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Managing Biological Risks in Biomedical laboratories of Public Hospitals in Athens, Greece, based on the Biosafety requirements
Get PDFObjectives: The aim of the present study was to review the laboratory workers’ perception on the biological risks in Biomedical laboratories of Public Hospitals in Athens, Greece, to evaluate how they are managing the biological materials and to propose mitigation measures according to the existing risk, the local legislations and the international Biosafety guidelines.
Materials and Methods: The study was designed as a cross-sectional study with a detailed health and safety (H&S) questionnaire focused on biosafety and biorisk management. A total of 36 biomedical laboratories Biosafety Level-2 in 20 public hospitals were assessed for their biosafety containment and compliance with biosafety practices. Laboratory staff (medical laboratory doctors, medical laboratory technologists, laboratory assistants, biologists and biochemists) (n = 415) completed the questionnaire.
Results: The results showed, that a significant percentage of laboratories lacked proper management of the biological agents and biological materials in general, thus more specific: restricted access 48.9%, controlled and independent ventilation 36.6%, use of BSCs 31.8%, biorisk management system in place 31.6%, risk assessments 28.4%, biosafety manuals 21.4%, SOPs 35.9%, assigned biosafety officers 10.8%, occupational Doctor 34.9%, accidents reporting 25.5%, emergencies plan 34.2% and biosafety training programs 28.2%.
Conclusion: There are marked deficiencies in containment and administrative controls, as well as in the implementation of the Greek and EU biosafety legislation. This emphasize the urgency of addressing critical gaps in biosafety and in emergency preparedness in Greek biomedical laboratories. Therefore a Biorisk Management System, risk assessments, SOPs, assignment of a Biosafety Officer, staff trainings and emergency response plans should be developed, applied and enforced, in compliance with the local and European legislation and guidelines
Intra-abdominal metastasis after pelvic primary Synovial Sarcoma resection: case report
Get PDFIntroduction: Synovial sarcoma is a malignant soft tissue sarcoma. It occurs predominantly in young adults. 90% of synovial sarcomas occur in the extremities, they have been reported in other sites, including abdomen and pelvis. Up to 40% of all patients with soft tissue sarcomas develop distant metastasis, despite adequate local disease control.
Aim of the article: To provide an update on one of the rarest and most aggressive tumors, to affirm the importance of early management and especially surveillance, and to encourage the codification and the management of these tumors.
Presentation of cases: Here, we discuss a case of a 78-year-old female patient, operated 10 years earlier for a pelvic Synovial sarcoma, who presented with intra-abdominal mass to our hospital that eventually turned out to be a metastatic intra-abdominal synovial sarcoma following biopsy.
Conclusion: It is important to be aware that primary intraabdominal tumors can occur and delayed metastasis is more typical of synovial sarcoma than of many other sarcomas and should be considered in determining the appropriate frequency and duration of follow-up imaging
Indoor air quality in selected school buildings in the Central Sector of Athens at the Attica’s Region and potential Health Risks
Get PDFAims and scope: Indoor air pollution is considered as an important environmental risk factor for health. Indoor air quality in schools is very important, as students and teachers spend most of their day (30%) indoors and consequently are more exposed to indoor pollution than outdoor air pollution. The present study has the aim to investigate the indoor air quality (IAQ) in school buildings in the Central Sector of Athens at the Attica’s Region and record physical parameters and concentration levels of indoor air pollutants that are associated with comfort, health and safety conditions inside the classrooms. Methods: The indoor air quality research was conducted in forty-seven (47) classrooms in a total of twenty-six (26) school buildings in the Central Sector of Athens at the Attica’s Region, during the period from March 2022 to May 2023. The air pollutants Carbon dioxide (CO2), Carbon monoxide (CO), Volatile Organic Compounds (VOC’s), Nitrogen dioxide (NO2), Particulate matters PM (PM10, PM2.5) and physical parameters such as temperature (T) and relative humidity (RH) were monitored by the series 500 Portable Air Quality Monitor AeroQual, during (1) teaching hour per day in each