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Geotechnical Innovations for Seismic-Resistant Urban Infrastructure
No full textAs urban areas continue to expand into seismically active regions, the imperative for developing earthquake-resistant infrastructure has never been greater. This paper presents a comprehensive examination of innovative geotechnical engineering solutions aimed at enhancing the resilience of urban infrastructure against seismic threats. It explores cutting-edge approaches including advanced base isolation techniques, soil liquefaction mitigation strategies, and the incorporation of shape memory alloys (SMAs) in foundation systems. Through detailed case studies, such as the Tokyo Skytree, Christchurch's soil stabilization projects, and the San Francisco Bay Bridge retrofit, the effectiveness, adaptability, and sustainability of these solutions are demonstrated. The paper conducts a comparative analysis of these technologies based on cost, implementation feasibility, and seismic mitigation effectiveness, and assesses their sustainability in the context of urban development. Despite facing challenges such as high initial costs and the need for specialized expertise, the potential of these technologies to significantly improve the safety and sustainability of urban environments is clear. The study concludes with a call for continued innovation, interdisciplinary collaboration, and proactive policymaking to foster the widespread adoption of these critical advancements. This research not only contributes to the academic field but also provides practical insights for engineers, urban planners, and policymakers striving to build more resilient cities in the face of increasing seismic risks
Peripartum Ketone and its Relationship with Milk Quality in Dairy Cattle
No full textKetosis is a common metabolic disorder in dairy cows and has been associated with alterations in milk composition and a decrease in milk quality, impacting both the economic and nutritional value of dairy products. The purpose of this study was to evaluate ketone levels before and after calving and their effect on milk quality in cattle in the district of El Mantaro, Jauja in the Peruvian highlands. Multivariate analyses, including Principal Component Analysis (PCA) and clustering, were employed to explain the variability in the data better. The study was conducted at the Instituto de Investigaciones Tropicales y de Altura (IVITA) and El Mantaro experimental stations cattle in the district of El Mantaro, Jauja, using a total of 72 Brown Swiss cattle, all of which received similar management. Blood and milk samples were collected from all cows studied and analyzed in the laboratory for ketone levels and milk quality such as density, non-fat solids, protein, freezing point, solids, and lactose. The descriptive analysis revealed significant variations in the evaluated variables, highlighting a reduction in ketone levels after calving and consistency in milk composition, such as non-fat solids and density. The PCA showed that the first two principal components explained 49.8% of the total variability, dominated by compositional variables, while subsequent components contributed smaller proportions, reaching 100% with 11 components. The reduction in ketone levels after calving suggested metabolic stabilization associated with energy recovery during this stage, while differences in compositional variables such as protein and non-fat solids reflected the influence of factors such as diet, genetics, and physiological status. Although ketones showed weak to moderate correlations with the evaluated variables, the negative relationships with body condition and non-fat solids indicated that better nutritional and metabolic status might be associated with lower ketone levels
The Differential of Glucocorticoid Bioavailability Modulators mRNA Expression and Neurotrophic Factors in Three Organs in Broiler Chicken under Immobilization Stress
No full textThe hypothalamus-pituitary-adrenal (HPA) axis remains active despite the elevated corticosterone (CORT) levels during immobilization stress (IMS). This indicates that the HPA axis activity is dependent not only on CORT concentrations but also on the availability of free active CORT that is unbound of corticosteroid-binding globulin (CBG) and is activated by the 11β-hydroxysteroid dehydrogenase (11β-HSD) enzyme. The study examined the CORT levels in the blood and the mRNA expression of both proopiomelanocortin gene in the anterior pituitary gland (APit) and brain-derived neurotrophic factor (BDNF) in the septohypothalamus during IMS. Additionally, the expression of glucocorticoid receptor (GR), 11β-HSD, and CBG were analyzed in the septohypothalamus, APit, and liver. The experiment