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Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis
No full textCymbopogon martinii is an aromatic crop cultivated for the production of its essential oil (EO) which has different applications. Bearing in mind that C. martinii EO (CMEO) has already been proved for high antibacterial potential the aim of this study was to formulate stable CMEO nanoemulsions, being capable to combat Enterococcus faecalis biofilm, especially within infected tooth’s root canal. Ten emulsions with variable CMEO content (2.5–10 % v/v) and Tween 80 (T80, 2.5–15 % v/v) were formulated (designated as ECMEO:T80) and screened for volume-weighted mean diameter (D4,3) and stability, using dynamic light scattering measurements. Chemical composition of stable nanoemulsions was monitored by UV–VIS and FTIR, while their antibacterial activity was estimated in microdilution assay. Antibiofilm properties of selected nanoemulsions were tested in vitro (cristal violet assay) and ex vivo (within root canals of the extracted teeth). Results showed that formulations containing 10 % of T80 and CMEO volume ≤ 6 %, as well as those having constant 2.5 % of CMEO and variable T80 volume (≤ 10%), namely E2.5:2.5, E2.5:5, E2.5:7.5, E2.5:10, E4:10, and E6:10, were stable and nanosized (D4,3 <100 nm). FTIR and UV–VIS analyses confirmed successful encapsulation of the CMEO by T80 and showed that nanoemulsification did not affect CMEO composition. Evaluation of antibacterial potential showed that the most efficient were E6:10, E2.5:2.5 and E4:10 (MIC values 0.37–1.97 mg mL−1). Analysis of dependency of determined MICs on mean diameter and CMEO volume showed that antibacterial potential increased with both parameters. E6:10, E2.5:2.5 and E4:10 also reduced in vitro biofilm (inhibitory range 17.6–58.4 %), as well as intracanal biofilm (reduction ~2Log CFU). Presented results suggest E6:10, E4:10 and E2.5:2.5 as the good candidates for further research
Isparljive materije u plodovima sorti i selekcija maline gajenih u zapadnoj Srbiji
No full textThis study presents the results of a research into volatile compounds in raspberry fruits of four commercial cultivars (‛Willamette’, ‛Meeker’, ‛Tulameen’, ‛Latham’) and one a selection (‛K 81/6’) grown in Western Serbia. By using the method of simultaneous distillation and extraction (Likens-Nickerson method), 37 volatile compounds were isolated and identified (using gas chromatography-mass spectrometry – GC/MS), and then classified into corresponding classes of aldehydes, ketones, acids, esters, terpenes, C13-norisoprenoids, sesquiterpenes and hydrocarbons. A quantitative analysis (using gas chromatography-flаmе iоnisаtiоn dеtеctоr – GC/FID) revealed that tested raspberry cultivars and selection differ in the content of individual volatile components (expressed in %). The highest share in volatile components belonged to the class of C13-norisoprenoids, among which the most abundant were β-ionone (ranging from 4.50% in ‛Latham’ to 26.79% in ‛K 81/6’ selection) and α-ionone (ranging from 5.04% in ‛Latham’ cv., to 11.43% in ‛Meeker’).Prоizvоdnjа mаlinе u Srbiјi u prеdhоdnih pеt gоdinа krеtаlа sе izmеđu 42.300 i 100.000 tоnа (prоsеk 70.400 tоnа), što svrstаvа našu zemlju u rеd vоdеćih svetskih prоizvоđаčа mаlinе. Zаpаdnа Srbiја је glаvnо prоizvоdnо pоdručје zа gајеnjе mаlinе. Nа izbоr sоrtе mаlinе kоја ćе sе gајiti nа оdrеđеnоm pоdručјu, pоrеd njеnih оdgајivаčkih kаrаktеristikа i prilаgоđеnоsti datim аgrо-еkоlоškim uslоvimа, prеsudnо utiču kvаlitеt i nаčin kоrišćеnjа plоdа. Kvalitet plodova se najčešće povezuje sa sеnzоrnim kаrаktеristikаmа plоdа (izglеd, kоnzistеnciја, ukus i аrоmа) koje zavise od brојnih kоmpоnеnata hеmiјskоg sаstаvа. Od ispаrlјivih kоmpоnеnаtа plоdа mаlinе zаvisi njеnа аrоmа, odnosno оvа јеdinjеnjа, zајеdnо sа šеćеrimа i kisеlinаmа, dајu spеcifičаn miris i ukus mаlinе. Оvе sеnzоrnе kаrаktеristikе su pаrаmеtri kvаlitеtа оd kојih, izmеđu оstаlоg, zаvisi prihvаtlјivоst plоdоvа rаzličitih sоrаtа mаlinе zа pоtrоšnju u svеžеm stаnju, kао i pоgоdnоst zа prеrаdu. Pošto se gotovo celokupan godišnji rod malina u Srbiji prerađuje, zа dоbiјаnjе visоkоg kvаlitеtа pојеdinih prоizvоdа, vаžnо је kоristiti sоrtе mаlinе kоје sе kаrаktеrišu, izmеđu оstаlоg, i izrаžеnоm karakterističnom аrоmоm.
