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Microbial Art Fixation on Agar Plate: MicArt Fixation for Microbiology Teaching
Get PDFArt based science materials engage students for learning all of the unseen microscobic creatures in easy way microbiology field. In this study we aimed to visualize bacterial and fungal species in artistic way. Inoculation of the colourful strains on agar plate as painting the agar with bacteria, provides beautiful round scenes and clear reminiscence related with the species learning for microbe world. For this purpose, we determined the different concentrations and exposure times of formaldehyde, glutaraldehyde, xylene, toluene, ethyl alcohol, epoxy, glycerin and phenol. We aimed to develop a protocol after determining the optimum storage times. As a method, we exposed all chemicals except epoxy to Petri dishes by spraying method for 1, 5, 10 min. As a result, we determined the fixative that provides the best protection is toluene. After 1 min of toluene exposure, we determined that the Petri dish with growth can be preserved for 6 months even under room conditions without disturbing the color and shape of the whole colony morphology. Here, we obtained best fixation process by using toluen, typically 1 min, depending on the species, was most promising for the preservation of art
Simülasyona dayalı doğum yönetimi eğitiminin ebelik öğrencilerinin klinik uygulama tutumlarına ve öz yeterliliğine etkisi / The effect of simulation-based birth management education on midwifery students' clinical practice attitudes and self-efficacy
No full textEbelik eğitiminde simülasyona dayalı eğitim, öğrencilerin klinik uygulama becerilerinin
gelişimi, klinik uygulamaya aktif katılımı ve öğrencilerin özgüven kazanmasını desteklemesi
açısından önemlidir. Farklı doğum yönetimi eğitimi alan öğrencilerin klinik uygulama tutumları
ve öz yeterliliklerinin belirlenip karşılaştırılmasını yapmak amacıyla planlanan bu araştırma
tanımlayıcı-karşılaştırmalı kesitsel tasarımdadır. İki vakıf üniversitesi Ebelik Bölümü
öğrencilerinden Ocak- Mayıs 2024 tarihleri arasında araştırmaya dahil edilme kriterlerine uyan
öğrenciler (n=104) araştırmaya dahil edilmiştir. Doğum yönetiminde simülasyona dayalı eğitim
alan öğrenciler deney grubunu oluştururken, simülasyona dayalı eğitim almayan grup kontrol
grubunu oluşturmaktadır. Deney grubundaki öğrenciler araştırmacının da uygulamalı derslere
katılımı ile 14 haftalık simülasyona dayalı doğum yönetimi dersi alırken; kontrol grubu beceri
laboratuvarında maket ve malzemeler ile eğitim almıştır. Veriler Öğrenci Tanıtıcı Bilgi Formu,
Klinik Uygulama Değerlendirme Formu, Klinik Uygulamalara Yönelik Tutum Ölçeği ve Öz
Etkililik-Yeterlik Ölçeği ile toplanmış, uygun istatistik yöntemi ile değerlendirilmiştir.
Çalışmamızın sonucunda simülasyona dayalı doğum yönetimi alan öğrencilerin klinik
uygulamada kendilerini daha yeterli hissettikleri (t=1.690; p<.001) belirlenmiştir. Klinik
uygulamaya yönelik tutum ve öz etkililik-yeterliliklerinin benzer özellik gösterdiği saptanmıştır
(p>.05). Öğrencilerin klinik uygulamaya yönelik tutumları ve öz yeterlilikleri arasında pozitif
yönlü orta seviyede bir ilişki olduğu bulunmuştur (p<.05)
Long-term Outcomes of a Contemporary Arthroscopic Bankart Repair Technique in Patients With Traumatic Anterior Shoulder Instability: A Minimum 10-Year Follow-up
Get PDFBackground: The long-term results of arthroscopic Bankart repair are poorly reported in the literature and show recurrence rates as high as 37%. However, this high failure rate is based on historical studies of patients with critical defects stabilized using older techniques. Purpose: To evaluate the long-term clinical outcomes of arthroscopic Bankart repair using a contemporary technique with a minimum of 3 suture anchors in patients with traumatic anterior instability and to assess possible risk factors for recurrent instability. Study design: Case series; Level of evidence, 4. Methods: A total of 181 patients (182 shoulders), who underwent arthroscopic Bankart repair because of traumatic anterior instability between 2005 and 2014, were included in this study and evaluated at a minimum follow-up of 10 years. Exclusion criteria were previous shoulder surgery, additional stabilization procedures, use of 20%, off-track Hill-Sachs lesion). The primary outcome was recurrent instability. Secondary outcomes were the Subjective Shoulder Value (SSV), visual analog scale (VAS), Constant score, Western Ontario Shoulder Instability Index (WOSI), and Rowe score as well as sports activity level and return to sports. Risk factors for recurrent instability were analyzed. Results: The overall recurrence rate was 20.9% at a mean follow-up of 13.8 +/- 2.8 years. Age 1 dislocation before surgery. While younger age and a higher number of preoperative dislocations were potential risk factors for recurrence, a shorter interval between the first dislocation and surgery was associated with improved clinical outcomes. Therefore, patients with a high risk of redislocations should be considered for early soft tissue stabilization, while additional procedures such as remplissage should be performed for those with nonmodifiable high-risk factors