included three male Cobb 500 chicken groups, a control group, and two treatment groups exposed to 60 or 120 minutes of IMS. Blood, brain, APit, and liver were collected at 35 days of age (N= 12 samples/group). CORT concentrations in blood were quantified using radioimmunoassay, while reverse transcription-quantitative PCR was used to measure mRNA levels of CBG, 11β-HSD1, 11β-HSD2, and BDNF in the septohypothalamus, APit, and liver. The findings suggested that the IMS activated the HPA axis, as demonstrated by increased CORT levels and changes in proopiomelanocortin expression within the APit of stressed chickens compared to unstressed ones. The septohypothalamus of stressed chickens showed an increase in the CBG, BDNF, and 11β-HSD1 mRNA levels, whereas 11β-HSD2 and GRs expression remained stable compared to the control group. Although CBG and BDNF expression decreased from peak levels, their mRNA remained significantly elevated in the 120-minute group. In liver tissue, the treatment groups showed higher levels of 11β-HSD1 and CBG expression, but 11β-HSD2 expression decreased. Overall, CORT levels and the expression of GR and CORT modulators seemed to have a significant influence on the stress response. Notably, increased mRNA levels of CBG and 11β-HSD1 could improve the availability of free active CORT. Furthermore, a positive correlation between CORT levels and BDNF expression was demonstrated, highlighting the role of BDNF in neuronal protection during IMS. Additionally, the liver may contribute to stress regulation through the functions of CBG and 11β-HSD, which are vital for CORT activation and transport
Performance of Quail (Coturnix Japonica) Fed Diets with Fish Meal Substituted by Catfish Offal Flour (Pangaius hypopthalmus)
No full textCatfish offal flour (COF; Pangaius hypopthalmus) has the potential to replace fish meal (FM) due to its high crude protein content. The present study aimed to investigate the effects of substituting FM with COF in basal diets on food intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) in quails. A total of 100 male quails were randomly assigned to five treatment groups, each with four replicates. The treatment groups were fed with basal diet + 0 COF and 100 FM (T0), basal diet + 25 COF and 75 FM (T1), basal diet + 50 COF and 50 FM (T2), basal diet + 75 COF and 25 FM (T3), and basal diet + 100 COF and 0 FM (T4). Feed intake, BWG, and FCR were measured from 0 to 35 days of age. The findings indicated that substituting FM with COF up to 100 did not significantly affect FI, BWG, and FCR. It can be concluded that COF has the potential to replace FM in basal diets while maintaining performance in quails. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/. © The Author(s) 202
USING LIGNOCELLULOSE FIBER IN FEED TO MAINTAIN HEALTH AND IMPROVE THE GROWTH OF POST-WEAN PIGLETS
No full textThis study aims to determine how adding fiber lignocellulose in feed affects performance, intestinal health, morbidity, and mortality in post-wean piglets. The animal model used was 54 piglets divided into three groups of treatments: the control group was given starter commercial feed, group T1 was given starter pig feed with added 1 lignocellulose fiber, and the T2 group was assigned starter feed with the additional 2 lignocellulose fiber. Treatment is given for 4 weeks starting at 41–68 days old. Health observations were also carried out during the research. Piglet performance is determined based on feed intake, body weight and feed conversion ratio (FCR). Examination of the health of the gastrointestinal tract by measuring the length and weight of the intestine, microscopic length of the villi and depth of the crypts, as well as the number of Escherichia coli in the piglet’s small intestine. The research showed that adding 1 and 2 lignocellulose fiber in the feed for 28 days may increase feed intake and body weight and reduce FCR significantly. Adding lignocellulose fiber may significantly lower diarrhea incidents, and death was not found in the treatment group. Lignocellulose fiber may increase villi’s intestinal length and weight height compared with control. It can be concluded that supplementing lignocellulose fiber through feed may increase the piglet’s health growth and efficiency. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/. © The Author(s) 202
PRINCIPAL BREEDING FACTORS INFLUENCING MILK YIELD AND REPRODUCTION IN RED CHITTAGONG CATTLE