U rаdu su prikаzаni rеzultаti ispitivаnjа isparljivih mаtеriја plоdоvа čеtiri sоrtе (Vilаmеt, Мikеr, Тјulаmin, Lејtаm) i јеdnе sеlеkciје (K 81/6) mаlinе gajenih u Zapadnoj Srbiji. Меtоdоm simultane destilacije i ekstrakcije (metod pо Lickens-Nickerson-u) izоlоvаnо је i identifikovano 37 аrоmаtičnih kоmpоnеnаtа, kоје su nаkоn idеntifikаciје (mеtоd GC/MS) svrstаnе u klase aldehida, ketona, kiselina, estara, terpena, C13 norizoprenoida, sеskvitеrpеnа i uglјovodonika. Među identifikovanim isparljivim jedinjenjima, 16 je nađeno u plodovima svih pet genotipova. Оstаlе kоmpоnеntе bilе su prisutnе u plоdоvimа sаmо pојеdinih gеnоtipоvа. Меđu ispаrlјivim јеdinjеnjimа kаrаktеrističnim zа svе gеnоtipоvе, 11 kоmpоnеnаtа spаdајu u grupu visоkо аrоmаtski pоtеntnih јеdinjеnjа i tо su 4 tеrpеnа (α-pinеn, limоnеn, linаlоl, α-tеrpinеоl), 4 C13-nоrizоprеnоidа (α-јоnоl, dihidrо α-јоnоn, α-јоnоn, β-јоnоn) i 3 аldеhidа (nоnаnаl, dеkаnаl, ciklаmа аldеhid).
Kvаntitаtivnоm аnаlizоm (GC/FID) је utvrđеnо dа sе plоdоvi ispitivаnih sоrаtа i sеlеkciје mаlinе rаzlikuјu pо sаdržајu pојеdinih kоmpоnеnаtа аrоmе (izrаžеnih u %). Nајzаstuplјеniје kоmpоnеntе аrоmе plоdоvа pripаdаlе su klаsi C13 nоrizоprеnоidа, оd kојih su nајzаstuplјеniјi bili β-јоnоn (оd 4,50% kоd sоrtе Lејtаm dо 26,79% kоd sеlеkciје K 81/6) i α-јоnоn (оd 5,04% kоd sоrtе Lејtаm dо 11,43% kоd sоrtе Мikеr).
Nа оsnоvu hiјеrаrhiјskе klаstеr аnаlizе ispitivаni gеnоtipоvi mаlinе sе mоgu svrstаti u dvа klаstеrа. U јеdnоm klаstеru sе nаlаzе gеnоtipоvi čiјi plоdоvi imајu izrаžеnu kаrаktеrističnu аrоmu mаlinе (Vilаmеt, Мikеr, Тјulаmin i K81/6), uz pоstојаnjе finih gеnоtipskih rаzlikа. U drugоm klаstеru sе nаlаzi sоrtа Lејtаm, čiјi је prоfil аrоmе pоtpunо drugаčiјi оd оstаlih gеnоtipоvа
Innovative and sustainable practices for fruit growing
No full textPoljoprivrednu proizvodnju poslednjih decenija prati degradacija životne sredine koja ugrožava same sisteme od kojih zavisi naš budući razvoj. Pored toga, konvencionalni sistemi poljoprivredne proizvodnje dostigli su svoj maksimum u smislu povećanja produktivnosti i korišćenja zemljišta. Shodno tome, istraživanja u oblasti biotehnologije postaju sve aktuelnija, a pronalaženje odgovarajućih eko-bezbednih preparata i uvođenje inovativnih i po životnu sredinu bezbednih tehnika u voćarsku proizvodnu praksu predstavljaju veliki izazov za naučnike širom sveta. Optimizacija procesa dobijanja mikrobne biomase, kao i tehnološkog postupka formulacije mikrobioloških preparata sa potencijalnim biopesticidnim i fitostimulatornim efektom predstavljaju odličnu alternativu i veliku šansu koja može odgovoriti gore pomenutim izazovima.
