İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
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Development of an HPLC method for the determination of fampridine (dalfampridine) in human plasma by fluorimetric derivatization and application to a prototype pharmacokinetic study
No full textFampridine (dalfampridine) is used to improve walking in people who have multiple sclerosis. In this study, a new, fast and sensitive HPLC method with fluorometric detection was developed for the determination of fampridine in human plasma and applied to a pharmacokinetic study. Fampridine was precolumn derivatized with 7-chloro-4-nitrobenzofurazan (NBD-Cl) and the fluorescent derivative was separated on a C18 (150 × 4.6 mm × 2.6 μm) analytical column at 30 ºC using a mobile phase composed of acetonitrile – 0.1% o-phosphoric acid in water (80:20, v/v) by isocratic elution with flow rate of 1.0 mL min–1 . The method was based on the measurement of the derivative using fluorescence detection (λex= 310 nm, λem = 365 nm). The retention time of fampridine is 4.10 ± 0.02 min. This currently developed method was validated according to EMA criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 0.01–20 ng mL–1 with the correlation coefficient of 0.9996. LOD and LOQ were found to be 0.003 and 0.01 ng mL–1 , respectively. Intraday and interday RSD values were less than 2.45%. The plasma concentration-time profile and pharmacokinetic parameters such as AUC0–t, AUC0–∞, Cmax, tmax, t1/2, were calculated according to the assays. The presented method can certainly be used for bioequivalence and bioavailability investigations and routine analysis of the drug in plasma
Fibromiyalji tedavisinde hasta eğitiminin etkisi: Randomize kontrollü çalışma
No full textObjective: Fibromyalgia syndrome (FMS) can be treated with a variety of pharmaceutical and non-pharmacological approaches. The purpose of this study was to evaluate the effects of patient education and a home exercise program on FMS patients' pain, fatigue, functioning, and quality of life. Materials and Methods: The study included patients who satisfied the American College of Rheumatology's FMS criteria. They were split up into two groups at random. A schedule of at-home exercise was given to both groups. Furthermore, Group 2 patients participated in patient education sessions. At baseline and eight weeks later, both groups underwent examinations. Evaluation criteria included the Nottingham Health Profile (NHP) for quality of life, the Beck Depression Inventory (BDI) for psychological status, the Visual Analogue Scale for pain severity (VASp) and exhaustion (VASf), and the Fibromyalgia Impact Questionnaire (FIQ) for functional status. Results: The primary clinical and demographic characteristics of the two groups were comparable at baseline. Although the values of VASp (p=0.014), VASf (p=0.011), BDI (p=0.043), NHP subscales (p<0.05), FIQ (p=0.012) showed statistically significant improvements in Group 2, only Group 1's FIQ score significantly improved (p=0.027). There were substantial differences in VASp (p=0.026), VASf (p<0.001), BDI (p<0.001), and all NHP subscales except sleep problems, FIQ scores (p=0.036) between the two groups in favor of Group 2 at the end of eight weeks. Conclusion: Patients with FMS should receive patient education in addition to home-based exercise, which is an effective treatment for reducing symptoms, enhancing psychological well-being, and improving quality of life
Investigation of the attitudes of faculties of medicine, dentistry, and pharmacy students towards brain drain in terms of future anxiety and satisfaction with life levels
No full textThe aim of this study is to examine the attitudes of medical, dentistry and pharmacy faculty students towards brain drain in terms of future anxiety and life satisfaction levels. 308 university students enrolled in a foundation university in Istanbul were included in the study. Descriptive statistics, correlation, multiple regression, one-way ANOVA and independent sample t-test analysis were used in the analysis of the data. The regression analysis results showed that hopelessness about the future, future anxiety and life satisfaction were significant predictors of attitudes towards brain drain. According to the independent samples t-test analysis results, it was found that the tendency towards brain drain was higher in male students and students in the clinical education process, while the tendency towards brain drain was lower in dentistry students than in medical and pharmacy students. The study results provide important data for the development of policies to reduce brain drain in the health sector in Türkiye.This research was supported within the scope of TUBITAK 2209-A student project with project number 1919B012322632.
