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    Morphology of nematodes belonging to the genus Rictularia(Nematoda: Rictulariidae) collected from Apodemus agrarius in Shenyang, China

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    ArticleThe morphology of the rictulariid nematode species collected from Apodemus agrarius in Shenyang, China, and recorded as Rictularia cristata Froelich, 1802, was investigated. Five large gravid females, more than 40 mm long, eight small (circa 2 mm long) immature females shortly after final molt, and two males, were studied. The males showed prominent dimorphism: one had the typical male form with a ventrally-curved tail and developed precloacal fan-like crests; the other resembled a small female in its external morphology and lacked precloacal crests. Identity of the Rictularia species parasitic in Apodemus spp. of the Far East with R. cristata in Europe should be examined using DNA sequence analysis.中国藩陽産セスジネズミApodemus agrariusから得られ, Rictularia cristata Froelich, 1802として報告されたリクチュラリア科線虫の形態を記載した。体長40mm以上の大型の成熟雌成虫5個体,体長約2mmの最終脱皮直後の未熟小型雌成虫8個体および雄成虫2個体について検鏡, 計測を行った。雄虫は顕著な二型を示した: 一方は腹側に曲がった尾部と発達した総排泄腔前の扇状突起を有する典型的な雄型,他方は総排泄腔前の突起を欠き,外見的に小型の雌に類似していた。極東地域のアカネズミ属に寄生するRictulariaがヨーロッパのR. cristata と同一種かどうかは,DNA塩基配列の解析によって検討されるべきである。journal articl

    Localized expression of the Dwarf14-like2a gene in rice roots on infection of arbuscular mycorrhizal fungus and hydrolysis of rac-GR24 by the encoded protein

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    ArticleStrigolactones (SLs) are plant hormones that control diverse aspects of the shoot and root growth and are exuded into the soil as recruitment signals for arbuscular mycorrhizal (AM) fungi. SL signaling in plants is transduced via the α/β-hydrolase receptor Dwarf14 (D14). The D14 family consists of D14, Dwarf14-like (D14L), and Dwarf14-like 2 (D14L2) clades in rice. The D14L receptor is known to condition pre-symbiotic perception of AM fungi. In this study, it was found that the Dwarf14-like2a (D14L2a) gene expression was significantly induced by AM fungal colonization. The transcript of D14L2a appeared not only in mature arbuscule-containing cells but also in epidermal/cortical cells at an early colonization stage and near the elongating intercellular hyphae. D14L2a transcript was detected normally in mycorrhizal roots of str1-2 mutant that form stunted arbuscules, suggesting that the gene expression is independent of arbuscule development. Moreover, the recombinant D14L2a protein exhibited hydrolase activity of synthetic SL, rac-GR24. Based on these results, we discussed the role of D14L2 in the establishment of AM symbiosis.journal articl

    Comparison of distal forelimb conformations between Japanese Black and Holstein-Friesian newborn calves

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    ArticleFlexural and hyperextension deformities are congenital problems in calves. We, therefore, aimed to investigate the distal limb conformation in 1 day- and 28-day-old female Holstein-Friesian (HF) calves (n=21), male Japanese Black (JB) calves (n=15), and female JB calves (n=15). The claw angle of the forelimb dorsal claw wall in a standing position and recorded other parameters, including body weight, withers height, circumference of forelimbs, and flexor tendon thickness in the forelimbs, were measured and compared these between the three groups. At 1 day old, the mean claw angles were 51.1° in female HF calves, 47.0° in male JB calves, and 41.8° in female JB calves; the 95% confidence intervals (CIs) of the claw angles showed large distributions in all three groups. One female HF and one male JB calves showed mild flexural deformity, whereas four JB calves showed hyperextension deformity. At 28 days old, the mean claw angles were 51.7° in female HF calves, 51.2° in male JB calves, and 48.4° in female JB calves; the 95% CIs of the claw angles showed smaller distributions than those at 1 day old in all groups. For all groups, the limb deformities had improved without treatment at 28 days old. As a feature of the breed, female JB calves were apt to show hyperextended deformities inversely proportional to the body weight. These limb deformities healed spontaneously and were thought to be physiological.journal articl

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