AXSIS (Yeditepe Üniversitesi)
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Zayıf over yanıtında büyümeyen foliküller için gonadotropinlere ara vermenin etkisi: Yeni bir strateji olabilir mi?
No full textObjective: Cessation of gonadotropin stimulation might affect follicular growth in patients in POSEIDON groups 3 and 4, which are unresponsive to high-dose stimulation. Materials and Methods: In this retrospective study, data were extracted from the medical records of patients treated at the Acıbadem Maslak Hospital Assisted Reproductive Technologies Unit between November 2010 and December 2020. Eighty-five patients who fulfilled the inclusion criteria were included in the study. Gonadotropin stimulation was discontinued if the follicle diameter increased by 2 mm within 7 days after the initiation of stimulation in patients in groups 3 and 4. The outcomes of the treatment strategy and pregnancy were recorded. Results: Follicular growth was observed in 40 (34/85) of patients, of whom 52.9 (18/34) had 2pn embryos. Ten of the 85 patients (11.8) underwent embryo transfer, resulting in biochemical pregnancy for two patients and healthy live birth for one patient. Conclusion: When high-dose stimulation is ineffective, discontinuing gonadotropin administration during ovarian stimulation may provide patients with the opportunity to conceive using their own biological oocytes. To the best of our knowledge, this is the first study to report a live birth rate using this strategy. © 2024 The Author.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Investigation of miRNA-199a-5p Expression and its Clinical Association With LDL Cholesterol Levels in Atherosclerosis
No full textBackground/Aim: Atherosclerosis is a chronic and progressive pathological condition marked by the accumulation of lipids, fibrous materials, and inflammatory cells, within the arterial walls. MicroRNAs (miRNAs) are single-stranded, evolutionarily conserved, non-coding small RNAs, that play a pivotal role in controlling various pathophysiological cellular functions and molecular signalling cascades associated with the development of atherosclerosis. Additionally, dysregulation in cholesterol and lipid metabolism is known to increase susceptibility to atherosclerosis. In this study, we aimed to determine the changes in serum levels of miRNA-199a-5p, examine its relationship with LDL cholesterol, and investigate its diagnostic value in patients diagnosed with atherosclerosis. Materials and Methods: MiRNA-199a-5p expression analysis was conducted using PCR on serum samples from 20 patients diagnosed with atherosclerosis and 26 completely healthy, voluntary control subjects. The blood biochemical analysis values for all groups participating in the study were obtained from their records. Results: The data analysis revealed significant up-regulation of miRNA-199a-5p in the serum of the patient group. Additionally, miRNA-199a-5p expression levels positively correlated with LDL cholesterol levels. Conclusion: miRNA-199a-5p can be considered a reliable biomarker in patients with atherosclerosis, potentially informing and guiding future therapeutic approaches. Additionally, a significant relationship was found between lipid metabolism and miRNA-199a-5p in atherosclerosis. © 2024 The Author(s).The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Medical management of chronic subdural hematoma with low-dose hydrocortisone: a case series
No full textChronic subdural hematomas (cSDH) are often managed with a burr-hole craniostomy and drainage, but surgery is associated with elevated mortality, morbidity, and recurrence. Despite reports of steroid use for such patients, its efficacy and feasibility are still debated. We present our patient series treated with low-dose hydrocortisone. We retrospectively reviewed data from patients treated with hydrocortisone between 2017 and 2023. Demographics, clinical and radiological data were collected. Of 27 patients identified, nine required a burr-hole craniotomy for an average volume of 120.23 cm3, average midline shift of 9mm, and neurological deficits. Eighteen met the criteria for inclusion. The mean age was 78.5 years; 13 were male. None had severe symptoms requiring urgent intervention. Except for one with a Karnofsky Performance Scale score of 70, all could maintain normal activity before treatment. The mean baseline volume was 52.6 cm3. Midline shift, present in six, averaged 6.8mm. Patients underwent treatment for an average of 5.15 months. Nine had complete resolution within 3 months, while nine required longer treatment, including one who needed 9 months for a re-bleed after a fall. Paired t-tests indicated significant reductions in hematoma volumes at the second week (p 0.01), first month (p < 0.0001), and third month (p < 0.0001) of treatment. No complications occurred and the post-treatment Karnofsky scores ranged from 90 to 100. Treatment for cSDH should be tailored to the patient. Low-dose hydrocortisone is safe and effective in asymptomatic patients, those with mild to moderate symptoms, and those who are either unsuitable for or decline surgical intervention. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Enhancing drought, heat shock, and combined stress tolerance inMyrobalan29C rootstocks with foliar application of potassium nitrate
