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Comparing the malignant properties of parental and a knock-in version of HCT116 cell line expressing the CDK2-mutant of eukaryotic elongation factor 2 (eEF2)
No full textBackgroundModulation of protein synthesis according to the physiological cues is maintained through tight control of Eukaryotic Elongation Factor 2 (eEF2), whose unique translocase activity is essential for cell viability. Phosphorylation of eEF2 at its Thr56 residue inactivates this function in translation. In our previous study we reported a novel mode of post-translational modification that promotes higher efficiency in T56 phosphorylation. Cyclin A/CDK2-mediated phosphorylation of eEF2 at the S595 residue is required for more potent phosphorylation at the Thr56, suggesting CDK2 takes a role in robust suppression of protein synthesis.Methods and resultsIn the current study, we analyzed the cell cycle, proliferation, cell death, migration, colony formation, autophagy, and response to Cisplatin properties of the point-mutant variant of HCT116 cells that express the CDK2 mutant (S595A-eEF2) of eEF2. The knocked in S595A mutation resulted in decreased levels of T56 phosphorylation of eEF2, which appears to have similar biological consequences to other experimental manipulations such as silencing the activity of the kinase for the Thr56 residue, eEF2 Kinase (eEF2K).ConclusionOur findings indicate that interfering with the inhibition of eEF2 results in elevated protein synthesis in HCT116 cells and is associated with the progression of malignancy in the colorectal cancer cell line, where eEF2K activity could provide a tumor suppressive role
Investigating the Relationship Between Nitric Oxide Activity in the Brain and Reproductive Organs During the Estrous Cycle
No full textMeeting Abstract PC-8
miR-21 and cathepsin B in familial Mediterranean fever: novel findings regarding their impact on disease severity
Full text linkObjective The limited predictive effect of genotype on familial Mediterranean fever (FMF) phenotype suggests that epigenetic factors and alternative mechanisms that may cause IL-1 beta release could contribute to phenotypic heterogeneity. The objective of this study was to examine the role of IL-1 beta levels and miR-21-5p, cathepsin B and pyrin levels, which were identified as potential factors causing IL-1 beta release through the use of bioinformatics tools, in the pathogenesis of FMF and their relationship with disease severity. Materials and methods 50 paediatric patients with FMF and 40 healthy children were enrolled in this study. Patients were divided into subgroups according to Pras disease severity score. Serum miR-21-5p expression levels were assessed by qRT-PCR, while serum pyrin, IL-1 beta and cathepsin B levels were determined by ELISA. Results Serum miR-21-5p was significantly downregulated in FMF patients compared with the control group (p<0.001), while serum pyrin, IL-1 beta and cathepsin B levels were markedly elevated (p<0.001 for each). Only miR-21-5p was negatively correlated with IL-1 beta (r=-0.855; p<0.001). In moderately severe FMF patients, miR-21-5p exhibited a statistically significant downregulation (p<0.001), whereas IL-1 beta and cathepsin B showed a statistically significant increase (p<0.001 and p<0.05, respectively). Furthermore, the Pras score showed a strong negative correlation (r=-0.738; p<0.001) with miR-21-5p levels. Multivariate logistic regression showed that in FMF, a one-unit decrease in miR-21 increased disease severity risk 6.76-fold, while a one-unit increase in cathepsin B raised it 1.71-fold. Conclusion This might be considered one of the mechanisms for subclinical inflammation in paediatric FMF patients through increased activation of cytokines via the downregulation of miR-21-5p. Our findings suggest that miR-21-5p and IL-1 beta play key roles in subclinical inflammation, and these molecules might be a potential therapeutic target
An Innovative Performance Assessment Method for Increasing the Efficiency of AODV Routing Protocol in VANETs Through Colored Timed Petri Nets
No full textRouting protocols are pivotal in Vehicular Ad hoc Networks (VANETs), serving as the backbone for efficient routing discovery, particularly within the realm of Intelligent Transportation Systems (ITS). However, ensuring their seamless functionality within VANET environments necessitates rigorous verification and formal modeling. Colored Timed Petri Nets (CTPNs) stand out as a valuable mathematical and formal method for this purpose. This study shows a new way to describe the Ad hoc On-Demand Distance Vector (AODV) routing system in VANETs using CTPNs. There are nine pages of detailed analysis using this new modeling method, which allows you to examine success across many levels of a hierarchy. This study provides a strong foundation for building and testing the AODV routing system in VANETs, showing how well it functions in real-life situations. It is interesting to see how the results of the CTPN-based model and simulations compare. Notably, the model finds routes in an average of 32 s, while tests show that it takes 56 s. Additionally, the model's overall number of sent and received packets closely matches the results from the exercise. Furthermore, the suggested plan shows a yield of 41%. Strict T-tests indicate that the modeling results are highly reliable
Müzisyenlerde temporal işitsel işlemleme becerilerinin ve odyolojik bulguların konuşma sesinin akustik özelliklerine etkisi The effect of temporal auditory processing skills and audiological findings on acoustic properties of speech sound in musicians
No full textBu araştırma, müzik eğitiminin bireylerin temporal işitsel işlemleme becerileri, akustik ses
üretim parametreleri ve odyolojik eşik düzeyleri üzerindeki etkilerini çok boyutlu olarak
incelemek amacıyla yürütülmüştür. Araştırma, müzik eğitimi almakta olan farklı sınıf
düzeylerindeki bireyler ile müzik eğitimi almamış bireylerin karşılaştırmalı analizine
dayanmaktadır.