classroom. During the samplings some windows and doors were opened, due to measures and recommendations for health and safety for students and teachers against COVID-19. Findings: The overall mean concentrations of the main parameters recorded inside the schools were 0,136 ppm CO, 823,38 ppm CO2, 12,07 ppm VOC’S, 0,006 ppm NO2, 38,1 μg/m3 PM10 and 15,4 μg/m3 PM2.5. The mean recorder temperature was 24,52 oC, and relative humidity was 45,78%. In this study a total number of twenty- two (22) classrooms (46,8%) of schools at the Attica’s Region had no comfort temperatures for students. In all cases indoor CO concentrations were below the 50 ppm, guideline set by WHO. Eight (8) of the forty-seven classrooms in the Region of Attica (17%) had a CO2 concentration more than 1000ppm. VOC’s exceeded the limit value of 0,8ppm indoors in all schools (100%). There was statistically difference for CO, CO2, ΝΟ2 (p<0,001), for VOC’s (p=0,004) and for PM10 (p=0,028) between indoor and ambient air. Conclusion: The indoor air quality of the classrooms was influenced by the outdoor air, the location of school, the number of windows that were opened during the lesson, the number of students inside the classroom, the activities, furnishing and school equipment. No comfort conditions in classrooms and exceeded limits of indoor air pollutants can lead to diminished IAQ and thereby harmful effects on students. A well airing of the classrooms during the lessons and breaks is necessary for a better air quality. Ventilation is one of the most important factors affecting indoor air quality, diluting the exposure agents originating from indoors
Evidence of komatiitic basalt enclaves in the Téra-Ayorou pluton (Liptako, West Niger) (West African Craton)
Get PDFThe present study focuses on the basic enclaves (amphibolo-pyroxenites) of the Téra-Ayorou pluton in Niger Liptako (NE portion of the Man Ridge of the West African Craton). The methodology used includes field observations, supported by polarizing microscope observations of thin sections and geochemical analyses of whole rock. These enclaves are characterized by high MgO, low Na2O, K2O, and TiO2 contents, high CaO/Al2O3 ratios, depletion of light rare earth and enrichment in Ni and Cr. These basic enclaves are thought to come from certain basic to ultrabasic Pogwa and Ladanka plutonites in the Diagorou-Darbani greenstone belt, with which they share the same geochemical characteristics. This suggests that these enclaves were ripped out by the pluton as it was being emplaced. The basic enclaves and basic plutonites with ultrabasites have different signatures from those of the birimian basites of the West African Craton, which are tholeiitic and calc-alkaline. The amphibolo-pyroxenite enclaves of the Téra-Ayorou pluton and the basic to ultrabasic plutonites of the Diagorou-Darbani greenstone belt constitute a fairly continuous line of komatiitic rocks from peridotites (serpentinites) to basalts (metapyroxenites, amphibolites). This komatiitic lineage results from the fractional crystallization of a magmatic liquid from a mantle source with variable partial melting rates. The komatiitic line and the tholeiiti
Acquisition des notions de la circulation sanguine par les élèves au Collège
Get PDFFréquemment, la compréhension du concept du système circulatoire pose des problèmes, car il combine d'autres concepts biologiques tels que la nutrition et la respiration. Cette étude vise à évaluer si les élèves de la troisième année du collège parviennent à saisir ce concept à la fin du module, après avoir étudié l'alimentation et la respiration, et mettre en évidence les principaux défis liés à l'enseignement-apprentissage de la circulation sanguine au collège. Il s’agit d’une étude exploratoire et descriptive, Notre population est composé de 100 élèves du collège. Nos résultats révèlent que les apprenantes et apprenants ont des problèmes d’acquisition à des degrés variables. Ainsi, les pourcentages des difficultés rencontrés, pour établir la distinction entre les veines et les artères (86 %), l'ouverture ou la fermeture du modèle de circuit sanguin (64 %), ainsi que la conception du cœur en tant que pompe ou en tant que double pompe (64 %), les organes et les fonctions du système circulatoire ainsi que les composants du système circulatoire(32% ). En fait de nombreux élèves interrogés négligent la réponse sur les risques qui menacent le système circulatoire, La confusion entre le système circulatoire et le système respiratoire a enfin été observée. Il est aussi primordial de mettre l'accent sur toutes les idées et les obstacles rencontrés par Les élèves afin d'améliorer la qualité d’acquisition des notions de la circulation sanguine.
