Journal of Experimental Biology and Agricultural Sciences
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Evaluation of Phytochemical and Antibacterial properties of leaf extract of Cinnamomum tamala oil
Get PDFCinnamomum tamala, commonly known as tej patta is widely used as a spice in Indian cuisine for its aroma and flavoring property as the leaves contain essential oil. The essential oil is extracted with the help of the Clevenger apparatus using dry leaves. This oil is mainly used as medicine for releasing gas as well as a carminative agent and diuretic agent. It also improves the digestive system and helps in increasing appetite. This study aimed to determine the phytochemical properties and antibacterial potential of different extracts (aqueous, methanol, and acetone) and oil of C. tamala leaves. The phytochemical evaluation shows the presence and absence of alkaloids, flavonoids, phenolic compounds, steroids, tannins, glycosides, terpenoids, saponins, proteins, and carbohydrates in the aqueous, methanol, and acetone extracts. The efficacy of antibacterial properties of prepared extracts was examined against E. coli and Salmonella typhi (gram-negative bacteria) and Staphylococcus aureus and Bacillus subtilis (gram-positive bacteria). These bacterial cultures were obtained from IMTech Chandigarh. From the results of the antibacterial study it has become evident that among three extracts, the maximum zone of inhibition was obtained in the aqueous extract which was followed by methanolic and acetone extract. With the help of a Clevenger apparatus, Bay leaf oil was extracted to establish antibacterial properties. Henceforth, to analyze the antibacterial potential of the oil sample, the test was performed against the mentioned bacterial species (E. coli, Salmonella typhi, Staphylococcus aureus, and Bacillus subtilis) and reported significant antibacterial activities. From the outcome of this study, it has become clear that Bay leaf oil has potent antibacterial properties against selected bacterial species
Disease Complication in a Geriatric Pig-Tailed Macaque (Macaca nemestrina) reported from Wildlife Rescue Centre (WRC) Jogja – Case Study
Get PDFIn the current study, a geriatric male pig-tailed macaque having a mass growing up to the left shoulder for the past five months has been reported from the Wildlife Rescue Centre (WRC) Jogja. It was an approximately 4x4x2 cm, firm, hairless focal non-encapsulated mass suspected as a skin-derivative tumor. A cytological test was performed on the mass and a skin biopsy was carried out to see the tissue content of the mass. Histopathological examination confirmed that the mass was identical to papilloma. In the next step of the study, the surgery was conducted and the mass was removed. However, after surgery, the health condition of the macaque gradually deteriorated and the performed hematological tests revealed a gradual increase in the number of leukocyte cells that indicated a developing chronic inflammatory response. Further, the erythrocyte level was in the normal range but showed a declining trend which suspecting progressing anemia. One month after the surgery general muscle stiffness was reported in the macaque which is a clinical sign of tetanus and the macaque died even after the ATT serum treatments. Necropsy findings confirmed a significant chronic bilateral diffuse pneumonia. This case study showed an unpredicted sequence of disease occurrence in geriatric macaque after undergoing the initial surgery of cutaneous mass extraction. Hence, the results of the study suggest that a routine regular health screening coupled with clinical laboratory examination may assist the early detection based on the subclinical signs of disease in geriatric animals in rehabilitation centers
Descriptive Comparison of Gastrointestinal Tract Histology of Various Avian Species based on their Natural Diet
Get PDFMany animals species develop their gastrointestinal tube with special features to accommodate their natural diet to survive under adverse conditions including the nutrient absorption capability. Information related to the histologic description of various bird species' digestive organs based on their diet and its significance is yet limited. This study aimed to present a descriptive explanation of gastrointestinal organs of a changeable hawk-eagle (Nisaetus cirrhatu) and oriental honey buzzard (Pernis ptilorhynchus) as carnivorous, a southern cassowary (Casuarius casuarius) as an omnivorous, and a domestic chicken (Gallus gallus) as granivorous. In the current study, proventriculus (glandular stomach) and intestinal segments were microscopically examined and compared to understand the special histological features among avian species due to their important roles to digest the ingesta. The dissected specimens were preserved in 10% non-buffered formalin, then were processed through the common standard procedure of tissue processing and eventually stained with haematoxylin-eosin. Microscopic observation showed variation in shape and size of proventricular glandular architecture among raptors. The intestine muscular layer of the cassowary also showed distinct thickness among birds. These results of the study preliminary proved that variation in diet might affect the histologic features of avian gastrointestinal tracts
Effect of the combined application of Lampung Robusta Coffee Extract and Lactobacillus acidophilus on the Ileum and Caecum Histopathology in Salmonella enterica infected Balb/C Mice
