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DATA COMPILATION: Focus Groups Transcripts (PRO-SUEDAD Research Project)
No full textThis data examines perceptions of ageing, ageism, and profiguration through three intergenerational focus groups. Participants question the dominant model of “active ageing,” arguing that it often imposes normative expectations of productivity and overlooks diverse abilities, desires, and life circumstances. Ageism emerges as a bidirectional phenomenon affecting both older adults and younger people, whose emotions and experiences are frequently undervalued. Profiguration is viewed positively as an interdependent and socially sustainable framework that counters growing individualism and unwanted loneliness. The discussions also highlight the pressures associated with demographic change, the externalisation of care, and the need for public policies that strengthen community ties and promote meaningful intergenerational encounters
Research Synthesis of Task-Based Needs Analysis Methodologies in Language Education, 1999–2025: Comparative Study of 33 TBNAs Across Languages, Contexts, and Learner Populations
No full textThis dataset contains a structured synthesis of task-based needs analyses in the field if applied linguistics from 1999 to 2025. It includes detailed information on 33 studies, covering authorship, target learner groups, target languages, research methodologies, instruments used, stakeholders involved, contributions from domain experts, and complete bibliographic references.
The dataset is designed to support systematic comparison and analysis of research methodologies in task-based needs analysis across different educational contexts, languages, learner populations, and professional domains. It provides a comprehensive overview of trends, methodological approaches, and data collection techniques in the field, making it a valuable resource for researchers, educators, and curriculum developers
Romance for Change: Diversity, Intersectionality and Affective Reparation in Contemporary Romantic Narratives: Primary and secondary sources
No full textPrimary and Secondary sources of the project "Romance for Change: Diversity, Intersectionality and Affective Reparation in Contemporary Romantic Narratives". The data includes the primary texts studied in the project as well as the secondary sources we used as our theoretical framework. The project focused on a corpus of post-millennial romantic novels produced by Anglophone female authors writing from marginalised or minoritised positions. In contrast to hegemonic romantic narratives, mostly featuring western and middle-class white women, these novels portray alternative and diverse romantic experiences of Indigenous or migrant women, of romantic heroines who are marginalised due to their sexuality, ethnicity, religious beliefs or cultural identity. The novels revise the hegemonic romantic discourses which have historically determined which relationships are acceptable, which subjects are represented as desirable, or who is endowed with the agency to love and to be loved. The novels rethink romantic relationships outside an exclusively white western and heteronormative framework, prompting reflections on the affective and intimate rights of these women. The corpus opens up the scope traditionally associated with the romantic novels dominating the contemporary global marketplace, and puts the romantic formula to political uses by connecting personal narratives to complex social realities. The novels were analysed from an intersectional perspective to show how these works expand the affective quest central to conventional romantic narratives to wider social issues, proposing more inclusive and egalitarian relational models and imagining narrative formulae conductive to collective forms of affective reparation and social change
Replication data for "Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells"
No full textThis dataset contains the raw and pre-processed microscopy images of hematopoietic stem cell (HSC) nuclei stained with DAPI described in the paper "Targeting RhoA activity rejuvenates aged haematopoietic stem cells" by Mejía-Ramírez et al. The code used to process and analyse the images can be found here: https://github.com/biomedical-data-science/hsc_rhoa. This dataset also contains the original Western Blot images. Moreover, it contains the code used to analyse the bulk ATAC, bulk RNA, and scRNA sequencing data, the raw count matrices and the main output obtained from running the code. The raw FASTQ files can be found under the GEO accession number GSE233989. The code is in an easily reproducible format, as bash and R scripts and Rmarkdown and Jupyter notebooks that contain comments that describe all the steps in the analysis. To reproduce it, download all the files in the repository directory "ATACandRNAseq_dataAnalysis_reproducibleCode" and, for each analysis, follow the steps in the "Readme.txt" file
Comparison of extraction methods
No full textComparison of two extraction methods (solid phase extraction -SPE- and dispesive liquid liquid microextraction -DLLME) for the determination of opioids and benzodiazepines in urine samples using LC-MS/MS.