U radu su opisana najnovija iskustva i rezultati bazirani na korišćenju prirodnih resursa i razvoju novih biotehnoloških rešenja koja mogu podstaći rast i prinos voćaka i poboljšati nutritivnu vrednost plodova. U tom smislu, posebna pažnja je posvećena rezultatima dosadašnjih proučavanja efikasnosti primene jednog inovativnog biopreparata na bazi vermikomposta, obogaćenog odabranim sojevima korisnih mikroorganizama (BioVermix), razvijenog u Institutu za voćarstvo Čačak. Akcenat je stavljen na mehanizame njegovog dejstva i praktične primere njegove primene, kao nove, bioracionalne metode u voćarskoj proizvodnoj praksi.
Rezultati sprovedenih istraživanja pokazali su da primena BioVermix-a u zasadima šljive, jagode, aronije, kupine i borovnice omogućava postizanje stabilnih prinosa i visok kvalitet ploda sa povećanim sadržajem fenolnih jedinjenja odgovornih za antioksidativni kapacitet. Konačno, sve ovo zajedno omogućava kreiranje sistema input-primarni-finalni proizvod bez negativnih posledica na ljudsko zdravlje i životnu sredinu.Agricultural production in recent decades has been accompanied by environmental degradation that affects the very systems on which our future development depends. Besides, conventional agricultural production has reached its maximum in terms of increasing productivity and land use. Accordingly, research in the field of biotechnology is becoming more and more relevant, and discovering appropriate eco-safe preparations and introducing innovative and environmentally safe techniques into fruit growing is a major challenge for scientists around the world.Optimization of the production of microbial biomass and formulation of microbiаl preparations with potential biopesticidal and phytostimulatory action are an excellent alternative which may respond to the challenges mentioned above. In this regard, primarily due to the emergence of environmental awareness of consumers and as well as human health.
The study describes the latest experiences and results based on the use of natural resources and development of new biotechnological solutions that can stimulate fruit growth and yielding and improve the nutritional value of fruits as well. In that sense, special attention is paid to the results of previous studies on the efficiency of one innovative biopreparation based on vermicompost, enriched with selected strains of beneficial microorganisms (BioVermix), developed at the Fruit Research Institute, Čačak. Emphasis is placed on the mechanisms of its action and practical examples of its application, as new, biorational methods in fruit growing.
The results of the research showed that the application of BioVermix in plum orchards and plantings of strawberries, chokeberries, blackberries and blueberries enables stable yields and high quality fruits with increased content of phenolic compounds responsible for antioxidant capacity. Finally all this together enables fruit growers to create of an input-primary-final product system without negative effects on human health and the environment
Quality of fruit spirits from new stone fruit genotypes obtained using two common processing procedures in Serbia
No full textNovostvorene genotipove voća treba ispitati u pogledu pogodnosti za različite načine korišćenja, između ostalog i za proizvodnju voćnih rakija. Kako bi se dobile voćne rakije koje odgovaraju zahtevima zakonske regulative i zadovoljavaju ukus potrošača, neophodno je prilagoditi način prerade svakom genotipu. U radu su prikazani rezultati ispitivanja kvaliteta voćnih rakija, dobijenih na dva različita načina, od pet novih genotipova koštičavog voća, koji su stvoreni u Institutu za voćarstvo, Čačak – 3 genotipa šljive (mildora, nada, hibrid 22/17/87) i 2 genotipa višnje (šumadinka i sofija). U Srbiji se rakije od koštičavog voća proizvode najčešće na dva načina, koji podrazumevaju različitu pripremu plodova za alkoholnu fermentaciju – muljanje plodova bez odvajanja koštica (tradicionalni postupak koji koriste prvenstveno mali proizvođači) i pasiranje plodova uz istovremeno odvajanje koštica (postupak koji je uobičajen u bolje opremljenim destilerijama). Voćni kljukovi svih pet ispitivanih genotipova, koji su pripremljeni na ova dva načina, su spontano fermentisali i destilisani su odmah po završenoj alkoholnoj fermentaciji. Korišćena je dvostruka destilacija u alambiku tradicionalne konstrukcije. Sve proizvedene