Frequently, the concept of circulatory system is difficult to understand since it brings together other biological concepts such as nutrition and respiration. This study aims to determine whether students in the third year of middle school manage to understand this concept at the end of the module, and to highlight the main challenges related to the teaching-learning of blood circulation in middle school as well as to describe all the ideas and obstacles encountered by the students involved. This is an exploratory and descriptive study. Our population is made up of 100 middle school students. Our results reveal that learners have acquisition problems to varying degrees. Thus, the percentages of difficulties encountered, in establishing the distinction between veins and arteries (86%), the opening or closing of the blood circuit model (64%), as well as the conception of the heart as a pump or as a double pump (64%), the organs and functions of the circulatory system as well as the components of the circulatory system (32%). In fact, many students interviewed neglect the response to the risks that threaten the circulatory system. The confusion between the circulatory system and the respiratory system has finally been observed. It is also essential to emphasize all the ideas and obstacles encountered by students in order to improve the quality of acquisition of the concepts of blood circulation
La remise en cause du contrôle de pleine juridiction par la procédure en révision des jugements en droit burundais
Get PDFPar l’effet de circonstances extraordinaires, la révision permet de demander au juge, dans des cas limités, à réexaminer une décision définitive. L’équité procédurale recommande que ce recours respecte les garanties d’indépendance et d’impartialité. Ces dernières permettent de trancher, d’exercer le contrôle de pleine juridiction, sans aucune limite, ni influence et ingérence des forces extérieures. Pour répondre convenablement à sa mission, le juge doit examiner tous les aspects litigieux aussi bien sur la forme que sur le fond. Néanmoins, les articles 163 et 171 de la loi organique n°1/21 du 3 août 2019 freinent l’exercice effectif du contrôle de pleine juridiction. Ces dispositions véhiculent une injonction qui oblige le magistrat du siège à statuer au fond sans analyser les conditions de recevabilité inhérentes à la forme. Compte tenu des avancées de la jurisprudence internationale dans l'encadrement du procès équitable, cette injonction empêchant d’exercer un contrôle judiciaire exhaustif constitue une incohérence qui remet en question la qualité de cour ou de tribunal à cette juridiction suprême. Il apparaît plus qu'indispensable pour le droit positif burundais d’aller vers une « culture de séparation des pouvoirs », confiant la mission de rendre la justice uniquement aux cours et tribunaux, valorisant les garanties procédurales et institutionnelles contenues dans la Constitution.