Each extraction method was validated in terms of recovery and precision at different concentration levels. The files correspond to:
SPE: instrumental (1 file), validation at 5 ng/L (1 file), 20 ng/L (1 file) and 60 ng/L (1 file)
DLLME: instrumental (1 file), validation at 5 ng/L (1 file), 20 ng/L (1 file) and 60 ng/L (1 file
Exposure of contaminants in industrial areas
No full textThe data contains the chronic risk and hazard quotient of inhalation expressed in pg/m3 for different families of compounds in two different seasons, and considering the low and the high concentration of these families of compounds in air sample
On-line GC determination of contaminants
No full textThe data contain concentration of 1,3-butadiene, which is highly emmited in industrial petrochemical area, in air samples. The data is obtained after on-line monitoring using gas chromatography and the concentration is expressed in ng/m
Replication Data for: Are older teens more frustrated than younger teens by the covid-19 restrictions? The role of psychological maturity, personality traits, depression and life satisfaction
No full textThis dataset includes data from the published article " Are Older Teens More Frustrated than Younger Teens by the COVID-19 Restrictions? The Role of Psychological Maturity, Personality Traits, Depression and Life Satisfaction", published in the journal Current Psychology (https://doi.org/10.1007/s12144-023-04317-6). More especifically it includes data about 774 Spanish adolescents (60.9% women). The age range of the participants was 12 to 17 years. This sample was recruited online from April to May of 2021. The exclusion criteria for participating in this study were being under 12 years old or not providing informed consent. The data includes their ages, gender, and responses to the Psychological Maturity Assessment Scale (PSYMAS; Morales-Vives et al., 2012, 2013); Frustration associated with the pandemic (questionnaire developed ad-hoc for this study); Satisfaction With Life Scale (SWLS; Diener et al., 1985), and Beck Depression Inventory (BDI; Beck et al., 1961) questionnaires
Replication Data for: COmpliance with pandemic COmmands Scale (COCOS): The relationship between compliance with COVID-19 measures and sociodemographic and attitudinal variables
No full textThis dataset includes data from the published article " COmpliance with pandemic COmmands Scale (COCOS): The relationship between compliance with COVID-19 measures and sociodemographic and attitudinal variables", published in the journal Plos One (https://doi.org/10.1371/journal.pone.0262698). More especifically it includes data about 1410 individuals (59.2% women) who were resident in Spain. They were between 15 and 76 years old. This sample was recruited online from February to April 2021. The exclusion criteria for participating in this study were being under 15 years old, not resident in Spain, or not providing informed consent. The data includes their ages, gender, civil status, household incomes, if they have children, and responses to the COmpliance with pandemic COmmands Scale (COCOS questionnaire). It also includes data about the following issues: opinion about the usefulness of the preventive measures; feelings and opinions about the pandemic; health and vaccination; information channels, and optimism. (2025-05-22
Replication Data for: Tensing Flipper: Photosensitized Manipulation of Membrane Tension, Lipid Phase Separation, and Raft Protein Sorting in Biological Membranes
No full textMaterials:
Lipid solutions in CHCl3 of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-snglycero-3-phosphocholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Atto647N-labeled 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-Atto647N) was obtained from ATTO-TEC (Siegen, Germany), Cholesterol (Chol) ≥ 99% from Sigma-Aldrich (St. Louis, MO, USA), Neutravidin from ThermoFisher Scientific (Waltham, MA, USA).
Flipper-TR® (Flipper) was purchased from Spirochrome (Switzerland), DiIC18(5) solid (DiD) from Molecular Probes, Life Technologies Corporation (Carlsbad, CA, USA), and Halo-tagged JFX650 from Promega. Deuterated water (D2O), 9,10-Anthracenediyl-bis(methylene)dimalonic acid (ADMA), sodium azide (NaN3), polyvinyl alcohol (PVA, mw = 145 kDa), paraformaldehyde (PFA) and dithiothreitol were obtained from Sigma-Aldrich.
Ferrous ammonium sulfate hexahydrate >99% and xylenol orange (XO) sodium salt were purchased from Fischer Scientific (Hampton, NH, USA).
Ferrous Oxidation–Xylenol orange (FOX) assay for detection of lipid hydroperoxides
Flipper (2.5 μM) was added to lipid solutions of 50 μg/mL DOPC or DPPC in H2O, incubated in the dark for 15 min and then exposed to 488 nm light illumination for 2, 4 or 6 min (12 mW laser, Cobolt 06mld). FOX solution (final concentrations: 0.25 mM ferrous ammonium sulfate, 25 mM methanolic H2SO4 and 0.1 mM XO in water/methanol 25:75) was added to the lipid suspensions and the absorbance was recorded from 450 nm to 700 nm. Control samples of lipids and Flipper (columns dubbed dark in Figure 1b) were left in the dark for additional 45 min after the 15 min incubation period and then the FOX solution was added. Statistical analysis was performed using GraphPad Prism 10.2.2 (San Diego, CA, USA), P values less than 0.01 were considered statistically significant.
Singlet oxygen (1O2) detection
Lipid solutions of DOPC or DPPC at 50 μg/mL in D2O were incubated with Flipper (2.5 μM) for 15 min in the dark. ADMA (6 μM final concentration) was added and the solutions were illuminated for 30 seconds intervals with a 488 nm laser (12 mW, Cobolt 06-mld). The changes in the emission of ADMA were recorded (λex = 375 nm λobs = 390 nm – 500 nm) using a BioTek Synergy H1 microplate reader (Agilent Technologies, Santa Clara, CA, USA). In 1O2 quenching experiments, 10 mM NaN3 was added to the solutions prior to light irradiation. Control dark and light experiments were carried out, where lipid solutions incubated with Flipper were kept in the dark for the duration of the experiments and lipids without Flipper were exposed to the same light dose, respectively.