Comparison of plantar pressure distribution patterns of patients with ankylosing spondylitis and asymptomatic healthy individuals: a cross-sectional study
No full textBackground Ankylosing Spondylitis (AS) is an inflammatory chronic disease that causes postural changes. As a result of these changes decreased hip flexion, increased knee flexion and plantar flexion of ankle are developed. Aims The aim of the study is to compare plantar pressure distribution results in patients with AS and healthy individuals and to determine the relationship between clinical and functional parameters in patients with AS. Methods Twenty-five patients (median age = 42.2 +/- 2.1 years) with AS and 25 healthy individuals (median age = 44.4 +/- 1.7 years) were included in the study. The maximum foot pressure (FPmax), average pressure, center of pressure angle (CoP), forefoot load (FFL), rearfoot load (RFL), foot progression angle (FPA) and foot angle (FA) were determined by pedobarographic analysis. Spinal mobility was assessed with lumbar flexion, lateral flexion, and tragus-wall distance. Functional status and disease-related symptoms were assessed with BASDAI, BASFI and ASQoL. Results There were no differences between static and dynamic pedobarographic analysis parameters between the AS group and the control group (p > 0.05). Self-rated functional levels parameters, pain, and stiffness were higher in the AS group (p < 0.05). Lumbar flexion (p = 0.187) and tragus-to-wall distance (p = 0.196) were similar in two groups. Self-rated parameters were correlated with pain, stiffness, and lateral flexion (p < 0.05). Lateral flexion correlated with FA and FFL; tragus to wall distance correlated with FPmax and CoP and RFL; lumbar flexion correlated with FA (p < 0.05). Conclusion The results showed that there was no difference plantar pressure distribution but there was a difference in spinal mobility in AS group compared healthy individuals
Altered Foot Progression Angle and Rearfoot Loading in Patellofemoral and Tibiofemoral Osteoarthritis: A Comparative Cross-Sectional Study
No full textBackground: The purpose of this study was to investigate the differences in plantar loading distribution and functional levels between patellofemoral osteoarthritis (PFOA) and tibiofemoral osteoarthritis (TFOA) patients, and to compare them with healthy individuals. Methods: Sixty patients with knee osteoarthritis and 30 age-matched, healthy individuals (control group) were included in the study. The patients were divided into two groups, the PFOA group (n = 31) and the TFOA group (n = 29), based on the Kellgren Lawrence classification system. The maximum foot pressure of the foot (FPmax), forefoot weight ratio, rearfoot weight ratio, total load on foot, and foot progression angle (FPA) were determined by pedobarographic analysis. Functional level was assessed with the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Results: There was a significant difference in FPA (F-(2,F-79) = 22.322, P .05). Conclusions: The results suggest that the FPA and rearfoot loading were altered in patients with PFOA compared to those with TFOA and healthy individuals. Clinicians need to consider the effect of altered foot position and loading on the knee biomechanics in their treatment approach regarding foot orthoses or gait modification in patients with PFOA
Occupational Therapy Using Sensory Integration for Enhancing Occupational Performance in Children with Autism: A Randomized Controlled Trial
No full textChildren with autism often have difficulties in managing their daily lives due to occupational performance difficulties in activities related to their roles. The study aimed to examine the efficacy of occupational therapy using sensory integration on occupational performance in children with autism. Thirty-five children aged 3 to 10 years with autism were randomly assigned to an intervention group (n = 16) or a control group (n = 19). The intervention group received occupational therapy using sensory integration combined with an evidence-based Home Program, while the control group received only the Home Program. Both interventions were delivered once weekly for 16 sessions over four months. Occupational performance was assessed through Canadian Occupational Performance Measure, with individualized goals identified