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イヌの Phase–Contrast Magnetic Resonance Imaging（PC-MRI）による頭蓋内評価の基礎研究と臨床応用
Get PDF酪農学園大学獣医学Thesisdoctoral thesi
書評『獣医学を学ぶ君たちへ : 人と動物の健康を守る』 : 中山裕之著, 東京大学出版会, 2019年5月刊, 定価2,800円+税, A5判168頁
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書籍紹介 : 『魚は痛みを感じるか？』ヴィクトリア・ブレイスウェイト著 高橋洋訳 : 2012年2月 紀伊國屋書店発行 259頁 定価 (本体2,000円+税)
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書評 『里山に暮らすアナグマたち : フィールドワーカーと野生動物』金子弥生 [著] (東京大学出版会，2020年11月，248頁，3,800円＋税)
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Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis
Get PDFArticleMycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. bovis. However, even though neutrophils accumulate in M. bovis infection, the interaction of M. bovis and neutrophils has not been fully elucidated. We attempted to elucidate the innate immune response of neutrophils stimulated with M. bovis and evaluate the transcriptome and functional analysis of bovine neutrophils stimulated with M. bovis. Proinflammatory cytokines, such as inducible nitric oxide (iNOS), which was the most increased gene in transcriptome analysis, were increased in quantitative polymerase chain reaction analysis of bovine neutrophils stimulated with live or heat-killed M. bovis. Nitric oxide and intracellular reactive oxygen species production of neutrophils stimulated with M. bovis was significantly increased. Neutrophils stimulated with M. bovis showed an increased ratio of nonapoptotic cell death compared to unstimulated controls. We demonstrated that neutrophil extracellular traps (NETs) formation was not recognized in neutrophils stimulated with live M. bovis. However, heat-killed M. bovis induced NETs formation. We also showed the interaction with M. bovis and bovine neutrophils regarding proinflammatory cytokine gene expression and functional expression related to NETs formation. Live and killed M. bovis induced innate immune responses in neutrophils and had the potential to induce NETs formation, but live M. bovis escaped NETs.journal articl