Get PDFSalmonella enterica is a gram-negative bacterium that can cause Salmonellosis and gastroenteritis in humans and animals. Further, this bacterial infection is also associated with the reactive oxygen species (ROS) by lipid peroxidase that can destroy the intestinal cell's membrane. This study aimed to evaluate the preventive effect of the combined application of Lampung Robusta coffee extract and Lactobacillus acidophilus on the Ileum and Caecum Histopathology in Salmonella enterica infected Mice. In this study, male Balb-c mice aged between 8-10 weeks and weight 20-25 grams were used, these experimental animals were divided into six experimental groups namely K- (Negative control without any infection), K+ (Positive control with S. enterica), KL (Only L. acidophilus treated mice), P1, P2, and P3 were given a preventive extract of coffee with a concentration of 250 mg/kg BW, 500 mg/kg BW, and 750 mg/kg BW respectively and L. acidophilus to S.enterica infected mice and arrange in completely Randomized Design. Descriptive histopathological analyses were carried out after HE staining and villi's length and width for ileum's histopathology and counting goblet cells for caecum's histopathology was scored. The results of the study revealed that administration of Robusta Coffee extract @ 250 mg/ kg BW and L. acidophilus has a preventive effect on the ileum and caecum damage caused by salmonellosis
Potential COVID -19 Therapeutics in Clinical Trials – A Brief Review
Get PDFThe severe acute respiratory syndrome coronavirus 2 (SARS – CoV2), the causative viral pathogen of the COVID-19 pandemic belongs to the family of Coronaviruses which are positive single stranded RNA viruses. The scientific fraternity has developed and developing various types of vaccines for prevention against COVID-19, such as inactivated virus vaccines, mRNA vaccines, replicating vector protein subunit vaccines, etc., Out of which ten vaccines namely Novovax, Covovax (protein subunit vaccines), Pfizer BNT16b2, Moderna mRNA 1273 (mRNA vaccines), Johnson & Johnson Ad26, Cov2.S, Astrazeneca AZD1222, Covishield (non-replicating viral vector vaccines), Covaxin, Sinopharm BBIBP-CorV, CoronoVac (inactivated vaccines) have been approved for clinical use by WHO. There is an urgent need for SARS-CoV2 specific therapeutics for the treatment of COVID-19 as there is the emergence of various variants such as Alpha, Beta, Gamma, Delta, Omicron, etc. The emergence of variants that possesses immune evading property and spike protein mutation have increased infectivity and more pathogenicity which impelled the need to develop various therapeutics for the treatment of COVID-19. This review compiles the information about potential antiviral candidates in preclinical trials intended for the treatment of COVID-19. The clinical development of such antivirals will be very crucial for the treatment of COVID-19 and also to curb the spread as the present scenario depends on the development of effective prophylactic vaccines
Studies on NF-κB Docking with Common Bioactive Compounds in Punica granatum peel and Vitis vinifera Seeds
Get PDFPlant-based products have long been utilized as traditional remedies throughout the world. Higher plants serve as a "reservoir" of phytochemicals known as bioactive compounds, which are used as valuable medicines to fight a variety of diseases across the world. The materials that are considered waste in plants possess bioactive components with potential medicinal properties due to the presence of important secondary metabolites known as phytochemicals. In this study, the interaction of phytochemicals that are present in both Punica granatum peel and Viti vinifera seeds was analyzed on protein NF-κB. Compounds 2,3- dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP), α-tocopherol-β-D-mannoside, gamma-sitosterol, glycerine, guanidine, pyrogallol, palmitic acid, and ethyl palmitate were the eight phytoconstituents which are present in both the selected plant materials and further investigated for in-silico analysis. The 3D protein structure of NF-κB was retrieved from the protein data bank. The structures of bioactive compounds were obtained from Chemspider and drawn using Chemsketch software. This study clearly shows that α-tocopherol-β.-D-mannoside interacts with target protein NF-κB with an energy level of -10.88 kcal/mol (2 hydrogen bonds). The interaction of α-tocopherol-β-D-mannoside with NF-κB may play a major role in anti-oxidant and anti-cancer potential and provide chemopreventive property for both P. granatum peel and V. vinifera seeds
Screening, identification, and antibiotic activity of secondary metabolites of Penicillium sp. LPB2019K3-2 isolated from endemic amphipods of Lake Baikal
Get PDFThis study aimed to assess the influence of nutrient media content on the production of antibiotics and the ability of water fungi isolated from lake Baikal to synthesize novel natural products. Interest in this topic stems from the high demand for new drugs, and studies are carried out via the screening of new natural products with biological activity produced by unstudied or extremophilic microorganisms. For this study, a strain of Penicillium sp. was isolated from endemic Baikal phytophagous amphipod species. Here, we identified natural products using the following classical assays: biotechnological cultivation, MALDI identification of the strain, natural product extraction, antimicrobial activity determination, and modern methods such as HPLC-MS for the dereplication and description of natural products. It was found that many detected metabolites were not included in the most extensive database. Most of the identified metabolites were