Çalışmaya Karabük Üniversitesi Güzel Sanatlar Fakültesi Müzik Eğitimi Anabilim Dalı’nda
öğrenim görmekte olan 20 birinci sınıf öğrencisi (Müzik 1 Grubu), 20 dördüncü sınıf
öğrencisi (Müzik 4 Grubu) ve 25 müzik eğitimi almamış bireyden oluşan kontrol grubu
olmak üzere toplam 65 birey dâhil edilmiştir.
Araştırma kapsamında her bireye temporal işitsel işlemleme becerilerinin değerlendirilmesi
amacıyla Frekans Patern Testi (FPT), Süre Patern Testi (SPT) ve Rastgele Aralık Tespit
Etme Testi (RATET) uygulanmıştır. Bunun yanı sıra, katılımcıların ses parametreleri Praat
yazılımı (v6.3.15) ile analiz edilmiştir. Akustik analizde temel frekans (F0), jitter, shimmer,
HNR (harmonik-gürültü oranı), intensity ve formant frekansları (F1–F2) değerlendirilmiştir.
Ayrıca, katılımcılara saf ses odyometrisi ve yüksek frekans odyometrisi uygulanarak işitme
eşikleri tespit edilmiş ve her bireye otoakustik emisyon (DPOAE) testi ile 1 000 ile 8 000
Hz aralığındaki duyma eşikleri ölçülmüştür.
Elde edilen bulgular doğrultusunda, müzik eğitimi almış bireylerin hem temporal işitsel
işlemleme testlerinde hem de akustik ses parametrelerinde kontrol grubuna kıyasla daha
başarılı sonuçlar sergiledikleri görülmüştür. Müzik 4 Grubu, Rastgele Aralık Tespit Etme
Testinde en düşük eşik değerlerine, FPT ve SPT testlerinde ise en yüksek skor ortalamalarına
sahip olmuştur. Akustik analiz sonuçlarında müzik eğitimi almış bireylerin jitter ve shimmer
oranları daha düşük, HNR ve intensity değerleri ise daha yüksek bulunmuştur. Bu durum,
ses üretiminde istikrar, rezonans kalitesi ve kontrol becerisinin gelişmişliğini ortaya
koymaktadır. Ayrıca kadın bireylerin ses ve işitsel işlemleme performanslarının genel olarak
daha stabil ve tutarlı olduğu tespit edilmiştir.
xiii
Otoakustik emisyon bulguları özellikle Müzik 4 Grubu’nda 4 000–8 000 Hz frekans
aralığında azalma göstermiş; bu durumun, uzun süreli müzik pratiğine bağlı olarak
kokleadaki dış tüylü hücrelerde harabiyet olduğunu düşündürdüğü değerlendirilmiştir.