As an extraordinary judicial procedure, revision aims to ask the judge, in limited cases, to re-examine a final decision, which has become res judicata, due to new elements. In the course of the trial, the guarantees of independence and impartiality promote the proper application of the law and the fairness of judicial proceedings. Those guarantees help the judge to handle contentious cases without any influence and interference from external forces. While accomplishing its mission, the judge must examine all the contentious aspects, both in form and in substance. However, the Supreme Court’s proceeding doesn’t allow the analysis of the admissibility of the appeal for review. The judge's competence to review the full jurisdiction is called into question by articles 163 and 171 of the Supreme Court’s law. These articles convey an injunction which obliges to judge on the merits without analyzing the conditions of admissibility. According to the international case law, this inconsistency calls into question the quality of court at this supreme jurisdiction by preventing it from exercising effective judicial control. According to the advances of the international case law in the framework of a fair trial, it seems more than essential for Burundian positive law to move towards a "culture of separation of powers" by entrusting the mission to dispense justice only to the courts and tribunals, by promoting the procedural and institutional guarantees contained in the Constitution and in the international instruments ratified by Burundi
Conductas Sexuales de Riesgo en Jóvenes Universitarios
Get PDFIntroducción: Las conductas sexuales de riesgo se han vuelto cotidianas sobre todo en los jóvenes; esto genera un problema de salud pública en el que se compromete su salud sexual. Objetivo: Describir la prevalencia de las conductas sexuales de riesgo en jóvenes universitarios según se curse o no, alguna carrera del área de la salud dentro de Querétaro. Métodos: Estudio cuantitativo, transversal y descriptivo, muestreo por conveniencia de 85 jóvenes universitarios, de las carreras de Enfermería e Ingeniería Civil. Se aplicó el cuestionario de Conducta Sexual Segura (CSS) mediante un Formulario de Google. La duración del estudio fue de aproximadamente 14 meses a partir de mayo del 2021. Se utilizaron medidas de tendencia central y el estudio se elaboró con base en la Ley General de Salud. Resultados: De manera global, el 44.7% de los estudiantes a veces tienen relaciones sexuales sin usar condón y el 5.9% siempre. Se identificó que más de la mitad de los alumnos (50.6%) toma bebidas alcohólicas antes de una relación sexual. 45.7 % de los alumnos de ingeniería tiene sexo oral sin uso del preservativo, en contraste con 22% de enfermería y tienen relaciones sexuales sin condón, algunas veces, el 48.5 % los alumnos de ingeniería y 42 % de enfermería. Conclusión: Las conductas sexuales de riesgo son un problema aún presente en la actualidad. Se identificó una alta prevalencia en ambas carreras, siendo las de mayor importancia la falta de uso de barreras protectoras y el consumo de sustancias previo a la relación sexual.
Introduction: Risky sexual behaviors have become a daily occurrence especially in young people; this generates a public health problem in which their sexual health is compromised. Objective: To describe the prevalence of sexual risk behaviors in young university students according to their health careers or not, in Queretaro. Methods: Qualitative, cross-sectional and descriptive study, convenience sampling of 85 university students, studying nursing and civil engineering. The Safe Sexual Behavior (CSS) questionnaire was applied using a Google Form. The duration of the study was approximately 14 months from May 2021. Central tendency measures were used and the study was developed based on the General Health Law. Results: 44.7% of the students sometimes have sex without using a condom and 5.9% always. It was identified that more than half of the students (50.6%) drink alcoholic beverages before sexual intercourse. 44.7 % of the engineering students have oral sex without condom, and 22 % nursing students. Have sex without condom, sometimes, 48.5 % engineering students and 42 % nursing students. Conclusion: Risky sexual behaviors are a problem still present today. A high prevalence was identified in both careers, the most important being the lack of use of protective barriers and the consumption of substances prior to sexual intercourse