No full textBackground: Drought and heat stress are significant concerns to food security in arid and semi-arid regions, where global warming is predicted to increase both frequency and severity. To cope with these challenges, the use of drought-tolerant plants or technological interventions are essential. In this study, the effects of foliar potassium nitrate (KNO3) application on the stress tolerance and recovery of Myrobalan 29C rootstocks (Prunus cerasifera Ehrh.) were evaluated. These rootstocks are widely recognized for their adaptability and are extensively used in fruit production. To assess their response, the rootstocks were subjected to drought, heat shock, or a combination of both stressors. Additionally, they were treated with 1.0 KNO3 via foliar application. Throughout the stress and recovery periods, various morphological, physiological, and bio-chemical parameters were measured. Results: Based on our results, KNO3 treatment improved LRWC, Chl stability, SC, and key stress markers like proline, MDA, H2O2, along with antioxidant enzymes CAT, SOD, POD during both stress and recovery phases. Moreover, our results emphasized KNO3's critical role in hormone regulation under stress. KNO3 application significantly altered hormone levels, notably increasing ABA during drought and heat shock stress, essential for stress response and adaptation. In contrast, IAA, GA, and cytokinin’s significantly increased during the recovery phase in KNO3-treated plants, indicating improved growth regulation and stress recovery. In addition, KNO3 application improved the recovery process of the rootstocks by restoring their physiological and biochemical functions. Conclusion: This study suggests that the application of foliar KNO3 is an effective technique for enhancing the drought and heat tolerance as well as the recovery of Myrobalan 29C rootstocks. These results hold significant value for farmers, policymakers, and researchers, as they offer crucial insights into the development of drought-tolerant crops and the management of climate change’s adverse effects on agriculture. © The Author(s) 2024.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Proprioseptif Nöromusküler Fasilitasyonun Servikal Eklem Hareket Açıklığı, Kuvvet Ve Propriyosepsiyon Üzerine Akut Etkisi
No full textPurpose: This double-blind randomized controlled study aimed to investigate the acute influence of two different proprioceptive neuromuscular facilitation (PNF) exercise, targeting stretching and strengthening, on cervical proprioception, range of motion (ROM) and strength among healthy university students. Methods: Healthy subjects were randomly divided into three groups as PNF stretching (PNFS) (n36), resistive PNF pattern (PNFP) (n35), and control group (CG) (n33) which received only passive range of motion (ROM) exercises without causing any stretch. All participants were assessed in terms of cervical proprioception, ROM and muscle strength before and after one intervention session. Results: Within-group analysis of the PNFS group showed a significant difference only in extension proprioception and right rotation ROM (p≤0.05) while the PNFP group showed a significant difference in extension, right rotation, right and left lateral flexion proprioception; extension and right rotation ROM, and right and left rotation muscle strength (p≤0.05). For the CG, the within-group analysis showed a significant difference in flexion, extension, right rotation and right lateral flexion proprioception, extension, and left and right lateral flexion ROM (p≤0.05). Between-group analysis showed a significant difference only in cervical flexion proprioception (p0.023) for PNFP over the CG. Conclusion: Although a lack of significant difference found in the between-group analysis, the within-group analysis showed that PNF patterns applied with resistance may be a promising technique to improve cervical proprioception, muscle strength and ROM. © 2024 Turkish Physiotherapy Association. All rights reserved.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Gold/platinum nanorods/temozolomide-UiO-66-NH2 metal-organic frameworks incorporated to chitosan-grafted polycaprolactone/polycaprolactone core-shell nanofibers for glioblastoma treatment during chemo-photothermal therapy