Çalışmanın sonucunda, müzik eğitiminin yalnızca estetik bir deneyim değil; aynı zamanda
bireyin nörofizyolojik, bilişsel ve motor sistemleri üzerinde çok boyutlu etkiler yaratan
stratejik bir öğrenme aracı olduğu anlaşılmıştır. Araştırma bulguları, müzik eğitiminin dil
gelişimi, ses terapisi, konuşma bozuklukları ve işitsel rehabilitasyon gibi alanlara entegre
edilebilecek potansiyel katkılarını desteklemektedir
Effect of Heat Treatment on Corrosion Resistance of Electroless Duplex Ni-P/Ni-B Coatings
No full textAs-plated and heat-treated Ni-P, Ni-B, and Ni-P/Ni-B coatings (Ni-P as an internal layer) on steel by electroless plating and their morphology, microstructure, and corrosion performance were evaluated in this study. Scanning electron microscopy analysis demonstrated that all coatings are uniform and adhesion between the substrate and coating was good. Ni-P and Ni-B coatings were amorphous-like structures in their as-plated condition, and by applying heat treatment nickel fully crystallized, nickel borides and nickel phosphides were formed. Immersion tests in 10% HCl and 5% H2SO4 solutions and potentiodynamic polarisation measurements in 3.5% NaCl aqueous solution were applied to investigate the corrosion resistance of the coatings. The results demonstrated that all coatings exhibit better corrosion performance than the substrate steel. Applying heat treatment did not change the corrosion resistance of Ni-P coating, conversely, heat treatment had a dominant positive effect on the corrosion performance of Ni-B and a minor effect on Ni-P/Ni-B duplex coatings
Investigation of the effects of sodium fluoride and gabapentin on an experimental epilepsy model created in zebrafish
No full textMeeting Abstract P-44-03
The effects of lipoic acid on rat submandibular salivary gland in valproic acid induced oxidative stress
Full text linkValproic acid (VA), an anticonvulsant drug, has been associated with various toxic effects, primarily through the induction of oxidative stress. This study aimed to investigate the potential protective role of alpha lipoic acid (LA), a potent antioxidant, against VA-induced oxidative damage in rat submandibular salivary glands. Control, LA, VA, and VA+LA are groups. LA was given 1 h prior to VA administration. After 16 days VA injection, the rats were decapitated, and submandibular salivary glands were taken, homogenized, and examined by biochemical analyses. Biochemical analyses showed that submandibular salivary gland glutathione (GSH) level, superoxide dismutase (SOD) and glutathione-Stransferase (GST) activities decreased; malondialdehyde (MDA), sialic acid (SA) and nitric oxide (NO) levels, tissue factor activity increased significantly in the VA group compared to the control group. No significant changes were found in catalase and myeloperoxidase activities. In the VA group, LA administration caused significant increases in GSH and NO levels; decreases in MDA, SA levels and SOD, GST activities. These findings suggest that LA may offer a protective effect against VA-induced oxidative damage in the salivary glands, potentially through its antioxidant properties. This study highlights the therapeutic potential of LA in mitigating oxidative stress and tissue damage induced by VA
Enhancing Electric Vehicle Battery Charging Efficiency Using an Improved Parrot Optimizer and Photovoltaic Systems
Full text linkThere has been a great need for replacing combustion-powered vehicles with electric vehicles (EV), and fully electric cars are meant to replace combustion engine cars. This has led to considerable research into improving the performance of EVs, especially via electric motor voltage control. A wide range of optimization algorithms have been used as traditional approaches, but the dynamic parameters of electric motors, impacted by temperature and different driving cycles, continue to be a problem. This study introduces an improved version of the Parrot Optimizer (IPO) aimed at enhancing voltage regulation in EVs. The algorithm can intelligently adjust certain motor parameters for adaptive management to maintain performance based on different situations. To ensure a stable and sustainable power supply for the powertrain of the EV, a photovoltaic (PV) system is used with energy storage batteries. Such an arrangement seeks to deliver permanent electric energy, a solution to traditional grid electricity reliance. This demonstrates the effectiveness of IPO, with the resultant motor performance remaining optimal despite parameter changes. It is also illustrated that energy production, by integrating PV systems, prevents excessive voltage line drops and thus voltage imbalances. The proposed intelligent controller is verified based on multiple simulations, demonstrating and ensuring significant improvements in EV efficiency and reliability
Intersensory Interaction and Contemporary Art Paradigms in the Light of Synesthesia
Full text linkSynesthesia, which refers to the phenomenon where the stimulation of one sense affects others, enriches the viewer's perception of the art world, particularly in contemporary art. Many contemporary artists use the interplay of senses brought about by synesthesia to enhance the impact of their work on audiences. By pushing the boundaries of perception and allowing viewers to experience their art in new, multisensory ways, artists deepen their overall engagement with their works. A piece of art enhanced through synesthesia draws viewers in, expanding their sensory experience. This study explores the relationship between sensory interaction and artistic expression, focusing on how contemporary art incorporates synesthesia to shape the audience's experience. By examining artists who use sensory stimuli to create intersensory connections, the paper investigates the interaction established with the audience, emphasizing the benefits synesthesia offers to both artists and art lovers. Through a review of literature, the study analyzes how synesthetic elements influence the connection between artwork and viewers, while also looking at how contemporary art broadens sensory experiences and reshapes the interaction between the viewer and the piece. By positioning synesthesia as an active element in contemporary art, the study shows how sensory interaction is central to the functionality of art. Furthermore, it explores how multi-sensory experiences offered to viewers shape their perception and interpretation of art. The study also discusses the artistic significance of synesthesia, focusing on artists who intentionally incorporate it into their work, and examines the place of synesthetic experiences in artistic practice