No full textThe study aimed to investigate the principal breeding factors influencing milk yield and reproduction in the Red Chittagong cattle (RCC). Retrospective records of a total of 20 dairy cows from the 1st to the 2nd parities were collected from the record sheet from January 2020 to June 2022. Results indicated that birth weight of the RCC was 7.5 higher (P=0.023) in parity 2 when compared with parity 1. Accordingly, total milk yield per lactation was 10.2 higher (P=0.004) in parity 2 in comparison with parity 1. Age at first service was 34.7 higher (P=0.001) in parity 2 compared with parity 1. Accordingly, age at first conception was 38.3 higher (P=0.001) in parity 2 compared with parity 1. The dry period was 22.8 higher (P=0.001) in parity 1 compared with parity 2. Age at first service was 18.3 higher (P=0.014) in natural service (NS) compared with artificial insemination (AI). Age at first conception was 16.5 higher (P=0.023) in NS compared with AI. Post-partum period was 11.9 higher (P=0.008) in AI compared with NS. Days open was 8.9 higher (P=0.018) in AI compared with NS. Calving interval was 2.9 higher (P=0.006) in AI compared with NS. An increased probability of infertility was associated with NS compared with AI. Birth weight of the calf and dry period of the dam were negatively correlated while live weight and post-partum period of the dam were positively correlated milk yield at the expense of reproductive health. A decreased probability of milk fever, mastitis, metritis and infertility was associated with increased milk yield. Principal component analysis revealed that days open, calving interval and service per conception were the principal eigenvectors determining performance of RCC. Overall, RCC performed better in the 2nd parity compared with the 1st parity. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. © The Author(s) 202
GENOTYPIC CHARACTERIZATION OF Escherichia coli ISOLATED FROM INFECTED CHICKEN IN BASRAH, IRAQ
No full textThis study aimed to detect the presence of Escherichia coli in broiler and layer hens in the Basrah province, Iraq using macroscopic and microscopic diagnosis and bacterial isolation that causes infection in some internal organs (liver and heart), and by polymerase chain reaction. Randomly chosen samples were taken from different places within Basrah province for further investigation (poultry fields in Al-Qurnah and AlHartha). The bacteriological analysis revealed that the presence of Escherichia coli is responsible for causing fibrinous pericarditis and perihepatitis in birds. The macroscopic examination revealed hemorrhagic lesions and a significant buildup consisting of a white fibrous accumulation in the pericardial sac of the infected birds' hearts. The livers of infected birds exhibited significant deposition of white fibrous exudate on the liver surface, along with hepatomegaly. The afflicted heart displays a microscopic appearance marked by a notable aggregation of inflammatory cells in the pericardial sac and the release of fibrinous exudate. Additionally, there is an accumulation of edematous exudate in the cardiac muscle fibers, accompanied with congestion of blood vessels in the myocardium. The microscopic examination of the infected liver revealed the existence of a significant infiltration of inflammatory cells in the liver capsule, as well as the presence of a thick fibrinous exudate encapsulated on the liver surface and congestion of the central vein. The histological analysis of the affected heart and liver revealed a significant buildup of collagen and fibrin fibers, which exhibit a prominent dark bluish staining. This buildup is widely distinguished in the pericardial and hepatic capsules. The study indicated that fibrinous pericarditis and perihepatitis affected birds, as indicated by the examination of bacterial results. Escherichia coli emits endotoxins that induce vascular damage in the heart and liver, resulting in an elevated presence of fibrin exudate around the affected tissue. The histological analysis supported this conclusion. © Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/
Heritability and Genetic Correlations of Carcass and Meat Quality Traits in White and Brown Strains of Japanese Quail
No full textSuccessful breeding programs for Japanese quails rely on accurately estimating genetic parameters linked to economically important traits such as body weight, carcass characteristics, and meat quality. The objective of the present study was to evaluate body weight (BW) characteristics, carcass attributes, and their genetic correlations with select meat quality traits in two strains of Japanese quail (white and brown). A total of 530 quail chicks, with 265 from each strain, were included in the analysis. At six weeks of age, the quails were slaughtered, and carcass traits as well as amino acid profiles were measured. For BW traits, the heritability (h2) estimates ranged from 0.27 at d 1 to 0.36 at d 42. The h2 estimated for carcass traits ranged from 0.19 for liver weight, to 0.42 for carcass yield (CY). The h2 estimated for drip loss (DL) of meat quality was 0.21, and the h2 estimate was 0.35 for the meat’s ultimate Ph (Phu). White quail quails recorded the heaviest weight of all carcass traits. Also, white quails had the highest water-holding capacity (WHC), yellowness (b*), and lightness (L*) with the lowest level of DL, cooking losses (CL), and redness (a*) in muscles compared with brown quails. A high genetic correlation of 0.32 was noted between CW carcass weight (CW) and b*. For the pHU, a negative correlation of -0.11 was exhibited with BW. In contrast, L* appeared to have a positive but smaller relationship with CW and CY. High negative correlations were noted for b* with CY -0.27. The CW showed a moderate relationship (0.19) with CL. In conclusion, the current study revealed that the white quail strain had high BW, as well as the finest carcass traits and meat quality. Therefore, white plumage Japanese quail might be preferred as a meat-producing strain. © The Author(s) 202
Identification and Antibiotic Resistance of Pasteurella multocida Isolated from Infected Layer Chickens in West Java, Indonesia
No full textBacterial infections, such as those caused by Pasteurella multocida serotype A, pose significant threats to poultry farming. The use of antibiotics to treat these infections can lead to antibiotic resistance. The present study aimed to identify Pasteurella multocida from 14 Hisex Brown layer chicken hen farms, with chikens aged 25–55 weeks, in West Java, Indonesia, and to evaluate their resistance to various antibiotics. Three samples from each farm were collected from dead chickens having symptoms of fowl cholera. Initially, the study involved isolating and identifying isolates from liver, heart, and lung organs via polymerase chain reaction. The colony was then tested for antibiotic resistance using the disk diffusion method. The results showed that 13 samples were Pasteurella multocida and nine were serotype A. The test results also indicated that all isolates were resistant to colistin (10 µg) and sensitive to tetracycline (30 µg), amoxicillin (25 µg), enrofloxacin (5 µg), sulfamethoxazole (25 µg), lincomycin (109 µg), and ciprofloxacin (5 µg). The study concluded that none of the Pasteurella multocida type A isolates were any longer sensitive to colistin, with some isolates still sensitive to tetracycline, amoxicillin, enrofloxacin, sulfamethoxazole, lincomycin, and ciprofloxacin, and two isolates showing multidrug resistance patterns. © The Author(s) 202
Evaluation of Salmonella Enteritidis Isolated from Layer Hens and Murine Fecal Pellets in Poultry Farms of Libya
No full textThe rodents play a significant role in the transmission of Salmonella between farms and regions. The present study aimed to compare the virulence of Salmonella enteritidis isolated from fecal samples of laying hens and murine within the same poultry house but different regions in Libya using Vivo-quantitative measurement of invasiveness (chicken intestinal loop model). A total of 540 cloacal swabs from laying hens (Hy-line brown chickens) aged 36 weeks and 200 batches of murine fecal pellets were collected from the same poultry house at Gaser Bin Gisher and Furnag regions in Libya. The samples were passed on pre-enrichment broth (Buffered Peptone Water) and enrichment broths (Rappaport Vassiliadis, Selenite broth, and tetrathionate), then the samples were cultured onto Xylose Lysine Deoxycholate agar, brilliant green agar, Salmonella Shigella agar, and Hektoen enteric agar. Single colonies were selected and stained by gram stain and tested biochemically using analytical profile index (API) 20 tests. Salmonella enteritidis was isolated from all the collected samples. The invasion of Salmonella enteritidis isolated from laying hens and murine feces was significantly higher in the anterior inoculation position compared to the posterior position of jejunum in both regions. The account of Salmonella enteritidis isolated from laying feces of hens and murine at Gaser Bengasher region was significantly higher than that isolated from the AlFurnge region. In the present study, the rodents act only as mechanical transmitters without affecting Salmonella invasiveness capacity. Furthermore, the invasion of Salmonella enteritidis depends on the inoculation position in the jejunum. Moreover, the invasiveness variation of Salmonella enteritidis isolated from the Gaser Bengasher and AlFurnge regions could be attributed to the presence of different Salmonella strains in the studied area. Salmonella enteritidis isolated from poultry and murine in the current study was sensitive to gentamicin, ciprofloxacin, and enrofloxacin and resistant to doxycycline, chloramphenicol, sulfafurazol, and ampicillin. © Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/