Open Library Archives of Kagawa University (OLIVE)
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PLAAT1 expression triggers fragmentation of mitochondria in an enzyme activity-dependent manner
Get PDF香川大学Kagawa University博士（医学）The phospholipase A and acyltransferase (PLAAT) family is a protein family consisting of five members (PLAAT1–5), which acts as phospholipid-metabolizing enzymes with phospholipase A1/A2 and N-acyltransferase activities. Since we previously reported that the overexpression of PLAAT3 in mammalian cells causes the specific disappearance of peroxisomes, in the present study we examined a possible effect of PLAAT1 on organelles. We prepared HEK293 cells expressing mouse PLAAT1 in a doxycycline-dependent manner and found that the overexpression of PLAAT1 resulted in the transformation of mitochondria from the original long rod shape to a round shape, as well as their fragmentation. In contrast, the overexpression of a catalytically inactive point mutant of PLAAT1 did not generate any morphological change in mitochondria, suggesting the involvement of catalytic activity. PLAAT1 expression also caused the reduction of peroxisomes, while the levels of the marker proteins for ER, Golgi apparatus and lysosomes were almost unchanged. In PLAAT1-expressing cells, the level of dynamin-related protein 1 responsible for mitochondrial fission was increased, whereas those of optic atrophy 1 and mitofusin 2, both of which are responsible for mitochondrial fusion, were reduced. These results suggest a novel role of PLAAT1 in the regulation of mitochondrial biogenesis.doctoral thesi
ERRATUM : Designing the theory for studying on teaching materials under the updating Japanese government policy in education [Bull. educ. res. teach. develop. Kagawa Univ. 45 : 33-39, 2022]
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The Origin of Regional Resources : A Case Study of Olives on Shodoshima Island
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