Dane Badawcze UW University of Warsaw
Not a member yet
442 research outputs found
Sort by
Epigraphy & identity in the Early Byzantine Middle East
The ods file contains tables with data from the database available under the link https://epi-identity-database.uw.edu.pl/ This includes the descriptions of archaeological sites where inscriptions in languages other than Greek were found in the late-antique Levant (Syriac, Christian-Palestinian Aramaic, Jewish-Palestinian Aramaic, Hebrew), descriptions of buildings, in which the inscriptions were found, texts of insciptions, prospographical data on people attested in these inscriptions. The chronological range is the fourth - seventh century CE.</p
The polarization switching in nanoscale with an anisotropic 2D magnetic semiconductor
Here we present a proof-of-concept device demonstrating the feasibility to control the light polarization using the properties of the magnetic 2D materials. The studied structure consists of a diluted magnetic semiconductor quantum well and a thin layer of CrSBr. We show that by application of the external field we can switch the sign of the polarization of the emitted light. The theoretical modelling confirms that such a switching is a direct consequence of a collosal shift of the exciton lines observed in the high energy range of the CrSBr spectra. Owing to this mechanism, the full rotation of the polarization state can be realized in a layer as thin as few hundred nanometers.</p
The directions of spatial development of metropolitan areas in Poland
The “localities” vector layer contains localities from three Polish urban complexes (WMA, LMA and OAA) subjected to typology derived from their urban, demographic, and structural characteristics (Typ and Typ2 columns). The layer's attribute table also includes calculations of the component indicators (VL, PD, UI), which were used to carry out classification by cluster analysis (Cluster column).The “zones” layer shows four urban and peri-urban zones resulting from the combination of certain types of localities.Both layers can be opened in any GIS software (e.g. QGIS, SAGA GIS).</p
Effects of climate and anthropogenic pressures on chemical warfare agent transfer in the Baltic Sea food web
Ecopath w Ecosim raw data from the modelling of CWA bioaccumulation transfer within the Baltic Sea food web. Full dataset will be shared upon email request: mj.czub@uw.edu.plPlease consult the Readme.txt and Modelling_excercise.pdf files for additional information.</p
Maternal/zygotic knockout and ddPCR analysis question the role of activin A in preimplantation mouse embryo development
This dataset consists:File 1 with results of the level of activin A protein in the medium from the culture of maternal knockout (m-KO), maternal-zygotic knockout (mz-KO), and wild-type mouse embryonic fibroblasts.File 2 and 3. Two .exe file with results of in vitro maturation of activin A m-KO oocytes from InhbaKO/LoxP Zp3-Cre females and control oocytes from InhbaLoxP/LoxP females, including: File 2 with the number and percentage of mature MII stage oocytes (MII), oocytes after germinal vesicle breakdown (GVBD) and in the germinal vesicle (GV) stage, and degenerated oocytes, after 16 h of in vitro maturation;File 3 with the number and percentage of normal and abnormal meiotic spindles in oocytes after in vitro maturation.File 4 with data of morphokinetics parameters from time-lapse imaging of activin A m-KO, mz-KO, and control embryos.File 5 with the number and percentage of cell lineages cells of m-KO, mz-KO, and control blastocysts.File 6 with results of mitochondrial activity in m-KO and control MII oocytes, based on the green to red ratio of JC-1 staining. In each experiment, the mean fluorescence intensity of oocytes was normalized to that of control oocytes, which were stained and imaged simultaneously.File 7 with the ddPCR results of the number of Inhba transcripts in wild-type mouse GV, MII oocytes, COCs, and zygotes.Briefly about the project and data acquisition methods:In our project, we aimed to verify whether the lack of a phenotype in Inhba zygotic knockout embryos may be masked by the presence of maternal protein deposited in the oocyte during oogenesis. To this end, we carried out a conditional Inhba knockout in oocytes using Zp3-Cre/LoxP strategy, and received embryos with maternal knockout (m-KO), maternal and zygotic knockout (mz-KO). As a control, we used InhbaLoxP/LoxP embryos with a floxed but functional Inhba gene.The lack of activin A protein in Inhba mz-KO embryos was proved in the ELISA assay of the culture medium from mouse embryonic fibroblasts (MEFs) derived from Inhba mz-KO, Inhba m-KO and wild-type foetuses (File 1). To determine whether maternal activin A depletion affects oocyte in vitro maturation (IVM), we isolated fully grown germinal vesicle (GV) stage m-KO and control oocytes (from InhbaLoxP/LoxP females). After 16 hours of IVM, we calculated the number and percentages of the mature MII stage oocytes (MII), immature oocytes: after germinal vesicle breakdown (GVBD) and in the GV stage, and degenerated oocytes (File 2). We then used chromatin and β-tubulin staining to assess the potential effects of maternal activin A depletion on microtubule organization, spindle poles structure, and chromosome alignment. To visualize all sides, the meiotic spindle structure was assessed based on confocal Z-stack projections (File 3).To compare the phenotype of m-KO and mz-KO embryos with stage-matched control embryos, we analyzed the morphokinetics parameters by recording the progress of their development from the zygote to the blastocyst stage using time-lapse microscopy (File 4). The received blastocysts were immunostained to detect the presence of SOX2, SOX17, and CDX2, markers of epiblast (EPI), primitive endoderm (PE), and trophectoderm (TE), respectively. Then, using Imaris software, we counted the number and percentage of cells contributing to the cell lineages (EPI, PE, and TE) of m-KO, mz-KO, and control blastocysts (File 5).Next, we compared the mitochondrial activity in m-KO and control oocytes based on JC-1 staining. Mitochondrial membrane potential was determined as the ratio of red to green fluorescence intensity, calculated from the sum of signal intensities across all Z-stack layers. In each experiment, the mean fluorescence intensity of oocytes was normalized to that of control oocytes, which were stained and imaged simultaneously (File 6).Next, we used ddPCR analysis to study the Inhba mRNA levels in wild-type mouse GV, MII oocytes, cumulus-oocyte complex (COCs), and zygotes. The number of Inhba transcripts in a single ESC, oocyte, or embryo was calculated as follows: copy number/µl× dilution factor ×200 µl)/(number of ESCs or oocytes or embryos) (File 7).</p