characterized by their biological activity and demonstrated antibiotic activity against model Gram-positive and Gram-negative bacteria. The isolated strain of water fungus produced penicolinate B, meleagrin A, austinoneol A, andrastin A, and other natural products. Additionally, we show that the synthesis of low-molecular-weight natural products depends on the composition of the microbiological nutrient media used for cultivation. Thus, although the golden age of antibiotics ended many years ago and microscopic fungi are well studied producers of known antibiotics, the water fungi of the Lake Baikal ecosystem possess great potential in the search for new natural products for the development of new drugs. These natural products can become new pharmaceuticals and can be used in therapy to treat new diseases such as SARS, MERS, H5N1, etc
Essential oils as valuable feed additive: A narrative review of the state of knowledge about their beneficial health applications and enhancement of production performances in poultry
Get PDFNew research has begun to develop safe and effective alternatives to feed-antibiotics as growth enhancers in response to mounting pressure on the poultry sector to do so. There is a significant demand for poultry products all across the world right now. To achieve this goal, key performance indicators are optimized, such as the rate of chicken growth, the amount of feed used, and the health of the flock as a whole. As a result of this growing need, various alternatives to antibiotics have entered the market. New approaches are desperately needed to keep poultry productivity and efficiency at a high level in the face of mounting pressure to limit the use of antibiotics. Recent years have seen an uptick in interest in the potential of aromatic plant extracts as growth and health boosters in poultry. The great majority of plants' positive effects are accounted for by essential oils (EOs) and other secondary metabolites. EOs have been proven to promote digestive secretion production, improve blood circulation, exert antioxidant qualities, reduce levels of dangerous microbes, and maybe improve the immune status of poultry. EOs are often believed to be safe, non-toxic alternatives because they are all-natural, chemical-free, and devoid of potentially harmful deposits. EOs are extracted from plants, and while there are thousands of them, only approximately 300 have been deemed to have significant commercial value. Many different types of bacteria, viruses, fungi, and parasites are negatively affected by EOs in multiple studies conducted both in vitro and in vivo. The review covers the fundamentals of EOs, their anti-oxidant and immunomodulatory capabilities, their growth-promoting benefits, and their effectiveness against numerous diseases in poultry
Pre-Harvest Sprouting Tolerance in 36 Bread Wheat Genotypes
Get PDFPre-harvest sprouting (PHS), promoted by rainfall during crop maturity, is a high problem in many wheat-producing regions of the world. Considering its importance in Brazil, 36 national and international varieties and advanced lines of wheat were evaluated for their tolerance to PHS. For this purpose, two experiments were conducted over three years. Seed pericarp rupture was used as an indicator of the beginning of germination. The data were analyzed using analysis of variance, the Scott-Knott test, and the Lin and Binns method. The wide range of germination percentage values allowed the genotypes to be classified as tolerant (in experiment 1 - ND 674 and Grandin*2/RL 4137 and experiment 2 - Frontana and Grandin) and moderately tolerant (Alsen, CD 114, and Milan/3/Attila//Fang 69/CIMMYT 3 in Experiment 1; Avante, BRS 177, IAC 5-Maringá, Onix, OR 1, RL 4137, and Rubi in Experiment 2). Because tolerance to PHS is under genetic control and can be improved through breeding programs, the challenge for wheat breeders is to combine increased PHS tolerance with other requirements to meet market demands
An intensive study on pesticides contamination and its removal in fruits and vegetables collected from Ghaziabad, India
Get PDFThese days pesticides are extensively applied in agriculture to increase productivity; although these pesticides increase productivity but also have a negative impact on the consumer. Thus, pesticide exposure in agricultural products must be decreased. The present study attempted to assess pesticide residues in samples of apple, tomato, and brinjal and determine the efficacy of washing solutions in pesticide removal. For sample preparation, the QuEChERS method was employed, and prepared samples were analyzed using gas chromatography-mass spectrometry. Results of the study revealed that among the collected samples, 58.33 percent samples were showing lower pesticide residues as compared to the maximum residue limit (MRL) while 12.5 percent of the samples were showing higher pesticides residues as compared to the suggested MRL. Further, from the collected fruits and vegetable samples, the presence of the chlorantraniliprole, carbendazim, beta endosulfan, chlorpyrifos, malathion, carbaryl, thiomethoxam, DDT, and flubendiamide were detected in the range of 0.0–1.41 mg/kg. Among the detected pesticides, chlorpyrifos and flubendiamide were the most commonly detected pesticides. Effectiveness of different washing solutions was studied, which indicated a significant reduction in residues of all the washing solutions compared with the control (p < 0.05) and concluded that ascorbic acid and sodium bicarbonate solution was very effective in pesticides removal compared with water and chemical alone