Bu araştırma TÜBİTAK 2209-A öğrenci projesi kapsamında 1919B012322632 proje numarasıyla desteklenmiştir
Role of ovarian stem cells in postnatal oogenesis and implications in fertility preservation
No full textIt is a long-held dogma in reproductive biology that females are born with a set nonrenewable number of oocytes in the ovary. While some animal studies challenged this dogma by demonstrating allegedly postnatal oogenesis and the presence of certain stem cell factor expression germ cell markers, their biological roles are yet to be defined. In this chapter, we revisited this issue revisit this dogma in light of most recent data. The chapter outlines the characteristics of these putative OSCs, including their morphological features, expression of germline markers (e.g., DDX4, DAZL), telomerase activity, and methods for their isolation and culture, acknowledging the ongoing debate and methodological controversies surrounding their identification, particularly the reliability of DDX4-based sorting. We critically evaluate the evidence for their capacity to differentiate into oocyte-like cells in vitro and in vivo, including experiments involving transplantation into animal models. Finally, the chapter explores the profound implications these OSCs hold for fertility preservation, especially for women facing premature ovarian insufficiency, age-related infertility, or gonadotoxic treatments. We discuss potential future applications, such as autologous OSC transplantation to restore ovarian function or in vitro generation of mature oocytes for fertilization. We highlight the significant technical and safety hurdles that must be overcome, including optimizing culture systems, ensuring genomic stability, and validating human functional competency. While the physiological role and definitive existence of functional OSCs in adult human ovaries remain contentious subjects requiring further rigorous investigation using advanced techniques like single-cell analysis and lineage tracing, the potential therapeutic promise continues to drive research in this exciting, albeit controversial, area of reproductive medicine
Hepatoprotective potential of urtica dioica
No full textLiver diseases represent a significant global health burden, a ecting millions of people worldwide and contributing substantially to morbidity and mortality (1). e spectrum of hepatic disorders ranges from acute drug-induced liver injury to chronic conditions such as non-alcoholic fatty liver disease (NAFLD), viral hepatitis, cirrhosis, and hepatocellular carcinoma (2). Despite advances in conventional pharmacotherapy, many existing treatments are associated with limited e#cacy, adverse e ects, and high costs, prompting increased interest in comple mentary and alternative therapeutic approaches (3)
Considering its mechanism of action it may be logical to choose letrozole as an initial option for endometrium preparation before vitrified-warmed embryo transfer
No full textAim: To assess the efficacy of letrozole and programmed hormone replacement therapy (HRT) cycles in terms of obstetric outcomes for women undergoing a single vitrified-warmed blastocyst embryo transfer (SVBT). Methods: This study is a retrospective study conducted in a private IVF (in vitro fertilization) clinic. A total of 324 FET cycles (letrozole group = 183, HRT = 141), consisting of primary infertile patients aged <40 years, were enrolled. The cycle characteristics, pregnancy results, and perinatal parameters were recorded. The primary outcomes of this study are pregnancy and miscarriage rates, live birth rates, whereas hypertensive disease of pregnancy (HDP), intrauterine growth restriction (IUGR) and preterm birth are the secondary outcomes. Results: Positive β-HCG rates were 53% (97/183) versus 61% (86/141), whereas clinical pregnancy rates were 47% (86/183) versus 51.1% (72/141) for the letrozole and HRT groups, respectively. For clinical miscarriage, the results were 6.5% (12/183) for letrozole versus 15.6% (22/141) for HRT groups, which were statistically significant. For live birth rate (LBR), letrozole was found to be slightly better compared to HRT 40.4% (74/183) versus 35.5% (50/141). Considering the perinatal outcomes, the parameters were comparable between the groups. Conclusions: In terms of perinatal outcomes and IVF success, letrozole was not found to be inferior to HRT. Letrozole can be utilized as a safe and effective agent, even as a first-line treatment, given the potential benefits offered by its mechanism of action and being a reasonable option for both ovulatory and unovulatory patients
Trace determination of cobalt in heather leaf tea by matrix matching calibration assisted flame atomic absorption spectrometry following a dispersive liquid–liquid microextraction utilizing a Schiff base ligand
No full textIn this study, an analytical method that involves dispersive liquid–liquid microextraction for extraction and enrichment of Co was developed for its determination in heather leaf tea infusions. N,N’-bis(2-hydrox yacetophenylidene)-1,3-propanediamine was utilized to form the complex extracted in chloroform with meth anol as a dispersive solvent. The experimental parameters were optimized to allow for an enhancement of detection power by 35.6-folds compared to direct FAAS measurements while lowering the limit of detection to 0.0154 mg/L and the limit of quantification to 0.0513 mg/L and providing a linear range of 0.050–0.75 mg/L. Spiked recovery experiments were conducted on two brands of heather leaf tea where a matrix matching strategy was employed for further reduction of interferences. The recovery results obtained in the range of 78.7–128.4 % supported the accuracy of the proposed method that promises an economical and easy-to-apply process for the trace determination of cobalt in herbal tea matrices