rakije zadovoljavale su zahteve zakonske regulative, a po senzornom kvalitetu su klasifikovane u grupu kvalitetnih rakija. Različiti načini prerade plodova istog genotipa uslovili su pojavu razlika u sadržaju pojedinih komponenata rakija, kao i pojavu finih razlika u senzornim karakteristikama. U poređenju sa rakijama dobijenim tradicionalnim postupkom, voćne rakije od ispasiranog kljuka bez koštica sadržale su više metanola, acetaldehida, 1-propanola i 1-heksanola, a manje koncentracije etilacetata i benzaldehida. Prerada plodova sa košticama (tradicionalni postupak) dala je nešto bolji senzorni kvalitet rakija od sorte šljive nada i sorata višnje šumadinka i sofija, dok je kod ostalih ispitivanih genotipova šljive utvrđeno suprotno.Newly created fruit genotypes should be examined for suitability for different uses, including fruit spirit production. In order to obtain fruit spirits that meet the requirements of legislation and satisfy consumers' taste, the method of processing should be adapted to each genotype. This paper presents the results of analysis of quality parameters of stone fruit spirits, from five new genotypes created at the Fruit Research Institute, Čačak – three plum genotypes (ˊMildoraˊ, ˊNadaˊ, ˊHybrid 22/17/87ˊ) and two sour cherry genotypes (ˊŠumadinkaˊ and ˊSofijaˊ), obtained in two different ways. In Serbia, stone fruit spirits are usually produced in two ways, which include different ways of preparing fruits for alcoholic fermentation - fruit crushing without removing the stones (traditional process used by small producers) and fruit pulping with simultaneous separation of stones (process used in better equipped distilleries). Fruit mashes of all five examined genotypes, prepared in these two ways, were spontaneously fermented and distilled immediately after the completion of alcoholic fermentation. Double distillation in alambic of traditional construction was used. All produced fruit spirits met the requirements of legislation and, according to sensory quality, were classified in the group of quality fruit spirits. Different ways of processing fruits of the same genotype caused the differences in the content of individual components of spirits, as well as the fine differences in sensory characteristics. Compared to fruit spirits obtained by the traditional method, fruit spirits made from pulped fruit mashes without stones contained more methanol, acetaldehyde, 1- propanol and 1-hexanol, and less ethyl acetate and benzaldehyde. If the stones were not removed during processing (traditional process), a slightly better sensory qualities were obtained in fruit spirits of ˊNadaˊ plum cultivar and ˊŠumadinkaˊ and ˊSofijaˊ sour cherry cultivars, while the opposite was found in fruit spirits from other examined plum genotypes (ˊMildoraˊ and ˊHybryd 22/17/87ˊ)
An overview of plum breeding at Fruit Research Institute, Čačak
No full textPlum breeding work at the Fruit Research Institute, Čačak, which has been implemented since founding of the Institute in 1946, has led to the development of very important European plum (Prunus domestica L.) cultivars. Namely, cultivars such as ‘Čačanska Lepotica’, ‘Čačanska Rodna’, ‘Čačanska Najbolja’ and ‘Čačanska Rana’ are well known worldwide and have been grown in commercial orchards, as well as used as parents in different plum breeding programmes. Planned hybridization was used as the most important method in breeding. During the programme realization, the main breeding objectives were fruit quality, high productivity, early and late ripening time, tolerance to ecological conditions as well as diseases, especially Sharka virus. In the previous period, 14 cultivars were developed. Thereafter, four cultivars were released in the last eight years: ‘Nada’ in 2012, ‘Divna’ and ‘Petra’ in 2018 and ‘Lana’ in 2020. Along with planned hybridization, clonal selection of the most important autochthonous cultivars has been carried out. Clones with larger fruit, better fruit quality and tolerant to stress factors and diseases are singled out and will be used in further breeding work or will be introduced into production, especially as a raw material for the brandy production. Therewith, research is also focused on plum floral biology which includes pollen quality and pollen tubes growth in the pistil observations, as well as cytoembryology with the aim of determining the degree of self-(in)compatibility and the most suitable pollenisers for self-incompatible cultivars
Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration
No full textThis paper investigates the influence of composition of vitrification solution on the regrowth capacity of apple 'Gala Must' (Malus x domestica Borkh.) cryopreserved by a vitrification technique. In vitro-grown shoot tips were precultured in the dark at 23 degrees C, in a liquid MS medium with two sucrose treatments (0.3 M for 15 h, then 0.7 M for 5 h). After loading at room temperature with solution containing 2 M glycerol and 0.4 M sucrose for 20 min, explants were dehydrated at 0 degrees C for 30, 40 and 50 min. Dehydration was done using either original PVS2 (13.7% w/v sucrose, 30% w/v glycerol, 15% w/v ethylene glycol, 15% w/v DMSO) or modified PVS2 solution (PVS A3 - 22.5% w/v sucrose, 37.5% w/v glycerol, 15% w/v ethylene glycol and 15% w/v DMSO). The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 40 and 85 and 20-40%, respectively. The highest values of both parameters were achieved with longest treatment duration. Dehydration with the PVS A3 solution resulted in considerably higher survival rates (68.2-90%), as well as higher regrowth rates (15-75%) after cryopreservation. Fifty-min treatment with this vitrification solution led to a significant increase in the percentage of regrowth (up to 75%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3 based vitrification technique for a long-term storage of this genotype
Nut fruit growing in Serbia – State and Perspectives
No full textFruit growing is a very important branch of agriculture in the Republic of Serbia. Fruit production accounts for about 11% of agriculture production value (Development Strategy of Agriculture of the Republic of Serbia 2014-2024). Natural conditions of our country, especially some of the regions, are very favorable for the cultivation of fruit, including nut fruit. In Serbia of nut fruit the most grown are walnuts, whereas a growing interest in hazelnut production has been observed over the last years. However, the share of plantations covered by these species is not larger than 3.93% of fruit plantation structure. According to Census for Agriculture in the Republic of Serbia (2012), Serbia has over 1,3 million walnut trees and over 1,1 million hazelnut trees. However, in the period 2013-2019, a decrease in the number of productive walnut trees, and an increase of the number of hazel trees was recorded. Namely, the analysis of the available data showed that the total production of walnuts in this period varied from 8,836 to 19,058 tonnes, while production of hazelnut ranged from 3,366 to 5,428 tonnes, depending on the year (FAOSTAT, 2021). Generally, in the period from 2013 to 2019 in the Republic of Serbia there was a decrease in total production of walnuts for 10,222 t, while for the same period there was an increase in production of hazelnuts for 1,583 t. Therefore, the objective of this study was to perform analysis state and perspectives of walnut and hazelnut production in different regions of the Republic of Serbia
Istorijski razvoj biljne virusologije u Srbiji
No full textNeprestani demografski rast sveta i potreba da se stanovništvo snabde prirodnim
proizvodima i hranom kontrolisanog kvaliteta, nametnuli su potrebu proučavanja virusnih
bolesti biljaka. Osim praktičnog značaja u razvijanju uspešnih strategija u suzbijanju virusa i
sprečavanju ili umanjenju šteta izazvanih virusima, biljna virusologija ima i veliki
fundamentalni značaj u otkrivanju bioloških, epidemioloških i evolutivnih zakonitosti i pojava.
Otkriće biljnih virusa vezuje se za 1898. godinu kada je Martinus Willem Beijerinck
proučavajući mozak duvana ukazao na novi svet infektivnih agenasa („contagium vivum
fluidum“). U Srbiji tridesetih godina prošlog veka, proučavanja biljnih virusa započela su
naučnim radom Mladena Josifovića na šarki šljive. Posle Drugog svetskog rata, Dragoljub
Šutić, Veljko Nikolić i Mitar Jordović uvode naučne eksperimentalne metode u istraživanja
viroza, i time postavljaju temelje razvoja proučavanja biljnih virusa kod nas. Razvoj
virusologije u Srbiji, kao i u većini zemalja, obuhvatao je nekoliko faza: početnu deskriptivnu
(otkrivanje i opisivanje brojnih viroza), potom epidemiološku (proučavanje načina održavanja
i širenja virusa), fizičko-hemijsku (proučavanje morfoloških osobina i hemijskog sastava
