekosistem sangat disarankan untuk dilakukan.Kata Kunci : Bakteri, Scleractinian coral, gram positif dan negatif, Bunaken, Morotai, Raja Ampa
AMPLIFIKASI GEN 16S-rRNA BAKTERI EPIFIT PADA ALGA MERAH Kappaphycus alvarezii
Get PDFBacteria are microscopic organism found living in marine algae. So far, species of bacteria in marine algae are not well known. In this study, epiphytic bacteria in algal species of Kappaphycus alvarezii (red algae) were isolated to amplify their 16S-rRNA gene. Sample K.alvarezii was collected from the island of Nain. The isolated epiphytic bacteria from the red algae K.alvarezii were grown in Nutrient Broth (NB) media. DNA extraction was carried out using InnuPREP DNA Mini Kit. 16SrRNA genes was performed using primer pair of 8F and 1492R. Two different character of epiphytic bacteria were successfully isolated from K. alvarezii.  16S-rRNA genes from the two isolates was successfully amplified, indicated by the presence of DNA band in each sample gel electrophoreses at around 1500 bp.Keywords : Amplification, 16S-rRNA Genes, Kappapchycus alvarezii, Bacterial, Epiphytic  Abstrak Bakteri adalah organisme mikroskopis yang ditemukan hidup di alga laut. Sejauh ini, spesies bakteri di alga laut belum dikenal. Dalam penelitian ini, bakteri epifit pada spesies alga Kappaphycus alvarezii (alga merah) diisolasi untuk memperkuat gen 16S-rRNA mereka. Sampel K.alvarezii dikumpulkan dari pulau Nain. Bakteri epifit terisolasi dari alga merah K.alvarezii ditanam dalam media Nutrient Broth (NB). Ekstraksi DNA dilakukan dengan menggunakan InnuPREP DNA Mini Kit. Gen 16S-rRNA dilakukan dengan menggunakan pasangan primer 8F dan 1492R. Dua karakter bakteri epifit yang berbeda berhasil diisolasi dari K. alvarezii. Gen 16S-rRNA dari dua isolat berhasil diamplifikasi, ditunjukkan oleh adanya pita DNA pada setiap sampel gel elektroforesis sekitar 1500 bp. Kata Kunci: Amplifikasi, gen 16S-rRNA, Kappapycus alvarezii, Bakteri, Epifi
KEKAYAAN DAN POTENSI SENYAWA BIOAKTIF MAKROALGA DI PESISIR ATEP OKI, KABUPATEN MINAHASA, SULAWESI UTARA
Get PDFThis study aims to analyze the richness and potential of macroalgae bioactive compounds on the Atep Oki Coast, District of Minahasa, North Sulawesi Province. This research was conducted in October 2019 until January 2020. Sampling used the roaming method in a predetermined location that is Atep Oki tidal/ intertidal coast. Sampling locations are spread over six coordinate points. Macroalgae found on the Atep Oki Coast assessed ten species belonging to three phyla, namely Chlorophyta (green algae), Ochrophyta (brown algae) and Rhodophyta (red algae). Chlorophyta members mean six species. Ochrophyta has members of three species and members of Rhodophyta only one species. The types of macroalgae found on the Atep Oki Coast provide bioactive compounds. Keywords : Macroalgae, Bioactive Compounds, Atep Oki Minahasa CoastAbstrak Penelitian ini bertujuan untuk menganalisis kekayaan jenis dan potensi senyawa bioaktif makroalga di Pesisir Atep Oki, Kabupaten Minahasa, Provinsi Sulawesi Utara. Penelitian ini telah dilaksanakan pada bulan Oktober 2019 sampai Januari 2020. Pengambilan sampel menggunakan metode jelajah di lokasi yang telah ditentukan yaitu pesisir pasang surut/intertidal Atep Oki. Lokasi pengambilan sampel tersebar di enam titik-titik koordinat. Makroalga yang ditemukan di Pesisir Atep Oki berjumlah sepuluh species yang tergolong ke dalam tiga filum yaitu Chlorophyta (alga hijau), Ochrophyta (alga cokelat) dan Rhodophyta (alga merah). Anggota Chlorophyta berjumlah enam species. Ochrophyta memiliki anggota tiga species dan anggota Rhodophyta hanya satu species. Jenis-jenis makroalga yang ditemukan di Pesisir Atep Oki berpotensi menghasilkan senyawa bioaktif. Kata kunci : Makroalga, Senyawa Bioaktif, Pesisir Atep Oki Minahas