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Modified malted process to produce malted flours as biosource of maltogenic enzymes-modified malted flour
No full textExtension PCT PCT/EP2025/070731 18 juillet 2025 2023 – DV 5157DI MDL136 – Déclaration d’invention 13-07-20223 Passée en dépôt de brevet mars 2024 - Modified Malted Process To Produce Malted Flour As Biosource Of Maltogenic Enzymes–Modified Malted Flour A. Le-Bail, A. Velasquez, E.A. Norwood (ONIRIS GEPEA), L. Saulnier (INRAE-BIA
Oral supplementation with Algae extract enhances the humoral response to attenuated PRRSV-1 vaccine
No full textInternational audienc
Nucleation mechanism in the absence and presence of an electric field
No full textInternational audienceThe objective of this article is to investigate the mechanism of the ice nucleation process theoretically by assaying the thermodynamics of the ice nucleus as a small system, and experimentally verified by studying the effect of the static electric field on the ice nucleation process. Using the thermodynamic of small system related to Hill, we predicted that the equilibrium temperature of water and ice nucleus decreases with the reduction of nucleus size. By applying an electrostatic field, the Gibbs free energy of nucleus formation decreases due to this fact that the electrostatic work on the ice nucleus is lower than that for bulk water which it causes a decrease in the critical size of the ice nucleus. The experimental results showed that the nucleation temperature of water increases by applying the electric field with the voltage of 4.5 kV (internal strength of 2.2 × 104 V/m) while the size of ice crystals decreases in the frozen agar gel at the presence of electric field
A Mammalian Tissue Standard Reference Material for Improved Lipidomic and Metabolomic Interoperability
No full textInternational audienceIntroduction: Lipids are a highly diverse class of biomolecules essential for energy storage, membrane structure, and cellular signalling. Their dysregulation is implicated in numerous diseases and pathological states. Recent technological advances have made lipidomic analysis more accessible, enabling its application across a wide range of samples and study designs. However, like metabolomics, lipidomics relies heavily on mass spectrometry, which—due to variations in instrumentation, operating conditions, and scanning modes—inherently lacks reproducibility. This variability complicates cross-laboratory and longitudinal data comparison thus limiting impact of studies.Aims: To address these challenges, reference materials (RMs) are systematically analyzed to provide a common benchmark for data comparison. Although frozen human plasma reference materials (e.g., NIST SRM 1950) are available for plasma analyses, and a liver extract (NIST candidate RM 8462) is currently under evaluation, no such standards yet exist for mammalian or plant tissues (1). This project aims to develop a novel RM for mammalian tissues to support both lipidomic and metabolomic analyses. The RM will enhance data robustness and reproducibility, facilitate instrument validation and method development, and improve metabolite annotation.Materials and Methods: Twelve rats were euthanized, and seven organs (liver, lung, muscle, brain, cerebellum, heart, and intestine) were collected. Tissues were freeze-dried, ground, and homogenized. Partners within the MetaboHub consortium analyzed the lipid and polar metabolite profiles of individual organs as well as a composite extract, using a range of techniques, including LC-MS, GC-MS, SFC-MS, and NMR.Results: This presentation will summarize the preliminary targeted and untargeted lipidomic and metabolomic data obtained for each tissue using different analytical platforms. Additionally, mixtures of lipid extracts from various organs were analyzed to identify the combination offering the greatest lipidome diversity.Conclusions & Perspectives: The detailed characterization of these extracts is nearly complete, though storage stability remains to be evaluated. The mammalian RM has already been integrated into several projects, and ongoing work focuses on optimizing its use and establishing best practices for implementation
Livret des visuels d'interventions - Restitution des résultats du projet ANR SENTINEL
No full textLivret des visuels d'interventions - Webinaire de la restitution des résultats du projet ANR SENTINE
Modelling a single-mode 915 MHz microwave applicator for low-moisture food treatment: comparison between COMSOL Multiphysics ® and CST Studio Suite ®
No full textInternational audienceTwo computational models were developed by using COMSOL Multiphysics® and CST Studio Suite® to simulate the microwave heating process of paprika and to compare the electromagnetic results obtained from each modelling software. The dielectric properties of paprika were initially measured at room temperature using the cavity perturbations technique and then estimated as a function of temperature. Experimental validation was performed in a rectangular waveguide with iris openings of 125 (Configuration 1) and 88 mm (Configuration 2). The impact of variations in both dielectric constant and dielectric loss on temperature prediction was examined, along with the sensitivity of the results to the positioning of probes. Simulated and experimental temperatures were compared at two positions inside the product: the centre (T1) and the bottom (T2). Additionally, the distribution of the electromagnetic field and the microwave reflected power were examined. The results highlight the strong impact of dielectric property variations on local temperature prediction and provide valuable insights for optimizing industrial microwave heating processes for food powders