No full textThe use of biocompatible metal-organic frameworks (MOFs) and electrospun nanofibrous implants shows promise in preventing the recurrence of postsurgical glioblastoma. In this study, temozolomide (TMZ) and platinum‑gold nanorods (Pt[sbnd]Au NRs) were encapsulated into the UiO-66-NH2 MOFs. These were then incorporated into the chitosan-grafted polycaprolactone (PCL) (core)/PCL (shell) nanofibers coated with Pt[sbnd]Au NRs for extended release of TMZ during chemo-photothermal therapy against glioblastoma cells. The drug encapsulation efficiency, TMZ release, and in vitro cell viability were investigated for the MOFs, simple nanofibers, core-shell nanofibers, and MOFs-nanofibers. The extended release of TMZ occurred over 44 and 36 days from the core-shell nanofibers coated with Pt[sbnd]Au NRs under NIR irradiation at pH values of 7.4 and 5, respectively. The maximum killing of U87 glioblastoma cells was 80.2 using TMZ-Pt-Au-MOF-core-shell nanofibers coated with Pt[sbnd]Au under NIR irradiation. The relative tumor size for the mice bearing glioblastoma and treated with pure core-shell nanofibers, TMZ-Pt-Au-MOF, and TMZ-Pt-Au-MOF-core-shell nanofibers coated with Pt[sbnd]Au without NIR irradiation and with NIR irradiation was 4.12, 2.12, 1.65, 0.86, and 0.48, respectively, after 30 days. The synthesized MOF-core-shell nanofibers-Pt-Au NRs implantable device shows potential as a new approach for postsurgical glioblastoma treatment during chemo-photothermal therapy. © 2024 Elsevier B.V.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Development of new gallic acid loaded films for wound dressings: In vitro and in vivo evaluations
No full textWounds that cannot be treated and become chronic remain among the most challenging health problems with financial burdens worldwide. Herein, gallic acid (GA) loaded polymeric films were developed using HPMC (Hydroxypropyl methylcellulose) or sodium alginate as the main polymer, and characterizations were examined. Weights were found between 681.25 ± 29.77 and 329.25 ± 4.21 mg and the thickness was in between 1.40 ± 0.09 and 0.70 ± 0.01 mm range. pH values were quite close to neutral. Moisture uptake and loss results were in harmony with plasticizer concentration that was also observed with texture profile analysis such as elongation at break 74.45 ± 2.70 which corresponds to tensile strength 0.034 ± 0.005 N/mm2 for H4 that is suitable for wound application. Swelling of the films was found higher with sodium alginate-containing formulations compared to HPMC-based films due to lower molecular weight. In vitro release study exhibited that the release of GA from both HPMC and sodium alginate-based films reached 100 in 3 h. Ex vivo permeation and penetration were found to be low which is preferred for wound treatment. In vitro biological assays were assessed in terms of in vitro antioxidant capacity, in vitro wound healing assays, and in vitro anti-inflammatory evaluations. H4 indicated the highest free radical scavenging activity and the highest dose-dependent wound healing potential with 1 mg/ml (96 on the 24th hour). Finally, in vivo studies were performed on mice with control, market product, blank, and GA-loaded films and histopathological assessment. All findings revealed that the film containing GA, HPMC, PVP (polyvinylpyrrolidone), and propylene glycol is an outstanding alternative wound therapy. © 2024 Elsevier B.V.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Introducing the ELPIS scale: a novel tool for measuring emotional empathy toward cancer patients
No full textEmpathy, defined as the ability to understand and share another person’s feelings, is crucial in the healthcare setting, particularly for patients with cancer who face significant psychosocial challenges. We aimed to develop a novel tool, the Empathy, Life with Cancer, and Psychosocial Skills (ELPIS) Scale that prioritizes emotional and cognitive components in order to determine the reflection of the negative stigmatization of cancer in societies on individuals, excluding the behavioral and ethical dimensions of empathy. The study was conducted using a quantitative research design with a sample of first- and second-year medical students in Türkiye. An initial pool of 48 items was developed based on an extensive review of the literature and existing empathy scales. Following expert review and pilot testing, the scale was refined to 24 items. Exploratory factor analysis and confirmatory factor analysis were conducted to establish the scale’s factor structure and validate its reliability and validity. The exploratory factor analysis revealed a three-factor structure comprising “emotional empathy,” “sympathetic responses,” and “realistic approaches,” with a total of 20 items after refinement. Confirmatory factor analysis confirmed the model fit, with the scale demonstrating high internal consistency across all three dimensions. The scale’s reliability was further supported by high Cronbach’s alpha values. The results suggest that the ELPIS Scale is a reliable and valid instrument for measuring emotional empathy toward cancer patients. It offers a nuanced approach by separately assessing emotional, sympathetic, and realistic components of empathy, making it a valuable tool for both educational and clinical applications. Future research should explore the scale’s application in diverse populations and settings, as well as its utility in tracking empathy development over time. In conclusion, the ELPIS Scale, which focuses on the emotional and cognitive components of empathy, a multifaceted construct, fills a critical gap in the measurement of empathy in the context of cancer care and provides a comprehensive tool that can improve both patient care and the education of healthcare providers. © The Author(s), under exclusive licence to Springer ScienceBusiness Media, LLC, part of Springer Nature 2024.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Extracellular vesicles derived from human foreskin cells (hFS-Exo) accelerate cell migration and angiogenesis through MAPK pathway: an in vitro study