through Goal Attainment Scaling. The goals identified via Canadian Occupational Performance Measure were interpreted in the context of sensory processing patterns and emotional-behavioral responses assessed by the Sensory Profile, providing a data-driven approach to tailor intervention goals. The intervention group demonstrated significantly higher scores in assessing occupational performance (p =.036) and satisfaction (p =.034). The intervention group also achieved the highest + 1 score in Goal Attainment Scaling (n = 5, 31.3%). therapy using sensory integration, combined with a structured Home Program, effectively enhances occupational performance and goal attainment in children with autism, highlighting the value of child-centered, data-driven interventions.Trial registration Clinical trial number (NCT05718362; Date of registration: 2023-02-08)
Temporal lob epilepsili hastalarda sosyal kognisyonun video temelli gerçek yaşam senaryosu ile incelenmesi: pilot bulgular
Get PDFBu araştırma ile Temporal Lob Epilepsili Bireylerin sosyal kognisyon düzeylerinde herhangi bir bozulma olup olmadığını ve yaşam kalitesi düzeyinin analizi amaçlanmaktadır. Araştırma Atlas Üniversitesi Medicine Hastanesinden Dr. Öğr.Üyesi Demet Aygün ÜSTEL tarafından takipli ve Temporal Lob Epilepsisi tanısı almış 17 olgu ve yaş, cinsiyet, eğitim düzeyi eşleşen 17 kontrol grubu tarafından oluşmaktadır. Çalışmaya yaş ortalaması 37.5 olan 34 katılımcı dahil edilmiştir. Katılımcıların 17'sini kadın (%50) 17'ini (%50) erkek oluşturmaktadır. Katılımcıların 10 tanesi (%29,41) lisans mezunu, 22 tanesi (%64,70) lise mezunu ve 2 tanesi (%5,88) ortaokul mezunudur. Temporal lob epilepsisi bireylerin sosyal kognitif işlevlerinde bozulmalara yol açarak empati yetenekleri, çıkarım yapma becerileri ve kognisyonda bozulmalara sebep olur. Bu bozulmalar dil becerilerine yansır. Dil becerilerinde bozulmaları değerlendirebilmek için TLE'li bireylerle sağlıklı bireylerin içsel durum terimlerini ne sıklıkla kullandığı karşılaştırılmıştır. Bireylere daha önceden hazırlanan gelin, görümce ve damat temalı Gerçek Yaşam Senaryo videosu izletilip buna yönelik sorular sorup verilen cevaplar transkript edilmiştir. Transkript sonuçlarına göre TLE'li bireyler ile sağlıklı bireylerin sosyal kognisyon sorularına verilen cevaplardaki kognitif terim sayısı değerleri arasında ve emosyonel terim sayıları arasında anlamlı bir farklılık bulunmaktadır. (p=0,0050,05) Temporal lob epilepsili bireylerde kognisyonun etkilenip etkilenmediğini incelemek içinse Addenbrook Kognitif Bataryası uygulanmıştır. Temporal lob epilepsili bireylerle sağlıklı bireylerin Addenbrook skorları karşılaştırıldığında anlamlı bir farklılık bulunmuştur. (p=0,0010,05) Addenbrook Cognitive Battery was applied to examine whether cognition was affected in individuals with temporal lobe epilepsy. A significant difference was found when Addenbrook scores of individuals with temporal lobe epilepsy and healthy individuals were compared (p=0.001<0.05). According to the results given in the transcribed answers, it was observed that TLE individuals produced fewer cognitive terms and emotional terms than healthy individuals. The mean number of metaphors was very close to each other and no significant difference was found. In accordance with the studies in the literature, it was observed that cognition and consequently affective and cognitive empathy were impaired in individuals with TLE. Keywords: Epilepsy, Temporal Lobe, Social Cognition, Language, Affective Empathy, Cognitive Empath
Synaptophysin-like-1: A Novel Serum Diagnostic Marker for Pancreatic Ductal Adenocarcinoma Screening, Early Diagnosis, and Prognosis Prediction
Get PDFBackground: The role and underlying mechanisms of synaptophysin-like-1 (SYPL1), a neuroendocrine-associated protein, in pancreatic ductal adenocarcinoma (PDAC) remain unclear. This study aims to assess the diagnostic potential of SYPL1 as a serum biomarker for both resectable PDAC (rPDAC) and metastatic PDAC (mPDAC) located at the head of the pancreas. Additionally, the SYPL1 levels were monitored in PDAC patients who underwent surgical resection, with follow-up measurements taken 6 months postoperatively. Method: We analyzed serum SYPL1 in healthy controls (n = 67), rPDAC patients (n = 39), mPDAC patients (n = 22), and rPDAC patients (6-month postoperative) (n = 20) (due to factors such as relocation or death, 20 patients were included instead of 39 patients) by ELISA. Results: The SYPL-1 levels showed significant differences across the groups (controls: 7.43 +/- 3.32, PC: 15.89 +/- 2.00, mPDAC: 20.01 +/- 4.03, p < 0.001). Both carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) were significantly greater in cancer groups compared to the healthy group. In patients who