Türkiye'de hepatit c'nin ortadan kaldırılması
No full textThis manuscript examines the epidemiological patterns, transmission routes, and genotypic distribution of hepatitis C virus (HCV) in Türkiye, highlighting national progress toward elimination targets set by the World Health Organization (WHO). The main objective is to evaluate the effectiveness of national screening, diagnostic, and treatment strategies, with a focus on the scale-up of direct-acting antiviral (DAA) therapies and their associated cost-effectiveness. Drawing on recent multicenter and population-based studies, the paper outlines the shifting prevalence of HCV geno types, particularly among high-risk populations such as people who inject drugs (PWID), prisoners, and individuals undergoing hemodialysis. The analysis demon strates that genotype 1b remains predominant, though genotype diversity is increas ing due to migration and changing transmission dynamics. Findings reveal that despite improved availability of DAA treatments and health policy initiatives like the 2018–2023 National Viral Hepatitis Program, gaps persist in diagnostic follow-up and referral. The manuscript emphasizes the dual approach of micro- and macro elimination, advocating for integrated care models, increased physician engagement, and enhanced awareness efforts. Projections suggest that achieving WHO goals is feasible in Türkiye if testing and treatment rates significantly improve. Ultimately, this study underscores the necessity of sustained political commitment, intersectoral collaboration, and targeted public-health interventions to reduce HCV-related mor bidity and mortality by 2030
Planetary research: A new diamond open access journal for planetary science
No full textPlanetary Research is a new diamond open-access journal for the planetary sciences and is set to be launched in January 2026. Planetary Research will follow an alternative to the traditional model of commercial publishing: the diamond open access model, whereby the journal is run entirely by volunteers using free and open-source software, and owned by the community, through a non-profit association that has been set up in France (The Planetary Research Cooperative). This means that all articles published in Planetary Research will be available for free for both authors and readers, with no access, subscription, or submission processing charges, whilst the journal scope and principles are determined by the community through participation in the online forum and monthly meetings. Further opportunities for participation include open calls for the positions of editor-in chief, editors, associate editors, and members of the media team and technical team, with deadlines on July the 1st, 2025 (see https://planetary-research.org for details)
Travma sonucu alt süt kesici ve kanin dişlerde görülen sublüksasyon
No full textAMAÇ: Süt dentisyon döneminde dentoalveolar travmalar; düşme, kavga, oyun veya sportif aktivitelerde meydana gelen kaza ve yaralanmalar nedeniyle sıklıkla meydana gelmektedir. Lüksasyon yaralanmaları, süt dentisyon döneminde en sık görülen dental travma çeşitlerinden biridir. Süt dişi travmaları ile oluşan şiddetli yaralanmalar sonrasında sürekli dişlerde çeşitli sekeller meydana gelebilmektedir. Süt dişi travmalarında doğru tanı, zamanında tedavi ve düzenli takip, dişin prognozunu doğrudan etkileyen belirleyici faktörler olup, bu sürecin etkin ve sistematik şekilde yönetilmesi klinik başarı açısından kritik öneme sahiptir. Bu vaka raporunda, dental travma sonucu alt süt kesici ve kanin dişlerde görülen sublüksasyon yaralanmasındaki tedavi yaklaşımı ve takibi bildirilmektedir. OLGU: 4,5 yaşında, sistemik olarak sağlıklı erkek hasta kliniğimize dental travmadan hemen sonra başvurmuştur. Alınan anamnezde, çocuğun okulda alt çenesine tekme atıldığı ve ağzını kapattığında etkilenen bölgedeki dişlerinde ağrısı olduğu bildirilmiştir. Klinik muayene sonrası alt süt kesici ve kanin dişlerin lingual diş eti oluğunda hemoroji ve bu dişlerde hafif mobilite saptanmıştır. Alınan panoramik radyografi sonrası herhangi bir kök kırığı ya da mandibulada eklem kırığı saptanmamıştır. İlk seansta sekonder travmayı önlemek adına oklüzyon yükseltme planlanmıştır. İzolasyon sağlanamadığı için 75 ve 85 numaralı dişlerin oklüzal yüzeylerine yüksek viskoziteli cam iyonomer siman (EQUIA Forte Fil) uygulanarak kesici dişlerin teması engellenmiştir. Antibiyotik (amoksisilin grubu) reçete edilmiştir. Hastanın ailesi beslenme ve fırçalama konusunda bilgilendirilmiştir. Hasta 10 gün sonra kontrole çağrılmış ve travmadan etkilenen dişlerin mobilite-ağrı gibi semptomları olmadığının görülmesi üzerine, cam iyonomer siman kaldırılarak normal oklüzal kontaklar sağlanmıştır. Hastanın 3. ve 6. aylarda kontrollerinde klinik ve radyografik muayenesi yapılmış ve dişlerin asemptomatik olduğu görülmüştür. SONUÇ: Süt dişi yaralanmaları her an karşılaşılabilecek, sonuçları ise çocukların günlük aktivitelerini etkileyebilecek bir durumdur. Hekim tarafından doğru teşhis, tedavi ve takip süreçlerinin yürütülmesi kadar, ebeveyn ve bakıcıların da yeterli düzeyde bilgilendirilip sürece aktif katılım göstermesi, uzun vadeli başarılı klinik sonuçların elde edilmesinde kritik rol oynamaktadır