GUVs formation and imaging
GUVs were prepared following the PVA-assisted method with minor modifications.1 Glass coverslips were cleaned by 15 min of sonication in acetone followed by 15 min in alkaline detergent (Hellmanex). Then, 40 μL of PVA solution (5 % w/w) in milliQ water was spread on the coverslip and dried for 30 min at 50 °C. A total volume of 10 µL of the corresponding lipid at 1 mg/mL doped with 0.02 % biotinylated and 0.1 % fluorescently labeled lipids (only in two color experiments) in CHCl3 was spread on the PVA film and placed under vacuum for 1 h. The film was hydrated with 200 μL of 200 mM sucrose solution for 30 min at room temperature for DOPC and POPC, or 48 °C for DPPC and the ternary mixture POPC/DPPC/Chol. GUVs were collected using a cut pipette tip and diluted in 200 mM glucose solution, incubated with 1 µM Flipper (final concentration) for at least 15 min and imaged at room temperature in 18-well glass bottom dishes (Ibidi GmbH, Munich, Germany) previously coated with 50 μg/mL Neutravidin solution for 3 h at 37 °C or overnight at 4 °C.
Confocal and FLIM imaging
Confocal and lifetime images were obtained using a TCS SP8 microscope (Leica Microsystems GmbH, Germany) equipped with an HC PL APO CS2 60x/1.40 oil objective, a pulsed white light laser (WLL) operating at 20 MHz repetition rate, 70 % master power and a hybrid detector (HyD) in photoncounting mode and 16-bit depth. Flipper was excited at 488 nm and the emission was collected in the 530-650 nm range, except when imaging vesicles co-labeled with far-red dyes, where detection was narrowed to 530-630 nm. Samples containing Atto647N or DiD were excited at 650 nm and the emission was detected between 670-750 nm. Lifetime measurements and analysis were performed using LAS X software. The lifetimes were calculated by fitting a bi-exponential decay and values from the longest decay were used.2 Averaged lifetime images were obtained using the phasor plots. For phasor analysis, median or wavelet filters with a threshold of 5-8 were applied to better differentiate the photon clouds, which were then manually selected with linear or circular tools of radius 20-25.
Simultaneous FLIM and photo-oxidation experiments were performed at 488 nm by consecutive imaging scans with unidirectional exposure at 400 Hz and using 2x line accumulation, resulting in pixel dwell-times around 4-5 μs and a total time per frame of about 2.5 seconds. The power of the laser was typically set to 25 % of the master power, which resulted in 52 μW maximum power measured at the back of the objective using the bleaching point method provided from Leica, except when other powers were tested as indicated in the text (see Figure S2a). For dual-color imaging, sequential scan mode between lines was used.
Cell culture
HeLa cells were grown in DMEM medium at 37 °C and 5 % CO2. Transfection of Halo-tagged variants of GPI-AP and TfR was performed using the plasmids Str-KDEL_SBP-Halo-GPI and Str-KDEL_TfR-SBPHalo, respectively, which carry the so-called retention using selective hooks (RUSH) system.3 RUSH is based on the expression of streptavidin (Str) together with a targeting motif (in this case to the endoplasmic reticulum (ER) using the protein KDEL) and the reporter protein (GPI-AP or TfR) linked to a Str-binding peptide (SBP) motif. Once expressed, both proteins are retained in the ER until the addition of 40 μM D-biotin, which induces the displacement of SPB from Streptavidin and the release of the proteins. Cells were incubated at 37 °C for additional 60-90 min after biotin addition to ensure sufficient accumulation of the recombinant proteins at the plasma membrane and then incubated with the far-red Halo-JFX650 (1 μM) for 15 min. Cells were washed three times with warm PBS to remove the unbound dye.
Preparation of GPMVs
GPMVs were produced and isolated from HeLa cells as described in Sezgin et al.4 Cells were seeded on 35 mm glass bottom dishes (MatTek Corporation, Ashland, MA, USA) and grown for 24 h to a confluency of around 70 %. To induce the formation of non-phase separated GPMVs, cells were washed twice with GPMV buffer (150 mM NaCl, 10 mM HEPES, 2 mM CaCl2 at pH 7.4) and incubated with 25 mM PFA and 2 mM dithiothreitol for 2 h at 37 °C. Supernatants were collected and centrifuged for 90 seconds at 1000 g to remove detached cells. GPMVs were allowed to settle for 1 h and 20 l from the bottom of the tube were diluted in 130 L of GPMV buffer, incubated with 1 µM Flipper (final concentration) for at least 15 min and transferred to 18-well glass bottom chambers previously coated with 2 mg/ml BSA