underwent surgical resection, the SYPL-1 levels showed a significant decrease 6 months after surgery (p < 0.001). Strong correlations were observed between tumor markers, with CA19-9 showing a positive correlation with CEA in both rPDAC (r = 0.550, p < 0.001) and mPDAC (r = 0.623, p = 0.002), while SYPL-1 demonstrated a negative correlation with CEA (r = -0.530, p = 0.009) in mPDAC. Receiver operating characteristic (ROC) analysis revealed excellent diagnostic performance for SYPL-1 in distinguishing both rPDAC (AUC = 0.965) and mPDAC (AUC = 0.985) from healthy controls, achieving superior accuracy compared to conventional markers CEA and CA19-9. Conclusions: Serum SYPL-1 emerges as a promising biomarker for the diagnosis and monitoring of rPDAC and mPDAC. Its significantly elevated levels in cancer groups, coupled with its marked decrease following surgical resection, suggest that SYPL-1 could play a critical role in both initial diagnosis and post-treatment surveillance. The strong correlations observed between SYPL-1, CEA, and CA19-9 further support its potential utility in a multi-marker panel. Notably, SYPL-1 demonstrated superior diagnostic accuracy compared to conventional markers, with high AUC values indicating its excellent ability to distinguish rPDAC and mPDAC from healthy controls. These findings highlight the need for further investigation to validate SYPL-1 as a reliable, non-invasive biomarker that could enhance early detection, prognosis, and treatment monitoring in rPDAC
Temporomandibular Joint Sound Frequencies and Mouth-Opening Distances: Effect of Gender and Age
Get PDFBackground/Objectives: Temporomandibular joint disorders (TMDs) affect the temporomandibular joint and associated structures of the stomatognathic system. Joint Vibration Analysis (JVA) is a non-invasive method used to assess TMJ dysfunction through vibration frequencies. This study aimed to explore how age and gender influence TMJ vibration characteristics, hypothesizing that these factors may affect diagnostic accuracy in TMJ evaluations. Methods: This cross-sectional study includes 251 participants (143 females and 108 males) aged 10 to 30 years. TMJ evaluation used JVA to assess range of motion, integral values, and frequency distributions over and under 300 Hz. Participants with a history of TMJ disorders or significant maxillofacial trauma were excluded. Statistical analysis was conducted using employing Kolmogorov-Smirnov tests for data distribution, Kruskal-Wallis test for group comparisons, and Pearson correlation test for variable relationships. Results: Significant gender differences in range of motion (ROM) were observed, with males exhibiting higher values (p = 0.005). Age notably influenced vibration frequencies, particularly in total integral values (TIL and TIR) and frequency distributions around 300 Hz, suggesting links to degenerative changes. Females showed more pronounced age-related effects on vibration parameters. However, gender did not greatly affect vibration characteristics across all frequency bands, indicating that other factors also impact TMJ function. Conclusions: Age and gender significantly influence TMJ vibrations and the interpretation of JVA in clinical settings. Personalized approaches considering these demographic factors may enhance the accuracy of TMJ dysfunction diagnoses
A comprehensive examination of circadian rhythm and tryptophan pathway parameters: Assessing their role in predicting bipolar disorder in patients, siblings, and controls
No full textThe complex interplay between sleep, circadian rhythms, and tryptophan pathway metabolites in bipolar disorder (BD) remains poorly understood. This study examined these mechanisms in euthymic individuals with BD (n = 20), their unaffected siblings (n = 20), and healthy controls (n = 24). Subjective and objective sleep and circadian rhythm parameters were assessed using questionnaires and actigraphy, alongside plasma measurements of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), 3-hydroxykynurenine (3-HK), 3-hydroxyanthranilic acid (3-HAA), and quinolinic acid (QUIN). Compared to controls, both BD patients and siblings showed reduced sleep efficiency and increased wake after sleep onset. Patients had longer time in bed, whereas siblings had shorter total sleep time. Elevated KYN/TRP ratios were found in both groups. Higher 3-HAA levels predicted both BD and sibling status, while increased QUIN levels were specific to BD. Lasso regression identified 12 variables associated with BD and 6 with sibling status. These results suggest that altered tryptophan metabolism and circadian disruption may contribute to the pathophysiology of BD and familial risk, providing potential biomarkers for early identification and intervention