Taiwan Agricultural Research Institute Council of Agriculture, Executive Yuan
Taiwan Agricultural Research Institute Institutional RepositoryNot a member yet
10534 research outputs found
Sort by
Development of a Selective Medium for Detecting Ganoderma lucidum in Compost
No full text本研究主要目的在於研發靈芝菌的選擇性培養基，以便偵測廢棄的蕈菌生長基質調製成之堆肥或介質中是否仍有靈芝菌的存活。西元2009年，由嘉義取得的靈芝［Ganoderma lucidum （Leyss. ex Fr.）Karst.］太空包基質中分離GL1與GL3兩株靈芝菌菌株，進而評估七種真菌培養基、十種殺菌劑及兩種抗生素等對兩靈芝菌菌絲生長的影響，藉以研發靈芝菌的選擇性培養基。靈芝菌選擇性培養基（GLS）係利用10% V-8培養基作為基礎基質，並添加2 gL^(-1)腐絕（40% ai）、200 mg L^(-1)滅達樂（35% ai）、200 mg L^(-1)鏈黴素、200 mg L^(-1)氯黴素、1 mg L^(-1)五氯硝基苯（75% ai）、1.25 g L^(-1)單寧酸、10 mlL^(-1)酒精（95% v/v）及2 ml L^(-1)乳酸（85% v/v）配製而成。靈芝菌在GLS培養基生長2-3天可看到白色菌落，且於菌落周圍出現褐色反應。利用多重土丸取樣法分析含有不同靈芝接種源濃度之太空包木屑基質，結果顯示由台中及嘉義取得之靈芝太空包基質以無菌之太空包基質稀釋1000倍後，GLS培養基仍可偵測到G. lucidum的存活。本研究證明GLS培養基，除可自廢棄已久的太空包基質成功分離到靈芝菌外，尚可有效抑制其他雜菌的生長。
The purpose of this study was to develop a selective medium for detecting Ling-Chih, Ganoderma lucidum (Leyss. ex Fr.) Karst. in compost. In 2009, two isolates GL1 and GL3 of G. lucidum acquired from a Chiayi mushroom cultivation field were used to conduct the research. GLS (Ganoderma lucidum selective) medium was developed by amendment of 10% V-8 vegetable juice agar (1 L) with 2 g thiabendazole (40% ai), 200 mg metalaxyl (35% ai), 200 mg streptomycin sulfate, 200 mg chloramphenicol, 1 mg PCNB (75% ai), 1.25 g tannic acid, 10 ml ethanol (95% v/v), and 2 ml lactic acid (85% v/v). The GLS medium rapidly detected G. lucidum in the compost within 2-3 days. Amendment of the medium with tannic acid induced G. lucidum to produce brown halo surrounding the colony as a selective feature. The multiple-pellet soil-sampler method was used to evaluate the sensitivity of GLS medium. The GLS medium was able to detect G. lucidum in the spent sawdust substrate which were 1000-fold diluted in disinfected sawdust substrate. This study concluded that the GLS medium could detect the survival of G. lucidum in the compost, and inhibit the growth of other fungi. Thus, GLS medium was recommended for manufacturers to determine if the compost is a suitable fertilizer
Effects of Pre-harvest Water Management on Losses in fruit during storage of 'Ponkan' Mandarin (Citrus reticulata Blanco)
No full text為探討採收前斷水處理對' 椪柑' 貯藏期間果實損耗情形之影響，連續二年進行果樹於採收前56 天、28 天、14 天、7 天及 3 天停止補充灌溉之試驗。二供試年期均顯示採收前3 天才斷水植株之果實，較採收前56 天即斷水者，其果實失重率較高，且綠蒂率減少較快。2005 年期採收前3 天才斷水者，其所結果實浮皮率較高；2006 年期接近採收前才斷水的植株，其果實腐爛率顯著高於採收前28 天即已斷水者。
To study the effect of pre-harvest water management on the deterioration in 'Ponkan' mandarin during storage, irrigation was ceased 56, 28, 14, 7 and 3 days prior to harvest for two consecutive years. Fruit harvested from trees subjected to cessation of water supply 3 days prior to harvest showed greater weight loss and a greater decrease in the rate of green button than those picked from trees subjected to cessation of water supply 56 days prior to harvest for the two years. The former also displayed a higher percentage of peel puffing in 2005. In addition, fruits from all the groups stopping the supply of water too close to harvesting exhibited a higher decay percentage compared with fruits treated with withholding water for 28 days before harvesting in 2006