Formes topographiques des arthroses des membres en consultation rhumatologique à Abidjan
Get PDFObjectif: Déterminer les formes topographiques des arthroses des membres en rhumatologie à Abidjan. Méthodologie: Etude transversale réalisée dans le service de rhumatologie du CHU de Cocody à Abidjan (Côte d’Ivoire) sur une période allant du 1er Février au 10 Mai 2023. Les patients venus en consultation de rhumatologie présentant des arthralgies mécaniques des membres et disposant des imageries ont été inclus. Le diagnostic était posé par le médecin sur la base des arguments cliniques et paracliniques. Les critères de Kellgren et Lawrence avaient permis la classification des stades radiologiques de l’arthrose des membres. Nous nous sommes intéressés aux données sociodémographiques, cliniques et paracliniques. Résultats: Deux cent quatre-vingt-six patients ont été recensés pendant la période d’étude. L’âge moyen était de 55 ans ± 11 ans et le sex ratio de 0,42. La principale catégorie socio-professionnelle était le secteur informel (34,96%). La majorité des patients avait un niveau socio-économique bas (80,2%) et vivait en milieu urbain (92,06%). Les antécédents les plus retrouvés étaient l’hypertension (33,21%) et l’ulcère gastro-duodénal (17,13%). Les patients étaient obèses à 68,18%. La durée moyenne d’évolution des symptômes jusqu’au diagnostic était de 11 mois. On retrouvait 185 localisations d’arthrose aux membres inférieurs (64,68%) et de 101 localisations aux membres supérieurs (35,31%). Les formes topographiques observées aux membres inférieurs incluaient : gonarthrose 163 (88,10%) ; coxarthrose 14 (7,56%); arthrose de la cheville 7 (3,78%), arthrose du mediopied (1,08%). Aux membres supérieurs, les localisations observées étaient les suivantes : arthrose de l’épaule: 21 (20,79%) ; arthrose digitale: 67 (66,33%), arthrose du coude 7 (6,93%), arthrose du poignet 9(8,91%). Conclusion : L’arthrose des membres touche les femmes obèses et domine aux membres inférieurs. Le genou reste sa localisation la plus fréquente.
Objective: Determine the topographies of osteoarthritis of the limbs in patients seen in rheumatology in Abidjan. Methodology: Cross-sectional study was conducted in the rheumatology department of the Cocody University Hospital in Abidjan (Ivory Coast) from 1st February to 10 May 2023. Patients who came for a rheumatology consultation presenting with mechanical arthralgia of the limbs and with imagery were included. The diagnosis was made by the medical doctor on the basis of clinical and paraclinical arguments. The Kellgren and Lawrence criteria allowed the classification of the radiological stages of osteoarthritis of the limbs. We were interested in sociodemographic, clinical and paraclinical data. Results: Two hundred and eighty-six patients were enrolled during the study period. The mean age of patients was 55 years ± 11 years and the sex ratio was 0,42. The dominant socio-professional category was the informal sector (34.96%). The majority of patients had a low socio-economic level (80.2%) and lived in urban area (92.06%). The most common antecedents were hypertension (33.21%) and peptic ulcer (17.13%). The patients were 68.18% obese. The average duration of symptom progression until diagnosis was 11 months. There were 185 localizations of osteoarthritis in the lower limbs (64.68%) and 101 localizations in the upper limbs (35.31%). The different topographies in the lower limbs included: knees 163 (88.10%); hips 14 (7.56%); osteoarthritis of the ankle 7 (3.78%), osteoarthritis of the midfoot (1.08%). For the upper limbs, the localizations observed were as follows : digital osteoarthritis 67 (66.33%), osteoarthritis of shoulder 21 (20.79%), osteoarthritis of elbow 7 (6.93%), osteoarthritis of wrist 9 (8.91%). Conclusion: Osteoarthritis of limbs affected obese women and was dominated by lower limbs. The knee remains its most frequent localization
Étude ethnomédicinale des plantes utilisées dans le traitement de quelques maladies du foie dans la sous-préfecture de Bengassou (Centre-Est de la Côte d’Ivoire)