Injectable and degradable calcium phosphate/silanized hyaluronic acid composite foam for bone defect repair: A study in a Rabbit model
No full textInternational audienceCalcium phosphate cements (CPCs) are widely used as bone substitutes due to their injectability, which makes them well-suited for minimally invasive surgeries. However, their biomaterial-induced biological response often falls short in promoting osteogenesis, primarily due to their limited interconnected macroporosity and inadequate biodegradation rate, which impede cell migration and vascularization. Recent advancements have introduced a dual syringe-mixing technique to create injectable composite foam pastes that result into macroporous composite foams. These foams consist of a mineral cementitious precursor (leading to the formation of a calcium deficient hydroxyapatite cement CDHA), combined with 3 different organic matrices such as silanized hydroxypropyl methylcellulose (SiHPMC), gelatin (GEL), or silanized hyaluronic acid (SiHYA). Although the physico-chemical features of these hybrid (organo-mineral) composite foams are comparable, their respective in vivo performances vary significantly. In the present study involving femoral defects in rabbits, all formulations 49 demonstrated high levels of osteointegration and bone formation after six weeks of implantation. However, their biodegradation rates, influenced by the organic matrix, differed markedly, with CDHA/SiHPMC exhibiting minimal degradation and CDHA/GEL showing near-complete biomaterial resorption. Notably, the CDHA/SiHYA composite foam presented an optimal balance between biodegradation and bone formation, suggesting its potential for effective clinical application in bone defect treatment. We have shown herein that, as in the case of industrial composites, these composites for regenerative medicine, that are the result of a combination in a single mass of materials that differ in terms of their chemical nature, achieve a summation of performance depending of their organic phase, in terms of biological reactivity
Targeting the colony-stimulating factor 1 axis for the treatment of Tenosynovial Giant Cell Tumors
No full textInternational audienceDiffuse and Localized Tenosynovial Giant Cell Tumors (D-/L-TGCT) are two closely related forms of benign tumors of the joint characterized by uncontrolled macrophage infiltration and proliferation. Both forms arise from similar translocation events in synovial fibroblasts involving the Colony Stimulating Factor 1 (CSF1) gene, the major regulator of myeloid cell survival and activity. This review first describes CSF1 gene structure and protein expression as well as signaling through its receptor (CSF1R). Then, genetic alterations observed in TGCT patients are described in depth. The main features are translocations that remove the 3’ regulatory elements of CSF1 messenger RNA, driving its overexpression and the abnormal recruitment of non neoplastic macrophages leading to tumor growth. This review then focuses on the resulting histopathological features characterizing the disease, as well as the available treatments targeting the CSF1 axis. Systemic therapies targeting CSF1 or its receptor are valuable for relapsing or inoperable cases, especially D-TGCT, but their use is still limited by heavy side effects and unresponsive tumors. Overall, our understanding of the pathophysiology of these tumors, the efficacy and limitations of current therapeutic options, and the questions that remain unanswered open new avenues for research and provide opportunities to further improve patient care
Role of textural properties of clay-based microporous materials on their CH4/CO2 separation performances in the context of biogas upgrading
No full textInternational audienceThe separation of CO2 and CH4 in the biogas upgrading context is a crucial step toward increasing the share of renewable bio-methane in natural gas infrastructure. Adsorption-based processes, particularly Vacuum/Pressure Swing Adsorption (V/PSA), are widely used for CO2 removal owing to their energy efficiency and scalability. This study examines the CO2 and CH4 adsorption capacities of various natural and synthetic smectite clay minerals with differing layer charges, exchanged with Na+, Cs+, and TMA+ cations, to assess their potential as selective adsorbents in separation processes. X-ray diffraction analysis revealed systematic shifts in basal spacing under the effect of cation exchange, related to the interlayer spaces expansion. Textural properties, including specific surface area (SBET) and micropore volume (Vμp) were determined using the BET and t-plot models applied to N2 adsorption isotherms at −196 °C. The fraction of high-energy surface sites (HESS) was assessed from high-resolution Ar adsorption isotherm data measured at −186 °C. Equilibrium separation performances were determined from measurements of CO2 and CH4 adsorption capacities and binary mixture selectivities. These data were analysed according to clay structural and textural properties. Experimental results reveal that the non-exchanged synthetic nano-clays demonstrate higher selectivity than natural counterparts, likely due to their larger density of surface defects enhancing CO2 interactions at low pressures. Cs + -exchanged clays exhibit narrow interlayer microporosity, which contributes to improve the CO2 adsorption selectivity while excluding CH4 due to steric effects. In contrast, the TMA+-exchanged forms show significant interlayer expansion, enhancing both CH4 and CO2 uptakes, but degrading adsorption selectivity. A quantitative screening of the clay systems based on the computation of hybrid material metrics suggests that tailoring interlayer spaces thanks to cation exchange may significantly improve separation performances of Vacuum/Pressure Swing Adsorption processes