No full textBackground: Wound healing is one of the important processes in the body. Attempts to create new drugs are of interest due to the side effects of natural and chemical wound healing compounds. To overcome this obstacle, stem cells have been used as healing agents. However, both difficulties in collection and risks such as rejection and teratoma in the recipient body have limited the use of stem cells, directly. Since the potential content of the stem cells can be transferred to the recipient cells by vesicles, small extracellular vesicles have recently become prominent agents. Methods and results: The wound-healing effect of extracellular vesicles derived from foreskin cells was investigated in both keratinocyte and endothelial cells. Migration assay, RT-PCR, Col1a1 ELISA and Western Blot experiments were utilized to reveal healing effect of EVs and its possible molecular pathways. EV-treated groups exhibited more proliferative, invasive, and migrative characteristics. When comparing to the control group, new vessel formation was induced in EV groups. An increase in gene levels of growth factors related to wound healing and change in the mitogen-activated protein kinase (MAPK) signaling pathway proteins in EV-treated groups were determined. Possible molecular mechanisms underlying cell movements were associated with the MAPK pathway. It was found that human foreskin cell EVs (hFS-Exo) may have a potential to heal wounds in a short period of time by triggering the MAPK pathway. Conclusions: hFS-Exo could be a new promising wound healing agent in the future. © The Author(s), under exclusive licence to Springer Nature B.V. 2024.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive
Conditioned medium of induced pluripotent stem cell derived neuromesodermal progenitors enhances cell migration in vitro
No full textBackground: Identification of novel cell-based therapy sources has been of great interest in recent years to provide alternative and available therapy options in clinics. Conditioned medium (CM) can be a valuable supply for growth factors, cytokines and chemokines as a source of stem cell secretome. Exploring the role of new CM sources for tissue regeneration might be a promising approach for therapeutic purposes. Methods and results: In the current study, neuromesodermal progenitors (NMPs) derived from induced pluripotent stem cells (iPSCs) were used to collect CM. Fibroblast derived iPSCs were successfully differentiated into NMPs and NMPs were characterized by double positive T/Bra and Sox2 staining. CM was collected from NMPs, and the content was characterized by membrane analysis. In vitro wound healing assay was used as a model system to observe potential activity of CM on cell migration. Fibroblasts, keratinocytes and endothelial cells were used to evaluate the effect of NMP-derived CM (NMP-CM) on cell migration in vitro. Several important proteins related to wound healing such as ANGPT 1, ANGPT 2, MCP-1, PDGF-AA, SDF-1α, TIMP-1 and TIMP-2 were increased in NMP-CM. NMP-CM increased cell proliferation and migration in vitro. Conclusions: In vitro data obtained from three distinct cell types suggest a promising role of NMP-CM on cell migration. NMP-CM can be used for wound management in the further future after detailed in vitro and in vivo research. © The Author(s), under exclusive licence to Springer Nature B.V. 2024.The Yeditepe University Open Access System is a compendium of indexed publications. Copyright and usage rights may be held by publishers and/or authors. The collection was established for the scientific publications prepared by Yeditepe University academicians and is to be found in the institutional archive.Methods and results: In the current study, neuromesodermal progenitors (NMPs) derived from induced pluripotent stem cells (iPSCs) were used to collect CM. Fibroblast derived iPSCs were successfully differentiated into NMPs and NMPs were characterized by double positive T/Bra and Sox2 staining. CM was collected from NMPs, and the content was characterized by membrane analysis. In vitro wound healing assay was used as a model system to observe potential activity of CM on cell migration. Fibroblasts, keratinocytes and endothelial cells were used to evaluate the effect of NMP-derived CM (NMP-CM) on cell migration in vitro. Several important proteins related to wound healing such as ANGPT 1, ANGPT 2, MCP-1, PDGF-AA, SDF-1α, TIMP-1 and TIMP-2 were increased in NMP-CM. NMP-CM increased cell proliferation and migration in vitro