Get PDFLes maladies du foie peuvent constituer une principale source de menace pour la santé publique. En Afrique, la situation est plus complexe en raison des contaminations par les virus des hépatites B et C. En Côte d’Ivoire, la prévalence des hépatites est de 12 % pour l’hépatite virale B et de 5 % pour l’hépatite virale C. Le traitement médical constitue l’un des défis majeurs à relever pour les vaincre. Cependant, le coût élevé du traitement proposé par la médecine moderne, oblige la plupart des patients à recourir à la médecine traditionnelle. Il est donc nécessaire d’explorer le monde végétal pour rechercher les plantes médicinales pouvant constituer une alternative thérapeutique non négligeable. L’objectif de cette étude est d’inventorier les plantes médicinales utilisées dans le traitement traditionnel des atteintes hépatiiques à Bengassou dans le département de Bocanda (Centre-Est de la Côte d’Ivoire). Les enquêtes ethnobotaniques ont été menées dans cette localité auprès de 23 tradipraticiens, à l’aide d’un questionnaire. Dans cette étude, trois pathologies du foie que sont l’ictère, l’hépatite et la cirrhose ont été recensées. Quarante-quatre (44) espèces végétales ont été répertoriées avec leurs modes d’utilisation. Les feuilles (76 %) constituant la partie de plante la plus utilisée, sont couramment préparées sous forme de décoction (82 %). La voie orale (50 %), reste principalement celle conseillée pour l’administration des remèdes. Les investigations sur l’implication des plantes médicinales dans les recettes contre ces affections ont montré que Alchornea cordifolia (Cpr= 17,65 %), Costus afer (Cpr= 11,76 %), Morinda lucida (Cpr= 11,76 %), Newbouldia leavis (Cpr= 11,76 %) et Vernonia colorata (Cpr= 11,76 %) entrent dans la plupart des préparations médicamenteuses. Selon ces résultats, les plantes recensées pourraient être utiles dans la lutte contre certaines atteintes hépatiques, particulièrement l’hépatite, la cirrhose et l’ictère.
Liver disease can be a major threat to public health. In Africa, the situation is more complex due to contamination by hepatitis B and C viruses. In Côte d'Ivoire, the prevalence of hepatitis is 12% for viral hepatitis B and 5% for viral hepatitis C. Medical treatment is one of the major challenges in overcoming them. However, the high cost of treatment offered by modern medicine forces most patients to turn to traditional medicine. It is therefore necessary to explore the plant world in search of medicinal plants that could provide a significant therapeutic alternative. The aim of this study is to inventory the medicinal plants used in the traditional treatment of hepatitis in Bengassou in the Bocanda department (central-eastern Côte d'Ivoire). Ethnobotanical surveys were carried out in this locality among 23 traditional practitioners, using a questionnaire. In this study, three liver pathologies were identified: jaundice, hepatitis and cirrhosis. Forty-four (44) plant species were listed, along with their modes of use. Leaves (76%), the most widely used part of the plant, are commonly prepared as a decoction (82%). The oral route (50%) remains the preferred method for administering remedies. Investigations into the involvement of medicinal plants in recipes to treat these ailments showed that Alchornea cordifolia (Cpr= 17.65%), Costus afer (Cpr= 11.76%), Morinda lucida (Cpr= 11.76%), Newbouldia leavis (Cpr= 11.76%) and Vernonia colorata (Cpr= 11.76%) are used in most medicinal preparations. According to these results, the plants listed could be useful in combating certain liver disorders, particularly hepatitis, cirrhosis and jaundice
Utilisation régulière du préservatif par les couples sérodiscordants au VIH au Bénin
Get PDFL’utilisation du préservatif au sein des couples sérodiscordants au VIH est une stratégie de prévention efficace et largement recommandée, en complément aux autres méthodes de prévention. L’objectif de cette étude était d’une part de déterminer la prévalence et les facteurs associés à l’utilisation régulière du préservatif masculin au sein des couples sérodiscordants et d’autre part de décrire les raisons qui motivent l’adoption ou non de ce comportement. Elle s’est déroulée sur les sites de prise en charge (PEC) de la ville de Parakou, au nord-est du Bénin. Il s’est agi d’une étude transversale multicentrique mixte. Les participants répondant aux critères d’inclusion ont été recrutés sur une période de 7 mois, allant d’août 2022 à février 2023. Sur la base d’un échantillonnage non probabiliste par commodité, les données quantitatives sociodémographiques, économiques, comportementales de même que les antécédents ont été recueillies à partir d’un questionnaire. Le volet qualitatif a permis de recueillir les perceptions et motivations des enquêtés, dans le but purement descriptif, afin