Coprecipitation as a one-step Se separation for determination of isotope ratios completed with revised uncertainty evaluation
Selected geological and biological reference materials were analyzed. These include the United States Geological Survey Reference Materials SGR-1 (oil shale, Green River Formation), SCo-1 (cody shale), MAG-1 (marine mud), European Reference Materials BC210a (selenized wheat flour), and selenium-enriched yeast-certified reference material SELM-1.These SRMs were analyzed: NIST SRM 3149 with the addition of a matrix, artificial seawater, and NASS-4 standard seawater spiked with NIST SRM 3149, SELM-1 and BC210a. In addition, the following geological standards were analyzed: SGR-1, SCo-1 and MAG-1.Selenium isotope ratios were measured at the Biological and Chemical Research Centre of the University of Warsaw using the Plasma 3 multicollector ICP mass spectrometer with 16 Faraday cups (Nu Instruments, Wrexham, U.K.). Three isotopes (77Se, 78Se, and 82Se) were chosen for isotope ratio measurements. Three of the Faraday collectors, H8 (82Se), Ax (78Se) and L2 (77Se), were set to register the signals and mass separation of 0.5 atomic mass unit was applied. All data sets reported in this files were collected between April and July 2023.The HGX-200 advanced membrane hydride generation system was applied for sample introduction to MC instrument. The instrument was running in dry plasma mode. The Time-Resolved Analysis (TRA) mode was used for gathering experimental data.The selenium isotope ratios were determined using the SSB method by sequential measurements of the standard-sample-standard. Such external calibration with NIST SRM 3149 provides the delta value. The results are expressed as δ.The sensitivity we achieved was >1 V 78Se per 100 μg/L. All samples were diluted to total Se content around 100 μg/L, except for the NASS-4 sample which was spiked at level 50 μg/L.</p
Investigating the alteration of membrane properties caused by doxorubicin: application of phospholipid mono- and bilayer biomembrane models.
This study shows the effects of doxorubicin (DOx) on DMPC monolayers formed at the air–water interface and bilayers formed at the gold-solution interface. Monolayer studies were performed usin Langmuir technique supplemented with polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) at the air-water interface. Supported bilayers were visualized by atomic force microscopy (AFM) and characgterized by electrochemical measurements and PM-IRRAS studies of supported bilayers.The dataset contains files (text and tiff files) showing the experimental results included in the publication. Please consult the readme.txt file for additional information and a more detailed description of each txt and tif file.</p
Induced Droplet Ovalisation (IDO): Image-based microfluidic method for high-throughput and label-free characterization of microbial proteolytic strains from wastewater sludge
High-speed camera recordings for image analysis of bacterial colonies encapsulated in gelatine droplets (Dataset for https://www.sciencedirect.com/science/article/pii/S0925400525013309).Detailed description can be found in the attached readme.txt.Aims: To develop a label-free, high-throughput microfluidic method for screening proteolytic bacteria from environmental samples, overcoming the limits of traditional cultivation.Methods: Single cells were encapsulated in gelatine droplets, deformed in a custom microfluidic device, and analyzed via high-speed imaging and AI-based classification.Conclusions: The IDO method distinguished proteolytic from non-proteolytic strains with higher sensitivity, throughput, and recovery than plate assays, offering a cost-efficient and scalable tool for microbial and hydrogel studies.</p
Rapid photoinduced self-healing, controllable drug release, skin adhesion ability, and mechanical stability of hydrogels incorporating linker-modified gold nanoparticles and nanogels
This dataset comes from a study exploring how to improve thermoresponsive poly(N-isopropylacrylamide) (pNIPAm) hydrogels for use in biomedical applications like drug delivery. To boost their performance, we added a custom-made cross-linker derived from natural amino acids called N,N′-bis(acryloyl)cystine (BISS), gold nanoparticles (GNPs) that bond through gold–sulfur interactions, and small nanogels made from the same pNIPAm-BISS material. These additions helped the hydrogels become stronger, heal themselves faster after damage, stick better to skin, and release drugs more effectively. We observed that the mechanical strength improved by about 65%, and the gels could stretch 25% more. Using a near-infrared (NIR) laser (800 nm, 0.75 W), the gels healed up to 85% in just 2 minutes. Drug release was also much better, reaching 0.08 mg/mL—almost double compared to unmodified gels. The data includes results from mechanical tests, drug release experiments, healing tests with NIR light, and temperature response studies. Altogether, these materials show good promise for smart biomedical systems that respond to light and heat.</p
Theoretical uncertainties for primordial black holes from cosmological phase transitions
Data used to plot the figures presented in the paper "Theoretical uncertainties for primordial black holes from cosmological phase transitions", arXiv:2506.15496.The files contain data for Figure 1 in the paper. Uploaded files are four .csv files corresponding to four subplots of Figure 1. The naming convention of .csv files follows the examples:SU2_1 --> SU(2)cSM model, I(Tp) = 1,SU2_34 --> SU(2)cSM model, I(Tp) = 34,i.e., we first denote the gauge group of the dark sector, and then the value of I(Tp) parameter for the percolation criterion. Each file contains data in three columns, with self-explanatory headers.Note that the data here are "raw," and to recreate the plots visible in the article, one should use interpolation and a median filter for the relevant regions of the parameter space.</p