virusa), molekularno-biološku fazu (razumevanje strukture i funkcije genoma virusa,
mehanizama replikacije, unapređenje starih i uvođenje novih metoda detekcije i identifikacije
virusa i njihovih sojeva, pravilna taksonomija virusa u skladu sa poreklom i evolutivnim
međuodnosima). Baveći se različitim oblastima biljne virusologije u proteklih gotovo osam
decenija, brojni virusolozi ostvarili su značajna dostignuća. Posebne zasluge za razvoj biljne
virusologije svakako pripadaju Dragoljubu Šutiću, koji je zahvaljujući svom širokim
obrazovanju, uspešnom eksperimetalnom radu, dobro razvijenoj domaćoj i međunarodnoj
saradnji, ostvarenim rezultatima i brojnim naučnim radovima i publikacijama, značajno
doprineo obrazovanju mnogih generacija studenata, i dao nemerljiv doprinos unapređenju zdravlja bilja. Razvoj biljne virusologije u našoj zemlji nije zavisio samo od opšteg napretka
ove nauke u svetu, već u velikoj meri i od uslova za razvoj naučnih istraživanja. I pored
relativno skromnih uslova koji vladaju na naučno-istraživačkoj sceni virusologije, rad biljnih
virusologa u proteklom periodu podigao je kvalitet istraživanja trudeći se da dostigne i ne
zaostaje za kvalitetom razvijenih zemalja. Značajno iskustvo u decenijskom radu u oblasti
razvojnih i primenjenih istraživanja u biljnoj virusologiji i sva stečena saznanja o biljnim
virusima bila su, i sada su od neprocenjivog značaja za poljoprivredne proizvođače i
unapređenje njihove proizvodnje, za unapređenje sistema i efikasnosti karantinske i
fitosanitarne službe, rad ovlašćenih dijagnostičkih laboratorija, ispunjavanje međunarodnih
fitosanitarnih zahteva u prometu i razmeni biljnog materijala, i podizanju ugleda Srbije.
Većina publikacija je rezultat istraživanja etiologije, simptomatologije, epidemiologije,
genetičke strukture populacije, otpornosti prema virusima i drugih mera kontrole sa ciljem
iznalaženja rešenja od praktičnog značaja.
Iako je razvoj biljne virusologije u Srbiji pokrio mnoge fundamentalne i praktične
aspekte ove nauke, očekuju se nastavak istraživanja zasnovanih na mogućnostima koja nam
pružaju druge nauke, pre svega molekularna biologija i bioinformatika. Zalog za sigurnu
budućnost virusologije u našoj zemlji je obrazovanje i osposobljavanje mladih istraživača
Variation in productivity and fruit quality of the plum depending on rootstock
No full textDespite Serbia being among the world's largest producers of plums with about 5% of world production, seedlings of ‘Myrobalan’ (Prunus cerasifera Ehrh.), characterized by a number of disadvantages, represent the most commonly used plum rootstock. Considering the intensification of plum production in the orchards of the Serbia and other European countries, this study was aimed to assess the productivity and fruit quality of two plum cultivars (‘Čačanska Lepotica’ and ‘Čacanska Najbolja’) grafted on four different rootstocks (‘Myrobalan’, ‘Fereley’, ‘St. Julien A’ and ‘Pixy’) for two consecutive years (2017–2018). Cultivars ‘Čačanska Lepotica’ and ‘Čacanska Najbolja’ produced similar average yield per tree (10.9 and 10.8 kg tree-1, respectively), while fruit weight, content of total phenols and antioxidant activity were significantly higher in ‘Čačanska Najbolja’ samples compared to ‘Čačanska Lepotica’, regardless rootstock. Yield per tree in plum grafted on ‘Myrobalan’ was higher compared to other rootstocks in both years, except in cultivar ‘Čačanska Najbolja’ in 2017, when the yield per tree was low in all studied rootstocks. Dwarf rootstock ʻPixyʼ showed the lowest yield per tree and induced high total phenols and antioxidant activity in fruit of cultivar ‘Čačanska Lepotica’ in both year of testing. However, in cultivar ‘Čačanska Najbolja’ there was no general trend in yield and fruit quality depending on rootstock. The medium vigor rootstocks ‘Fereley’ and ‘St. Julien A’ induced high values of yield per tree and content of total phenols in cultivar ‘Čačanska Lepotica’, although no significant differences were found with rootstock ‘Myrobalan’. Owing to the high amounts of beneficial phytochemicals cultivar ‘Čačanska Najbolja’ can be recommended for expansion in plum growing regions. Using semi-dwarf rootstocks ‘Fereley’ and ‘St. Julien A’, planting density can be increased, as well as yield and fruit quality, and facilitate application agricultural practices, such as pruning and harvesting