d’expliciter les données recueillies par l’enquête quantitative. L’analyse et le traitement des données ont été faits avec le logiciel SPSS 25. Le seuil de significativité a été fixé à 5%. La régression logistique a été utilisée pour identifier les facteurs associés. Les données qualitatives, après transcription, ont bénéficié d’une analyse de contenu thématique. L’utilisation régulière du préservatif concernait moins de 5% des enquêtés. Dans cette population d’utilisateurs réguliers du préservatif, les jeunes (71,43%) et les femmes (85,71%) étaient les plus représentés. La plupart d’entre eux (71,43%) avaient un niveau d’instruction secondaire et plus, avaient un revenu inférieur à 50.000 francs CFA (64,28%), étaient mariés (85,71%) et polygames dans 57,14% des cas. Près de 29% des sujets utilisant systématiquement le préservatif dans leur couple étaient testés positifs pour l’hépatite B et 21,43% démontraient une mauvaise observance du traitement antiretroviral (TAR). Les facteurs associés à l’utilisation régulière du préservatif étaient : l’attitude des personnes vivant avec le VIH (PVVIH) face à la maladie (p = 0,039) et le stade clinique de l’infection (p = 0,007). Les facteurs facilitant l’utilisation régulière du préservatif étaient : une utilisation du préservatif antérieure au dépistage positif, les recommandations insistantes des agents de santé, la gratuité des préservatifs, la communication saine sur le VIH dans le couple, et les menaces des conjoints. Quant aux barrières retrouvées, il s’agissait de : l’absence de partage du statut sérologique avec le conjoint, la mauvaise perception de l’usage du préservatif dans un couple stable, le déni de la maladie ou la banalisation du risque de transmission et le besoin de prouver à l’autre son amour et sa confiance. Des actions de sensibilisation doivent être menées à l’endroit de la population générale afin d’améliorer en amont de l’infection la perception de l’utilisation du préservatif au sein du couple.
Condom use in HIV-serodiscordant couples is an effective and widely recommended prevention strategy, complementing other prevention methods. The aim of this study was to determine the prevalence and factors associated with regular condom use among serodiscordant couples, and to describe the reasons for adopting or not adopting this behavior. It was carried out at care sites in the city of Parakou, in northeastern Benin. It was a mixed quantitative and qualitative multicenter cross-sectional study. Participants meeting the inclusion criteria were recruited over a 7-month period from August 2022 to February 2023. Based on non-probability sampling for convenience, quantitative socio-demographic, economic, behavioral and background data were collected using a questionnaire. As for the qualitative component, the aim was to collect the perceptions and motivations of the respondents, for descriptive purposes, in order to clarify the data collected by the quantitative survey. Data were analyzed and processed using SPSS 25 software. The significance level was set at 5%. Logistic regression was used to identify associated factors. After transcription, qualitative data were subjected to thematic content analysis. Regular condom use concerned less than 5% of respondents (4.68%). In this population of regular condom users, young people (71.43%) and women (85.71%) were the most represented. Most of them (71.43%) had a secondary education or higher, had an income of less than 50,000 CFA francs (64.28%), were married (85.71%) and polygamous in 57.14% of cases. Nearly 29% of subjects who systematically used condoms in their relationship tested positive for hepatitis B, and 21.43% showed poor compliance with ART. Factors associated with regular condom use were: Persons living with HIV's attitude to the disease (p = 0.039) and clinical stage of infection (p = 0.007). Factors facilitating regular condom use were: habitual condom use prior to positive testing, insistent recommendations from health workers, free condoms, healthy communication about HIV within the couple, and threats from HIV-negative spouses. As for the barriers found, these were: failure to share serostatus with the partner, bad perception of condom use in a stable couple, denial of the disease or trivialization of the risk of transmission, and the need to prove love and trust to the partner. Public awareness campaigns are needed to improve the general perception of condom use within couples